• Korean Journal of Ecology and Environment
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• v.33 no.4 s.92
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• pp.366-373
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• 2000

Toxin production and low digestibility of cyanobacteria are known to cause low exploitability of cyanobacteria by zooplankton. In this study, we compared relative tolerance and compatibility of zooplankton taxa in eight eutrophic lakes, exposed to frequent cyanobacterial blooms, uring the summer season of 1999. Microcystis, Anabaena, Oscillatoria and Phormidium were common cyanobacteria in all lakes. with relatively lower $NO_3-N$ concentration (<0.2 mgN/l) and TN/TP ratio (<20), compared with other lakes where colonial cyanobacteria dominated. Rotifers were dominant zooplankton in most lakes, and among them, Keratella, Polyarthra and Hexathra were common. The laboratory feeding experiment showed that relative copepods that greatly decreased (90%) after 4 day when cyanobacteria were used as the food source of zooplankton, while rotifers gradually increased with the change of dominant taxa from Keratella through Pompholyx to Monostyla. These results suggest that rotifers may be capable of coexisting with cyanobacteria by exploiting them for the food source.

• Korean Journal of Soil Science and Fertilizer
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• v.12 no.1
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• pp.49-53
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• 1979

Effects of temperature and light intensity on photosynthesis, respiration and chlorophyll content of ginseng (Panax ginseng C. A. Meyer) were as follow. 1. Optimum light intensity for apparent photosynthesis at $25^{\circ}C$ was 25Klux($1.35mgCO_2/dm^2{\cdot}hr$) for two years old ginseng grown in pot and 30Klux($1.94mgCO_2/dm^2{\cdot}hr$) for the six years old palmate cut leaves. Optimum temperature at 25Klux was $15^{\circ}C$ ($1.40mgCO_2$) for the 2 years old and 20 to $22^{\circ}C$ ($2.03mgCO_2$) for the 6 years old. 2. Dark respiration increased almost linearly with the increase of air temperature till $25^{\circ}C$ (2.6times between $16^{\circ}C$ to $25^{\circ}C$ for the 6 years old and 1.8 times between $15^{\circ}C$ to $25^{\circ}C$ for the 2 years old). Dark respiration was 11.1 % of net photosynthesis at $16^{\circ}C$, 17.8% at $25^{\circ}C$ for the 6 years old and 40% at $15^{\circ}C$, 64.7% at $25^{\circ}C$ for 2 years old. 3. Stomata appeared only in abaxial surface (lower epidermis) and stomatal frequency was $37per\;mm^2$. 4. Above results together with other informations quoted here strongly suggest that air temperature is much better criteria than light intensity for the improvement of shading roof material and shading construction. That is to promise maximum light intensity unless air temperature is above $25^{\circ}C$.

• Current Research on Agriculture and Life Sciences
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• v.31 no.1
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• pp.45-50
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• 2013

Mutagenesis is used to study gene function and obtain new genetic resources for plant breeding. Soybean is an important oil crop in the world. Thus, to find new genetic resources, a mutation population was developed from the soybean cultivar Pungsannamul using 0.3% ethyl methane sulfonate. The variation of fatty acids was then evaluated among 892 M4 generation mutant lines selected from 3,774 mutant lines. While the wild type Pungsannamul showed 11.6, 3.4, 23.8, 53.3, and 7.8% for palmitic, stearic, oleic, linoleic and linolenic acid, respectively. the fatty acid variations in the mutant lines ranged from 7.4 to 19.7%, 2.2 to 13.0%, 14.7 to 49.0%, 31.8 to 63.9%, and 3.9 to 15.9% with an average of 10.8, 3.8, 25.3, 52.0, and 8.1% for palmitic, stearic, oleic, linoleic and linolenic acid, respectively. Thus, two mutation lines with higher plamitic acid, PE1542 (17.1%) and PE3058 (17.0%), one line with lower stearic acid, PE2166 (1.9%), one line with higher stearic acid, PE977 (12.7%), two lines with higher oleic acid, PE450 (44.4%) and PE2742 (47.7%), and two lines with lower linolenic acid, PE594 (4.6%) and PE1690 (3.7%), were selected from this study. The newly selected fatty acid variants will be good genetic sources for gene function analyses and breeding soybean varieties with altered fatty acids for various industrial and human food applications.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.140-148
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• 2018

