• Title/Summary/Keyword: 온도회복

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Long-Term Cold Storage and Plant Regeneration of Suspension Cultured Somatic Embryos of Eleutherococcus senticosus Maxim (가시오갈피 현탁배양 체세포배의 저온장기저장 및 식물체 재분화)

  • Li, Cheng Hao;Lim, Jung-Dae;Heo, Kwon;Kim, Myong-Jo;Lee, Chan-Ok;Lee, Jae-Geun;Cui, Xue Shu;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.6
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    • pp.494-499
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    • 2004
  • A method for long-term conservation of somatic embryos of Eleutherococcus senticosos was described. Suspension cultured globular somatic embryos were successfully conservated for 36 months at $4^{\circ}C$. The embryos resumed growth within two weeks when returned to MS liquid medium containing $0.2\;mg/{\ell}$. 2,4-dichlorophenoxy acetic acid. The optimal condition for cell proliferation was achieved when somatic embryos cultured at $32^{\circ}C$ in 1/3 MS liquid medium, and about 1.2 g of embryogenic cell was induced from 150 globular embryos after 6 weeks of suspension culture. The embryogenic cells produced from these somatic embryos exhibited normal plant regeneration on auxin-free medium.

The Electroresponse Properties of Alginate Films under the Electric Field (알지네이트 필름의 전기장 하에서의 응답 특성)

  • 김인중;강휘원;정창남
    • Polymer(Korea)
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    • v.27 no.3
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    • pp.195-200
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    • 2003
  • Alginate is a natural ionic polymer including numerous anionic groups and can be actuated by the ionic group under the electric field. The crosslinked alginate films were fabricated with CaCl$_2$. The thermal, mechanical and electroresponse properties of the films were investigated by thermogravimetric analysis, tensile and bending tests. The initial degradation and tensile strength increased according to the degree of crosslinking. Also, the swelling ratio of the films increased with decreasing degree of crosslinking and increasing pH due to free volume and electrostatic repulsion. The films actuated by an electric stimulus exhibited gentle and flexible action like a pendulum. In the electric field, the electric stimuli such as the applied voltage, ionic strength and kind of electrolyte solution had an effect on the electroresponse of the films. Alginate films with 5 wt% crosslinking agent showed the highest bending angle and reversible bending behavior. When the ionic strength of NaCl and KCl electrolyte solution was 0.1 M, the films showed the highest electroresponse. The bending behavior of the films increased with the applied voltage.

Dyeing Properties of Nylon 4 Copolymer fiber (Nylon 4 공중합 섬유의 염색성에 관한 연구)

  • Yoon, Seok-Han;Kwak, Dong-Sup;Cho, Hyun-Hok
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2012.03a
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    • pp.14-14
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    • 2012
  • Nylon 6 섬유는 우수한 기계적 물성과(강성, 탄성회복률 등) 염색성 등의 장점을 가지고 있어 의류용 등의 다양한 용도로 활용되어 왔다. Nylon 6 섬유를 포함한 대부분의 합성섬유는 천연섬유인 cotton 대비 흡수성이 낮고, cotton은 흡수성은 탁월하나 수분 건조속도가 매우 느리고 가격이 고가(약 10$/kg)여서 이를 대체할 새로운 고쾌적성 의류용 nylon 합성섬유의 개발이 시도되고 있다. 1953년 최초로 합성이 보고된 nylon 4는 구조상 소수성 탄소수가 적으며 친수성 아마이드기가 상대적으로 많아 흡수성과 속건성이 탁월한 이점을 가짐으로써 면을 대체할 수 있는 꿈의 합성섬유로 인식되었다. 1980년대 Chevron Research사를 중심으로 활발한 사업화 연구가 진행되었지만, 녹는점 $265^{\circ}C$) 대비 낮은 열분해온도($260^{\circ}C$) 특성으로 방사가공 시 내열성 문제를 극복하지 못해 아직까지 상업화에 이르지는 못하고 있다. 최근, 일본 산업기술종합연구소(AIST)를 중심으로 다시 바이오매스 유래 nylon 4의 개발이 시도됨에 따라 의류용 용도전개에 있어 가격 경쟁력을 갖춘 획기적인 소재 전환이 기대되고 있으며, 이로 인해 nylon 4 중합 연구가 다시 전 세계적 주목을 받기 시작하였다. 국내에서도 최근 nylon 4의 방사 내열성을 극복하고 기능성 부여가 가능한 nylon 4 공중합물에 대한 대체 연구가 본격적으로 진행 중에 있다. 본 연구에서는 현재 국내에서 진행 중인 nylon 4 공중합 섬유의 연구동향과 함께 nylon 6 대비 물성 및 염색특성 차이를 비교함으로써 향후 산업화에 기초 자료로 활용하고자 한다.

