• Title/Summary/Keyword: 어패류

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Characteristics and Antibiotics Susceptibility of Escherichia coli Isolated from Fishery Products (수산물에서 분리한 대장균의 분리특성 및 항생제 감수성)

  • 이재인;한기영;박홍현
    • The Korean Journal of Food And Nutrition
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    • v.16 no.2
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    • pp.111-115
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    • 2003
  • This study was practiced to investigate characteristics and antibiotics susceptibility to 296 isolates of Escherichia coli isolated from 2429 fishery samples sold in Noryangjin Fishery Market from Sep. 2001 to Aug. 2002 It showed 12.2% average E. coli isolation rates for the tested samples. Isolation rates were high in summer, especially July and August, but there was no isolate in winter season. Of the fishery groups, the isolation rate showed in crustacea(15.6%), in mollusca(14.4%), in shellfish(12.0%) and in fish(11.0%). But, differences between the fishery groups was not large. In the case of shellfish, the isolation rate of shucked shellfish (16.3%) was higher than the non-shucked shellfish (6.8%). Following the provinces of origin of the fishery samples, in Gyung-Nam (14.3%) showed the highest isolation rate, whereas Je-Ju (6.9%) showed the lowest isolation rate. Results in 12 kinds of antibiotics susceptibility test, the 169 isolates(57.1%) had antibiotics resistances at least one kind of antibiotics and the 104 isolates (35.1%) had the multiple resistance at least two kinds.

A New Analytical Method for Fluoroquinolones in Fisheries Products by High Performance Liquid Chromatography (HPLC를 이용한 어패류 중의 fluoroquinolone계 항균제의 분석법)

  • Jo Mi-Ra;Kim Poong-Ho;Lee Hee-Jung;Lee Tae-Seek
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.39 no.2
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    • pp.59-65
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    • 2006
  • Fluoroquinolones are the most common group of antibacterial agents currently used in the Korean aquaculture industry, and use of these agents has been increasing steadily. High performance liquid chromatography (HPLC) with fluorescence detection was used for the simultaneous determination of five fluoroquinolones in fish and shellfish: ofloxacin (OFL), pefloxacin (PEF), norfloxacin (NOR), ciprofloxacin (CIP), and enrofloxacin (ENRO). Fish and shellfish muscle was homogenized, and protein, lipid, and low molecular weight pigments were then excluded from the homogenate. The final eluates were analyzed by HPLC equipped with a Shiseido UG-120 type C18 reverse-phase column ($4.6{\times}250 mm$, $5{\mu}m$) and a fluorescence detector (excitation at 280 nm, emission at 450 nm). The mobile phase was 0.1 M phosphoric acid and acetonitrile solution (91:9, v/v) and tetrahydrofuran (THF) was added to it at a rate of 5 mL per a liter of the mobile phase. Adequate chromatography separation was obtained using the above method. Average recoveries of fortified samples at levels from 0.05 to 0.5 mg/kg were $72.3{\pm}2.5-84.5{\pm}1.2%$ for OFL, $82.7{\pm}3.3- 109.3{\pm}7.5%$ for NOR, $85.3{\pm}6.6-116.0{\pm}7.9%$ for PEF, $76.0{\pm}4.3-109.3{\pm}12.4%$ for CIP, and $78.7{\pm}5.9-100.0{\pm}9.8%$ for ENRO. The limit of detection of OFL was $5{\mu}g/L$, the others were $1{\mu}g/L$. We concluded that the new analytical method was suitable for the determination of fluoroquinolones in fish and shellfish.

Assessment (If Estimated Daily Intakes of N-Nitrosamine by Diet (식사를 통한 N-Nitrosamine의 추정 섭취량 평가)

  • 성낙주;신정혜;김연희;이수정;손미예
    • The Korean Journal of Food And Nutrition
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    • v.15 no.1
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    • pp.29-35
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    • 2002
  • N-nitrosamine(NA) contents depending on simulated gastric digestion were analyzed with 12 kinds of diets collected from institutional food service those diets were estimated the total NA amounts including both intake from food directly and its endogenous formation in human body from simulated gastric digestion. NA was determined in dishes of meats, fishes and vegetables before and after simulated gastric digestion. Before digestion, N-nitrosodimethylamine (NDMA) contents ware from not detected(ND) to 4.8$\mu\textrm{g}$/kg in dishes of meats and fishes. After digestion, its contents increased and the highest level was 3.0 $\mu\textrm{g}$/kg in panbroiled dried anchovy. In vegetable dishes, NDMA was detected as ND∼trace before and after digestion. The contents of NDMA in diets collected from institutional food service were 0.20∼0.78$\mu\textrm{g}$/kg 0.43 ∼ 0.80$\mu\textrm{g}$/kg before and after digestion, respectively. The average intake of NA per day to Korean, based on the above data, was 0.60 ∼ 2.34$\mu\textrm{g}$/day/ person. The maximum daily intake of NA was deduced to 5.15$\mu\textrm{g}$/day/person when considering NA amounts formed endogenously by simulated gastric digestion.