As the global warming causing desertification increase, there is growing concern about damage of crops. It was to investigate how the treatment with hydrogen peroxide before leaf development affects the growth and yield of sorghum for minimizing a damage of crops to drought. The germination experiment was conducted at alternating temperature of $25^{\circ}C/20^{\circ}C$(12 hr/12 hr) under water stress condition of 0 ~ -0.20 MPa adjusted with PEG solution containing 0 and 10 mM $H_2O_2$. In order to know the effect of foliar application of hydrogen peroxide on the growth of sorghum, 10 mM hydrogen peroxide was treated to leaves at 3-leaf stage of sorghum growing in greenhouse conditions. Seed germination rate was increased by 20% in hydrogen peroxide treatment as compared to the Control. under water stress conditions (-0.15 ~ -0.20 MPa). The length of seedlings was also on the rise by the hydrogen peroxide treatment. In the greenhouse pot experiment, the morphological characteristics (plant height, stem diameter, leaf length, and leaf number) and physiological characteristics (chlorophyll content, chlorophyll fluorescence (Fv/Fm), stomatal conductance) were higher in the plants treated with hydrogen peroxide under the drought stress condition than those of plants of $H_2O$ treatment. Experiment conducted with the soil moisture gradient system showed that the foliar application of hydrogen peroxide increased photosynthetic ability of sorghum plant with respect to SPAD value and stomatal conductance and rooting capacity (root weight and root length) under drought condition. Generally, hydrogen peroxide treatment in sorghum increased the tolerance to drought stress and maintained better growth due to ameliorating oxidative stress.

• Korean Journal of Environmental Agriculture
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• v.29 no.4
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• pp.328-335
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• 2010

This study was conducted to determine effects of soil pH and form of nitrogen fertilizers on tomato growth and chemical properties of greenhouse soil using ferigation system. Tomato (Lycopersicon esculentum Mill. cv. Superdoterang) were grown for three months in 18 L pots filled with two soil (pH 6.8 and pH 8.7). 4 different nitrogen fertilizers (urea, ammonium nitrate, ammonium sulfate, and potassium nitrate) were fertigated with different concentrations of 0, 10, 50, and 100 mg N/L during tomato cultivation. Soil pH 8.7 decreased yield and chlorophyll fluorescence compared with soil pH 6.8. Yield at soil pH 8.7 increased by ammonium nitrate and ammonium sulfate fertigation. Soil pH 6.8 induced increment of yield by nitrogen concentration than form of nitrogen fertilizers. Soil pH after cultivation of tomato decreased by application of ammonium nitrate and ammonium sulfate. Soil EC by 100 mg N/L application of ammonium sulfate was twice as much as other fertilizers. Form of nitrogen fertilizer had less effect on concentration of soil $NH_4^+$-N and $NO_3^-$-N in soil but the concentrations slightly reduced at pH 8.7. These results indicate that application of urea and ammonium nitrate for a nitrogen source of fertigation has little affects on soil chemical properties before and after tomato cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.515-521
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• 1997

