• Journal of Life Science
• /
• v.33 no.3
• /
• pp.295-303
• /
• 2023

Immune and metabolic systems are important factors in maintaining homeostasis. Immune response and metabolic regulation are highly associated, so, when the normal metabolism is disturbed, the immune response changed followed the metabolic diseases occur. Likewise, obesity is highly related to immune response. Obesity, which is caused by an imbalance in energy metabolism, is associated with metabolic diseases, such as insulin resistance, type 2 diabetes, fatty liver diseases, atherosclerosis and hypertension. As known, obesity is characterized in chronic low-grade inflammation. In obesity, the microenvironment of immune cells became inflammatory by the unique activation phenotypes of immune cells such as macrophage, natural killer cell, T cell. Also, the immune cells interact each other in cellular or cytokine mechanisms, which intensify the obesity-induced inflammatory response. This phenomenon suggests the possibility of regulating the activation of immune cells as a pharmacological therapeutic strategy for obesity in addition to the common pharmacological treatment of obesity which is aimed at inhibiting enzymes such as pancreatic lipase and α-amylase or inhibiting differentiation of preadipocytes. In this review, we summarize the activation phenotypes of macrophage, natural killer cell and T cell, and their aspects in obesity. We also summarize the pharmacological substances that alleviates obesity by regulating the activation of immune cells.

• Tuberculosis and Respiratory Diseases
• /
• v.56 no.2
• /
• pp.187-197
• /
• 2004

Background : Nitric Oxide (NO) is a multi-faceted molecule with dichotomous regulatory roles in many areas of biology. NO can promote apoptosis in some cells, whereas it inhibits apoptosis in other cell types. This study was performed to characterize NO-induced cell death in lung epithelial cells and to investigate the roles of cell death regulators including iron, bcl-2 and p53. Methods : A549 cells were used for lung epithelial cells. SNP (sodium nitroprusside) and SNAP (S-nitroso-N-acetyl- penicillamine) were used for NO donor. Cytoxicity assay was done by MTT assay and crystal violet assay. Apoptotic assay was done by fluorescent microscopy after double staining with propidium iodide and hoecst 33342. Iron inhibition study was done with RBCs and FeSO4. For bcl-2 study, bcl-2 overexpressing cells (A549-bcl-2) were used and for p53 study, Western blot analysis and p53 functionally knock-out cells (A549-E6) were used. Results : SNP and SNAP induced dose-dependent cell death in A549 cells and fluorescent microscopy revealed that SNAP induced apoptosis in low doses but necrosis in high doses while SNP induced exclusively necrotic cell death. Iron inhibition study using RBCs and FeSO4 significantly blocked SNAP-induced cell death. And also SNAP-induced cell death was blocked by bcl-2 overexpression. Finally, we found that SNAP activate p53 by Western blot analysis and that SNAP-induced cell death was decreased in the abscence of p53. Conclusion : In lung epithelial cells, NO can induce cell death, more precisely apoptosis in low doses and necrosis in high doses. And iron, bcl-2, and p53 play important roles in NO-induced cell death.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
• /
• 2005.11a
• /
• pp.73-78
• /
• 2005

