• Journal of Environmental Impact Assessment
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• v.24 no.4
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• pp.344-351
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• 2015

In this study, bio-electrokinetics was used to increase migration of arsenic by activating endemic microorganisms in the soil. In this technology, bio-electrokinetics which the cultured soil microorganisms and nutrients injected combines with biological technology. This technology using electrical movement of microorganisms could overcome the weakness of late degradation speed and low removal efficiency. And, various soil microorganisms reduce ferreous, manganese, etc., using organic matter by as an electron donor by injecting mixture of soil microorganisms and nutrients instead of using electrolyte of the electrode. Accordingly, surrounding metal oxide microorganisms convert arsenic (III) to arsenic (V) to increase migration of arsenic (III), in consequence, migration of arsenic increased in 60 to 70% compared to about 30% of conventional electrokinetics.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.491-496
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• 2000

A RAG25 gene regulating flowering time was introduced into Codonopsis lanceolata through high efficiencies (ca. 90%) of plant regeneration. The leaf explants were immersed in YEP media containing Agrobacterium tumefaciens (pGA 1209) harboring RAG25 gene, and cocultivated for 3 days. After cocultivation, they were cultured in shoot inducing media (SIM), N2B2 (NAA 2 mg/L, BA 2 mg/L and kanamycin 20 mg/L) and N2B4 (NAA 2 mg/L, BA 4 mg/L and kanamycin 20 mg/L), and the putative transformants were regenerated. The introduction of nptII and RAG25 gene into Codonopsis lanceolata was confirmed by 0.7 kb and 0.6 kb bands from polymerase chain reaction and reconfirmed by Southern hybridization using PCR product of RAG25 gene.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.6
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• pp.833-838
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• 1993

A strain of Penicillium sp. extracellularly produced an inhibitory substance for lipoxygenase. These purification procedures were followed : ethanol treatment, chromatographies on Dowex 50W, Sephadex G-25, silica gel column and HPLC. The inhibitor was stable in pH range from 3.0 to 5.0 at $25^{\circ}C$, and a treatment at 10$0^{\circ}C$ for 2 hours didn't diminish its original activity. The purified inhibitor was charred at temperature near 22$0^{\circ}C$~23$0^{\circ}C$ and decomposed. Molecular weight of the inhibitor was estimated to be approximately 270 by Sephadex G-25 column chromatography. The inhibitor rapidly formed EI complex with lipoxygenase and inhibited enzyme activity.

• KSBB Journal
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• v.6 no.1
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• pp.27-33
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• 1991

Hairy root induced by A. rhizogenes from potato tuber (Solanm tuberosum L.) synthesized the agropine and mannopine which were demonstrated with paper electrophoresis. And the starch contents in hairy root were increased gradually following the developmental stage. But protein contents were decreased. The activity of ${\beta}-glucan$ synthetase II(GSII) which is related to the cell wall biosynthesis was stimulated in hairy root on the developmental stage. And chloropromazine did not influence the activity of GS II while verapamil inhibited about 60% of the activity GS II. Therefore, these results showed $Ca^{2+}$ to be effective factor in the cell wall formation. Isozyme pattern of peroxidase was investigated in the callus and hairy root induced from potato tuber.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.111.2-111.2
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• 2011

최근 지구온난화로 인해 국제적으로 이산화탄소 저감에 대한 연구가 진행되고 있으며 특히, 이산화탄소의 분리 및 유용물질 전환 등의 다양한 방법에 대한 연구가 활발히 이루어지고 있다. 이산화탄소를 메탄으로 전환시키는 생물학적 반응은 acetotrophic methanogen, hydrogenotrophic methanogen 등의 미생물이 관여한다. 본 연구에서는 hydrogenotrohpic methanogen을 이용하여 메탄으로 전환하고자 하였다. 이를 위해 이산화탄소와 수소의 체류시간에 대한 연구를 진행하였으며, 선행 연구로 혐기성슬러지의 혼합배양균으로부터 hydrogenotrophic methanogen을 우점종화 하기 위해 고정층 반응기를 이용하여 이산화탄소와 수소 가스를 주입하여 고농도로 배양하였다. 그 결과, 반응기내의 이산화탄소의 메탄전환 균주로써 수소를 환원제로 이용하는 hydrogenotrophic methanogen이 배양되었음을 확인하였다. 이산화탄소와 수소가스의 체류시간에 따른 이산화탄소의 생물학적 메탄 전환 실험 결과, 약 4시간에서 이산화탄소의 저감률이 99%이었으며, 체류시간이 2시간, 1.5시간인 경우 이산화탄소의 저감률은 각각 71%, 68% 이었다.