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Cloning of Acetyl CoA Carboxylase (fabE) in Escherichia coli (대장균의 acetyl CoA carboxylase유전자의 클로닝)

  • Park, Wan;Song, Bang-Ho;Hong, Soon-Duk
    • Microbiology and Biotechnology Letters
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    • v.14 no.2
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    • pp.181-186
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    • 1986
  • A defective lambda transducing phase carrying acetyl CoA carboxylase gene (fabE) from Escherichia coli chromosome (72 min on the current linkage map) has been isolated. A restriction map of the chromosomal region from defective transducing phage was established by digestion with combination of the restriction enzymes. No cleavage site for the enzyme EcoRI was found in this region. Restriction fragments were cloned from defective transducing phage into high copy number plasmid vector pACYC184 to generate hybrid plasmids which were capable of complementation of fabE temperature sensitive mutation. We show here that the fabE gene is located on a 3.4 megadalton Bam HI-SalI fragment with a HindIII site, which lies within the 7.4 megadalton BglIIfragment, by complementation analysis.

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Material Stability Assessment of Low Oxygen and Heating Treatment (저산소 및 열처리법에 대한 문화재 재질 안정성 평가)

  • Jang, Han Gyeol;Baek, Na Yeon;Kang, Dai Ill
    • Journal of Conservation Science
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    • v.30 no.2
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    • pp.149-156
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    • 2014
  • Low oxygen treatment and heating treatment are used to prevent insects in the field of food science. These eco-friendly control methods can be applied to biological control technique in conservation treatment of organic cultural properties. To evaluate material stability, low-oxygen treatment and low oxygen treatment are applied to wood, pigment, paper and textile that are related to historical wooden buildings. As a result, wood moisture content declined after low oxygen treatment. But decline rate is a little, so it can be expected to turn back original state as time passes. And test result on pigment, paper, textile of chrominance and strength of test materials are stable. But after heating treatment, pigments are separated.

Development of Assembly for Fast BOD Estimation and Its Applications (신속한 BOD 측정장치의 개발과 응용)

  • Soon, Moo-Jeong;Kim, Eun-Jeong;Jang, Seh-Yong;Hong, Daeil
    • Analytical Science and Technology
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    • v.7 no.3
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    • pp.285-291
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    • 1994
  • A microbial BOD sensor for the continuous estimation of BOD was been developed by immobilizing Trichosporon cutaneum, which was immobilized between a dialysis and a gas-permeable membrane, on an oxygen electrode. The optimum pH and temperature for BOD measurement using this sensor were pH 7.0 and $32{\sim}33^{\circ}C$, respectively. The best result was obtained at 2~3ml/min flow rate in 0.1M phosphate buffer solution. A linear relationship was observed between ${\Delta}DO$ and the concentration of standard GGA solution below 60mg/l(90ppm $BOD_5$). The reproducibility was found to be within 3% for the standard solution containing glucose 30mg/l and glutamic acid 30mg/l. The output DO value of this sensor was almost constant for 30 dalys. The response time and the recovery time were about 5 and 10 min, respectively. This sensor was employed for the BOD measurement of waste pollutants and was compared with $BOD_5$ method.

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A Literature Review on Cracked Teeth (균열치 증후군에 대한 문헌고찰)

  • Cho, Woong-Rae;Kim, Dae-Gon;Park, Chan-Jin;Cho, Lee-Ra
    • Journal of Dental Rehabilitation and Applied Science
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    • v.27 no.3
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    • pp.305-316
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    • 2011
  • Cracked teeth are a common problem with incomplete fracture that typically shows pain on biting and temperature stimuli. The most common cause of crack is known to be the masticatory accident. Predisposing factors were aging, old restoration, and iatrogenic stress concentration, etc. Accurate diagnosis is needed for detecting the extent of crack and pulp vitality. There are many diagnostic tools like bite test, direct observation with transillumination, dye penetration, and radiographs. Immediate treatment for pain relief and occlusal adjustment would be needed. Composite resin and bonded amalgam showed favorable prognosis. Moreover, cusp protection is reliable permanent treatment for cracked teeth. The purpose of this article is to review the literature about the prevention, diagnosis, and treatment for cracked teeth.

