• Title/Summary/Keyword: 약제 저항성

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Resistance Development and Cross-Resistance of Diamondback Moth (Lepidoptera: Plutellidae) by Single Selection of Several Insecticides (단제도태에 의한 배추좀나방(Plutella xylostella)의 약제저항성 발당과 교차저항성에 관한 연구)

  • 조영식;이승찬
    • Korean journal of applied entomology
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    • v.33 no.4
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    • pp.242-249
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    • 1994
  • These studles were conducted to investigate the development of chem~cal resistance and cross.resista. nce m dlarnondback moth (Piuteiia xylostella L). The resistance development of diamondback moth greatly vaned under single selection of five insectlades. The tnflumuron and lambda cyhalothnn strains at 8th selected generation showed 374- and 29.l.fold resistant levels. respectively, as compared with the susceptible strain. However, the Baciilus thuringiensis-seleded at 8th selected generatLon exhibited 240-fold resistant level. and the prothiophos-selected at 8th generation revealed 14.3-fold resistant !eve1 while the cartap hydrochloride-selected at 8th generailon showed 9.1-fold resistant level Prothiophos- selected strain showed low cross-resistance level to cartap hydrochloride, while this strain exhibited no cross-resistance of 1.3 to 2.8-fold to other msectlcides. Cartap hydrochlonde- seleded strain showed 19.9-fold. a high cross-resistance to lambda cyhalothrin, but this strain showed 2.2-34 fold, no cross resistance to other insecticide. Lambda cyhalothnn-selected strain exhibited cross-resistance to cartap hydrochlolide and prothiophas. Triflumuron-seleded strain showed 1.3-4.9 fold. no cross-resistance to other ~nsectic~dTe he B. ihuring~ensis-selededs train showed no cross-resistance to other insecticides.

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Insecticide Resistance Mechanism in the Spiraea Aphid, Aphis citricola (van der Goot) (조팝나무진딧물의 살충제 저항성 메카니즘에 관한 연구)

  • ;;本山直樹
    • Korean journal of applied entomology
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    • v.34 no.2
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    • pp.89-94
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    • 1995
  • Resistance mechanism of organophosphorus insecticides (OPs) in the spiraea aphid, whose populations were originated from several apple orchards where various OPs were frequently sprayed, was investigated. For Wonju population to which insecticides were not sprayed, resistance ratios (BRs) for pirimicarb, phosphamidon, and demeton S-methyl re 49, 31, and 5, respectively. However, for Yesan population to which OPs were sprayed 5 times, RRs for pirimicarb, phosphamidn, and dementon were 830, 536, and 204, respectively. The esterase activities of Wonju and Yesean populations increased by 44.5 and 92.0% compare to that of Kwagju population. R/S a values (S clone Kwangju population; R clone: Iechon opulation) for acetylcholinesterase (AChE) inhibition (${I}_{50}$) by pirimicarb and phosphamidon were 299.2 and 186.0, respectively. Our results indicate that increase of esterase activity and reduction of AChE sensitivity seemed to contribute insecticide resistance of the spiraea aphid.

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Isolation of Plasmid Korean Copper-Resistant Xanthomonas campestris pv. vesicatoria (한국에서 분리한 고추 더뎅이병균의 구리저항성 Plasmid)

  • 박의훈;조용섭
    • Korean Journal Plant Pathology
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    • v.12 no.2
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    • pp.156-161
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    • 1996
  • 세계적으로 구리약제에 대해서 저항성을 나타내는 균주들이 발견되있으며, 이들은 구리약제의 방제효과를 감소시켰다. 국내에서도, 구리 저항성 균주 Xanthomonas campestris. pv. vesicatoria HN94-2, -3, -4, -5, -6 들이 천안지역의 고추재배지에서 처음으로 분리되었으며, 이들 균주들의 nutrient agar에서 황산구리(CuSo\ulcorner)에 대한 최소억제농도(minimum inhibitory concentration, MIC)는 1.4~1.6 mM이었다. 이들은 모두 황산아연(ZnSO\ulcorner)에 대해서는 감수성을 보여, 구리저항성 균주에 대한 방제약제로서 아연 함유 약제를 사용할 수 있을 것이다. 분리된 균주중 HN94-2와 HN94-6을 이용하여 접합(conjugation)을 통해 구리저항성의 전파를 실험한 결과, 이들 두 균주 모두 구리감수성 균주에게 4.3$\times$10\ulcorner에서 1.0$\times$10\ulcorner(transconjugant/donor)의 정도로 구리 저항성이 전이되었다. 이들 HN94-2와 HN94-6 균주의 구리정항성 유전자들은 약 200 kb 정도의 커다란 플라스미드(plasmid)에 존재하며, 이들은 각각 pXVK9402와 pXVK9406이라 명명되었다.

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Insecticide Resistance Monitoring of Bemisia tabaci (Hemiptera: Aleyrodidae) in Korea (전국 담배가루이 약제 저항성 조사)

  • Kim, Sanghyeon;Kim, Sung Jin;Cho, Susie;Lee, Si Hyeock
    • Korean journal of applied entomology
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    • v.60 no.2
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    • pp.167-173
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    • 2021
  • Sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is an insect pest with sucking mouth part and globally attacks diverse crops and vegetables. Since first reported in 1998, it is now widely spread in many regions in Korea. Due to insecticide resistance, it is necessary for optimal control of the whiteflies to select effective insecticides with precise insecticide resistance monitoring. In this study, B. tabaci individuals were collected from 12 regions in 7 provinces of Korea from June to September in 2020. Using these field populations, insecticide resistance levels were monitored using a residual contact bioassay along with molecular markers. Bioassay results revealed that B. tabaci possessed high levels of resistance to five insecticides exhibiting different modes of action: dinotefuran, spinosad, emamectin benzoate, chlorfenapyr, and bifenthrin. In addition, quantitative sequencing in target sites of organophosphate and pyrethroid insecticides revealed that point mutations reached to saturated or near-saturated levels across the country. This suggests that insecticide resistance management is required for effective control of B. tabaci populations in Korea.

