• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.211-216
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• 2009

Approximately 43 million tons of livestock manure (LM) are produced each year on Korean farms. LM can be utilized as a valuable resource and/or it can contaminate water by runoff and leaching through the soil, when LM has been thoughtlessly applied to the land and directly discharged into the water. This experiment was carried out to investigate the effect of no-till system and LM application on dry matter (DM) yield of silage corn and $NO_3$-N concentration in leaching water of lysimeter installed in the experimental field. The treatments were replicated three times in split plot design. Main plots consisted of tillage systems, such as conventional tillage (CT) and no-tillage (NT). Sub plots consisted of the type of LM, such as chemical fertilizer (CF), composted cattle manure (CCM) and composted swine manure (CSM). The control plots were fertilized as commercial chemical fertilizer. DM yields of corn increased significantly in order to CF > CCM > CSM (p<0.05). DM yield of corn in CT increased as comparing with that of corn in NT. Plant height, ear height and stem diameter also increased in order to CF > CCM > CSM. In addition, the root weight in CT was increased as comparing with that of corn in NT. However, there was no interaction effects of between type of LM and tillage system. $NO_3$-N concentration in leaching water of LM application was less than 10 ppm, but $NO_3$-N concentration in CF exceeded 10 ppm which is safety level of drinking water during summer time (rainfall season).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.4
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• pp.401-411
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• 2015

Drought stress has detrimental effects on the seedling development, vegetative/ reproductive growth, photosynthesis, root proliferation, anthesis, anthesis-silking interval (ASI), pollination and grain yield in maize. Typically, two weeks before silking through pollination are an important time in maize life. Here we reviewed the effects of drought stress on growth, physiological/ molecular researches for drought tolerance, and breeding to genomics in maize. Drought stress during kernel development increases leaf dying and lodging, decreases grain filling period and grain yield. Physiological factors of drought stress/ effects are water content, water deficits, and water potential. Nowdays molecular marker assisted breeding method is becoming increasingly useful in the improvement of new germplasm with drought stress tolerance.

• Korean Journal of Poultry Science
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• v.50 no.3
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• pp.171-185
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• 2023

This study was conducted to investigate the effects of supplementation of Allium hookeri (AH) root powder on the gut microbiome, immunity, and health in broiler chickens fed experimental diets from d 10 to 28. A total of 60 10-day-old Ross 308 broilers were weighed and assigned to two dietary treatments with 5 birds per cage in a randomized complete block design based on body weight. The two experimental diets consisted of a control diet based on corn-soybean meal and the control diet supplemented with 0.3% AH root powder. All birds were fed ad libitum with experimental diets and water for 18 d. At 28 d, two birds near the median weight from each cage were selected for cecal content and small intestinal tissue sample collection. The addition of AH changed the gut microbiome by increasing probiotic candidate beneficial bacteria such as Enterococcaceae, Lactobacillaceae, Limosilactobacillus, Cuneatibacter, and Ruminoccoides. Regarding gut immunity, the supplementation of AH resulted in changes in intestinal immune cells, including reduced CD3+CD4+ T cells, which are a type of helper T cell, in the small intestine of birds (P=0.049). Additionally, there was a tendency to increase the expression of antioxidant function-related gene such as GPX2 (P=0.060), but no significant changes were observed in cytokines such as IL1b, IL6, and IL10. Overall, the addition of AH root powder may have positive effects on the microbiome of the chickens. This may help promote gut health in broiler chickens at the age of d 10 to 28.

• Journal of Plant Biology
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• v.35 no.3
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• pp.237-245
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• 1992

