• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.335-339
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• 1998

The factors affecting the isolation and culture of the protoplast of embryogenic callus, derived from immature ovule in Citrus junos, were examined. An incubation time in enzyme solution of 16 hrs was preferable for protoplast isolation. Efficient protoplast yields were obtained from the treatment of equal concentration of 0.7 M $\textrm{BH}_{3}$ to the enzyme solution containing 1.0% cellulase, 1.0% macerozyme and 0.2% pectolyase. Protoplast cultured in MT medium with 0.1 mg/L 2,4-D showed vigorous division and some of them formed callus. Induced callus was subcultured on solid MT medium but the callus showed very slow growth. The above results show the possibility to culture from protoplast fusion in Citrus genera.

• The Korean Journal of Zoology
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• v.34 no.3
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• pp.352-367
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• 1991

척추동물 11목 21종의 췌장에서 insulin(B)세포, glucagon(A)세포, somatostatin( D)세포 및 pancreatic polypeptide(PP)세포 등을 면역세포화학적 방법으로 동정하여 이들의 출현율, 분포양상 및 형띨 등을 계통별로 비교하였다. 포유강에서는 전형적인 췌도를 형성하였고, 조강에서는 대 ·소췌도로 구분되었다. 내분비세포의 크기는 계통간의 차이가 있어서 B세포와 PP세포는 포유강의 것이 조강의 것에 비해 윤으나, A세포와 D세포는 그 반대이었다. 세포의 모양은 B세포의 경우 두 강에서 대체로 원형 및 난원형이었고, A세포와 D세포는 원형, 난원형 및 방추형이었다. PP세포는 방추형 및 난원형이 대다수 이었으며 간혹 원형 또는 다각형 등의 모습도 나타났다. 세포들의 출현율은 두 강에서 모두 B세포가가장 많았고, A, D및 PP세포 순으로 낮아졌다. B세포는포유강이 조강보다높았고, A, D및 PP세포는조강이 포유강보다 많았다. B세포는 조강의 경우 대췌도 주변부와 소췌도 중앙부에 분포하였고, 포유강에서는 중앙부에 균등하게 분포하였다. A와 0세포는 포유강에서 주로 췌도 주면부에 분포하나, 조강에서는 췌도중앙부에 위치하였다. PP세포는 두 강에서 대부분 췌도 주변부에 위치하였다. 일반적으로 두강에서 모두 외분비 선포조직에서도 내분비세포들이 출현하였으며 일부 종에서는 췌관상피에서도 내분비세포들이 드물게 나타났다.

• Korean Journal of Optics and Photonics
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• v.17 no.6
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• pp.519-524
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• 2006

In general, the Lohmann-type binary hologram represents its amplitude and phase by using the rectangular cell. In this paper, we adapts a circular cell to represents the amplitude and phase of holograms. In order to compare the characteristics of the circular cell with the rectangular one, we analyzed the results based on the computer simulations and various optical experiments. The results show that a clearer reconstructed image can be obtained by dividing one cell into many pixels. In the case of a uniform reconstructed image, the rectangular cell is better than the circular cell. However, as for the brightness of the reconstructed image, the circular cell is better than the rectangular one.

• Korean Journal of Microbiology
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• v.24 no.3
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• pp.197-203
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• 1986

Morphology and ultrastructure of protoplast fusion mode in Streptomyces lavendulae were studied by scanning and transmission electron microscopy. The isolated protoplasts were stable in some degree in hypertonic solution except that several protoplasts showed irregular morphology. Fusion events were occurred as follows; contact zone, fusion zone and separation zone were appeared sequentially. After formation of the separation zone, cytoplasm and DNA from both parents were mixed eventually. In the contact zone, two menbranes were still separated by electron transparent space. The contact zone changed to fusion zone by formation of fusion membrane that phospholipid molecules of two membranes were rearranged. Thereafter, nonmembraneous separation zone was formed by disappearance of fusion membrane. These changes were characterized by successive changes in typical membrane structure in fusion areas and by a progressive loss of bispherical shape.

