• The korean journal of orthodontics
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• v.32 no.2 s.91
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• pp.129-142
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• 2002

The purpose of this study was to evaluate the effects of caffeine and calcium on the activities of the osteoblastic cell from mouse calvaria. The author cultured osteoblastic cells obtained from the mouse calvaria and were divided into three groups : the caffeine-treated, the calcium-treated and the combine-treated group. In caffeine-treated group, the cell toxicity was measured by MTT assay at 1, 2 and 4 days after treatment of caffeine. In all groups, the densities of the mineralized bone nodules were measured by imaging analyzer after Von Kossa staining. The alkaline phosphotase (ALP) activities were measured at 2, 7, 14, 21 and 28 days and the interleukin-1 ${\beta}$ activities at 48 hours after treatment of caffeine and calcium. The measurements were statistically executed with ANOVA test and the results were as follows. 1. The cellular toxicity of the caffeine increased with the concentration of caffeine during the incubation period. 2. The maximum densities of mineralization were observed at 0.2 mM caffeine-treated group, 1.2 mM calcium-treated group, 0.1 mM caffeine and 1.8 mM calcium-treated group. 3. The activities of ALP were peaked at 14 days at calcium-treated group as no-treated. But, the activities of ALP increased with concentrations of caffeine at caffeine-treated group. At combine-treated group, the act of ALP were peaked at 24 days at 1.2 mM, 1.8 mM calcium-treated group, But decreased at 2.5 mM calcium-treated group. 4. The activites of the IL-1 ${\beta}$ were increased significantly at 0.2 mM caffeine-treated group, 1.8 mM calcium-treated group and 0.1 mM caffeine and 1.8 mM calcium-treated group. But, they were decreased at all groups of high concentration.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.4
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• pp.337-342
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• 1984

224 profiles representing 19 soil series were subjected to multiple regresion analysis to determine the relative contribution of organic matter(OM) and clay content to total cation exchange capacity(CEC) in sandy soil. The independent variables were OM and clay, with the dependent variable CEC. Simple correlation coefficients showed high significance at CEC-OM and CEC-clay. The partial regression coefficients indicated that CEC for each gram of OM was calculated to be 0.549 and 1.351 meq of top and subsoil. The clay contributions of top and subsoil were 0.247 and 0.226 meq, respectively. The standard partial regression coefficients appeared that clay content was 1.23 times as important as orgnic matter in predicting CEC.

• Proceedings of the KIEE Conference
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• 2001.07c
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• pp.1869-1871
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• 2001

DNA 센서의 중요한 역할 중의 하나는 염기서열을 분석함으로써 유전적인 질병이나 돌연변이를 찾아낸다는 점이다. 염기서열 분석법으로 질량, 광학, 전기 화학적 측정법 등이 있는데, 그 중 전기 화학적 측정방법이 타 방법에 비해 간편하고 비용도 저렴해서 전망이 매우 밝다. 전기 화학적 측정을 위해서는 전극의 표면 처리 공정과 전극 표면에서의 DNA immobilization, hybridization 공정 및 전기적 신호를 발생시키는 intercalator, 그리고 전기적 신호 검출을 위한 측정 장비가 필요하다. 본 논문에서는 전극의 표면 처리 물질로서 2-mercaptoethanol을 사용했고 double strand DNA의 intercalator로써 methylene blue를 사용했으며, methylene blue의 환원 전류값을 측정하여 double strand DNA를 bare Au 또는 single strand DNA와 구분할 수 있었다. 이러한 연구 결과를 토대로 하여 전기 화학적 신호 검출을 이용한 DNA 센서의 가능성과 개발 방향을 제시하고자 한다.

• Journal of Life Science
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• v.14 no.1
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• pp.38-44
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• 2004

Two genes, SinIFS1 and SinIFS2 from Korean soybean cultivar, Sinpaldalkong known as one of isoflavonerich cultivars, were cloned with PCR and degenerate primers. The sequences of two genes were analyzed with previously reported IFS genes of leguminous plants and their expression pattern in various environmental conditions was surveyed. The genomic clone of SinIFS1 contained 1,828bp nucleotides and encoded a polypeptide of 521 amino acids, and 1912bp nucleotides and a polypeptide of 521 amino acids for SinIFS2. Both genes included several conserved motifs, oxygen binding and activation (A/G-G-X-E/D-T-T/S), ERR triad (E...R....R), and heme binding (F-X-X-G-X-R-X-C-X-G) domain, which are typical in any member of cytochrome P45O superfamily. Very high sequence homology (>98%) was observed in the comparison with other IFSs of legumes. In the northern blot analysis to check the expression and increase of SinIFS1 to various environmental renditions (low temperature, light, dark, UV, and fungal elicitor), the most significant induction, more than 6 times of transcript level compared to the dark treatment as a control, was observed from the fungal elicitor treatment. The next up-regulated expression was from UV treatment (4${\times}$), low temperature and light conditions.