To understand the properties of the cauliflower mosaic virus (CaMV) 35S promoter and the effect of the deleted maize alcohol dehydrogenase I-S (Adhl-S) intron 1 on the expression of the CaMV $35S{\beta}-glucuronidase$ (GUS) gene in potato (Solanum tuberosum L. cv. Superior), we constructed a chimeric gene and transferred it into potato with Agrobacterium tumefaciens mediated method. The pLS201, a gene transfer vector of 17.7 kilobase pairs, was composed of the CaMV 35S promoter, the 249 base pairs of deleted maize Adhl-S intron 1, the GUS reporter gene, and the kanamycin resistance gene as a selectable marker for transformation. The GUS activity was examined by histochemical and spectrophotometric assay in transformed potato plants. The GUS activity was found primarily around the vascular tissue cells in stem and root. In the spectorophotometric assay, the level of GUS activity of transgenic potato transformed with CaMV 35S/249 bp of intron 1 fragment-GUS (pLS201) was compared with that of potato transformed with CaMV 35S-GUS (pBI121). The quantitative spectrophotometric assay showed that the level of GUS activity in potato transformed with pLS201 was higher in leaf, stem and root by 30-, 34- and 42-fold, respectively than those in potato transformed with pBI121. This results indicate that the inclusion of the deleted maize Adhl-S intron 1 resulted in increament of the GUS gene expression in transgenic potato.potato.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.09a
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• pp.108-112
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• 2004

균열 암반 대수층에 대한 지하수 인공 함양 주입 가능성을 예비적으로 평가하기 위한 시험을 수행하였다. 지하수 인공 함양 주입 시험이 수행된 지역은 경기도 포천군 이동면이며, 이 지역의 지질은 중립내지 조립질 화강암에 해당된다. 시험 정호에 대한 시추공 내부 촬영 결과, 화강암 내에 부분적으로 절리들이 발달되어 있었으며, 대체로 수직적인 절리 발달이 우세하였다. 2개소에서 인공 함양 주입 시험이 100kgf/$\textrm{cm}^2$ 이상의 확장 팩커로 대상공의 상부를 밀폐한 후 시행되었다. 이중 MW-7호 공에서는, 주입 압력을 5 내지 7kg/$\textrm{cm}^2$으로 조절하여 시간당 약 450$\ell$의 평균 주입률로 시험을 수행하였으며, O-7a 호 공에서는 주입 압력을 4kgf/$\textrm{cm}^2$ 내외, 시간당 약 1,740$\ell$의 평균 주입률로 시험을 수행하였다. MW-7호 공의 시험은 3일간 3회에 걸쳐 각 450분, 200분, 414분 동안 시험이 수행되었으며, O-7a호 공에서는 연속적으로 24시간 동안 인공 함양 주입 시험이 수행되었다. 수행된 지하수 인공 함양 주입시험은 적어도 지하수 상류 구배 구간의 지하 수두를 시험이 수행된 이후에도 24시간 이상 유지하는 것으로 평가되었다. 실험을 통해 주입한 양과 주변 관측공의 수위 상승을 단순 검토한 결과 시험이 수행된 지역의 개략적 유효 공극률을 산정 할 수 있었으며, 그 결과, 이 지역 균열 암반의 유효 공극률은 약 3 내지 6% 인 것으로 평가되었다 국내에서 지하수 인공 함양 방식을 균열 암반 대수층에 활용하여, 지속적인 수자원 관리와 수도 공급을 할 수 있는지를 평가하기 위해서는 앞으로 보다 많은 시험수행과 연구를 통한 검증이 요구된다. 까마중, 냉이, 명아주, 둑새풀 등의 생장에 현저한 조해현상을 나타냈다. 이것으로 보아 억새가 타식물의 생장에 영향을 주는 요인물질은 억새의 뿌리에서 분필되는 것으로 생각된다. 옥수수의 뿌리에서 직접 분필하는 물질이나 옥수수뿌리의 분해물질들은 모두 당귀의 생장을 조해하는 경향이 있었다.기존에 제안된 경험식들에 의한 계산결과 보다 균질화 해석법의 결과가 훨씬 정확함을 주목하여야 한다.c의 범위로서 최대값과 최소값은 4차수(four order)의 차이를 보였다. 단열대의 분포 특성을 파악하기 위하여 지구물리검층을 실시하였고, 각 시험에 의해 획득된 결과들과의 비교를 통하여 유동성이 높은 단열들이 규명되었다. 온도검층은 유동성 단열과 일반적인 단열들을 구별하는 좋은 지시자로 나타났다. 그 결과, N70-80$^{\circ}$W.60-85$^{\circ}$NE/SW, N75-80$^{\circ}$W.25-30$^{\circ}$SW, N50-64$^{\circ}$W.60-85$^{\circ}$NE, N35-45$^{\circ}$E.65-75$^{\circ}$SE, 그리고 N65-72$^{\circ}$E.80$^{\circ}$SE/60$^{\circ}$NW의 단열들이 연구지역의 지하수 흐름을 지배하는 뚜렷한 유동성 단열로 규명되었다.eatments. It was resulted from increase of weight of single cocoon. "Manta"2.5ppm produced 22.2kg of cocoon. It is equal to 9% increase in index, as compared to that of c

