• The Korean Journal of Mycology
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• v.23 no.3 s.74
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• pp.226-231
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• 1995

Tile effects of addition of eggshell to sawdust substrate for the growth of F. velutipes were investigated. Eggshell used in this study contained 20.7% C, 0.81% N, 2530 ppm $P_2O_5$ and 44.37% Ca. The addition of eggshell resulted in the increase in bulk density and decrease of moisture content of the substrate. The addition of eggshell significantly increased the yield of the mushroom fruitbody. The addition rate of 15% (v/v), by 25% and at the rates of 5% and 10%, about 20%. Although the addition of eggshell to substrate did not improve the quality of mushroom, it increased the number of effective stipes as compared to control plot; approximately 13% more than in the control plot.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.5
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• pp.959-970
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• 2000

A laboratory-scale experiment was conducted to investigate the effect of soluble microbial products(SMP) on permeate flux in the submerged membrane separation activated sludge process. Continuous and batch filtration test were operated to understand mechanism of relationship between membrane fouling and SMP. Synthetic wastewater(phenol) was used as a carbon source. Hydraulic retention time(HRT) and mixed-liquor volatile suspended solids(MLVSS) of the reactor were kept at 12 hours and 9.000mg VSS/L, respectively. Batch filtration tests ($J_{60}/J_o$) using the mixed liquor from reactor showed that the increase of accumulated SMP concentration in the reactor caused to the decreasing permeate flux and the increasing of the adhesion matters which form cake and gel layer. The resistance value of cake layer was measured $2.9{\times}10^{10}{\sim}4.0{\times}10^{10}(1/m)$, this value showed more significant effect on flux drop than that of among other resistance layers. Batch phenol-degradation experiment was conducted to observe SMP type expected $SMP_{nd}$ and $SMP_{e}$ (SMP resulted from endogenous cell decomposition), these are non-biodegradable high molecular weight organic matter and playa significant role in permeate flux drop. Also, SMP concentration was accumulated as increased of HRT against flux drop.

• Biomedical Science Letters
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• v.6 no.3
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• pp.209-221
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• 2000

The purpose of this study was to investigate the practical availability of pretense production that can be used at home after isolating Serratia sp.2000-1 which produced extracellular pretense from clinical specimen. Basic production conditions and partial enzymatic characteristics of pretense produced by Serratia sp. 2000-1 was as follows: The kind and concentration of carbohydrate, nitrogen and metal salts for optimal enzyme production condition were each identified as the concentration of 1.5% glucose, 2.0% CSP, and 0.1% CaCl$_2$, and the optimal temperature, time and initial pH for culture were each 3$0^{\circ}C$, 72 hours, and pH 8.0. The final enzymatic yeild that was purified by 3 steps with ammonium sulfate precipitation (45~80%), DEAE-cellulose column chromatography, and Sephadex G-200 gel chromatography was 14.4%, and enzyme inactivity rate increased approximately 291314s. The optimal temperature and pH for purified pretense activity were 35$^{\circ}C$ and pH 7.0~8.0, and purified pretense activity was relatively stable by 4$0^{\circ}C$ at pH 6~10 for 30 min, however heating at 6$0^{\circ}C$ for 30 min, it liminated detectable pretense activity. The pretense activity was activated by $Mg^{2+}$, $Ba^{2+}$, $Ca^{2+}$, Mn$^{2+}$, but inactiviaed by Hg$^{2+}$, Ag$^{2+}$, Cu$^{2+}$, and the pretense activity was inhibited strongly by SDS among enzyme activity inhibitors. Further study is required to evaluate the practical availability of pretense production that can be used at home by isolating Serratia sp. from more clinical specimen and examining pretense more in details.

