• Horticultural Science & Technology
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• v.33 no.5
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• pp.737-750
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• 2015

This study was carried out to construct a DNA profile database for 102 watermelon cultivars through the comparison of polymorphism level and genetic relatedness using genomic microsatellite (gMS) and expressed sequence tag (EST)-microsatellite (eMS) markers. Sixteen gMS and 10 eMS primers showed hyper-variability and were able to represent the genetic variation within 102 watermelon cultivars. With gMS markers, an average of 3.63 alleles per marker were detected with a polymorphism information content (PIC) value of 0.479, whereas with eMS markers, the average number of alleles per marker was 2.50 and the PIC value was 0.425, indicating that eMS detects a lower polymorphism level compared to gMS. Cluster analysis and Jaccard's genetic distance coefficients using the unweighted pair group method with arithmetic average (UPGMA) based on the gMS, eMS, and combined data sets showed that 102 commercial watermelon cultivars could be categorized into 6 to 8 major groups corresponding to phenotypic traits. Moreover, this method was sufficient to identify 78 out of 102 cultivars. Correlation analysis with Mantel tests for those clusters using 3 data sets showed high correlation ($r{\geq}0.80$). Therefore, the microsatellite markers used in this study may serve as a useful tool for germplasm evaluation, genetic purity assessment, and fingerprinting of watermelon cultivars.

• Journal of agriculture & life science
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• v.43 no.2
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• pp.47-53
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• 2009

This research is intended to study the induction net of chrysanthemum used in its greenhouse and requiring lots of time for manual work and review the structural safety of a bed and existing greenhouses after designing the bed of a net which increases cropping period by sharply reducing the time of work and provides the bed of induction nets for cultivating chrysanthemum with its existing and new greenhouse. The review of sectional and biodynamic properties in 15 kinds of materials has revealed that the pipes of ${\phi}38.1{\times}1.7t$and ${\phi}38.1{\times}2.0t$ didn't exceed stress ratio but did 10mm drooping allowance. For this reason, the pipe of ${\phi}48.1{\times}1.5t$ net both stress ratio and drooping allowance. For the safety, the middle chamber should be designed into Truss type owing to bed load, wind load, and snow load when the bed of an induction net is installed in the middle chamber. When installing the middle chamber with a truss type, the greenhouse of chrysanthemum in Geochang area needs proper reinforcements because the stress in colullllls and wind proof walls exceeds stress allowance regardless of the installation of a bed.

• Korean Journal of Agricultural and Forest Meteorology
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• v.21 no.4
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• pp.373-379
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• 2019

Smart-farm has been spreading across Korea to improve the labor efficiency and productivity of greenhouse crops. Although notable improvements have been made in the monitoring technologies and environmental-controlling systems in greenhouses, only a few simple decision-support systems are available for predicting the optimum environmental conditions for crop growth. In this study, a tomato growth model (GreenTom), which was developed by Seoul National University in 1997, was calibrated and validated to examine if the model can be used as a decision-supporting system. The original GreenTom model was not able to simulate artificial defoliation, which resulted in overestimation of the leaf area index in the late growth. Thus, an algorithm for simulating the artificial defoliation was developed and added to the original model. The node development, leaf growth, stem growth, fruit growth, and leaf area index were generally well simulated by the modified model indicating that the model could be used effectively in the decision-making of smart greenhouse.

• Journal of Industrial Convergence
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• v.19 no.5
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• pp.91-102
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• 2021

The purpose of this study was to systematically review about randomized controlled trials the characteristics and effect of cognitive function intervention for patient with dementia. We searched studies published from January 2010 to June 2021 in 5 databases. A total 1,104 studies were found and included 27 studies in final analysis. Methodological quality was assessment with the Cochrane's RoB(risk of bias) tool. Mini-Mental State Examination(MMSE) was the most used as the assessment tool for identifying the cognitive function. Cognitive function intervention were exercise, art, cognitive stimulation, reminiscence, music, multimodal cognitive rehabilitation, virtual reality, horticultural, computerized cognitive training, intentional snoezelen, beauty, cooking, korean traditional familiarity program. Most of the intervention except exercise 2, virtual reality 1, beauty 1 were effective in improving cognitive function. This study provided a clinical evidence for planning and implementing intervention for cognitive function intervention. In the future, various intervention studies suitable for the characteristics of dementia should be conducted by improving the quality of research methods.

