• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.3
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• pp.307-311
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• 2014

2,3,5-Triphenyltetrazolium chloride (TTC) is used as a redox indicator in culture media. It is colorless in the oxidized form and is reduced to formazan, an insoluble pigment, by dehydrogenases in actively growing microbial cells. The aim of this study was to assess by microbial test of the pathogenic microorganisms using TTC reduction. The pathogenic microorganisms were reduced in medium by dehydrogenase to produce insoluble red formazan. We observed that the optimization method of TTC allowed more than 12 h incubation in 0.04% concentration. Also, the growth of microorganisms with media was increased formazan production. We confirmed that microorganisms were quickly observed to grow colonies cultured red color without affecting the growth of microorganisms. It is suggested that the microbial test using TTC can provide better and quicker test method in cosmetics development.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.5
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• pp.326-334
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• 2012

To determine the concentrations and the mass flow of selected 10 perfluorinated compounds (PFCs), a field study was conducted in a wastewater treatment plant. Raw influent, primary influent, primary effluent, aeration tank effluent, secondary effluent, final effluent, dehydration liquor, primary sludge, thickened sludge, final sludge were collected over 3 days in the summer and the winter respectively. Collected samples were equally mixed and then served as an analytical sample. Total 10 compounds were analyzed. In terms of treated water, the concentration of perfluorooctanesulfonate (PFOS) and perfluorooctanoate (PFOA) were in range of N.D.~26.29 ng/L and N.D.~38.15 ng/L respectively. Perfluorononanoate (PFNA) and perfluorohexanesulfonate (PFHxS) were ranged from N.D. to 36.79 ng/L and from N.D. to 24.36 ng/L. In terms of sludges, a concentration of PFOS, PFOA, and perfluorodecanesulfonate (PFDS) were detected from 6.82 to 59.37 ng/g, from 0.13 to 0.37 ng/g, from N.D. to 0.83 ng/g respectively. Mass loading for PFCs increased during wastewater treatment except for PFNA. The observed increase in mass flow of PFCs may have resulted from biodegradation of precursor compounds.

• Korean Chemical Engineering Research
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• v.45 no.4
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• pp.328-334
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• 2007

Chemical heat pump system of 2-propanol/acetone/hydrogen is most suitable to the recovery of waste heat of power plant. various types of 5 wt% Pt-alumina catalysts were prepared for 2-propanol dehydrogenation using sol-gel method. The characteristics and the dehydrogenation reaction rate of each catalyst were investigated. Pt-alumina xerogel catalyst has excellent reaction rate and good durability in comparison with the existing alumina supported Pt catalysts. Pt-alumina aerogel catalyst had the highest reaction rate in all prepared catalysts, but sufficient aging time was necessary to maintain its reaction rate. A potential advantage of the aerogel catalyst is the fact that the high temperature heat treatment is not required. Without heat treatment or with low temperature heat treatment, the Pt-alumina aerogel catalyst has excellent reaction rate as well as durability and this gives us the economic advantage. Alumina xerogel supported Pt catalyst prepared by incipient wetness method showed good reaction rate, and had good mechanical strength. Blank alumina xerogel prepared by sol-gel method can be used for the support of metal catalysts.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.1
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• pp.15-24
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• 2010

