• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.05a
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• pp.266-267
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• 2003

최근, 기계의 분석 정밀도가 높아짐에 따라 어류 이석 내 미량원소를 이용한 연구가 활발히 진행되고 있다. 이석은 균형감각기관으로서 칼슘과 단백질의 복합구조로 이루어져 있으며, 어류의 전 생활사에 걸쳐서 성장한다. 이렇게 형성된 어류의 이석내 화학조성은 형성 후엔 변화하지 않는다는 특징을 가지고 있다(Campana,1999). 위와 같은 특징을 바탕으로 이석 내 미량원소 또는 동위원소의 함량비율을 분석하여 어류에 대한 계군분리, 어류의 서식환경 추적, 오염 정도, 그리고 어류의 회유 유무와 경로, 연령사정 등의 연구를 하고 있다(Campana, 1999). (중략)

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.173-174
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• 2000

Yamamoto (1953)가 송사리, Oryzias latipes를 대상으로 성전환에 관한 일련의 연구결과를 발표한 이후 어류를 대상으로 한 성전환 연구보고가 많이 이루어지고 있고(Clemens and Inslee, 1968; Jalabert et al., 1974; Yamazaki; 1976; Goetz et al., 1979; Donaldson and Hunter, 1982; Tanaka, 1988), 현재 어류의 성제어 기술은 연어, 송어, 넙치 등에서 산업적 응용이 가능한 단계에 이르고 있다. (중략)

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2019.10a
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• pp.96-96
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• 2019

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.3
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• pp.183-189
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• 2009

This study was conducted to prepare salmon patty using muscle separated from salmon frame (SPFM) and to investigate the food component characterization. When compared to salmon patty with fillet muscle (SPM), SPFM was lower in the moisture content, while it was higher in crude lipid content. However, no differences in the ash and protein contents between SPFM and SPM were found. Compared to SPM, the Hunter color value in cross section of cooked SPFM was higher in a and $\Delta$E values, while the color was lower in Land b values. Trichloroacetic acid soluble-N content of SPFM was 279 mg/100 g, which was insignificantly different (P>0.05) compared to those of SPM and commercial patty. The hardness of SPFM was 0.44 kg/$cm^2$, which was insignificantly different (P>0.05) compared to that of SPM, while was higher than that of commercial patty. The major fatty acids of SPFM were 16:0 (16.5%), 18:1n-9 (29.2%) and 18:2n-6 (26.1%). The 20:5n-3 and 22:6n-3 were also detected in high composition. The total amino acid content of SPFM was 16.6 g/100 g, which was similar to that of SPM. However, the total amino acid of SPFM was 14% higher than that of commercial patty. From the results of the mineral content, SPFM was higher than that of SPM in Fe and Ca, while the K in SPFM was lower. According to the result of sensory evaluation on the color, flavor and taste, no significant differences in all sensory items between SPFM and SPM were found.

• Ocean and Polar Research
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• v.26 no.3
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• pp.433-438
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• 2004

Stress response to the seawater acclimation in coho salmon (Oncorhynchus kisutch) smolt was investigated. Salt concentration of rearing water was gradually increased for 3 days from freshwater to seawater (30 ppt salt level). The changes of serum concentrations of cortisol as a primary stress indicator, and as secondary indicators, glucose (GLC), lactate (LAC), triglyce.ide (TG), cholesterol (CHOL), sodium ion $(Na^+)$, chloride ion $(Cl^-)$ and enzyme activities (alanine aminotransferase, ALT: aspartate aminotrasferase, AST; lactate dehydrogenase, LDH) were quantified during the acclimation experiment. Among them, cortisol, LAC, TG, CHOL, ALT, AST concentrations showed rapid increase at the first exposure to the 10ppt salt level (day 1), and began to decrease to the constant values after day 2 of adaptation at 20ppt salt level. However, LDH concentration tended to decrease during the whole experimental period. $Na^+\;and\;Cl^-$ showed slight decrease at day 1, and increased to a little bit higher values after day 2 rather than those in freshwater. All the fishes started on taking a food after day 4 of seawater adaptation. From these results, to reduce osmotic shock inducible stress to fish in seawater acclimation, gradual increase of salt levels is recommended.

• Development and Reproduction
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• v.7 no.2
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• pp.81-88
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• 2003

Initiation and activation of sperm motility are prerequisite processes for the contact and fusion of male and female gametes at fertilization. The phenomena are under the regulation of CAMP and $Ca^{2+}$ in vertebrates and invertebrates. Mammalian sperm requires $Ca^{2+}$and cyclic AMP for the activation of sperm motility. Cell signaling for the initiation and activation of sperm motility has been well studied in the ascidians, Ciona intestinalis and C. savignyi and salmonid fishes. In Ciona, whose cell signaling for activation of sperm motility has been established, the sperm-activating and -attracting factor released from unfertilized egg requires extracellular $Ca^{2+}$ for activating sperm motility and eliciting chemotactic behavior of the activated sperm toward the egg. On the other hand, the cyclic AMP-dependent phosphorylation of protein is essential for the initiation of sperm motility in salmonid fishes. A decrease in the environmental Ti concentration surrounding the spawned sperm causes a li efflux and $Ca^{2+}$ influx through the specific $K^{+}$ channel and dihydropyridine-sensitive L-/T- type $Ca^{2+}$ channel, respectively, thereby leading to the membrane hyperpolarization and $Ca^{2+}$ influx. The membrane hyperpolarization synthesizes cyclic AMP, which triggers the luther Process of cell signaling, i.e., cyclic AMP-dependent protein phosphorylation, to initiate sperm motility in salmond fishes.almond fishes.