• Research in Plant Disease
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• v.15 no.3
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• pp.242-247
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• 2009

To select rotation crops for control of clubroot of Chinese cabbage, potato, corn, soybean, onion and groundsel were planted in the field infected with clubroot pathogen (Plasmodiophora brassicae) in highland area in 2000. In comparison of economical efficiency among rotation crops, potato and onion gained about 16.9 and 14.9 times higher, respectively, than successive cultivation of Chinese cabbage. Resting spore densities of Plasmodiophora brassicae after harvesting rotation crops were in the range of $0.3{\sim}1.2{\times}10^3/g$ soil in all cultivated soils with rotation crops while that of successive Chinese cabbage cultivation soil was very high as much as $89.3{\times}10^3/g$ soil. And disease severity of Chinese cabbage clubroot was 4.9, 20.2, 24.4, 25.1 and 27.8% in onion, soybean, potato, corn, and groundsel cultivation plot, respectively, while that of successive Chinese cabbage cultivation plot was very high as 77.8%. Effect of rotation period of onion, potato, soybean on disease control was investigated from 2002 to 2005. Resting spore densities of Plasmodiophora brassicae after cultivating rotation crops were decreased until $2^{nd}$ year and maintained low density at $3^{rd}$ year in all plots, while that of successive Chinese cabbage cultivation plot was increased 2.6 to 23.6 times for three years. When Chinese cabbage was rotation-cultivated with potato, soybean and onion for three years, disease severities of Chinese cabbage clubroot decreased 92 to 4.4%, 72 to 10.4% and 72 to 12.2%, respectively, while that of successive Chinese cabbage cultivation plot maintained 100%. As the rotation period increased, the yields of Chinese cabbage increased, while that of successive Chinese cabbage cultivation plot decreased. At $3^{rd}$ year, Chinese cabbage with high quality could be much more produced 2,205, 2,493 and 2,476 g in potato, soybean and onion cultivation plot, respectively, than 95 g in successive Chinese cabbage cultivation plot.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.4
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• pp.422-431
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• 2019

Severe droughts in spring have occurred frequently in Korea in recent years, exerting a critical impact on corn yield. Therefore, it is necessary to find physiological and/or molecular indicators of the response to drought stress in maize plants. In this study, we investigated the effects of water-deficit stress on two Korean elite F₁ maize hybrids, Ilmichal and Gwangpyeongok, by withholding water for 10 days at tassel initiation. The water deficit drastically reduced the relative leaf water content, leaf number, leaf area, and stem length, leading to dry matter reduction. Moreover, it reduced the SPAD values and stomatal conductance of leaves in drought-stressed plants of both hybrids. Importantly, the number of leaves and SPAD value were non-destructive and easy to investigate in response to water-deficit stress, suggesting that they may be useful indicators for screening drought-tolerant genetic resources. We detected more than 100 spots that were differentially accumulated under drought stress. Of these spots, a total of 21 protein spots (≥1.5-fold) from drought-exposed maize leaves were successfully analyzed by MALDI-TOF-TOF mass spectrometry. Functional annotation using Gene Ontology analysis revealed that most of the identified proteins were involved in carbohydrate metabolism, stress response fatty acid catabolism, photosynthesis, energy metabolism, and transport. The protein expression levels were increased in both Ilmichal and Gwangpyeongok, except for triosephosphate isomerase, fructose-bisphosphate aldolase, and an uncharacterized protein. The lactoylglutathione lyase delta (3,5)-delta (2,4)-dienoyl-CoA isomerase was overexpressed in Gwangpyeongok only. The results obtained from this study suggest that the drought-specific genes may be useful as molecular markers for screening drought-tolerant maize genotypes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.4 no.1
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• pp.1-23
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• 1968