This study was conducted to investigate the effects of swine manure (SM) application with additional nitrogen (N) fertilizer on productivity of corn and environmental pollution in com cultivation soil. The experiment was conformed in lysimeter which was constructed with 30 cm diameter, and 100 cm height. Swine manures used in this study were the compost of swine manure fermented with sawdust (SMFWS) and soft rice hulls treated with high temperature and high pressure (SRH), and swine slurry (SS). The application rates of the additional N, as urea, with swine manure were 50 and 100 kgN/ha on each plot. This study was arranged in completely randomized design with three replication. DM yields in SM treatments with mineral N were increased significantly compared to those in SM treatment without mineral N (zero-mineral N) (P<0.05) and increased as the rate of mineral N application increased (P<0.05). DM yields in SMFWS and SS treatments with mineral 100 kgN/ha showed trends similar to those of chemical fertilizer (control) but higher than those of 50 kg N/ha. DM yields tended to be higher in SMFWS and SS treatments than in SRH treatment. Total N contents in SMFWS, SRH and SS treatments with mineral N were increased, compared with SM treatment without mineral N. N contents in SM treatments with mineral N were lower than those of chemical fertilizer treatment, but N content of chemical fertilizer treatment showed trends similar to that of SS treatments with mineral 100 kgN/ha. $NO_3$-N concentration in SM treatments with mineral N were increased significantly, compared to those in SM treatment without mineral N and in chemical fertilizer (P<0.05). $NH_4$-N concentrations in SMFWS and SS treatments with mineral 100 kgN/ha showed trends similar to those of chemical fertilizer, but higher than those of 50 kg N/ha. $PO_4$-P concentration in SM treatments with mineral N were increased significantly, compared to those in SM treatment without mineral N (P<0.05). $PO_4$-P concentration in chemical fertilizer treatment showed trends similar to that of SS treatments with mineral 100 kgN/ha. The concentrations of $NO_3$-N $NH_4$-N and $PO_4$-P increased as the rate of mineral N application increased (P<0.05). The concentrations of $NO_3$-N $NH_4$-N and $PO_4$-P were highly elevated in the concentrated rainy season in the early stage among experimental period. The maximum $NO_3$-N $NH_4$-N and $PO_4$-P concentrations in the leaching water were 3.46 mg/L, 1.11 mg/L and 0.14 mg/L, respectively.

• Journal of the Korea Organic Resources Recycling Association
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• v.8 no.4
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• pp.93-99
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• 2000

Treatment of shaving scrap, a chrome containing solid scrap generated by leather manufacturing process, has been so far depended on mainly incineration, soil landfill and ocean dumping, which give bad impact on environment and cause pollution. Shaving scrap generates from the mechanical work for controlling the final thickness of leather and its main components are collagen protein and pan of chromium compound. For the purpose of reusing this leather waste as resources, researches in connection with collagen fiber recovery, gelable protein recovery and liquid fertilizer is being speedily progressed. In the experiment, shaving scrap went through wet pulverizing treatment by physical and chemical methods. Then, making the leather sheet evenly, it is mixed with natural latex and every kind of binding materials in the container, and the mixtures were passed through experimental hydraulic press machine and applied to Fourdrinier machine respectively. Lastly, a test for fading out physical strength and properties of multiple-purpose of leather-like material was performed on a continuous leather sheet prepared by the experiment. In result, the physical strength and properties of leather-like material showed noticeable differences according to mixing ratio of binding materials, beating methods and the Ends of binding materials selected, and generally tear strength was the weakest property among others. Also, by the pilot scale experiment in sequence, it was possible to manufacture recycled goods made of soft and hard types of leather-like material with various performances.

• Journal of Animal Environmental Science
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• v.12 no.1
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• pp.35-40
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• 2006

This experiment was conducted to investigate the effect of application level of swine slurry on the growth characteristics and yield of sorghum$\times$sudangrass hybrid and $NO_3-N$ content in infiltration at experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA from 2000 to 2002. Treatments were consisted of non fertilizer(NF), chemical fertilizer(CF), 100% swine slurry(SS 100), 150% swine slurry(SS 150), 200% swine slurry(SS 200) and 100% swine slurry + CF 50%(SS100 + CF 50) with randomized complete block design and three replications. Growth of sorghum$\times$sudangrass hybrid was not nearly different among the treatments, but early growth of swine slurry treatments was better than that of CF, and regrowth after 1st cutting was shown better in CF and SS 100+CF 50 with adding application of chemical fertilizer. The sugar content(brix %) was tends to be increased with swine slurry application. Dry matter(DM) yields of SS 100 and SS 150 were lower 15 and 6% than that of CF, respectively, and SS 200 was similer to CF, but there was not found significant difference among all treatments. The content of crude protein(CP), acid detergent fiber(ADF), and neutral detergent fiber(NDF) did not show the difference. The content of $NO_3-N$ in infiltration water was not more than CF by the 55 150 application, but more than by SS 200 and 55 100+CF 50 treatment.

