• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.39-45
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• 2000

Two maize transposable elements, immobilized Ac (iAc) and Ds, have been introduced into the genome of a diploid potato clone (Solanum tuberosum Group Phureja clone 1.22). The iAc is a modified Ac that is supposed to be unable to transpose but is expected to trans-activate the transposition of a Ds that is unable to transpose by itself. When the leaf and stem explants of in vitro shoots of the clone 1.22 were inoculated with Agrobacterium tumefaciens strains harboring binary vectors containing the iAc and the Ds, calli were formed from the explants on media containing 50 mg/L of kanamycin, and shoots were regenerated from the calli. The regenerated shoots formed roots when cultured on media containing 100 mg/L of kanamycin, whereas untransformed shoots did not form roots on the same media. The PCR amplification of the DNA's from the transgenic plants confirmed that the iAc and the Ds elements were introduced into the potato genome of 1.22.

• Journal of Aquaculture
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• v.13 no.1
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• pp.79-85
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• 2000

The effects of estrogen and androgens on Vg gene expression were examined in primary hepatocyte culture and livers of the immature male trout. Specific primers of Vg cDNA were designed with already reported Vg gene nucleotide sequences. PCR product was sequenced and verified with Vg cDNA of rainbow trout. Total RNA was extracted from the cultured hepatocytes and livers of steroid-treated rainbow trout and then it was analyzed by reverse transcriptase- polymerase chain reaction (RT-PCR) analysis. The Vg mRNA and Vg protein synthesis were increased in rainbow trout in vivo and in vitro with E$_2$ and methyltestosterone (MT) There were dose and time-related effects of E$_2$ and MT on vitellogesis. Androgens such as progesterone androsterone and testosterone also stimulated Vg mRNA expression in vitro. The results show that androgens as well as E$_2$ can induce expression of Vg mRNA in trout in vivo and in vitro.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.65-65
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• 2018

복분자(Rubus coreanus Miquel)는 anthocyanin, phenolic acid, tannin, flavonoid, stilbenoid와 같은 생리활성물질이 풍부하게 존재하는 것으로 보고되었으며 항염증, 항암활성, 항산화효과 등 다양한 생리활성에 대한 효능이 있어 건강식품으로써 애용되고 있을 뿐만 아니라 약용성 건강보조식품 등으로 각광받고 있다. 생물전환(Bioconversion)은 미생물 또는 효소의 생물학적 촉매 기반 천연소재의 생리활성 물질기능성, 생체이용률, 안전성을 증대시키기 위한 방안으로 많은 연구가 진행되고 있으며 아울러 식품, 의약품, 화장품 등 다양한 분야에서 활성화 되고 있다. 본 연구는 불가사리 발효액으로부터 유산균을 분리하였으며, 유전학적 특성을 확인하기 위하여 16S rDNA 염기서열을 분석하였다. 전북 고창에서 수확된 복분자를 분말상태로 유산균과 발효공정을 수행하였으며, 복분자의 최적 추출조건 선정과 발효공정 전 후의 활성을 관찰하였다. 발효공정 후 추출물의 기능성 평가를 진행하기 위하여 DPPH radical scavenging activity, total polyphenol 함량을 확인하여 항산화 효능 및 유효성분 함량을 평가하였다. 또한 대식세포인 Raw 264.7을 사용하여 MTT assay, Nitric oxide (NO) 생성 억제 효능을 확인하여 세포독성 및 항염증 활성을 평가하였다. 실험결과, 발효기간이 다른 불가사리 비료발효액으로부터 16 종의 다양한 균주를 확보하였으며, 생물전환 공정에 유용 균주를 선정하기 위하여 복분자 분말의 발효공정을 실시한 결과 3 종의 유산균 처리군에서 무처리군 대비 DPPH radical 소거능 및 polyphenol 함량이 증가됨을 확인하였다. 그 중 가장 우수한 활성을 나타내는 균주를 16S rDNA 염기서열 분석한 결과 Lactobacillus coryniformis A6-4로 확인되었으며, 발효공정 후 항산화 활성은 무처리군 대비 약 115%, polyphenol의 함량은 무처리군 대비 약 121%로 증가됨을 확인되었다. 또한 발효공정 후 독성활성이 감소되는 경향을 확인되었으며, 항염증 활성이 유의적으로 증가됨을 확인하였다.