This study aimed at contributing to the improvement of cropping systems after finding out the effects of excrements and components of crop root influence on other crops as well as themselves. The following forage crops suitable for our country were selected for the present study. Aqueous extracts of fresh roots, aqueous extracts of rotting roots and aqueous solutions of excrements of red clover, orchard grass and brome grass were studied for the effects influencing the germination and growth of seedlings of red clover, ladino clover, lespedeza, soybean, orchard grass, Italian ryegrass, brome grass, barley, wheat, sorghum, corn and Hog-millet. In view of the possibility that the organic acid might be closely related to the excrements and components of crop root connected with soil sickness, the acid components of three species of roots were analysed by paper chromatography and gas chromatography method. The following results were obtained: 1. Effects of Aqueous Extracts of Fresh Roots : Aqueous extracts of red clover: The extracts inhibited the growth of seedlings of the ladino clover and lespedeza and also inhibited the development of most crops except that of sorghum among the Graminaceae. Aqueous extracts of orchard grass: The extracts promoted the seedlings growth of red clover and soybean, while it inhibited the germination and growth of orchard grass. There were no noticeable effects influencing other crops while it inhibited the growth of barley and Hog-millet. Aqueous extracts of brome grass: There was no effect on Italian ryegrass but there was an inhibiting effect on the other crops. 2. Effects of Aqueous Extracts of Rotting Roots : Aqueous extracts of red clover: The extracts promoted the seedling growth of red clover. But it reflected the inhibiting effects on other crops except sorghum. Aqueous extracts of orchard grass: The extracts promoted the growth of red clover, ladino clover, soybean and sorghun, while it inhibited the germination and rooting of barley and Hog-millet. Aqueous extracts of brome grass: The extracts gave the promotive effects to the growth of red clover, soybean and sorghum, but caused inhibiting effects on orchard grass, brome grass, barley and Hog-millet. 3. Effects of Aqueous Solutions of Excrements : The aqueous solution of excrements of red clover reflected the inhibition effects to the growth of Graminaceae, while the aqueous solutions of excrements of orchard grass and Italian ryegrass caused the promotive effects on the growth of red clover. 4. Results of Organic Acid Analysis : The oxalic acid, citric acid, tartaric acid, malonic acid, malic acid and succinic acid were included in the roots of red clover as unvolatile organic acid, and in the orchard grass and brome grass there were included the oxalic acid, citric acid, tartaric acid and malic acid. And formic acid was confirmed in the red clover, orchard grass and brome grass as volatile organic acid. In consideration of the results mentioned in above the effects of excrements and components of roots found in this studies may be summarized as follows. 1) The red clover generally gave a disadvantageous effect on the Graminaceae. Such trend was considered chiefly caused by the presence of many organic acids, namely oxalic, citric, tartaric, malonic, malic, succinic and formic acid. 2) The orchard grass generally gave an advantageous effect on the Leguminosae. This may be due to a few kinds of organic acid contained in the root, namely oxalic, citric, tartaric, malic and formic acid. Furthermore a certain of promotive materials for growth was noted. 3) As long as the root of brome grass are not rotten, it gave a disadvantageous effect on the Leguminosae and Graminaceae. This may be due to the fact that several unidentified volatile organic acid were also included besides the confirmed organic acid, namely oxalic, citric, tartaric, malic and formic acid. 5. Effects of Components in Roots to the Soil Sickness : 1) It was considered that the cause of alleged red clover's soil sickness did not result from the toxic components of the roots. 2) It was recognized that the toxic components of roots might be the cause of soil sickness in case the orchard grass and brome grass were put into the long-term single cropping. 6. Effects of Rooted Components to the Companion Crops in the Cropping System : a) In case of aqueous extracts of fresh roots and aqueous excrements (Inter cropping and mixed cropping) : 1) Advantageous combinations : Orchard grass->Red clover, Soybean, Italian ryegrass->Red clover, 2) Disadvantageous combinations : Red clover->Ladino clover, Lespedeza, Orchard grass, Italian ryegrass, Fescue Ky-31, Brome grass, Barley, Wheat, Corn and Hog.millet, Orchard grass->Lespedeza, Orchard grass, Barley and Hog-millet, Brome grass->Red clover, Ladino clover, Lespedeza, Soybean, Orchard grass, Brome grass, Barley, Wheat, Sorghum, Corn and Hog-millet, 3) Harmless combinations : Red clover->Red clover, Soybean and Sorghum, Orchard grass->Ladino clover, Italian ryegrass, Brome grass, Wheat, Sorghum and Corn, Brome grass->Italian ryegrass, b) In case of aquecus extracts of rotting roots(After cropping) : 1) Advantageous combinations : Red clover->Red clover and Sorghum, Orchard grass->Red clover, Ladino clover, Soybean, Sorghum, and Corn, Brome grass->Red clover, Soybean and Sorghum, 2) Disadvantageous combinations : Red clover->Lespedeza, Orchard grass, Italian ryegrass, Brome grass, Barley, Wheat, and Hog-millet Orchard grass->Barley and Hog-millet, Brome grass->Orchard grass, Brome grass, Barley and Hog-millet, 3) Harmless combinations : Red clover->Ladino clover, Soybean and Corn, Orchard grass->Lespedeza, Orchard grass, Italian ryegrass, Brome grass and Wheat Brome gass->Ladino clover, Lespedeza, Italian ryegrass and Wheat.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.3
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• pp.197-204
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• 1989