본 연구에서는 서울시 한강에 위치한 교량을 대상으로 다양한 조망점에 따른 경관조명의 물리량을 측정하고 그 특성을 분석함으로써 동일한 연구대상에 대하여 조망점이 달라졌을 때 나타나는 물리적 변화를 통해 경관조명분야의 물리량평가방법론의 새로운 접근방법론을 제시하는 것이 목적이다. 이를 위하여 연구대상의 선정은 교량구조형식에 따라 분류하고. 경관조명이 설치된 교량 17개소 중 다양한 조망점과 현장측정의 편리성 등을 고려하여 광진교(거더교), 올림픽대교(사장교), 동작대교(트러스교), 동작대교(아치교)를 연구대상으로 선정하였다. 조망점은 한강둔치를 이용하는 방문객들이 바라보는 투시뷰, 교량주변 건축물에서 바라보는 건물뷰 그리고 교량 위를 이동하면서 바라보는 주행뷰로 구분하였다. 이러한 연구방법으로 도출된 결과는 동일한 경관조명연출을 토대로 교량별 조망점에 따른 휘도측정 및 분석에서 측정되는 각도, 거리, 방향에 따라 정형화된 비율은 아니지만 휘도값에 있어서 차이가 있다는 것을 확인 할 수 있었으며, 투시뷰와 건물뷰는 동일한 교량 구성요소를 갖고 있어 상호간 비교 분석이 가능하고 경관조명평가 요소로서 그 기능을 담당 할 수 있다고 판단되며, 주행뷰는 교량형식과 가로등의 영향을 많이 받으므로 평가요소로써 조망점을 활용하기에는 조금 더 깊은 연구가 선행되어야 할 것으로 보인다. 따라서 경관조명을 평가함에 있어 다양하게 바라보는 조망점은 조명연출로 인한 시각적 인식에 차이를 유발시킬 수 있어 경관조명을 평가할 때 다양한 조망점의 설정이 보다 객관적이고 정확한 분석이 가능할 것으로 판단된다.ridinium의 분포는 chlorophyll-a농도와 밀접한 관련성이 있었다. 담수적조의 현존량은 상류지역에서 $8.5\;{\times}\;10^3\;cell\;mL^L{-1}$로서 많았고, 댐부근의 하류지역($4.4\;{\times}\;10^2\;cell\;mL^L{-1}$)으로 갈수록 감소하는 양상을 보였다. 또한, 담수적조의 원인종으로 규조류 Synedra acus와 남조류 Microcystis aeruginosa도 소량관찰되었다. 저수지의 수질 부영양화 현상은 기존 경사사면형 농경지를 기반으로 조성되었으므로 저층으로부터 풍부한 영양환경을 내재하고 있었고, 담수 초기에 발생할 수 있는 가능성을 포함하고 있었다. 또한 유입수량의 부족과 현재 수질개선을 위해 적용하고 있는 수중폭기시설등의 물리적인 영향도 직 ${\cdot}$ 간접적으로 작용하였을 것으로 추정되었다 따라서 향후 이에 대한 중장기적인 모니터링이 필요하였고 유역과 저수지의 통합적인 수질관리계획 이 요구되었다.group으로, 용존산소량, 전기전도도, pH, 인산염이 정점 3, 4, 5에서 같은 group으로 묶였다.유의적인 CAT 활성 증가효과가 나타났고, 간조직의 시토졸 1획분에서는 대조군 대비 15${\sim}$27%의 CAT 활성 증가효과가 인정되었다. 이상의 결과에서 목초액의 장기간 투여는 간조직 중의 활성산소의 억제효과뿐만 아니라 방어시스템으로서 활성산소 제거효소의 역할도 충실히 수행하여 노화를 효과적으로 예방하고 억제할 수 있을 것으로 기

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.33 no.1
• /
• pp.66-69
• /
• 2000

The common harpacticoida copepod species Tigriopus japnnicus was often obseued in the mass culture of marine rotifer tanks of sea farming stations. In this study, we investigated the co-existing effect of T. iaponicus to the rotifer Brachionus rotundiformis population growth. The culture conditions as temperature, salinity, culture volume, photo period, culture period and observation interval were 25{\circ}C, 22ppt, 40ml, 12L:12D, 16 deys$ and every two days during the experimental period, respectively. Nannochloropsis oculata was used as the food for the two testing organisms. After counting the growth of rotifer and copepod, they were transferred to new culture tank with N. oculata at the density of $7{times}10^5\;cells/ml$. The population growth of B. rotundiformis was suppressed by T. japonicus when these two organisms were mix-cultured comparing to single culture of B. rotundiformis. But population growth of T, japomcus was not affected by the presence of B. rotundiformis. The interspecific relationships of prey-predation and food competition were not observed in the combination culture of rotifer B. rotundiformis and copepod T japonicus.

• Journal of Applied Biological Chemistry
• /
• v.52 no.3
• /
• pp.103-108
• /
• 2009

Little is known about roles of adipose tissue, although obesity is one of the potential risk factors in causing breast cancer and adipose tissue surrounding breast ductal cells is the largest organ. To identify the genes that are regulated by factors secreted from adipocytes in breast cancer cells, MDA-MB-231 cells were treated with the culture medium of adipocytes. In present study glucocorticoid-induced TNF receptor-related protein ligand (GITRL) gene was studied among the induced genes. It was found that GITRL was significantly increased by the culture medium of adipocytes. Proteinase K-treated adipocyte culture supernatants failed to induce GITRL expression. These findings indicate that unknown protein factors are responsible for the induction of GITRL expression. The expression of GITRL did not affect the invasive ability of MDA-MB-231 cells, but coculture with NK92 expressing GITR suppressed the invasiveness of MDA-MB-231 cells.