• Journal of fish pathology
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• v.9 no.2
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• pp.177-183
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• 1996

A new RNA virus isolated from abnormally swimming behavior has caused mortalities in salmonid fish (Oh et al., 1995 a), A reverse transcriptase (RTase) activity of the virus was determined by using poly r(A) : oligo d(T) as templete : primer. This RTase activity was associated with virus particles of buoyant density of 1.16 g/ml. The virus particles in sucrose fractions were enveloped and were about 85 nm diameter with central electron-dense core. The brain and kidney samples of artificially infected fish showed RTase activity. Virus particle associated proteins about 120, 80, 65, 61, 48, 42, 35, 30, 25, 19, and 15 kDa were observed when examined by polyacrylamide gel electrophoresis analysis. This study showed the presence of a new retrovirus in salmonid fish, which tentatively called RVS (Retrovirus of salmonid).

• Annual Conference on Human and Language Technology
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• 1999.10e
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• pp.105-109
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• 1999

영-한 기계번역 중 변환 단계에서 한국어 문장을 생성하기 위해서는 구구조 변환 후 조사 및 대역어 선택으로 이루어진다. 그러나 하나의 영어 단어는 여러 개의 한국어 의미들을 가지고 있기 때문에 문장에서 사용된 영어의 정확한 의미에 해당하는 한국어 대역어를 선택하는 것은 번역의 질을 높이고 시스템의 성능에 매우 중요한 역할을 한다. 특히 용언 및 체언의 대역어 선택은 문장에서 서로 간의 의미적인 관계를 고려하여야 올바른 대역어를 선택할 수 있다. 기존에는 전자 사전에 용언과 체언간의 연어 정보(collocation information)를 구축하여 대역어 선택의 문제를 해결하려고 하였으나 연어 정보가 사전에 존재하지 않을 때 올바른 대역어를 선택할 수 없었다. 또한 용언과 체언의 관계를 나타내는 조사를 선택하기 위하여 격(case)을 세분화하여 사전을 구축하였으나 격의 분류 및 사전을 구축할 경우 격을 선택하는 어려움이 있었다. 이에 따라 본 논문에서는 문형(sentence pattern)에 의한 방법으로 용언의 대역어 및 용언이 갖는 필수격 체언의 조사와 대역어 선택방법을 제안한다. 문형의 구조적인 정보에는 용언과 체언의 의미적 역할(thematic role)을 하는 조사 및 용언이 갖는 필수격 체언의 의미 자질(semantic feature)을 갖고 있다. 이러한 의미 자질을 wordnet과 한/영 및 영/한 사전을 이용하여 의미 지표(semantic marker)를 갖는 문형 사전을 구축한다. 또한 의미 지표를 갖는 문형 사전을 기반으로 조사 및 대역어 선택 알고리즘을 개발한다.

• Journal of the Korean Chemical Society
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• v.29 no.3
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• pp.295-303
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• 1985

Carboxypeptidase B from Salmo Salar eggs was purified by CM-cellulose, 0.5 ammonium sulfate saturation, DEAE-cellulose, and Sephadex G-75 gel filtration and its enzymatic properties were investigated. Optimum temperature was 55$^{\circ}C$, pH optima were 4.0 and 7.0 at 37$^{\circ}C$, and the enzyme was stable at pH 2.0∼3.0 and 5.5∼7.0 for 1.5h. This enzyme showed substrate specificity hydrolyzing the peptide bond of glycyl-L-arginine. Its K$_m$ values was 0.21mM, and the enzyme activity was stimulated by Cu$^{2+}$ and Fe$^{3+}$, while inhibited by Zn$^{2+}$. The lysine was found to be competitive inhibitor and its K$_i$ value was determined to be 4.3mM. Molecular weight of this enzyme was determined to be 36,400 daltons by SDS-PAGE and the enzyme was monomeric protein composed of 19 kinds of amino acid residues.

• Journal of the Korean Chemical Society
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• v.32 no.4
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• pp.377-384
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• 1988

Carotenoprotein from Salmo Salar eggs was purified and characterized by CM-cellulose, 50% $(NH_4)_2SO_4$, DEAE-cellulose and sephadex G-75 column. The chromoprotein had a spectrum with ${\lambda}_{max}$ 409, 540 and 580nm in p-buffer (pH 7.0) at initial step. Molecular weights by sephadex G-200 gel filtration were 50, 200 and 26,000 daltons. SDS-PAGE analysis showed a structure with four identical subunits (12,500 daltons). Its sample retained a small amount of carbohydrates and lipids. Amino acids were analyzed, and mannose, galactose and glucosamine also were identified. Carotenoid extacted with acetone was found to be astaxanthin ester by partition test, epoxy test, iodine test, allylic test, reduction, acetylation, uv/vis, ir and nmr datas. Stearate (47.9%) and palmitate (21.4%) were predominant fatty acids in the astaxanthin ester.