• KSBB Journal
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• v.19 no.4
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• pp.274-283
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• 2004

Six lumbrokinase (LK) fractions from Lumbricus rubellus lysates were purified by a series of column chromatographies. The molecular weights of the six LK fractions appeared to range from 24.6 to 33.1 kDa. In the experimental model of rat venous thrombosis, the thrombus weight and PAI activity decreased significantly when the LK was administered orally. However, the activities of APTT, PT and plasmin showed a significant increase. The aggregation of rat platelets pretreated with various LK doses was inhibited by thrombin, and the MDA generation decreased. The rat thoracic aorta and mesentric arteries contracted with phenylephrine relaxed due to the treatment of the LK fractions. These results suggest that the fibrinolytic effects of LK were mediated not only by proteolytic activity, but also by the inhibition of platelet agregation and the relaxation of blood vessels. It is concluded that the LK may be useful as a hemolytic agent for treatment of fibrin clot.

• Korean Chemical Engineering Research
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• v.43 no.5
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• pp.632-636
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• 2005

In this work, the effect of chemical treatments on surface properties of SiC was investigated in thermal and mechanical interfacial behaviors of SiC/PMMA nanocomposites. The acid/base value, contact angles, and FT-IR analysis were performed for the study of surface characteristics of the SiC studied. The thermal stabilities of the SiC/PMMA nanocomposites were investigated by thermogravimetric analysis (TGA). Also the mechanical interfacial properties of the composites were studied in critical stress intensity factor ($K_{IC}$) and critical strain energy release rate ($G_{IC}$) measurements. As a result, the acidically treated SiC (A-SiC) had higher acid value than that of untreated SiC (V-SiC) or basically treated SiC (B-SiC). The acidic solution treatment led to an increase in surface free energy of the SiC, mainly due to the increase of its specific component. Thermal and mechanical interfacial properties of the SiC/PMMA nanocomposites, including initial decomposition temperature (IDT), $K_{IC}$, and $G_{IC}$ had been improved in the acidic treatment on SiC. This was due to the improvement in the interfacial bonding strength, resulting from the acid-base interfacial interactions between the fillers and polymeric matrix.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.2
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• pp.83-88
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• 1973

The newly devised column set is better than the conventional method with centrifuge, suction filter instruments for saturating of the salt and washing out the excess of salt for C. E. C. determination in soils. 1. The new method has a high significance with one suction filter set. 2. The new method deminishes the time and effort of analysis by one third. It can analyze many samples at a time. 3. It is able to analyze without expensive instruments (e. g. vaccum pump, shaker, suction apparatus and centrifuge).

• Proceedings of the Korea Information Processing Society Conference
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• 2001.10a
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• pp.105-108
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• 2001

cDNA(complementary DNA)를 복제(cloneing)하여 염기 서열화 한 EST(Expressed Sequence Tag) 데이터는 여러 생물체들의 염기서열 정보들과 비교를 통해 유사점을 찾거나 기능적 부위 검색을 통해 유전자 기능을 추정한 수 있어 기능 유전체 연구에 많이 사용되고 있다. EST 데이터를 식물은 특정종(Species)별로, 동물의 경우 종의 조직별로 클러스터링 함으로써 아직 알려지지 않은 종의 유전자를 밝혀낼 수 있음은 물론 유전자의 발현에 따른 단백질의 기능도 알아낼 수 있다. 따라서 이 논문에서는 NCBI에서 flatfile 형태로 제공하는 EST 데이터를 분석하여 관계형 데이터베이스로 모델링하고 구축하였다. 또한 EST 데이터의 효율적인 사용을 위하여 데이터를 특정 종의 조직별로 클러스터링하여 제공하는 시스템을 설계하고 구현하였다.

• Proceedings of the Korea Information Processing Society Conference
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• 2002.11a
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• pp.323-326
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• 2002

DNA 컴퓨팅은 생체 분자들이 갖는 막대한 병렬성을 이용하여 조합 최적화 문제에 적용하는 연구가 많이 시도되고 있다. 특히 TSP(Travelling Salesman Problem)는 간선에 대한 가중치 정보가 추가되어 있기 때문에 가중치를 DNA 염기 배열로 표현하기 위한 효율저인 방법들이 제시되지 않았다. 따라서 본 논문에서는 DNA 컴퓨팅에 DNA 코딩 방법을 적용하여 정점과 간선을 효율적으로 생성하고 표현된 DNA 염기 배열의 간선에 실제간을 적용하여 가중치 정보를 계산하는 ACO(Algorithm for Code Optimization)를 제안한다. DNA 코딩 방법은 변형된 유전자 알고리즘으로 DNA 기능을 유지하며, 서열의 길이를 줄일 수 있으므로 최적의 서열을 생성할 수 있는 특징을 갖는다. 실험에서 ACO를 TSP에 적용하여 Adleman의 DNA 컴퓨팅 알고리즘과 비교하였다. 그 결과 초기 문제 표현에서 우수한 적합도 값을 생성했으며, 경로의 변화에도 능동적으로 대처하여 최적의 결과를 빠르게 탐색할 수 있었다.

• Proceedings of the Korea Information Processing Society Conference
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• 2012.11a
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• pp.1158-1161
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• 2012

식물 염기 서열 분석은 동물에 비해 유전체가 길고 데이터 양이 많아 특별한 전략을 요구하고 있다. 본 논문은 FCLA 전략을 지원하기 위한 데이터 분석 플랫폼의 개발에 대해 서술한다.