• Journal of Practical Agriculture & Fisheries Research
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• v.13 no.1
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• pp.47-69
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• 2011

The purpose of this study is to suggest strategies for vitalizing Jeju citrus export-specialized organization. To achieve the goal, the research has been focused on the establishment and standardization for fruit production practices that can keep a steady supply of high quality citrus fruit for export market, and followed by systematization of farms using the practices. According to the research findings, it is desirable that the high quality fruit production manual such as the Global GAP must be established, developed and prepared to train farm households. The establishment of the high quality fruit production manual is focused on the items as follows : 1) labor productivity enhancement by structural improvement of farm layout, 2) the practices for high-sugar-fruit production such as thinning out, irrigation and drainage system, and mulching with a poromeric sheet 3) productivity enhancement and quality improvement of fruit by cultivar improvement and virus-free nursery trees, 4) standardization of cultural practices throughout the citrus fruit production.

• Journal of Mushroom
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• v.16 no.4
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• pp.250-256
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• 2018

The windrow turner, widely used for outdoor fermentation of mushrooms in Europe, has been improved by using rice straw instead of wheat straw in accordance with Korea's actual situation. It was compared with conventional excavator work, and the results were as follows. Agitation performance was $81m^3/hr$, which was 2.8 times higher than $28.6m^3/hr$ of excavator. As a result of the temperature distribution in the pile at the end of the fermentation stage, it was found that the temperature at the lower part of the pile was $5^{\circ}C$ higher than that of conventional pile. This was more favorable for aerobic fermentation. Meanwhile, ash ratio of prototype ($30.9{\pm}1.1%$) was higher than that of control $28.4{\pm}1.6%$. In the case of prototype turner agitation, the yield of mushroom cultivation was $880kg/66m^2$, that was 22.9% higher than the conventional control yield of $716kg/66m^2$.

• Journal of the Korean Society of Floral Art and Design
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• no.45
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• pp.47-56
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• 2021

It was applied by making the models such as the prior learning (e-learning), modeling by manual, learner's practice, 1:1 teaching coaching, self evaluation, coaching behavior assessment(primary, secondary), and self-directed practice. First, the cognitive practice education through the prior learning is very essential in the practice of floral design. Second, the practice class of floral design is a class where the professor generally set an example first, and the learners followed. Third, this study was to prepare the checklist, reflect it through the self evaluation, and prepare the evaluation form in accordance with the element, principle, and technical parts of floral design about the finished works. Fourth, contrary to the existing class completing within the class hour, the practice class is a process of trying to do self-directed practice, returning to home. Fifth, this study was to evaluate the works the learner made once again through the sketching and photographing by placing the work process of portfolio at the last step. To conclude, this study has found that such series of process through six steps on the practice form by the learner only would be excellent teaching learning model to improving the basic capacity of floral design. Accordingly, the development of teaching materials related to this and adaptation in the field in the future is considered as it will be very helpful to the learners' self-directed learning.

• Journal of Food Hygiene and Safety
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• v.34 no.1
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• pp.40-51
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• 2019

Ginseng has a unique production system that is different from those used for other crops. It is subject to the Ginseng Industry Act., requires a long-term cultivation period of 4-6 years, involves complicated cultivation characteristics whereby ginseng is not produced in a single location, and many ginseng farmers engage in mixed-farming. Therefore, to bring the production of Ginseng in line with GAP standards, it is necessary to better understand the on-site practices of Ginseng farmers according to established control points, and to provide a proper action plan for improving efficiency. Among ginseng farmers in Korea who applied for GAP certification, 77.6% obtained it, which is lower than the 94.1% of farmers who obtained certification for other products. 13.7% of the applicants were judged to be unsuitable during document review due to their use of unregistered pesticides and soil heavy metals. Another 8.7% of applicants failed to obtain certification due to inadequate management results. This is a considerably higher rate of failure than the 5.3% incompatibility of document inspection and 0.6% incompatibility of on-site inspection, which suggests that it is relatively more difficult to obtain GAP certification for ginseng farming than for other crops. Ginseng farmers were given an average of 2.65 points out of 10 essential control points and a total 72 control points, which was slightly lower than the 2.81 points obtained for other crops. In particular, ginseng farmers were given an average of 1.96 points in the evaluation of compliance with the safe use standards for pesticides, which was much lower than the average of 2.95 points for other crops. Therefore, it is necessary to train ginseng farmers to comply with the safe use of pesticides. In the other essential control points, the ginseng farmers were rated at an average of 2.33 points, lower than the 2.58 points given for other crops. Several other areas of compliance in which the ginseng farmers also rated low in comparison to other crops were found. These inclued record keeping over 1 year, record of pesticide use, pesticide storages, posts harvest storage management, hand washing before and after work, hygiene related to work clothing, training of workers safety and hygiene, and written plan of hazard management. Also, among the total 72 control points, there are 12 control points (10 required, 2 recommended) that do not apply to ginseng. Therefore, it is considered inappropriate to conduct an effective evaluation of the ginseng production process based on the existing certification standards. In conclusion, differentiated certification standards are needed to expand GAP certification for ginseng farmers, and it is also necessary to develop programs that can be implemented in a more systematic and field-oriented manner to provide the farmers with proper GAP management education.