Hydraulic model test for corrugated artificial reef stability (수리실험을 통한 요철형 인공어초 안정성 검토)

  • Baek, Seung Hwa;Shin, Bum-Shick;Kim, Kyu-Han
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.8
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    • pp.5327-5332
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    • 2014
  • In Korea, the quantitative growth rate of artificial reef construction is supposed to reach the peak point. Therefore, new approach is needed to the point of artificial reefs business. Functional reefs, such as shellfish reefs, recreational reefs, seaweed reefs, and fish reefs, are beneficial alternatives. This study conducted hydraulic testing to assess the stability of corrugated artificial reefs (ARs) that were constructed to promote the growth of shellfish and seaweed. The results of this study showed that some dimensionless design parameters affected the stability of corrugated artificial reefs under a range of wave and water depth conditions in a fixed bed condition. The findings also highlight the importance of hydraulic experiments in solving the problems that have emerged in the design and construction of artificial reefs.

Analysis of Tetracyclines Using Righ-Perforklance Liquid Chromatography for Fishery Products (HPLC를 이용한 어패류 중의 테트라사이클린계 항생제 분석방법 개발)

  • LEE Hee Jung;LEE Tae Seek;SON Kwang Tae;KIM Poong Ho;JO Mi Ra;PARK Mi Jung;Yi Young Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.6
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    • pp.372-378
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    • 2005
  • An analysis method of tetracyclines far fish and shellfish products containing large amount of low molecular materials and pigments was established. The recovery of the established analysis method for four tetracycline samples was $72-100\%$ and higher than other methods reported. Especially, proposed sample treatment protocol was shown to be effective for the removal of low molecular materials and pigments that tend to interfere with accurate analysis. The detection limit of oxytetracycline (OTC) and tetracycline (TC) from the sample was 0.02 ppm, and the detection limit of chlortetracycline (CTC) and doxycycline (DC) from the sample was 0.1ppm, To examine the efficiency of the established method and identify tetracycline usage in fish farms, tetracycline group antibiotics in the flounder being cultured was monitored. The improved method can be used for fish and shellfish products effectively and all surveyed fish farms have used tetracycline all the year round. The proposed method was adopted as official method for fishery products by Korean Food and Drug Administration in 2003 and it is being used by regulatory authority as National Fishery Products Quality Inspection Service.

THE CONCENTRATIONS OF MERCURY, CADMIUM, LEAD AND COPPER IN FISH AND SHELLFISH OF KOREA (한국산어패류중의 수은 카드뮴 납 구리의 함량)

  • WON JongHeun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.6 no.1_2
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    • pp.1-19
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    • 1973
  • Heavy metal concentrations have been determined in fishes and shellfishes sampled from October 1971 to April 1972 in Korea. In general, fish viscera, shellfish muscle and crustacean exoskeleton contained high concentrations of the metals. With regard to the regional variations of the metals, samples of the west coast of Korea contained relatively high concentrations of mercury, copper, cadmium and lead, but those of the south coast contained high levels of cadmium and lead. In particular, the concentrations of copper in the samples of the west coast were almost twice as much those of the other coasts. The concentrations of mercury in the samples, as a whole, ranged from 0.02 to 0.58 ppm with an average of 0.16 ppm. The concentrations of cadmium were higher in the fish viscera and shellfish than the fish muscle, ranging from less than 0.02 to 0.78 ppm with an average of 0.12 ppm in the fish and shellfish muscle samples. The lead concentrations were slightly high in the fish viscera. In the muscle and bone of fish and shellfish, the range was 0.06-4.84 ppm with an average of about 1 ppm. The concentrations of copper were very high in the viscera of fish and shellfish. The shellfish muscle contained almost four times as much copper as in the fish muscle, particularly cephalopods, i. e., squid and octopus, contained very high levels of copper, and oysters collected from Gunsan area on the west coast contained the highest levels of copper. The concentrations of copper ranged from 0.12 to 28.7 ppm with an average of 1.4 ppm in the fish muscle and of 5.9 ppm in the shellfish muscle.