• Journal of the Korean Society of Clothing and Textiles
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• v.25 no.10
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• pp.1754-1762
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• 2001

본 연구의 목적은 적합성과 기능성이 고려된 체형별 남성 상의 원형 패턴을 개발하는데 있다. 피험자는 인체계측자료와 측면체형 사진에 의해 선정하고, 체형은 굴신체형, 표준체형, 반신체형으로 분류하였다. 체형별 실험 원형 패턴의 평가를 위하여 기존원형을 선정하였으며, 연구 결과는 다음과 같다; 1. 착의평가 결과 표준체형은 대부분의 항목에서 적합하였으나, 굴신체형과 반신체형에서는 많은 차이가 있었다. 이러한 차이는 앞길이와 등길이 뿐만 아니라 여유량과 윗 가슴둘레에 서 나타난다. 앞품과 뒤품은 외관에 의해 많은 영향을 받으며, 증가된 옆품은 앞품과 뒤품의 부족분을 보충하는 역할을 하였다. 따라서 실험패턴은 기존패턴보다 각 체형에 더욱 적합하며, 체형 적합성과 동작기능성이 고려된 의복 제작을 위한 패턴설계는 인체의 구조와 동작연구에 의해 가능하였다. 2. 기본원형 패턴 설계를 위한 필요치수는 목뒤높이, 등길이, 앞길이, 윗 가슴둘레, 앞품과 뒤품이며, 체형분류의 기준은 윗 가슴둘레, 앞품, 뒤품, 앞길이, 등길이이다. 이와 같은 결과를 근거로 가슴둘레 선은 [(B/2+B/10), 뒤진동깊이는 [(B/10+목뒤높이/10)]로 설정하고, 앞내림은 1.5cm로 하였다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.3
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• pp.554-559
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• 2009

This paper proposes a new method of weighted template matching fur machine-printed numeral recognition. The proposed weighted template matching, which emphasizes the feature of a pattern using adaptive Hamming distance on local feature areas, improves the recognition rate while template matching processes an input image as one global feature. The experiment compares confusion matrices of the template matching, error back propagation neural network classifier, and the proposed weighted template matching respectively. The result shows that the proposed method improves fairly the recognition rate of the machine-printed numerals.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.400-400
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• 2016

자연하천에서 다양한 목적으로 설치된 수공구조물을 볼 수 있으며 대표적으로 교량의 교각을 들 수 있다. 교각 주위에서 발생하는 국부세굴은 교량붕괴의 가장 큰 원인이므로 이에 대한 연구는 필수적이다. 수리실험 또는 수치모형을 이용하여 교각주변의 국부세굴에 관한 많은 연구들이 수행되었지만 세굴인자의 특성 및 메커니즘에 대한 연구는 여전히 부족하다. 본 연구에서는 LES(large-eddy simulation)에 유사이송 및 하상변동 모형과 결합하여 2개의 원형교각 주변의 흐름 및 세굴을 수치모의 하였다. LES와 유사이송 및 하상변동 모형의 결합은 난류의 영향을 직접 모형에 고려할 수 있기 때문에 교각 주변에서 발생하는 말굽형 와 구조와 같은 복잡한 흐름에 의한 영향이 반영된다. 계산영역은 흐름방향으로 10 m, 횡 방향으로 2.4 m로 하였고, 지름(D) 0.16 m를 가지는 원형교각을 유입부로부터 2.4 m 떨어진 위치에 배치하였다. 이때 두 개 교각사이의 각은 $0^{\circ}{\sim90^{\circ}$이고 원형교각 사이의 거리는 5D로 하였다. 수치모의에 사용된 조건은 이전의 수리실험(Khosronejad et al., 2012)을 참고하여 접근평균유속은 0.25 m/s, 수심은 0.15m를 사용하였다. 수치모의는 원형교각 주변의 최대세굴심이 평형상태에 이를 때 까지 수행하였다. 접근각도 변화에 따른 원형교각 주변의 세굴과정 및 특성을 분석하였으며 최대 세굴심 결과를 Hannah(1978)의 수리실험결과와 비교하였다.

