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Detection of Poliovirus in Water by Cell Culture and PCR Methods  

조연희 (충북대학교 자연과학대학 생명과학부)
이찬희 (충북대학교 자연과학대학 생명과학부, 충북대학교 바이오연구소)
Publication Information
Korean Journal of Microbiology / v.38, no.3, 2002 , pp. 198-204 More about this Journal
Abstract
Poliovirus is a member of enterovirus which causes paralytic poliomyelitis, encephalitis and aseptic meningitis. Since poliovirus is spread by the fecal-oral route and poliovirus-contaminated water could be a potential threat for public health, detection of poliovirus in drinking water resource is important. Infectious poliovirus and poliovirus inactivated by heat or UV were used to test three detection methods such as cell culture method, reverse transcription-polymerase chain reaction (RT-PCR) and integrated cell culture (ICC)-PCR. Infectious poliovirus was detected by all three methods and ICC-PCR was the most sensitive and fast in detecting poliovirus. Inactivated polioviruses could not be detected by cell culture or ICC-PCR methods. On the other hand, heat- inactivated viruses could be detected by RT-PCR. Thus it is suggested that ICC-PCR method is the most sensitive and effective in detecting infectious polioviruses in water sample.
Keywords
cell culture; ICC-PCR; PCR; poliovirus; water;
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Times Cited By KSCI : 2  (Citation Analysis)
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