• Horticultural Science & Technology
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• v.28 no.6
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• pp.1025-1038
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• 2010

Carrot ($Daucus$ $carota$ L. var. $sativa$) is one of the most widely used crops in the world. Moreover it is an important crop because of its high content of ${\beta}$-carotene, well-known as the precursor of vitamin A carotenoid. However, seed-hair which is generated in epidermal cell of seeds inhibits absorption and germination. For that reason, carrot seeds are commercialized after mechanical hair removal process. To overcome such cumbersome weaknesses, new breeding program for developing hairless-seed carrot cultivar has been needed. Therefore, in this study, cDNA libraries from seeds of short-hair seed phenotype CT-ATR615 OP 666-13line and hairy seed CT-ATR615 OP-CK1-9 line were constructed and expression patterns related to generation of seed-hair were analyzed by comparison of EST sequences. Differential EST sequence results between two lines were classified into FunCat functional categories based on the results of BlastX search. Higher expression quantities belonging to metabolic category were shown on short-hair seed line than hairy-seed one. Differential expression quantities between those two lines in the protein folding and stabilization, subcellular localization categories were supposed to contribute variously on the generation of seed-hair. We confirmed 50 and 59 SSR sites, and 2 SNP sites by analyzing EST sequences in two lines; thereafter, we designed SNP and SSR primer sets from these EST sequence information as a molecular marker. These markers are thought to be used in research of molecular markers for classification of carrot family and related to various traits, as well as seed-hair characteristic.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.378-383
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• 2001

Embryo culture, ovule culture and ovary slice culture were tested to find optimum method for overcoming post fertilization barrier in interspecific crosses between L. longiflorum 'Gelria' and L. cernuum. Although reciprocal crosses between the species were carried out by cut-style pollination method, fruits developed only in crosses of L. longiflorum${\times}$L. cernuum. On the 40 days after pollination, ovaries were sliced into 2-4mm thickness and cultured on a hormone-free Murashige-Skoog (MS) medium, supplemented with 2%, 4%, 6%, 8% and 10% sucrose. For the L. longiflorum Gelria'${\times}$L. cernuum cross, ovule development was found to be best at 6% sucrose and a lot of hybrid plant lets established directly from the ovary slice culture and subsequent ovule culture. High concentration of sucrose above 8% made ovules abort or vitrificate from 40 days after culture. In contrast, ovules from the L. cernuum${\times}$L. longiflorum 'Gelria' cross swelled well in ovary slice culture, however, they did not germinated in subsequent ovule culture. On the 60 days after pollination, ovules thicker than 0.6mm was interpreted as one containing embryo. The embryo size ranged from 1.2 mm to 1.7 mm, and in vitro development of the excised embryos was found to be best with the MS medium (pH 5.8), supplemented with $0.1-1 mg{\cdot}L^{-1}$ NAA and 6% sucrose. Thick ovules excised 60 days after pollination germinated about 60% as normal seeds in MS medium supplemented with 6% sucrose and free hormone. The ovule culture 60 days after pollination was concluded to be most recommendable to produce interspecific hybrids in large scale crosses between L. longiflorum 'Gelria' and L. cernuum by the reason of easy procedure.