• Journal of Life Science
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• v.13 no.4
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• pp.511-515
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• 2003

White rot fungi, Phanerochaete sp. EJ-31L, was evaluated for its ability to decolorize Remazol Black B, an azo dye that is a widespread pollutant in the wastewater of textile industry. It was observed that extent of decolorization by Phanerochaete sp. EJ-31L was dependent on the concentrations of co-carbon and nitrogen source. Effects of agitation and aeration were studied, and agitated culture at aeration condition resulted in greater extent of decolorization than static culture. Remazol Black B was readily decolorized up to 95% within 64 hr by Phanerochaete sp. EJ-31L.

• Bulletin of Food Technology
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• v.15 no.1
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• pp.3-15
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• 2002

이 논문은 이소플라본과 골조직의 관계에 대하여 발표된 역학조사, 사람과 동물을 대상으로한 실험, 조직세포연구, 세포배양에서의 연구들을 포괄하고 있다. 가장 중요한 결론은 적절한 양을 투여했을 때 이소플라본, 특히 제니스테인과 다이드제인은 골밀도 증진에 효과가 있다는 것이다. 쥐를 대상으로 한 실험에서 적정 골밀도 향상 효과를 나타내기 위한 이소플라본의 invivo 투여량은 에스트라디올보다 보통 1000배가 높았다. 또한 적정 투여량보다 낮거나 높은 농도에서는 이소플라본은 뼈에 유익한 효과가 없었으며 그 때문에 이중적인 효과를 발휘한다. 유사 조골세포를 배양한 결과 중 in vitro실험에서 제니스테인의 효과는 관찰되었으나 다이드제인의 효과는 나타나지 않았다. 일반적으로 대두와 대두제품에 존재하는 이소플라본은 동물이나 사람이 적정한 양으로 섭취하였을 때 약한 에스트로겐 효과를 발휘하지만 고농도에서는 오히려 해로운 효과가 관찰될 수도 있다. 제니스테인에 대한 세포수준의 연구에서는 고농도 투여시에는 정상적인 세포기능의 소실을 유도하기도 한다고 제안되었으며, 반면 다이드제인은 같은 농도에서 유사 조골세포에 유익한 효과를 발휘한다고 하였다. 사람이 식이로부터 섭취하는 양은 부작용을 초래할 정도가 되기는 힘들다. 따라서 이소플라본은 적정한 양으로 섭취했을 때 사람의 골질량을 유지하거나 골질량을 증가시키는 잠재적인 능력이 있음이 분명하다.

• KSBB Journal
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• v.7 no.4
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• pp.271-277
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• 1992

Rat hepatocytes were isolated by collagenase perfusion method and cultured on the collagen coated dish or on the floating collagen membrane. Using the primary cu1tured hepatocytes, the efficiency of cell attachment and the hepatic functions such as gluconeogenesis, ureogenesis and albumin synthesis were studied. The cell viability was kept above 50% until 5 days and the hepatic functions of ammonia metabolism and albumin synthesis were maintained until 7 days. Floating collagen membrane was found to be more efficient than the collagen coated dish for the maintenance of hepatic function in-vitro.

• KSBB Journal
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• v.11 no.3
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• pp.311-316
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• 1996

The effects of polyethylene glycol (PEG) on glucoamylase production in recombinant Saccharomyces cerevisiae were studied. By PEG addition, the cell growth was not affected. However, the glucoamylase production was increased in all range of PEG molecular weights and concentrations. The optimal molecular weight of PEG was 6000 and the optimal concentration was 1g/L. Using these optimals, the extracellular glucoamylase activity was 23% higher in PEG-containing medium than that in medium without PEG.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.356-360
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• 2003

Hundreds of microbial species were isolated and collected from dye-processing wastewater. Among them three species (named as RA1, RA2, and YA1) showed excellent ability of dye degradation. Especially, YA1 species could remove 53% of dye in 24 hours. To find optimal growth conditions for the isolated species, further research is going on.