• Elastomers and Composites
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• v.36 no.2
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• pp.94-101
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• 2001

In this work, the effect of NE plasma treatment generated by radio-frequency was investigated in acid-base surface values, ion adsorption characteristics, and surface free energies of carbon blacks. As the results, it was clearly found that the obvious improvement of the treatment is in the London dispersive component (${\gamma}s^L$) of surface free energy of carbon blacks. Also both electron-acceptor ( ${\gamma}s^+$) and -donor (${\gamma}s^-$) parameters for the specific component (${\gamma}s^{sp}$) were also increased with increasing the treatment time, resulting in increasing the surface functional groups of the carbon blacks studied, together with a consequence of the increases of acidic and basic surface functional groups, ion exchange, zeta potential, and ion mobility.

• Journal of Korean Society of Forest Science
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• v.83 no.3
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• pp.280-285
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• 1994

This study was conducted to investigate the effects of acid deposition on forest soil, major five Korean forest soils(Brown, Dark red, Gray brown, Red and Yellow, and Volcanic ash forest soils) The samples were subjected to receive 1200mm($100mm{\times}12$ times) of artificial acid rain adjusted to pH5.6, 4.0, 3.0 and 2.0. The results obtained of major importance are summarized as follow ; 1. Ca appeared mostly affected at pH treatment of 2.0, while less affected by other pH treatments. Leaching of Ca rapidly increased with increasing of artificial acid rain acidity and application times in Dark red forest soil. 2. In the cases of Mg, K and Na, they showed gradual increase with the addition of artificial acid rain. Mg and Na losses showed similar leaching patterns, but they didn't show difference among the five forest soils. 3. Exchangeable canon concentrations in the soil leachates, which looked slightly different among the five forest soils, were the highest in pH2.0 treatment. Hydrogen ion comsumption capability by exchangeable canon was the highest in Dark red forest soil followed by Volcanic ash, Red and Yellow, Gray brown and Brown forest soils when artificial acid rain were treated.

• Korean Journal of Poultry Science
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• v.17 no.4
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• pp.310-317
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• 1990

An experiment was conducted to investigate the effects of adding extra dietary salt on the blood acid－base balance, water intake and egg quality in heat stressed layers. Eighteen 44－wk－old brown commercial layers(Dekalb Warren) were randomly alloted to 0.25 and 0.75% dietary salt treatments, with nine replicates per treatment and one bird per replicate. After 3 d of preliminary period at 13~$16^{\circ}C$(normal temperature), layers were subjected to 3 d of collection period. Then the room temperature was raised to 33－$35^{\circ}C$f(hot temperature), and another 3 d of collodion period was followed. The experiment was designed as a $2\times2$ factorial and analyzed as suck The ANOVA test and comparison among treatment means were done at 5% probability levels. Results obtained were summarized as follows. L The heat stressed layers decreased feed intake, and increased water intake and excreta moisture content significantly. The addition of extradietary salt significantly increased excreta moisture content 2. The heat stressed layers showed significantly higher blood pH and lower $pCO_2$ values than the control bird However, the addition of extra salt did not change any acid－base variables. 3. The egg weight and shell quality decreased significantly, and haugh unit increased significantly by the heat stress. However, these values were not affected by the salt addition. In summary, the heat stressed layers displayed respiratory alkalosis and poor egg quality, as expected However, the addition of ex03 salt to the diet failed to alleviated the heat stress in this layer experiment.

• Korean Chemical Engineering Research
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• v.43 no.1
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• pp.60-65
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• 2005

The electrosorption of U(VI) from waste water was carried out by using activated carbon fiber(ACF) felt electrode in a continuous electrosorption cell. In order to enhance the electrosorption capacity at lower potential, ACF felt was chemically modified in acidic, basic and neutral solution. Pore structure and functional groups of chemically modified ACF were examined, and the effect of treatment conditions was studied for the adsorption of U(VI). Specific surface area of all ACFs decreases by this treatment. The amount of acidic functional groups decreases with basic and neutral salt treatment, while the amount increases a lot with acidic treatment. The electrosorption capacity of U(VI) decreases on using the acid treated electrode due to the shielding effect of acidic functional groups. Base treated electrode enhances the capacity due to the reduction of acidic functional groups. The electrosorption amount of U(VI) on the base treated electrode at -0.3 V corresponds to that of ACF electrode at -0.9 V. Such a good adsorption capacity was not only due to the reduction of shielding effect but also the increase of $OH^-$ in the electric double layer on ACF surface by the application of negative potential.

