• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.26 no.1
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• pp.20-25
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• 1990

During the summer of 1989, the authors carried out the hydroacoustic surverys to investigate the vertical distribution of volume backscattering strength in the East China Sea and simultaneously the biological sampling of the scattering layers by bottom trawling. The echoes from the scattering layers was continuously measured by using a 50 kHz echo sounder during the day and night. A data acquisition system was used to record digitally the envelope of the echoes and the echo integration technique was used to determine the scattering strength proportional to biomass density in each layer. The vertical profiles of volume backscattering strength also were compared with the one of water temperature. The results obtained can be summarized as follows: 1. The vertical profiles of mean volume backscattering strength at day and night suggested that during the night the biggest fish concentrations appeared in the mixed layer above the thermocline and during the day near the bottom. In another profiles where the thermocline was not well developed, peaks in scattering appeared at midwater depths and near the bottom. 2. The maximum values of mean volume backscattering strengths varied from -49.3 dB to -48.0 dB on different regions and at different times of the day and night. 3. Trawl data indicated that the organisms consisting of the scattering layer near the bottom were squid and various species of demersal fishes.

• Korean Journal of Food Science and Technology
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• v.26 no.3
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• pp.261-265
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• 1994

To detect naturally occurring bioactive compounds in unused marine resources such as marine by-products and algae, the screening test for the antimicrobial compounds containing in Asterina pectinifera, Halocynthia rotetzi skin, Nototodarus sloani ink. Anthocidaris crassispina skin, and four algae(Sargassum horneri, Agarum cribrosum, Odonthalia corymbifera and Desmarestia ligulata) was carried out. The minimum inhibitory concentrations(MIC, ${\mu}g/disk$) of ether and methanol fractions extracted from Sargassum horneri against Bacillus subtilis were 80 and 70 ${\mu}g/disk$, respectively. The MICs of the ether, acetone and methanol fractions extracted from Sargassum horneri against Staphylococcus aureus were 160, 180 and 70 ${\mu}g/disk$, respectively. In addition, the MICs of ether and acetone fractions extracted from Odonthalia corymbifera against Staphylococcus aureus were 50 and 170 ${\mu}g/disk$, respectively. These nonpolar fractions showed the strongest antimicrobial effect among several fractions extracted from tested marine resources.

• Analytical Science and Technology
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• v.26 no.5
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• pp.307-314
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• 2013

Selenium exists in various forms of chemical species. The activity and bioavailability is strongly dependent on its chemical form and concentration. Consequently the information on each selenium species and its concentration must be exactly determined for the food we take in. In this study, selenium species in seafood were separated and quantified by RP (reversed phase) HPLC (high performance liquid chromatography) coupled with ICP-MS (inductively coupled plasma mass spectrometry) using post-column isotope dilution. $^{79}Br$, which interferes on $^{80}Se$, has mostly been removed by solid phase extraction and then mathematical correction has been applied for the more accurate correction. The experimental result for CRM (certified reference material) DOLT-4 agreed well with the certified value but each selenium species could not be compared. SeCys (selenocysteine) and SeMet (selenomethionine) were the major species detected in seafood such as belt fish, spanish mackerel, and squid that have been serving as Korean diet. The concentrations found in Korean sea food for SeCys and SeMet were in the range of 0-661.6 mg/kg and 137.3-462.7 mg/kg, respectively.

• Food Science of Animal Resources
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• v.23 no.2
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• pp.115-121
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• 2003

The pork quality was determined with following treatments. The meat samples were obtained from pigs which had been fed finishing pig diets containing 5% beef tallow(Control), 3% beef tallow and 2% perillar seed oil(T1), 250 ppm vitamin E(a-tocopheryl acetate) in T1(T2), 3% beef tallow and 2% squid viscera oil(T3), 250 ppm vitamin E in T3(T4), 3% beef tallow and 2% CLA(Conjugated linoleic acid, T5). T1 had the lowest sarcomere length, salt solubility and total protein contents among the treatments. Salt solubility and total protein content of T2 and T4 which had been fed diets containing Vit. E were higher than those of T1 and T3 which had not been fed diets without Vit. E. pH and water holding capacity(WHC) values of control were higher than those of T1, T3 and T5, while WHC of T2 and T4 was higher than those of T1, T3 and T5. The hunter L value of meat and a value of fat showed higher in T5 than those in control, T, T3. The adhesiveness of T3 and the springiness of T5 in cooked meat showed higher level than other treatments.

• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.519-524
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• 2000

This study has been carried out to estimate the amount of the daily intake on artificial sweetener(aspartame and sodium saccharin) and antioxidants(propyl gallate, BHA, BHT and TBHQ) in food consumed by Korean. The mean daily intake for each food item were obtained from the report of National Nutrition Survey carried by Ministry of Health and Welfare in 1997. The contents of artificial sweetener and antioxidants have been analyzed by HPLC. Aspartame was detected on 22 of 239 samples in the range of $33.4{\sim}3308.0\;mg/kg$, sodium saccharin was detected on 6 of 115 samples in the range of $8.3{\sim}41.8\;mg/kg$. BHA(204.9 mg/kg) was detected in Chewing gum only. Total estimated daily intake(EDI) of each additives per capita per day are as follows; 2.336 mg/person/day for aspartame, 0.259 mg/person/day for sodium saccharin and 0.002 mg/person/day for BHT. These values were less than 1% of FAO/WHO's acceptable daily intake(ADI).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.5
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• pp.367-375
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• 2006

The chemical components, lipid class, and fatty acid composition of muscle from male and female common squid, Todarodes pacificus, were examined to evaluate the potential utilization of muscle from fin, head, arms, and tentacles, which consumers usually like less than the mantle. The mantle was found to constitute 47-49% of the total muscle and the proportion was slightly higher in females than in males. For the remaining 51-53% of the muscle, the only gender difference was that the arms of males contained approximately 3% more muscle than those of females (P<0.05). The protein content was higher in the mantle, arms, and tentacles than in the fin and head in both males and females (P<0.05), and was slightly higher in males (15.7-20.7%) than in females (15.1-19.2%). By contrast, the lipid content was slightly higher in females (1.82-2.54%) than in males (1.01-2.37%), and the fins in both males and females contained the most lipids (2.37-2.54%) of all muscle. The prominent lipid classes in the muscles were free sterol (males 81.5-91.9% vs. females 84.9-91.8% for the non-polar lipid content), phosphatidylcholine (PC, males 59.3-62.4% vs. females 49.2-57.8% for the phospholipid content) and phosphatidylethanolamine (PE, males 22.0-28.8% vs. females 25.6-33.8% for the phospholipid content). The percentage of PC was approximately 5-10% higher in males (P<0.05), especially in the fin, while that of PE was approximately 3-5% higher in female (P<0.05), especially in the head. All of the squid muscle contained 52.1-54.9% of n-3 polyunsaturated fatty acids (PUFA), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). Males contained slightly more DHA, whereas female contained more EPA. The total percentage of n-3 PUFA differed little among muscles within the same gender.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.5
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• pp.376-383
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• 2006

The chemical components, lipid class, and fatty acid composition of the viscera from male and female common squid, Todarodes pacificus, were examined to evaluate the possible utilization of the liver, reproductive organs, and gills. In male and female squid, the viscera comprise 21% and 27% of the body weight, respectively. The protein content of the viscera was slightly higher in females (17.7-19.5%) than in males (15.6-17.2%). This was especially marked in the female reproductive organs, while there was little difference in the gill. The liver contained the largest amounts of lipids (17.2-18.6%) and the levels were higher in males than in females (P<0.01). By contrast, the reproductive organs of females contained more lipids than did those of males (4.68% vs. 1.65%, p<0.01). The prominent non-polar lipid (NL) classes were triacylglycerol (51.9-55.4% of the NL content) and sterol ester (16.3-21.8%) in the liver, and free sterol (47.0-68.5%) and free fatty acids (31.5-41.2%) in the reproductive organs. However, there were no significant differences in the NL classes between sexes. The percentage of the most prominent phospholipid (PL) class, phosphatidylcholine (PC), was highest in the liver (78.1-79.6% of the PL content), and there was no significant difference between the sexes. By contrast, phosphatidylethanolamine (PE) was highest in the reproductive organs (33.4%), and was higher in males than in females (P<0.05). All the visceral organs contained 36.4-48.5% of n-3 polyunsaturated fatty acids (PUFA), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). The DHA level was highest in female reproductive organs (32.3%), while EPA was high in male reproductive organs. These results demonstrate that the viscera of male and female common squid are a good source of DHA and EPA.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.745-750
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• 2014