• Microbiology and Biotechnology Letters
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• v.40 no.2
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• pp.98-103
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• 2012

For screening of useful enzymes producing microorganisms from Meju, we isolated high lipase producing strains and their lipolytic enzyme activities were then tested. The lipolytic enzyme activities of isolated microorganisms were therefore tested on the Y124 strain. The gene sequence analysis of ITS from Y124 strain revealed Yarrowia lipolytica. Lipase production by the Y124 strain was studied in media containing various carbon sources. The Y124 strain drastically increased lipolytic enzyme activity in YPO media containing olive oil, as well as in YPDO media containing both olive oil and glucose. Maximal lipase production was achieved in YPD (yeast extract-peptone-D-glucose) media containing 0.7% olive oil when cultured at $30^{\circ}C$ for 8 hrs. The lipase produced from the Y124 strain showed the highest activity in p-NPO (p-nitrophenyl octanoate ($C_8$)), amongst the various p-nitrophenyl esters.

• Food Science and Preservation
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• v.15 no.2
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• pp.225-230
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• 2008

We investigated the effect of modified atmosphere packaging (MAP) on the removal of astringency and overall quality of astringent persimmons (Diospyros kaki T. cv. Miryangbansi). Persimmons were packaged in 0.08, 0.10, or 0.12 mm low-density polyethylene film and then stored at $0^{\circ}C$ for up to 100 days. Concentrations of $O_2$ and $CO_2$ in the packaging maintained lower and higher as the film thickness increased Weight loss was strongly retarded by all three MAP films. Soluble tannin content decreased as the film thickness increased. The astringency of persimmons disappeared after 60-70 days in 0.12 mm film, after 80 days in 0.10 mm film, but did not disappear after 100 days in 0.08 mm film Loss of flesh firmness and decline in external appearance were slower in persimmons in 0.08 and 0.10 mm films than in those in 0.12 mm film. Our results show that 0.10 mm LDPE films can be effective for maintaining the quality and removal of the astringency of astringent persimmons.

• Journal of the Korean Recycled Construction Resources Institute
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• v.1 no.1
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• pp.81-88
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• 2005

The fly ash has been widely used in the latest to complement the performance and economical efficiency of the concrete which uses only a normal portland cement, the pulp ash gained through the incineration of paper sludge is possible to be used as the material of concrete because it contains the properties similar to the previous fly ash in ingredients and physical characteristics. Therefore, this research has tested physical characteristics by replacing 20% of fly ash used with the paper ash to solve the problem which lowers the early strength caused when the fly ash was used. As a result, it showed that the fluidity becomes lower and the compressive strength becomes increased by using paper ash. In addition, after mixing the paper ash with the fly ash, it showed that time and heating amount of the 2nd peak of the minor heat of hydration affecting the revelation of strength was equivalent to the combination for normal portland cement, and also indicated that the compressive strength for 3 days is superior to the combination of the fly ash. Therefore, if the paper ash having a regular fineness is used, it was effective in improving the early strength of concrete used the fly ash.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.461-464
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• 2002

A Chemoautotroph identified as an Aeromonas sp. strain JS-1 was isolated from fresh water. Aeromonas sp. strain JS-1 used the $H_2$ and $CO_2$ as energy and carbon sources, respectively. Growth characteristics for improving the $CO_2$ fixation rate were examined in batch cultivation. Its results shown that the optimal growth appeared at culture conditions of $35^{\circ}C$, pH 7 and NaCl 0.1%(w/v). Some hydrogen-oxidizing bacteria were reported that the enzyme activity of ribulose 1,5-bisphosphate carboxylase- oxygenase (RubisCO-EC 4.1.1.39), in the key enzyme of the Calvin-Benson cycle. A RubisCO was purified from a chemoautotrophic bacterium, Aeromonas sp. strain JS-1. the enzyme was purified by ammonium sulfate precipitation, DEAE-sepharose CL-6B and gel filtration chromatography. The RubisCO showed that molecular mass was about 560KDa from gel filtration chromatography and nondenaturing PAGE, and the RubisCO was confirmed to consist of $L_8S_8$ enzyme structure by sodium dodecyl sulfate polyacrylamide gel electrophoresis. A large subunit was about 56KDa and small one was about 15kDa. The Km values of the enzyme for ribulose 1,5-bisphosphate(RUBP), $NaH^{14}CO_3$, and $Mg^{++}$ were estimated to be 0.25mM, 5.2mM, and 0.91mM, respectively. The optimum temperature for RubisCO enzymatic activity were $50^{\circ}C$, and the enzymatic activity was stable up to $45^{\circ}C$.