• Proceedings of the Korean Information Science Society Conference
• /
• 2005.11a
• /
• pp.19-21
• /
• 2005

현대의 산업화 사회에서는 자동인식을 통해서 사람과 사물을 식별하고자 하는 연구들이 진행되고 있다. 그 대표적인 예로 바코드를 이용한 접촉식 판별기술이 있고, 라디오 주파수를 이용한 RFID(Radio Frequency Identification) 기술을 들 수 있다. RFID의 경우는 무선 주파수를 이용하기 때문에 대량의 사물을 동시에 인식 할 수 있다는 장점이 있다. 하지만. 어떠한 상황에서 리더의 요청에 응답을 하는 리더-태그 시스템이기 때문에 사용자의 프라이버시 침해 문제를 야기 할 수 있다. 사용자의 프라이버시 침해문제를 막기 위해서 많은 연구들이 진행되고 있다. 그 중에서, Miyako Ohkubo의 Hash체인을 이용한 프라이버시 보호 기법은 정보유출, 위치추적공격(Location Tracking Attack), 전방위보안성(Forward Security)과 같은 프라이버시 침해문제들로부터 사용자의 프라이버시를 보호 할 수 있는 프로토콜이다. 그러나 Hash함수를 태그에 구현하는 것은 현재까지는 불가능한 상황이다. 또, Martin Feldhofer의 AES(Advanced Encryption Standard)를 사용한 프로토콜은 실제로 태그에 구현 가능하면서 내부구조가 8bit인 AES를 사용함으로써 암호학적인 강도를 높였으나, 프라이버시 침해 문제에서 단점을 드러냈다. 이러한 단점을 보완한 AES기반에서의 개선된 RFID 프라이버시 보호 프로토콜은 실제적으로 태그에 구현 가능한 AES를 이용한 암호화 체인을 통해서 프라이버시 보호에 우수하면서 실제 사용이 가능한 프로토콜을 제안하였다[1]. 그러나, 이 프로토콜은 생성되는 키 값들이 물리적 공격을 통해서 노출이 되었을 때, 이전의 seed값과 키 값들이 노출 되는 단점이 있다. 본 논문에서는 이러한 문제들을 해결하고자 프라이버시보호에 새로운 키 생성 방법을 통한 강력한 프로토콜을 제안 한다.하였으나 사료효율은 증진시켰으며, 후자(사양, 사료)와의 상호작용은 나타나지 않았다. 이상의 결과는 거세비육돈에서 1) androgen과 estrogen은 공히 자발적인 사료섭취와 등지방 침적을 억제하고 IGF-I 분비를 증가시키며, 2) 성선스테로이드호르몬의 이 같은 성장에 미치는 효과의 일부는 IGF-I을 통해 매개될 수도 있을을 시사한다. 약 $70 {\~} 90\%$의 phenoxyethanol이 유상에 존재하였다. 또한, 미생물에 대한 항균력도 phenoxyethanol이 수상에 많이 존재할수록 증가하는 경향을 나타내었다. 따라서, 제형 내 oil tomposition을 변화시킴으로써 phenoxyethanol의 사용량을 줄일 수 있을 뿐만 아니라, 피부 투과를 감소시켜 보다 피부 자극이 적은 저자극 방부시스템 개발이 가능하리라 보여 진다. 첨가하여 제조한 curd yoghurt는 저장성과 관능적인 면에서 우수한 상품적 가치가 인정되는 새로운 기능성 신제품의 개발에 기여할 수 있을 것으로 사료되었다. 여자의 경우 0.8이상이 되어서 심혈관계 질환의 위험 범위에 속하는 수준이었다. 삼두근의 두겹 두께는 남녀 각각 $20.2\pm8.58cm,\;22.2\pm4.40mm$으로 남녀간에 유의한 차이는 없었다. 조사대상자의 식습관 상태는 전체 대상자의 $84.4\%$가 대부분이 하루 세끼 식사를 규칙적으로 하고 있었으며 식사속도는 허겁지겁 빨리 섭취하는 경우가 남자는 $31.0\%$, 여자는 $21.4\%$로 나타났고 이들을 제외한 나머지 사람들은 보통 속도 혹은 충분한 시간을 가지고 식사를 하였다. 평소 식사량은 조금 적게 혹은 적당하게 섭취하는 사람이 대부분이었으며 남자가 여자보다는 배부르게 먹는 경 향이 유의적으로 높았다(p<0.05). 식사는 혼자 하는 경우가 남자