This study investigated the effects of squid Todarodes pacificus liver oil on the serum lipids and adipose tissue weight of rats. The rats in the soybean oil (SO) group received a basic diet prepared according to the recommendations of the American Institute of Nutrition (AIN-93G), with 7% SO as lipid source. The rats in the Patagonian toothfish Dissostichus eleginoides muscle oil (PTFO) group received 5% PTFO and 2% SO as the lipid source, while the rats in the eco-friendly squid liver oil (EFSO) group received 5% EFSO and 2% SO. EFSO was prepared according to the method outlined in the author's patent. PTFO was extracted from muscle using a physical method in the author's laboratory. The prominent polyunsaturated fatty acids in the dietary oils were 18:2n-6 (54.3%) and 18:3n-3 (5.64%) in SO, DHA (6.77%) and EPA (3.61%) in PTFO, and DHA (26.2%) and EPA (9.94%) in EFSO. After feeding for 4 weeks, the serum lipid levels (e.g., triglyceride, total cholesterol, and low-density lipoprotein cholesterol) of the rats in the EFSO group were significantly reduced as compared with those in the SO group (P<0.05); those of the rats in the PTFO group were also reduced, albeit not significantly, compared with the SO group. On the other hand, the total white adipose tissue (visceral, epididymal, and perirenal) weight was significantly reduced in the EFSO and PTFO groups compared to the SO group. These results demonstrate that EFSO is an effective lipid modifying agent in rat blood, and that EFSO and PTFO have greater anti-obesity effects than SO.

• Journal of Aquaculture
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• v.10 no.2
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• pp.143-151
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• 1997

A six week feeding trial was conducted to determine the amount of fish meal analog (FMA) that can be replacing fish meal protein (FM) in Korean Rockfish. Seven experimental diets were formulated on isonitrogenous 52% crude protein and isocaloric basic 16.8KJ/g diet. Also, foreign commercial fish meal analog (CFMA) and attractants (ATT) were tested in this experiment. Percentage of the graded level of replacement of FM by FMA/CFMA on the basis of crude protein were as following : Diet 1, 100%FM ; Diet by 2, 60%FM : 40% CFMA ; Diet 3, 60%FM : 40$ CFMA+ATT ; Diet 4, 80%FM : 20%FMA ; Diet 5, 80%FM : 20% FMA+ATT ; Diet 6, 60%FM : 40%FMA+ATT ; Diet 7, 40%FM : 60% FMA+ATT. The FMA was made by mixing six animal protein source such as th blood meal, squid liver powder, meat and bond meal, leather meal, feather meal, poultry by-product and 3 essential amino acids (Met, Lys, Ile). Weight gain, feed efficiency, specific growth rate and protein efficiency ratio of fish fed diets 4, 5 and 6 were not significantly different (P>0.05) from those of fish fed the control (100% FM), while those of fish fed diets 2 and 3 were significantly lower (P<0.05) than those of fish fed the diet 6. There was no significant ATT effects in this study (P>0.05). Significant differences were found in hepatosomatic index, hemoglobin and condition factor. Therefore, these, results indicated that FMA can be used up to 40% as a substitute of fish meal protein in Korean Rockfish diets.

• Journal of Aquaculture
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• v.11 no.4
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• pp.487-493
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• 1998

This study was conducted to investgate the effect of fish meal analogue (FMA) on the growth of Israeli carp, Cyprinus carpio averageing 43g. Leather meal, meat and bone meal, feather meal, squid liver powder, poultry by-product and blood meal were used for ingredient of FMA. Four experimantal diets were formulated to contain 36% crude protein and 15.4 kJ available enery g-1. The percentage of follows ; Diet 1, 100% 3, 60% WFM + 40% FMA (40% FMA) ; Diet 4, 40% WFM + 60% FMA (60% FMA). In this expriment, fingerling Israeli carp averaging 43 g were assigned to each diet treatment. When the experiment was terminated, weigth gain (WG), feed conversion (FC), specific growth rate (SGR) and protein efficiency ratio (PER) were measured respectively. In the experiment, WG, FC, SGR and PER of Diet 2 were not significantly different from those of the control diet (P0.05). And WG, FC and SGR of Diet 3 were not significantly of the control Diet. In Diet 4, WG, FC and PER were not significantly different from those of the control Diet, but SGR was lower than that fo the control Diet. According to the results from this study, it might be possible to replace fish meal protein by FMA up to 60% in fingerling Israeli carp diets.