Resistance of Diamondback Moth(Plutella xylostella L.: Yponomeutidae: Lepidoptera) against Bacillus thuringiensis Berliner (BT제에 대한 배추좀나방의 약제저항성)

  • 송승석
    • Korean journal of applied entomology
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    • v.30 no.4
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    • pp.291-293
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    • 1991
  • Inter-regional difference in median lethal concentration of Bacillus thuringiensis diamondback moth was disclosed to be high. Pyungchang strain showed at most 41 times resistance compared to KN-IA strain of which $LC_{50}$ value was 5.5 ppm. and Oksan strain Showed 11 times resistance. The JMC strain, susceptible to pyrethroids, also showed 12 times resistance against B.T., suggesting difference in resistance mechanism between pyrethroid and B.t.

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Chemical Resistance and Control of Dollar Spot Caused by Sclerotinia homoeocarpa on Turfgrass of Golf Courses in Korea (한국 골프장 잔디에서의 Sclerotinia homoeocarpa의 약제 저항성 및 방제)

  • Kim, Jeong-Ho;Choi, Hee-Youl;Shim, Gyu-Yul;Kim, Young-Ho
    • Asian Journal of Turfgrass Science
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    • v.24 no.2
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    • pp.170-175
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    • 2010
  • A total of 24 isolates of S. homoeocarpa were isolated from 16 golf courses in Korea. Chemical resistance of 24 isolates was determined by in vitro fungal growth on fungicide-amended media with thiophanatemethyl (Benzimidazole fungicide family), tebuconazole (demethylation inhibitor fungicide family: DMI), and iprodione (dicarboximide fungicide family). Results indicated that 83.3% of 24 isolates were resistant to iprodione, 62.5% resistant to thiophanate-methyl and 0% resistant to tebuconazale. The dual resistance of iprodione and thiophanate-methyl was 58.3.%. Occurrence rate of fungicide resistance of thiophanate-methyl and iprodione had no relation to turfgrass varieties and isolated locations of pathogen. In the filed test, procymidone, boscalid, and fluquinconazole+pyrimethanil effectively controlled the dollar spot of creeping bentgrass.

Occurrence of Botrytis cinerea Resistant to Dicarboximide Fungicides on Strawberries in Greenhouses (시설재배(施設栽培) 딸기 포장(圃場)에서 Dicarboximide 계(系) 약제저항성(藥劑抵抗性) 잿빛곰팡이병균(病菌)의 발생(發生))

  • Yu, Seung Hun;Park, Jeong Hun
    • Korean Journal of Agricultural Science
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    • v.19 no.2
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    • pp.170-178
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    • 1992
  • Isolates of Botrytis cinerea resistant to dicarboximide fungicides were collected from strawberry fields in greenhouses in spring and early summer of 1990. Five out of 9 isolates of B. cinera were resistant, which showed mycerial growth on PDA containing dicarboximide fungicides(procymidone and vinclozolin) with concentrations of 100, 400 and $1,600{\mu}g/ml$. The minimal inhibitory concentration(MIC) values of the dicaboximide-resistant isolates was more than $6,400{\mu}g/ml$, while that of the sensitive isolates was less than $6.25{\mu}g/ml$. The germination ratio of conidia of the resistant isolates on PDA containing procymidone and vincolozolin was more than 95%, while that of the sensitive was less than 15%. The procymidone-resistant isolates were also resistant to vinclozolin, showing cross-resistant between the fungicides, but cross-resistant was not observed between the dicarboximides and dichlofluanid. Resistance to benomyl was also found in all the dicarboximide resistant isolates. Occurrence frequency of dicarboximide-resistant isolates out of 223 isolates was about 40%. The resistant isolates were widely distributed throughout Korea.

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Investigation of resistance mechanism for Botrytis cinerea to procymidone (잿빛곰팡이병균(Botrytis cinerea)의 Procymidone 약제 저항성 요인 구명)

  • Cho, Jeong-Rye;Lee, Kyu-Seung
    • Korean Journal of Environmental Agriculture
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    • v.14 no.3
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    • pp.319-328
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    • 1995
  • This study was carried out to investigate the resistance mechanism by three different kinds of procymidone-resistant and susceptible isolates of Botrytis cinerea which had been collected from green houses. The average resistance level of the resistant strains was 1,000 times higher than that of susceptible ones. Also, it was revealed that the resistance was not originated from components excreted by Botrytis cinerea, based on the result obtained from the treatment with piperonyl butoxide and triphenyl phosphate as an inhibitor of monooxygenase and esterase, respectively. The total lipod content of resistant strains was 1.3 times higher than that of susceptible ones, among fatty acids, palmitic acid, stearic acid, and linoleic and being 3.0, 2.5, and 2.0 times higher, respectinely. Also slight differences in sterol contents and components were observed. The crude chitin content was slightly higher in susceptible strains but contents of N-acetyl glucosamine, a hydrolysate of chitin, were about 2 times higher in resistant ones.

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