Tobacco plant was grown for 40 days hydroponically in nutrient solutions composed of different forms of nitrogen, like NO$_3$$^{[-10]}$ -N, NH$_4$$^{＋}$-N, and a mixed formulation of NO$_3$$^{[-10]}$ -N and NH$_4$$^{＋}$-N. Uptake response, nitrate reductase, and glutamine synthetase activity at growth stage were investigated to understand the basic knowledge of nitrogen metabolism. The better growth of shoot and root was observed in the mixed nutrient solution than NO$_3$$^{[-10]}$ -N or NH$_4$$^{＋}$-N, alone. The plant growth in NH$_4$$^{＋}$-N nutrient solution was poor due to ammonium toxicity. The pH of nutrient solution containing NO$_3$$^{[-10]}$ -N increased up to 40 days after transplanting. But the pH of solution containing NO$_3$$^{[-10]}$ -N decreased drastically to 3.42 at 20 days after transplant. The pH in the mixed formulation dropped to pH 3.64 at 30 days after transplant and showed re-increase. It is assumed that nitrogen of NH$_4$$^{＋}$-N form was taken up preferentially at early stage and NO$_3$$^{[-10]}$ -N form was taken up preferentially at middle stage in the treatment with the mixed solution. The result indicates that the relative proportion of nitrogen forms affected the uptake patterns at each growth stages. The contents of chlorophyll and soluble protein were high with the mixed solution. Total nitrogen content was the highest in NH$_4$$^{＋}$-N solution and the content also increased by the application of the mixed type of nitrogen. The amount of nitrate in leaves was high in NO$_3$$^{[-10]}$ -N treatment and the amount of ammonium was high in NH$_4$$^{＋}$-N treatment. The activity of nitrate reductase or glutamine synthetase was highest in the leaves grown in mixed nutrient solution than in those with any other single of nitrogen form.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.217-223
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• 2011

This study was conducted to examine the effect of several pretreatments and holding solutions on the vase life and quality of cut Polygonatum odoratum var. pluriflorum for. variegatum stems native to Korea. Postharvest foliar spray of 200 $mg{\cdot}L^{-1}$ $GA_3$ or 200 $mg{\cdot}L^{-1}$ BA + 200 $mg{\cdot}L^{-1}$ $GA_3$ markedly extended the vase life of cut stems of Polygonatum odoratum var. pluriflorum for. variegatum. These treatments maintained high chlorophyll contents as compared with the control (distilled water) during senescence of cut stems. Postharvest pretreatment (dipping) with 100 $mg{\cdot}L^{-1}$ $GA_3$ or 3% sucrose + 200 $mg{\cdot}L^{-1}$ 8-hydroxyquinoline sulfate (HQS) + 100 $mg{\cdot}L^{-1}$ $GA_3$ for 16 hours extended vase life of cut stems 2.5 times as long as nontreated control. Holding solution of 2% sucrose + 200 $mg{\cdot}L^{-1}$ HQS + 100 $mg{\cdot}L^{-1}$ $GA_3$ significantly extended vase life by 3.5 times and increased fresh weight as compared with control. $GA_3$ were very effective on preventing leaf yellowing of cut Polygonatum odoratum var. pluriflorum for. variegatum stems. Postharvest foliar spray of 200 $mg{\cdot}L^{-1}$ $GA_3$ or holding solution of 2% sucrose + 200 $mg{\cdot}L^{-1}$ HQS + 100 $mg{\cdot}L^{-1}$ $GA_3$ markedly extended vase life and improved quality of cut stems of Polygonatum odoratum var. pluriflorum for. variegatum.

• Journal of the Korean Society for Marine Environment & Energy
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• v.10 no.3
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• pp.127-137
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• 2007

To confirm whether or not the Electrochemical Disinfection System (EDS) meet with the D-2 regulation established by IMO (International Maritime Organization), the biological treatment efficacy of the EDS was assessed using three groups of natural marine plankton (bacteria, $10-50\;{\mu}m$ and $>50\;{\mu}m$ sized organisms). Influent water was passed through the EDS under the flow velocity ($23.8\;m^3/hr$) and test design was consisted of control (no treatment) and experimental (10 ppm and 30 ppm) condition for total residual chlorine (TRC). And the biological condition of the influent water followed the standards established by the guidelines for the approval of ballast water management systems. The disinfection efficacy of the $10-50\;{\mu}m$ sized organisms (phytoplankton) was assessed by three kinds of measurements using photomicroscope, epifluorescence microscope and fluorometer (fumer Designs 10-AU). After being passed through the EDS, all motile phytoplankton lost their motility under photomicroscope, the colour of chlorophyll fluorescence fumed from red into green under epifluorescence, and the high chlorophyll fluorescence (Expt. 1: 6.95, Expt. 2: 7.11) detected by fluorometer decreased into value not detected. These results indicated phytoplankton community was totally killed after electrochemical disinfection treatment. Survivorship of the larger organisms than $50\;{\mu}m$ was determined based on the appendage's movement under a stereomicroscope. Natural assemblage collected from ambient seawater was killed shortly after being passed through the EDS, whereas some Artemia remained alive. However, no live Artemia was found after 24 hour further exposure to each TRC concentration (10 and 30 ppm) under darkness. After electrochemical treatment, the target bacteria such as aerobes, coliform and Escherichia coli were completely killed on the basis of CFU (colony forming unit) on Petrifilm plate ($3\;M^{TM}$) after 48 hr incubation. Moreover, no regrowth was found in the three groups of plankton during five days under additional exposure to the treated water. These results indicated that the disinfection efficiency of the EDS on the three groups of plankton satisfy D-2 regulation.