• The Korean Journal of Pesticide Science
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• v.7 no.3
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• pp.176-181
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• 2003

The leaching behaviour of [aniline-$^{14}C$]mefenacet in soil was investigated using glass columns (5 cm I.D. $\times$ 30 cm. H) packed with two types of soils with different physicochemical properties. $^{14}C$-Mefenacet (8.33 kBq) and mefenacet (in total, 1.05 mg/kg) were treated onto soil columns and rice plants (Oryza sativa L.) were grown for 17 weeks on these columns. Leachates from the columns were collected at the rate of 122.5 mL per week. $^{14}C$-Activities leached from soil A (OM, 3.1%; CEC, 86 mmol(+)/kg; texture, loam) columns with and without rice plants were 1.95 and 4.19% of the originally applied, whereas those from soil B (OM, 1.3%; CEC, 71 mmol(+)/kg; texture, loam) were 2.69 and 7.05%, respectively. These results indicated that larger amounts of $^{14}C$ were percolated from soil B with less organic matter and from the columns without vegetation. $^{14}C$-Activities absorbed by rice plants from soil A and B were 8.95 and 8.47%, respectively, most of which remained in the root and shoot excluding unhulled grains and ears without grains. $62\sim73%$ of the originally applied $^{14}C$ remained in the depth of $0\sim5cm$ in soil. The mass balance indicated that the losses by volatilization and/or mineralization amounted to $3.4\sim9.2%$ of the originally applied. $^{14}C$-Radioactivities in the aqueous phase of the leachates ranged from 59.4 to 97.7% of the radioactivities in leachates, showing the fast transformation of mefenacet to the polar metabolites.

• Korean Journal of Food Science and Technology
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• v.16 no.1
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• pp.71-77
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• 1984

A study on the stability of chlorophyll a in Undaria pinnatifida during blanching, salting and storage was carried out. Raw Undaria pinnatifida was blanched for 25 seconds in the temperature range of 70 to $100^{\circ}C$. To stabilize the chlorophyll a some chemicals such as 1% solutions of $Ca(CH_3CO_2)_2,{\;}Ca(OH)_2,{\;}MgCO_3,{\;}0.5%{\;}Ca(CH_3CO_2)_2$ with $0.5%{\;}MgCO_3$, and reed ash solution were used during/after blanching. The blanched product was salted with table salt after centrifuging for 2 minutes at 1500 rpm, and then again centrifuged after 48 hours for dewatering. The product which was mixed with 8% of table salt was sealed in a polyethylene film bag and stored at 10, 20, 30 and $40^{\circ}C$. The most effective blanching temperature for maximal residual amount of chlorophyll a was $85^{\circ}C$. The quantities of total organic and volatile acids were not significantly changed by the blanching temperature. Blanching in 1% chemical solutions showed bitter results than soaking in 1% chemical solutions for 20 minutes after blanching without chemicals. Reed ash and 0.5% $Ca(CH_3CO_2)_2$ with 0.5% $MgCO_3$ solutions were more effective than the 1% solutions of other chemicals, but the effect was not significant, compared with the group not treated with chemicals. The most reasonable ratio of added salt to dewater the product for 48 hours was 30% in w/w. The amount of total organic and volatile acids revealed no correlation with the amount of added salt. Color and odor of salted product was not severely changed during the storage of 77 days at $10^{\circ}C$. But the changes were accelerated with increasing storage temperatures. The degradation of chlorophyll a in salted product during storage could be interpreted as a first order reaction, and the rate constants at 10, 20, 30 and $40^{\circ}C$ were 0.1289, 0.1028, 0.0770 and 0.0550, respectively. $Q_{10}$ and the activation energy were 1.33 and 5.01 Kcal/g mole.