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Vibrio parahaemolyticus IN KOREAN COASTAL WATERS 3. The Ditribution of Vibrio Flora in the Public Beach of Busan City (한국연안의 호염성 비브리오균에 관한 연구 3. 부산시 해수욕장의 호염성 비브리오균의 분포)

  • LEE Won-Jae;CHOF Wi-Kyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.6 no.1_2
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    • pp.20-26
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    • 1973
  • Authors have investigated the monthly distribution of vibrio flora in sea water, mud, fish and shellfish in the public beaches at the suburbs of Busan in order to determine the possible origins of food poisoning. The results are summarized as follows: 1. The monthly distribution shows that V. parahaemolyticus occurs from May to November and V. alginelyticus all the year round. 2. A total of 40 strains of Vibrio flora were isolated from 40 sea water samples, 25 strains from 40 mud samples, If strains from 88 shellfish samples, and 3 strains from 30 fish samples. 3. More strains were isolated from the samples collected at Yongho and Suyeong that at the other localities. 4. A total of 80 strains of Vibrio flora, isolated from 198 samples, were divided into two groups : V. perahaemolyticus with 20 strains and V. alginelyticus 60 strains.

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Enhancing of Red Tide Blooms Prediction using Ensemble Train (앙상블 학습을 이용한 적조 발생 예측의 성능향상)

  • Park, Sun;Jeong, Min-A;Lee, Seong-Ro
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.49 no.1
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    • pp.41-48
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    • 2012
  • Red tide is a natural phenomenon temporary blooming harmful algal with changing sea color from normal to red, which fish and shellfish die en masse. It also give a bad influence to coastal environment and sea ecosystem. The damage of sea farming by a red tide has been occurred each year which it cost much to prevent disasters of red tide blooms. Red tide damage and prevention cost of red tide disasters can be minimized by means of prediction of red tide blooms. In this paper, we proposed the red tide blooms prediction method using ensemble train. The proposed method use the bagging and boosting ensemble train methods for enhancing red tide prediction and forecast. The experimental results demonstrate that the proposed method achieves a better red tide prediction performance than other single classifiers.

Analysis of Vitamin E in Some Commonly Consumed Foods in Korea (국내에서 소비되는 일부 상용 식품의 비타민 E함량 분석)

  • Lee, Seon-Mi;Lee, Hee-Bong;Lee, Junsoo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.7
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    • pp.1064-1070
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    • 2005
  • Tocopherol and tocotrienol contents of commonly consumed foods in Korea were determined by saponification or direct solvent extraction followed by normal phase liquid chromatography. All samples were locally obtained in the year of 2002 and 2003. The study included 13 meats, 15 fishes and shellfishes, 4 seaweeds, 7 mushrooms, 19 milk and milk products, 6 legumes, 12 nuts, 17 processed foods and 17 Korean traditional foods. All of the vitamin E isomers were quantitated and the results were expressed as $\alpha-tocopherol$ equivalent $(\alpha-TE)$. The relatively higher amount of vitamin E was found in beef boiled in soy, dried squid, toasted seaweed, milk powder, soritae, sunflower seeds, ramyon (instant noodle), and kochujang from meats, fishes, seaweeds, milk products, legumes, processed foods, and traditional foods, respectively. This study provided reliable vitamin E data in commonly consumed foods in Korea for the nutritional information and food composition database.

Use of a Drone for Mapping and Time Series Image Acquisition of Tidal Zones (드론을 활용한 갯벌 지형 및 시계열 정보의 획득)

  • Oh, Jaehong;Kim, Duk-jin;Lee, Hyoseong
    • Journal of the Korean Institute of Intelligent Systems
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    • v.27 no.2
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    • pp.119-125
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    • 2017
  • The mud flat in Korea is the geographical feature generated from the sediment of rivers of Korea and China and it is the important topography for pollution purification and fishing industry. The mud flat is difficult to access such that it requires the aerial survey for the high-resolution spatial information of the area. In this study we used drones instead of the conventional aerial and remote sensing approaches which have shortcomings of costs and revisit times. We carried out GPS-based control point survey, temporal image acquisition using drones, bundle adjustment, stereo image processing for DSM and ortho photo generation, followed by co-registration between the spatio-temporal information.