• Journal of Life Science
• /
• v.30 no.5
• /
• pp.483-490
• /
• 2020

The ubiquitin system uses ligases and deubiquitinases (DUBs) to regulate ubiquitin position on protein substrates and is involved in many biological processes which determine stability, activity, and interaction of the target substrate. DUBs are classified in six groups according to catalytic domain, namely ubiquitin-specific proteases (USPs); ubiquitin C-terminal hydrolases (UCHs); ovarian tumor proteases (OTUs); Machado Joseph Disease proteases (MJDs); motif interacting with Ub (MIU)-containing novel DUB family (MINDY); and Jab1/MPN/MOV34 metalloenzymes (JAMMs). Otubain 1 (OTUB1) is a DUB in the OTU family which possesses both canonical and non-canonical activity and can regulate multiple cellular signaling pathways. In this review, we describe the function of OTUB1 through regulation of its canonical and non-canonical activities in multiple specifically cancer-associated pathways. The canonical activity of OTUB1 inhibits protein ubiquitination by cleaving Lys48 linkages while its non-canonical activity prevents ubiquitin transfer onto target proteins through binding to E2-conjugating enzymes, resulting in the induction of protein deubiquitination. OTUB1 can therefore canonically and non-canonically promote tumor cell proliferation, invasion, and drug resistance through regulating FOXM1, ERα, KRAS, p53, and mTORC1. Moreover, clinical research has demonstrated that OTUB1 overexpresses with high metastasis in many tumor types including breast, ovarian, esophageal squamous, and glioma. Therefore, OTUB1 has been suggested as a diagnosis marker and potential therapeutic target for oncotherapy.

• Journal of Life Science
• /
• v.28 no.12
• /
• pp.1432-1437
• /
• 2018

Clara cell secretory protein (CCSP) plays an important role in protecting the lungs from inflammation. This research focuses on identifying the cis-element for binding the repressor of CCSP gene expression. A DNase I footprinting experiment revealed three protected regions between -812 and -768 bp (45 bp) of the mCCSP promoter. One motif (D3: GCCTGGGAA) was 100% conserved across rat, hamster, and human. The addition of excess amounts of the D3 motif exhibited high competition within that 45 bp range in an electrophoretic mobility shift assay. However, when mutated D3 ($G{\underline{AA}}TG{\underline{TT}}AA$) was used, the competition was significantly reduced. This demonstrates that the D3 motif within that 45 bp region of the mCCSP promoter is an important site for the protein-DNA interaction. Transient transfection assays with -756 Luc resulted in highly decreased expression of CCSP than those with -812 Luc, suggesting that the 45 bp could function as a binding site for the repressor. Co-transfection of KLF4 exhibited significant repression of the -812 Luc but not the -768 Luc which clearly shows that KLF4 might function as a repressor for the CCSP gene and also suggests that the D3 motif is strongly involved in the binding of KLF4. In addition, when anti-KLF4 antibody was added, super-shifted bands were observed. This result demonstrates that KLF4 could function as a repressor by binding to this 45 bp region of the CCSP promoter and that the D3 motif might be involved in the specific binding of KLF4.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.6
• /
• pp.714-720
• /
• 2008

Cordyceps militaris is a medicinal fungus which has been used for patient suffering from cancer in Oriental medicine. It was previously reported that C. militaris extracts are capable of inhibiting tumor growth and inducing apoptosis; however, the anti-poliferative effects of human cancer cells have been poorly understood. In this study, to elucidate the anti-cancer mechanisms of human cancer cells by treatment with aqueous extract of C. militaris (AECM), we investigated the anti-proliferative effects of AECM in human hepatocarcinoma Hep3B cells. AECM treatment inhibited the growth of Hep3B cells and induced the apoptotic cell death in a concentration-dependent manner such as formation of apoptotic bodies and increased populations of apoptotic-sub G1 phase. The induction of apoptosis by AECM was connected with a proteolytic activation of caspase-3 and caspase-8. and concomitant degradation of poly (ADP-ribose) polymerase (PARP) and ${\beta}$-catenin proteins. Furthermore, caspase-3 inhibitor, z-DEVD-fmk, significantly inhibited AECM-induced apoptosis demonstrating the important role of caspase-3 in the bserved cytotoxic effect. Taken together, these findings suggest that AECM-induced inhibition of human hepatocarcinoma cell proliferation is associated with the induction of apoptotic cell death via activation of caspase-3 and C. militaris may have therapeutic potential in human cancer.

• Membrane Journal
• /
• v.12 no.4
• /
• pp.207-215
• /
• 2002

The hindered diffusion in confined spaces is an important phenomenon to understand in a micro-scale the filtration mechanism determined by the particle motion in membrane pores. Compared to the case of spherical colloids, both the theoretical investigations and the experiments on the hindered diffusion of polyelectrolytes is actually more difficult, due to lots of relevant parameters resulting from the complicated conformational properties of the polyelectrolyte chain. We have successfully performed the Brownian dynamics simulations upon a single polyeiectrolyte confined in a slit-like pore, where a coarse-grained bead-spring model incorporated with Debye-Huckel interaction is properly adopted. For the given sizes of both the polyelectrolyte and the pore width, the hindered diffusion coefficient decreases as the solution ionic concentration decreases. It is evident that a charge effect of the pore wall enhances the hindered diffusion of polyelectrolyte. Simulation results allow us to make sense of the diffusive transport through the micro-pore, which is restricted by the influences of the steric hindrance of polyelectrolytes as well as the electrostatic repulsion between the polyelectrolytes and pore wall.