• Journal of Wetlands Research
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• v.19 no.3
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• pp.353-358
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• 2017

As a drinking water source of 26 millions people of Seoul metropolitan region, Han river is one of the most important basin. Managing the Non-point source pollution which is regarded as the main cause of water pollution including refractory organic matter is important thing. This research investigates the runoff characteristics of organic matters from main 3 rivers (South Han, North Han, Kyungan). Water quality measurement items include not only carbon-based TOC (Total Organic Carbon), DOC (Dissolved Organic Carbon), POC (Particulate Organic Carbon) but also R-TOC (Refractory Total Organic Carbon), R-DOC (Refractory Dissolved Organic Carbon), R-POC (Refractory Particulate Organic Carbon) is researched. The research shows that R-TOC takes approximately 61~83% of TOC. Most of the R-TOC is consist of R-DOC (72~77%). Refractory organic matter have a stable runoff characteristics compared to other organic matter. The organic matter concentrations of South Han river and Kyungan river are the highest in spring time and show a gradual decline. The concentrations of Kyunan river is the highest. Kyungan river's small area and the high city land use ratio seem to be the reason. Loading of organic matter in summer time takes the most loading (62~84%). TOC loadings per unit area of each river is Kyungan river ($1.22ton/km^2$), South Han river ($1.01ton/km^2$), North Han river ($0.91ton/km^2$).

• Journal of the Korean Society of Marine Environment & Safety
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• v.22 no.7
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• pp.854-862
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• 2016

In summer 2010, we measured the concentration of dissolved oxygen (DO) and nutrients in the water collected at the bottom of Cheonsu Bay, off the west coast of Korea. We also measured nutrient fluxes across the sediment-water interface by deploying a fully-automated benthic lander, which collected time-series water samples inside a benthic chamber. We confirmed on-going hypoxia in the northern parts of the bay where polluted lake water was discharged. DO content in the water at the bottom was 2 mg/l, compared to 5 mg/l at the mouth of the bay in the south. Nutrient concentrations showed a trend that was opposite to that of DO. The variation of N/P ratios implies phosphate desorption and a release of nutrients caused by hypoxia. The organic carbon oxidation rate and oxygen consumption rate in the northern parts of the bay were about twice as fast as those at the mouth of the bay. Benthic fluxes of nutrients in the northern part of the bay were 4 to 6 times higher than those at the mouth. Our results imply that it is important to understand the role of hypoxia events to make an accurate estimation of material fluxes across the sediment-water interface.

• Clean Technology
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• v.18 no.3
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• pp.229-249
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• 2012

At present, global warming and depletion of fossil fuels have been one of the big issues which should be solved for sustainable development in the future. CCS (carbon capture and sequestration) technology as the post $CO_2$ reduction technology has been considered as a promising solution for global warming due to increased carbon emission. However, the environmental and ecological effects of CCS have drawn concerns. There are needs for noble post reduction technology. More recently, CCU (carbon capture and utilization) Technology, which emphasizes transforming carbon dioxide into value-added chemicals rather than storing it, has been attracted attentions in terms of preventing global warming and recycling the renewable carbon source. In this paper, various technologies developed for carbon dioxide conversion both in gas and liquid phase have been reviewed. For the thermochemical catalysis in gas phase, the development of the catalytic system which can be performed at mild condition and the separation and purification technology with low energy supply is required. For the photochemical conversion in liquid phase, efficient photosensitizers and photocatalysts should be developed, and the photoelectrochemical systems which can utilize solar and electric energy simultaneously are also in development for more efficient carbon dioxide conversion. The energy needed in CCU must be renewable or unutilized one. CCU will be a key connection technology between renewable energy and bio industry development.