• Korean Journal of Plant Taxonomy
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• v.42 no.4
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• pp.316-323
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• 2012

Morphological characters of Adonis pseudoamurensis W. T. Wang and its related species were examined to illustrate its taxonomic entity, and their new key was made. As a result, it shows that A. ramosa Franch., an endemic species in Japan, was sparsely pubescent on the abaxial surface of the leaf and petiole, and its sepal was as long as its petal. However, A. pseudoamurensis is glabrous on the same surfaces and its sepal was shorter than its petal. In addition, A. multiflora Nishikawa et Ko. Ito was clearly distinguished in that it had acuminate leaf apices, linear shape of the final lobes of leaf and scales on the branched stem. Meanwhile, A. pseudoamurensis featured petiolate at cauline leaves and pubescent on pistils, and these characters were common with their allied groups. But diagnostic key characters suggested by the original description such as sessile at cauline leaves and glabrous on pistils were not observed in this study.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.479-484
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• 2000

This study was conducted to examine the effects of explant sources and plant growth regulators on mass propagation of Cyclamen persicum. Tuber, cotyledon, and petiole tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Shoots were not induced from calli on cotyledon and petiole explants cultured on MS medium containing various concentrations of 2,4-D or NAA. However, multiple shoots were formed directly from tuber explants cultured on the medium containing 0.5 and 1.0 mg/L 2,4-D or NAA. In MS medium with cytokinin alone, TDZ was more effective in shoot formation than BA or kinetin in all explants. The combinations of NAA and BA was found to be most effective in shoot formation from tuber, cotyledon and petiole explants. Especially, shoots were formed from all the tuber explants on the medium containing 0.5 mg/L of NAA and BA. Hormonal effects on root formation were examined by subculturing single shoots on MS medium containing NAA or IBA. The medium with 0.5 mg/L IBA was most effective in root induction and subsequent plantlet regeneration.

• Korean Journal of Environmental Agriculture
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• v.15 no.3
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• pp.383-390
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• 1996

This experiment was conducted to investigate the influence of nitrogen fertilizer forms, fertilizer and herbicide application rates on growth of radish. The nitrate content and nitrate reductase activity of radish were analyzed along with the growing stage. Nitrate nitrogen was more efficient than ammonium nitrogen for radish. With increasing the fertilizer application rate, accumulated of nitrate content was increased. The amount of nitrate nitrogen was highest at 25days after seeding in petioles, and $32{\sim}39days$ after seeding in root. Nitrate content was decreased as sampling date was delayed, whereas the content increased in the root at early growing stage and then decreased. The nitrate cotent increased in the order of petioles, roots, and leaf blades and nitrate reductase activity increased petioles, leaf blades, and roots. The higher nitrogen fertilizer and herbicide application increased nitrate nitrogen accumulation in radish as compaired with control treatment and nitrate reductase activity showed similar trend.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.279-284
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• 2008

This study carried out to investigate the variation of flavonoids contents in mungbean (Vigna radiata L.) according to plant parts, harvesting time, growth stage, and sowing time. Vitexin and isovitexin were found only in the seeds but not in the leaves, petioles, stems, and roots. Vitexin and isovitexin in seeds were detected only in the seed coat at concentration of 51.1 and $51.7\;mg{\cdot}g^{-1}$, respectively, but not in the cotyledon. There were no differences in the content of vitexin and isovitexin in mungbean seeds according to early, recommended and late harvesting times. Rutin in leaves was isolated and identified as a functional substance. The content of rutin was the highest in the leaves and higher in the order of petioles and stems. However, there was no rutin in the seeds, roots, and pods. The highest rutin content in the leaves, petioles and stems was observed at the 3rd leaf stage, which was higher in the order of the 5th and 7th leaf stage.

• Korean Journal of Medicinal Crop Science
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• v.7 no.1
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• pp.7-10
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• 1999

The effects of storage temperature and periods as pretreatments on direct somatic embryogenesis or shoot development in liquid culture of leaves, stems and petioles of Rehmannia glutinosa and Scrophularia buergeriana were investigated. Proper storage Temperature for Rehmannia glutinosa was $8^{\circ}C$ and the leaf stock could be stored up to 12 weeks. In Scrophularia buergerjana. shoots were developed from the 20 week-stored petiole and stem at $8^{\circ}C$. In general, $8^{\circ}C$ was considered the most effective storage temperature in terms of storage cost.

• Korean Journal of Plant Resources
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• v.14 no.3
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• pp.229-234
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• 2001

In order to investigate the possibility of utilizing small cermet as a seed corm in taro, the small cermets classified by the weight were planted on April 30,2000. The effect of weight of small cermets(5,10, If,20,25, and 30g) and corms on the growth, yield, and quality of taro was compared to those raised from cermets. The larger the size of cormel was, the more number of leaves developed, and the higher the yield was. Also the growth of small cormel was normal, so we forecast that it is possible to use small cermet as seed corm in mass production of taro.

• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.52-63
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• 1984

This study was intended to identify 17 taxa. (5 varieties and 12 species) of the genus Acer in Korea on the basis of the shapes of stomata, the type of trichome on the different part of leaves, the shapes, arrangement and number of stele in cross section of petiole. The results obtained were summarized as follows; 11 The shape of guard cells of stomata in the genus Acer was anomcytical, and the size of the cells ranged from 10.25 to $21.00{\mu}$ in length and from 7.57 to $11.83{\mu}$ in width. 2) Eleven types of trichome on the leaf in the genus Acer were found; pilose, sericeous, velutinous, woolly, glabrate, puberulent, bladder hair, hispid, hirsute and uncinate. This characteristics also established a good criterion for identification of species. 3) The stele of petiole in the genus Acer was characterized by eustele and atactostele with polybranch, and the six groups of the shape of numerical change of stele; B>M=T, BM

• Korean Journal of Plant Resources
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• v.20 no.1
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• pp.7-11
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• 2007

This study was conducted by estimate of leaf characteristics and growth performance among selected 20 families progenies of Hovenia dulcis var. koreana Nakai. Mean seedling height and root-collar diameter in whole progenies showed 61.6cm, 6.98mm, respectively. Mean seedling height of KW 2-5 family was 79.3cm and it was higher than that of other families. JN 2-2 family had highest value, 9.16mm, in mean of root-collar diameter. Growth performances of KW 2-5 and JN 2-2 families showed 57% (in seedling height), 53% (in root-collar diameter) superior to those of lowest marked families. The selection level based on growth performances, which are seedling height over 75.0cm and root-collar diameter over 8.0mm, were applied on whole families, and two families selected (KW 2-5, JN 2-2). The selection effects from selected families was evaluated as 125% compared to the mean of whole families. The coefficient of variation (C.V) appeared wide range, $9.4{\sim}42.4%$, in estimated leaf characteristics. Particularly, C.V of petiole length (PL) and petiole diameter (PD) showed 42.4% and 28.7%, respectively. Their were higher value than other leaf characteristics. Analysis of variance for all leaf characteristics were significantly different among families. Also, there had positive correlation between most leaf characteristics except the correlation between leaf length (LL) and leaf morphological index (LMI, LW/LL). Particularly, the correlation between leaf length and leaf width (LW) showed the highest correlation.

• Journal of Bio-Environment Control
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• v.7 no.3
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• pp.206-213
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• 1998

This experiment was conducted to investigate the effects of concentration of NO$_{3}$$^{[-10]}$ , $K^{＋}$ and $Ca^{＋＋}$ in nutrient solution on the seedlings growth of ‘Mudeungsan’watermelon. Seeds were sown on March 16, 1997. NO$_{3}$$^{[-10]}$ , $K^{＋}$ and $Ca^{＋＋}$ was treated with three different levels, respectively, NO$_{3}$$^{[-10]}$ = 106, 206, 406ppm; $K^{＋}$ = 150, 200, 400 ppm; $Ca^{＋＋}$ = 150, 200, 400 ppm. Plant growth was investigated at four-days interval in 10 days after treatment. Increasing N concentration in the nutrient solution increased the plant height, leaf area, number of leaves, fresh and dry weight of shoot while high concentration of K and Ca in the nutrient solution decreased the seedling growth in terms of leaf area, leaf dry weight. The uptake of N, K and Mg in petiole sap was positively affected by the adding of 206 ppm of nitrate, 150 ppm of potassium and 200 ppm of calcium levels, respectively. The P and Ca uptake in petiole sap was not affected by the N and K concentrations in the nutrient solution while K concentration above 200 ppm decreased the N and Mg content in petiole sap. High concentration of Ca in the nutrient solution increased the Ca content in petiole sap while Ca concentration above 200 ppm decreased the N content.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.483-488
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• 2011

Cacalia firma recently has been used increasingly as leaf vegetables but endangered in natural forest. In this work, we established the plant regeneration via adventitious shoot formation from petiole segments of seedling and in vitro plantlets. Wounding of seed coats and $GA_3$ treatments were effective to induce in vitro germination of seeds, whereas, seed did not germinate at all without these treatment. When cotyledon, leaf, petiole, and root segments of seedling were cultured on medium with 2 $mg{\cdot}L^{-1}$ benzyl adenine (BA) and 0.5 $mg{\cdot}L^{-1}$ naphthaleneacetic acid (NAA), petiole segments showed highest number of shoots per explant among the other segments. Among the various kinds of cytokinins, BA, isopentyl adenine (2-ip), kinetin, zeatin, thidiazuron (TDZ), TDZ and BA treatments were effective to induce high frequency of adventitious shoot formation from petiole segments of in vitro propagated plants. NAA stimulated the frequency of adventitious shoot formation but not for number of adventitious shoots per explants compared to TDZ or BA treatment alone. Most of adventitious shoots were developed directly from surfaces of explants. Adventitious shoots were transferred on medium with IBA for root formation, thereafter the plantlets were successfully transferred to soil.