이온 에너지 분석을 통한 저손상 그래핀 클리닝 연구

  • Kim, Gi-Seok;Min, Gyeong-Seok;Yeom, Geun-Yeong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.218.2-218.2
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    • 2014
  • 그래핀은 높은 전기 전도도와 열전도도, 기계적 강도를 가지고 있고 동시에 높은 전자이동도($200,000cm^2{\cdot}V{\cdot}^1{\cdot}s{\cdot}^1$) 특성을 갖는 물질로써 차세대 소재로 각광받고 있다. 하지만 그래핀을 소자에 응용하기 위해서는 전사공정과 lithography 공정 과정에서 발생되는 PMMA(Poly methyl methacrylate) residue를 완벽하게 제거해야 하는 문제점이 있다. 특히, lithography 공정 중 완벽하게 PMMA residue 가 제거되지 않고 잔류해 있을 경우에 소자의 life time, performance에 악영향을 준다는 보고가 있다. 이와같은 문제를 해결하기 위해 화학적 cleaning, 열처리를 통한 cleaning, 전류 인가에 의한 cleaning과 같은 방법들을 이용하여 그래핀의 PMMA residue를 제거하는 공정들이 보고되고 있지만, 화학적 cleaning 방법의 경우 chloroform 이라는 독성물질 사용으로 인해 산업적으로 응용이 어렵고, 열처리 방법은 전극 등의 금속이 $200^{\circ}C$ 이상의 높은 온도에서 장시간 노출될 경우 쉽게 손상을 입으며, 전류 인가에 의한 cleaning 방법은 국부적으로만 효과를 볼 수 있기 때문에 lithography 공정 후 PMMA residue를 효과적으로 제거하기에는 한계를 보이고 있다. 본 연구에서는 Ar을 이용하는 Ion beam 시스템을 통해 beam energy를 제어함으로써 PMMA residue를 효과적으로 제거하는 연구를 진행하였다. 최적화된 플라즈마 발생 조건을 찾기 위해 QMS(Quadrupole Mass Spectrometer)를 이용하여 입사하는 ion energy와 flux 양을 컨트롤 하였고, 250 W에서 최적화된 ion energy distribution 영역이 존재한다는 것을 확인할 수 있었다. 또한, 25 Gauss 정도의 electro-magnetic field를 이용하여 Ar의 ion energy를 10 eV 이하로 낮추어 damage를 최소화함으로써 효과적으로 그래핀을 cleaning 할 수 있었다. Cleaning과정에서 ion bombardment에 의해 발생한 damage는 $250^{\circ}C$에서 6시간 동안 annealing 공정을 거치면서 회복되는 것을 Raman spectroscopy의 D peak ($1335cm{\cdot}^1$) / G peak ($1572cm{\cdot}^1$) ratio 로 확인할 수 있었고, PMMA residue의 cleaning 여부는 G peak ($1580cm{\cdot}^1$)의 blue shift와 2D peak ($2670cm{\cdot}^1$)의 red shift를 통해 확인하였다. 그리고 AFM (Atomic Force Microscopy)을 이용하여 cleaning 공정과정에서 RMS roughness가 4.99 nm에서 2.01 nm로 감소하는 것을 관찰하였다. 마지막으로, PMMA residue의 cleaning 정도를 정량적으로 분석하기 위해 XPS (X-ray Photoelectron Spectroscopy)를 이용하여 sp2 C-C bonding이 74.96%에서 87.66%로 증가함을 확인을 할 수 있었다.

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The Study of continuous cardiac output measurement module development of the cardiopulmonary function patient of using the Swan-Ganz Catheter (Swan-Ganz 카테터를 이용한 심폐기능 이상 환자의 지속적 심박출량 측정 모듈 개발)

  • Lim, Byeong-Seon;Han, Seung-Hwan;Kim, Young-Kil
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.4
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    • pp.959-964
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    • 2013
  • This study materialized the new module which enables to measure more precise data than the existing modules in order to examine the cardiac disorder critical patient's state by using Swan-Ganz Catheter. There was bolus type CO(Cardiac Output) module which measured CO by measuring the blood changes in temperature and recovery time by injecting cold sap into the heart in the past, but recently, it is not used in most of hospitals due to limit of difficulty of continuous monitoring for the patients. To overcome this limit, the continuous cardiac output measurement platform was materialized to enable the continuous monitoring for patients. The wasted cost issues because of introducing the expensive imported equipment to observe the critical patient`s state with abnormal cardiopulmonary function in the hospitals can be solved by using this new module, and the problem of existing modules should be supplemented for more accurate diagnosis by collecting more precise data.

Ecological Study on Aquatic Plant Communities in the Stream of Thermal Wastewater (온배수 유입하천에 형성된 수생식물군집의 생태학적 연구)

  • 길봉섭;유현경
    • Korean Journal of Environmental Biology
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    • v.17 no.2
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    • pp.139-146
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    • 1999
  • The seasonal changes of the flora at three samples sites such as Sockchong, Wang-gung and Chukrim hot spring by drained thermal waters were investigated from 1997 to 1998. Monthly occurrence of plant species in February and October showed more abundantly control site than that of heavy and/or light polluted sites. This trend demonstrated similarly in terms of seasonal changes of leaf area and plant growths, both fresh and dry weight, too. Cu, Zn, Ni and Pb content including plants from Sockchong hot spring site were increased from April to September at test area than that of control, while that was decreased in October. But Zn and Pb content holding plants from Wang-gung hot spring site represented higher test site than that of control. In short impacts on aquatic plants communities by thermal wastewater of sampled area have happened in the season of low temperature, and their restorations have done in summer season.

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