To evaluate the effect of soil improvement on the chemical properties and corn silage yield, this experiment was carried out in the newly-reclaimed sloped land. Corn (Suwon 19) was cultivated under the six different treatments including integrated improvement plot at Songjeong loam, 20 percent slope, from 1985 to 1987 and various soil chemical properties and silage yield were investigated. pH of topsoil was above 5.0 except for control and phosphate plot, but lime and integrated improvement plots were above 5.0 in subsoil. The contents of organic matter of topsoil were above 2.0% except control and subsoiling plot. Except control, the range of cation exchange capacity was 7.4-7.8 me/100g in topsoil, 7.0-7.7 me/100g in subsoil. Soil bacteria density of root zone was the highest in integrated improvement than the other treatments, and it was higher at the harvesting stage than the heading stage. Mean density of microorganism was $61.3{\times}10^5$ in bacteria, $21.5{\times}10^4$ in fungi and actinomycetes was B/F ratio was 28.5 and B/A ratio was 2.9. Vertical root distribution of total and 10cm depth below was more in the integrated improvement and subsoi ling plot than the other plots. Total nitrogen (T-N) content was higher in integrated improvement plot, and phosphate content of leaves was higher in compost and integrated improvement plot, but stem and grain were not different. Potassium content of the plant was higher in integra ted improvement plot. Correlation between dry matter yield and T-N was more significant than the other elements. And the contents of phosphate, calcium and magnesium were significant at 5%, but potassium was not.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.4
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• pp.284-291
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• 2001

To study whether N isotope composition (${\delta}^{15}N$) of crop reflects the kind of fertilizer (chemical or organic) applied to field, a pot experiment was conducted. Corn (Zea mays L.) was cultivated under greenhouse conditions for 70 days. Composted pig manure and urea were applied at 0 and 0 (C0U0), at 0 and 300 (COU2), at 300 and 0 (C2U0) and at 150 and $150kg\;N\;ha^{-1}$ (C1U1), respectively. The ${\delta}^{15}N$ values of composted pig manure and urea were + 13.9‰ and -2.3‰, respectively. The ${\delta}^{15}N$ values of whole parts (roots + stems + leaves + grains) were + 12.7, + 12.9, + 14.0 and + 13.0‰ for C0U0, C0U2, C2U0 and C1U1 treatments, and were not significantly affected by the application of isotopically different N sources (P<0.05). However, leaves or grains showed significantly (P<0.05) different ${\delta}^{15}N$ values between treatments. The ${\delta}^{15}N$ values of leaves and grains were + 14.3 and + 16.2‰ for C2U0, +13.2 and +13.9‰ for C0U0, +10.1 and + 12.6‰ for C1U1 and +10.1 and +12.4‰ for C0U2 treatments. The different ${\delta}^{15}N$ values of corn from the values of N sources (compost and urea) applied to soil showed that the ${\delta}^{15}N$ values of corn were affected not only by the isotope composition of N source, but also by N pool mixing and isotope fractionation accompanying N transformation. This study suggests that although the ${\delta}^{15}N$ values of crop are not identical to the ${\delta}^{15}N$ values of N sources applied to fields, the application of isotopically different N sources such as compost and chemical fertilizer may result in qualitative difference in ${\delta}^{15}N$ values of crop.