• The Korean Journal of Malacology
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• v.29 no.1
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• pp.51-63
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• 2013

To assess the effect of environmental factors on the sustainability of cultured production shellfish, we investigated the habitat characteristics of tidal flat (Namhae-po in Taean). We measured the physiochemical parameters (temperature, salanity, pH, dissolved oxygen and nutrients) and the geochemical characteristics (chemical oxygen demand, ignition loss, C/N ratio and C/S ratio). Surface sediments were collected from several site of tidal flat to examine the geochemical characteristics of both the benthic environment and heavy metal pollution. The grain size for research area of tidal flat were similar at the ratio of silt and clay in comparison with the other site of it. The C/N ratio was more than 5.0, reflecting the range arising from the mix of marine organism and organic matter. The C/S ratio (about 2.8) showed that survey area had anoxic or sub-anoxic bottom conditions. The enrichment factor (Ef) and index of accumulation rate (Igeo) of the metals showed that those research areas can be classified as heavily polluted, heavily to moderately polluted, or more or less unpolluted, respectively. Adult surf clam (Mactra veneriformis) density was highest at St. 2 (middle part of the Namhae-po), on the other hand, surf clam spat density was highest at St. 3 (lower part of the Namhae-po). Heavy rain, terrigenous suspended clay with fresh water from neighboring agricultural land, and severe high air temperature during summer could be thought as detrimental causes of spat and adult mortality in Namhae-po tidal flat. We suggested that the growth of shellfish in the tidal flat was effected by the various environmental conditions, so an improvement in the cultured method was needed.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.1-7
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• 2006

This study was conducted to determine chemical compounds and antioxidant in Aruncus dioicus. Further, effects of cultural practices such as the shading conditions on the general compounds, essential oils and antioxidants. The chemical compounds and antioxidant activity of edible extracts on the shading cultivation and harvesting time were measured by crude fiber, pigments, higher fatty acids, essential oils and DPPH free radical scavenging ability and activities of SOD and POD in edible shooting parts of Aruncus dioicus. The contents of crude fiber, total chlorophyll and total carotenoids in extracts of edible shooting by shading cultivation and later harvesting were higher than those of non-shading cultivation and early harvesting. Phenolic compounds such as benzoic acid were identified as the aromatic compounds in the edible extracts of Aruncus dioicus. The contents of azulene, benzaldehyde and linalool among those compounds on the cultivation conditions increased in shading cultivation. $IC_{50}$ values of DPPH free radical scavenging activities were high from 6.644 to $14.499{mu}g/m{\ell}$ in extracts of edible parts and those of edible shooting parts was high such as edible shooting cultivated by 60% shading, 30% shading and non-shading, respectively. The activity of POD and SOD in seeds was lower than that of edible shooting and that by shading cultivation was high in extracts cultivated by non-shading. POD activity of extracts on harvesting time was high in earlier harvesting but SOD activity was low. The numbers of isozyme pattern of POD and SOD in seedling showed 7 bands and 3 bands, respectively, especially, bands of POD and SOD in the first year-growing plant did not show and show a difference according to plant positions, respectively.