• Polymer(Korea)
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• v.28 no.3
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• pp.218-224
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• 2004

The adhesion and proliferation of mammalian cells on polymeric biomaterials depend on the surface characteristics such as wettability, chemistry, charge and roughness. In order to recognize the correlation between the adhesion and proliferation of human bone marrow derived stem cells (BMSCs) and surface property, radio frequency generated plasma treatment on low density polyethylene (LDPE) has been carried out. The modified LDPE surfaces were characterized by measuring the static water contact angle. The adhesion and proliferation of cells on LDPE films were characterized by cell counting and reverse transcription-polymerase chain reaction (RT-PCR). The water contact angle of the film surface decreased with plasma treatment time. Proto-oncogenes (c-myc, c-fos) and tumor suppressor gene (p153) showed maximum expression with contact angle of 60 ∼ 70$^{\circ}$ range of LDPE film. By cell counting, we confirmed that the rate of cell proliferation appeared the higher on the film surface of the contact angle of 60∼70$^{\circ}$ We concluded that the surface wettability is an important role for the growth and differentiation of BMSCs.

• Journal of Korean Society of Transportation
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• v.30 no.2
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• pp.127-136
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• 2012

Multi-car rear-end collision accidents have three categories: sequential collision from the rear which is commonly referred to as chain reaction collision, sequential collision from the front, and mixed-order collision. This paper suggests several effective methods of reconstruction analysis for multi-car rear-end collision accidents. First, by incorporating the traditional empirical method which uses vehicle damage caused by brake dive and passenger injuries, with results of theoretical analysis made within mechanics of rigid body, it is made possible for the method to be put to immediate practical use. A methodology to precisely analyze multi-car rear-end collision accidents was suggested using a simulation program simultaneously with a video event data recorder which is starting to be widely used in domestic vehicles. To go beyond the simple intuitive analysis of the video event data recorder, the simulation analysis based on the results of video analysis was executed to acquire various information, so that the causes and responsibility could be clearly stated.

• Korean Journal of Microbiology
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• v.38 no.3
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• pp.186-191
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• 2002

The usefulness of oligonucleotide DNA chip was evaluated for detection and primary level identification of major waterborne viruses in environmental samples. The enteric waterborne viruses included enterovirus, adenovirus, and rotavirus. Total intracellular RNA of 10 BGM cell plates showing virus-specific cytopathic effects was extracted at the third day after inoculation. The intracellular RNA was then subjected to either enterovirus-specific RT-PCR followed by sequencing analysis, or the DNA chip. Seven out of 10 positive samples in cell culture were positive but the other three sample were turned out to be negative by both RT-PCR and DNA chip analyses. Nucleotide sequencing results and the DNA chip hybridization results of the RT-PCR product were in complete agreement in the identification of the 7 positive samples as enteroviruses. Using the DNA chip, it took only 3∼4 hr to complete detection and primary level identification of target viruses and additional procedures such as gel electrophoresis or nucleotide sequencing were not necessary. We believe that the DNA chip system can be employed as a highly effective and new detection methodology for environmental viruses.

• Korean Journal of Microbiology
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• v.38 no.3
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• pp.198-204
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• 2002

Poliovirus is a member of enterovirus which causes paralytic poliomyelitis, encephalitis and aseptic meningitis. Since poliovirus is spread by the fecal-oral route and poliovirus-contaminated water could be a potential threat for public health, detection of poliovirus in drinking water resource is important. Infectious poliovirus and poliovirus inactivated by heat or UV were used to test three detection methods such as cell culture method, reverse transcription-polymerase chain reaction (RT-PCR) and integrated cell culture (ICC)-PCR. Infectious poliovirus was detected by all three methods and ICC-PCR was the most sensitive and fast in detecting poliovirus. Inactivated polioviruses could not be detected by cell culture or ICC-PCR methods. On the other hand, heat- inactivated viruses could be detected by RT-PCR. Thus it is suggested that ICC-PCR method is the most sensitive and effective in detecting infectious polioviruses in water sample.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.1
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• pp.83-88
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• 2013

Progressive collapse is a chain reaction of failures propagating throughout a portion of a structure that is disproportionate to the original local failure. When column members are subjected to unexpected load (compression load), they will buckle if the applied load is greater than the critical load that induces buckling. The post-buckling strength of the columns will decrease rapidly, but if there is enough residual strength, the members will absorb the potential energy generated by the impact load to prevent progressive collapse. Thus, it is necessary to identify the relationship of the load-deformation of a column member in the progressive collapse of a structure up to final collapse. In this study, we carried out nonlinear FEM analysis and based on deflection theory, we investigated the load-deformation relationship of H-section steel columns when both ends were fixed.