• Journal of fish pathology
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• v.19 no.3
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• pp.253-265
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• 2006

The purpose of this study was to obtain reference data of parameters for hematological health diagnosis in marine fish and also evaluate application of veterinary chemistry analyzer used to those blood tests. A blood profile of total 522 fish for black rockfish (Sebastes schlegeli), red seabream (Pagrus major), rock bream (Oplegnathus fasciatu) and black seabream (Acanthopagrus schlegeli) cultured in mari-floating netcage of Gyeongnam province was determined by hematocrit (Ht), hemoglobin (Hb) and blood chemistry tests (total protein, albumin, alkaline phosphatase, blood urea nitrogen, lactate dehydrogenase, triglyceride, total cholesterol, creatinine, aspartate aminotransferase, alanine aminotransferase, glucose). Ht was measured by microhematocrit method. Hb and plasma chemistry were analysed by establishing baseline ranges for a dry chemical system of FUJI DRI-CHEM 3000. Actually recorded values of Hb and plasma chemistry by the analyzer were notably outside from the minimum and/or maximum of the established reference value. Albumin and alanine aminotransferase were not detectable in the range of 68～66%. Lactate dehydrogenase, total protein, alkaline phosphatase and glucose were not detectable in the range of 42～21%. Total cholesterol, aspartate aminotransferase, triglyceride, hemoglobin and creatinine were not detectable in the range of 18～3%. However, the values of blood urea nitrogen were below the detectable limits of the analyzer.

• Journal of the Korean Ceramic Society
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• v.38 no.3
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• pp.287-292
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• 2001

본 실험은 목적은 CRT(Cathode Ray Tube)용 청색 형광체인 ZnS:Ag 분말 표면에 액상법으로 SnO$_2$를 균일하게 코팅하는 공정조건을 연구하는 것이다. 용매로서 물을 사용하고, Sn의 공급물질로서 SnCl$_4$.4$H_2O$, 침전 촉매로서 CO(NH$_2$)$_2$를 각각 사용하여, 균일 침전 방법으로 ZnS:Ag 분말표면에 SnO$_2$를 코팅할 수 있었다. 초기에 첨가되는 SnCl$_4$.4$H_2O$의 량이 Sn/Zn의 몰비기준으로 0.017인 경우에 ZnS:Ag 분말표면에 Sn(OH)$_4$가 균일하게 코팅되지만, 그 이상 첨가되면 과량의 Sn(OH)$_4$가 입자들 사이에 응집되었다. 코팅된 Sn(OH)$_4$는 비정질 구조로 규명되었으며, 이를 SnO$_2$결정상으로 전이시키기 위하여 300~$700^{\circ}C$ 범위 내에서 열처리를 행하였다. 비정질 Sn(OH)$_4$는 20$0^{\circ}C$이하에서 탈수되었고 45$0^{\circ}C$부터 SnO$_2$로 결정화되기 시작하였다. 순수한 ZnS의 경우, 50$0^{\circ}C$이하에서는 상변화가 없으나, $600^{\circ}C$에서 일부 산화되었으며 $700^{\circ}C$에서는 완전히 ZnO로 산화되므로, ZnS의 산화방지 및 SnO$_2$의 결정화를 동시에 만족하는 최고 열처리온도는 50$0^{\circ}C$로 규명되었다. 그러나 ZnS에 SnO$_2$가 코팅된 시편의 경우에는 $600^{\circ}C$가 되어도 ZnS 상이 거의 산화되지 않았고, $700^{\circ}C$에서도 ZnS와 ZnO 상이 공존한 것으로 보아 SnO$_2$코팅이 ZnS의 산화를 억제하는 것으로 나타났다.