• Development and Reproduction
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• v.5 no.2
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• pp.107-114
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• 2001

The recent reports that endocrine disruptors(EDs) bring about abnormalities in reproductive organs and functions of invertebrates suggest that mammals be affected by the EDs. The present study examined the influence of 2-bromopropane(2-BP) by looking at the sexes of litters in mouse. The expression of sex-related genes during sex differentiation was also investigated in the fetus of mouse. The male and female mice were infused with 2-BP for 3 weeks before mating. The litters were sexed at the weaning time from the 4 different groups. The sex-related genes were identified by RT-PCR from the fetuses at gestation 10 days. The sequences of the genes were analysed by comparing to those of other animals. The mean numbers of litters survived by the weaning time were slightly reduced in the only group of both female and male mice treated with 2-BP. The female litters were greater than male litters in the only group of female treated with 2-BP. The other groups showed male litters greater than female litters. The sex-related genes, SRY, DAX1, SF1 , and AMH genes were identified and sequenced, showing 416, 466, 326, 389 base pairs, respectively. All of the genes had the homology of 89～90％ with rat and 81～92％ with human within the range of bases identified. They were expressed at the time of sex determination. Therefore, it appears that 2-BP somewhat affects the reproductive activity of adult mouse. Influence of 2-BP on the reproductive function is expected to be studied through the expression of the sex-related genes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.477-484
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• 2019

Several types of scavenger receptors, including the Collectin-Placenta 1 (CL-P1) receptorthat is present in mammals, are molecules that are expressed on the surfaces of endothelial cells, macrophages and smooth muscle cells. These molecules are cell surface glycoproteins that can be conjugated to oxidized low density lipoprotein (oxLDL). Among these molecules, the effect of quercetin on CL-P1 activation has been confirmed. Quercetin is known as an antioxidant that stops oxidation because it acts to remove free radicals that are responsible for the oxidation reaction. In this study, fragments from the transcription start site of the mouse CL-P1 gene promoter to the -500th base were cloned using DNA polymerase. These fragments were then introduced into macrophage like RAW264.7 cells and fibroblast-like NIH3T3 cells to study the effect of quercetin on the CL-P1 gene expression. As a result, we found that bases ranging from -250 to -350 in the anterior part where gene expression starts are important for producing CL-P1 protein. Among them, the DNA mutation experiments we performed confirmed that the E2F binding sites are critical for producing the CL-P1 protein? In addition, when quercetin was added to the RAW264.7 culture medium, which was a culture of adherent cells, observedthe phenomenon of the cells falling off from the surface of the culture container.

• The Magazine of the IEIE
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• v.29 no.3
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• pp.58-70
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• 2002

약 1그램의 DNA는 10/sup 21/개의 DNA 염기를 가지며 이것은 10억 terabits의 정보저장 능력에 해당한다. 분자정보처리기술(molecular information processing technology)은 DNA나 단백질과 같은 거대분자의 초미세구조, 초고집적도, 초에너지 소비특성에 기반하여, 용액상에서 아보가드로수(6×10/sup 23/) 규모의 분자들이 3차원 화학 반응에 관여하는 초병렬분산 정보 저장 및 처리 능력을 활용하는 차세대 IT 기술이다. 이는 또한 생체분자의 특성을 나노수준에서 활용하는 정보기술이라는 점에 있어서 BT+NT+IT의 융합기술이며 그 기술적 산업적인 파급 효과가 아주 큰 특징이 있다. 본고에서는 현재 분자정보 처리 기술의 원리 및 문제점을 설명하고 이의 해결을 위한 5가지 핵심 요소 기술을 도출한다. 최근 세계적인 연구 동향을 살펴보고, 국내의 기술 기반 및 국제 경쟁력을 분석하며, 궁극적인 경제성 확보를 위한 산업화 방안을 단기(3-5년), 중기(5-10년), 장기(10-20년)의 3단계로 나뉘어 제안한다.