• Korean Journal of Ecology and Environment
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• v.54 no.3
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• pp.190-198
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• 2021

Human Aichivirus (Aichivirus A; AiV-A) is a positive-sense single-strand RNA non-enveloped virus that has been detected worldwide in various water environments including sewage, river, surface, and ground over the past decade. To develop a method with excellent sensitivity and specificity for AiV-A diagnosis from water environments such as groundwater, a combination capable of reverse transcription (RT)-nested polymerase chain reaction (PCR) was developed based on existing reported and newly designed primers. A selective method was applied to evaluate domestic drinking groundwater samples. Thus, a procedure was devised to select and subsequently identify RT-nested PCR primer sets that can successfully detect and identify AiV-A from groundwater samples. The findings will contribute to developing a better monitoring system to detect AiV-A contamination in water environments such as groundwater.

• Journal of the Korean Orthopaedic Association
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• v.53 no.6
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• pp.552-557
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• 2018

Vitamin D plays a major role in bone metabolism, and its deficiency has an impact on fracture risk and healing. Low vitamin D levels are a cause of poor bone mineralization and have been associated with a significantly higher risk of physeal injury in children. This paper presents a case of a 13-year-old boy with a vitamin D deficiency, who sustained multiple sequential epiphyseal injuries at various areas. This report suggests that vitamin D deficiency is not only a significant cause of the clinical disease itself, but also an important factor affecting the successful recovery of injuries.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.34 no.2
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• pp.19-28
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• 2018

The progressive collapse resistance performance of a steel structure constructed using the moment frame with the WUF-B connection and the composite slabs was evaluated. GSA 2003 was adapted for the evaluation. Additionally the structural robustness and the sensitivity against the progressive collapse were analyzed. In the numerical analysis, a reduced model comprised of the beam and spring elements for WUF-B connection was adapted. The composite slab was modeled using the composite-shell element. Instead of the time-consuming dynamic analysis for the effect of the sudden column removal, the equivalent energy-based static analysis was effectively applied. The analysis results showed that the structure was the most vulnerable to in the case of the internal column removal, however it satisfied the chord rotation criterion of GSA 2003 due to the contribution of the composite slab which improved the stiffness of structure. In the robustness evaluation, the structural performance showed more than 2.5 times of the requirement according to GSA 2003, and the structural sensitivity analysis indicated the decrease of 33% of the initial structural performance.

• Journal of Korean Society of Forest Science
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• v.109 no.4
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• pp.521-531
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• 2020

In order to identify the economic impacts of forest-related industry on the national economy in general and the linkages between forestry and its related industries, an input-output analysis was conducted using the 2015 Input-Output Tables of the Bank of Korea. Production inducement effects are relatively high in paper products, lumber, and silviculture and relatively low in other wood industries. Value-added inducement effects are relatively high in silviculture and log production. With respect to the wood products industry, forward linkage effects are higher and backward linkage effects are lower. A Ritz-Spaulding multiplier analysis revealed that the growth of wood products production has only a modest impact on the growth of forestry. How to increase the linkage between forestry and the wood products industry is one of the most important policy tasks in Korea, given that it affects forest ecosystem management and climate change mitigation efforts.

• Journal of Apiculture
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• v.32 no.2
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• pp.99-109
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• 2017

The multiple real-time PCRs against pathogens of Bombus species including DWV, IAPV, KBV, SBV, BQCV, kSBV, SBPV and Paenibacillus larvae, Mellisococcus plutonius, Lysinibacillus fusiformis, and Klebsiella oxytoca have been developed. One extracted nucleic acid from Beesample could be applied to 11 different PCRs in same time and condition. Specific PCR-products were amplified qualitative and quantitative manner inner 20 minutes successfully, when each 1000 molecules of pathogen-specific target DNA is existed as template, respectively. The multiple PCR detection that we propose would be expected to apply to quarantine test for international exchange of Bombus species.