• Journal of Korea Entertainment Industry Association
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• v.15 no.7
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• pp.259-269
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• 2021

This study examines the legend of 'Grandmother Seolmundae and Five Hundred Generals', the cultural archetypes of Jeju, and analyzes the characteristics of its contents. After analyzing the feasibility of using the analyzed cultural prototype as cultural contents such as games and animations, based on this analysis, we tried to suggest a cultural planning approach based on the cultural prototype to the cultural agency. Therefore, the implications of this study are as follows. First, among the cultural prototypes in Korea, 'Grandmother Seolmundae and Five Hundred Generals', which represent the legends of Jeju Island, are being organized centered on many historical researchers and Chaerokga, but there is no precise establishment of the exact timing of the legend and how it arose. Therefore, when planning and developing content based on the cultural prototype, it is most important for cultural agencies to develop a story after researching historical evidence and opinions of local residents to identify a consistent point of view. Second, although the contents of the cultural archetype are arranged slightly differently by historians and recorders, the main contents and characteristics of the story are found to have a similar form. Therefore, cultural agencies should focus on finding the point of view and characteristics of a story, even if a story is written differently by different people when doing a cultural prototype. Third, when planning a game based on the cultural prototype, the main elements such as the elements to be expressed in the game and the fun elements should be found and presented. In particular, because fun and rules are the most important parts of games, if this part cannot be derived from the story of the cultural archetype or cannot be made, it is difficult to transform the cultural archetype into a game. Therefore, it can be seen that it is important for cultural agencies to set their game plan intentions in consideration of story expression and fun, even if it is the core or non-core of the entire story of the cultural archetype. Lastly, although the cultural prototype 'Grandmother Seolmundae and Five Hundred Generals' was presented as animation content, it is important to develop it considering the story, characters, media, and audience. Therefore, cultural agencies should be able to derive the elements such as stories, representative and auxiliary characters, and viewers that can be adapted from the cultural prototype as much as possible. It will be an important part of raising.

• Korean Journal of Animal Reproduction
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• v.22 no.4
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• pp.395-403
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• 1998

In this study we determined fertilization processes and developmental ability of porcine oocytes following injection of round spermatid in the presence and absence of artificial activation. Electrical stimulation at 2 h before spermatid injection significantly increased the incidence of normal fertilization as compared to those following injection without stimulation or with stimulation immediately after injection. The incidences of two pronuclear formation and apposition were not different in oocytes between following intracytoplasmic spermatid and spermatid nucleus injection. Indirect immunocyto chemistry and laser scanning confocal microscopy study revealed that microtubuels were organized from the oocyte cortex following round spermatid injection, and this seemed to move both male and female pronuclei into the oocyte center. At 6 days following injection blastocoele formation was seen in the eggs following round spermatid (25%) and round spermatid nucleus injection (27%). However, none of oocytes developed to the blastocyst stage at 6 days following sham injection. The average cell numbers of blastocysts at 8 days following injection of spermatid and spermatid nucleus were 87 to 99. These results suggested that either round spermatid or it's nudeus can be used to produce viable embryos by injection into unfertilized oocytes in the pig.

• Journal of Korean Society of Steel Construction
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• v.21 no.5
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• pp.505-514
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• 2009

This paper describes the structural behavior and the ultimate strengths of circular hollow steel (CHS) sections based on a series of compression tests. The ultimate strengths of CHS section columns are mainly dependent on both diameter-thickness ratio and column slenderness ratio. For the CHS sections with a high diameter-thickness ratio, an elastic or an inelastic local buckling may occur prior to the overall buckling, and it may decrease the column strength. Test sections were fabricated from SM400 steel plate of 2.8 mm and 3.2 mm in thickness and were tested to failure. The diameter-thickness ratios of the test sections ranged from 45 to 170 to investigate the effect of local buckling on the column strength. The compression tests indicated that the CHS sections of lower diameter-thickness ratio than the yield limit in the current design specifications showed an inelastic local buckling and a significant post-buckling strength in the local mode. Their ultimate stresses were larger than the nominal yield stress. It was known that the allowable stresses of the sections predicted by the Korean Highway Bridge Design Specifications (2005) were too conservative in comparison with test results. The Direct Strength Method which was newly developed was calibrated for application to the CHS sections by the experimental and numerical results. The Direct Strength Method proposed can predict properly the ultimate strength of CHS section columns whether a local buckling and an overall buckling occur nearly simultaneously or not.