• Title/Summary/Keyword: 약제 선발

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Chemical screening for control of Metalaxyl-resistant tobacco black shank disease (Metalaxyl 저항성 담배역병균(Phytophthora nicotianae) 방제를 위한 약제 선발)

  • Kang, Yue-Gyu;Chung, Yun-Hwa
    • Research in Plant Disease
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    • v.9 no.4
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    • pp.224-228
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    • 2003
  • Five commercial chemicals, Dimethomorph, Foseyl-Al, Oxadixyl + Mancozeb, Propamocarb hydrochloride, and Metalaxyl, were tested for control of tobacco black shank (Phytophthora nicotianae) and/or delaying buildup of the resistant population to Metalaxyl which has been used for control of the disease in Korean tobacco farms. Propamocarb hydrochloride seemed to have a cross-resistance to the Metalaxyl resistant isolates showing similar response with Metalaxyl in vitro. Meanwhile, Dimethomorph+Mancozeb and Oxadixyl+Mancozeb were selected to be the promising chemicals which are able to be alternative to Metalaxyl for the black shank control in accordance with in vitro and in field trial.

Role of general esterases in deltamethrin resistance mechanism of diamondback moth, Plutrlla xylostella L. (배추좀나방(Pulltella xylostella L.)의 deltamethrin 저항성 기작에 관한 에스테라제의 역할)

  • 김용균;장동걸
    • Korean journal of applied entomology
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    • v.35 no.1
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    • pp.74-79
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    • 1996
  • General esterases were analysed quantitatively and qualitatively to see their role in deltamethrin resistance mechanisms of the diamondback moth, Plutella xylostella L. Selection with 0.1 g of deltamethrin in each generation induced the moth to decrease susceptibility to the insecticide and to increase esterase activities of the fourth instar larvae. Both characters were highly correlated so that the correlation coefficient (r) between LDSo @g) of deltamethrin and esterase activities (~M/min/pg) was 0.9918 (P=0.0082). Nondenaturing PAGE (6%) separated 17 esterase bands from the whole body extracts of the fourth instar larvae. Deltamethrin-selected populations had fewer esterase bands than had the unselected. Four esterase bands (E3, E4, Ell, and E13) were, however, specific to deltamethrin-selected populations.

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Studies on Selection of Less Toxic Insecticides for the Aerial Control of Pine Needle Gall Midge (Thecodiplosis) japonensis Uchida et Inouye) (솔잎혹파리 항공방제용 저독성 농약선발연구)

  • 이형래;변병호
    • Korean journal of applied entomology
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    • v.33 no.3
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    • pp.201-206
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    • 1994
  • Trunk implantation method of phosphamidon 50% Lq.is commonly applied to control the pine needle gall midge (Thecodiplosis laponensts Uchida et Inouye) Since trunk implantaion is normally practiced during the late Spring, it is often difficult to accommodate necessay labor in mral area. As an alternative, aerial spraylng of less toxic ~nsecticide was designed. Usage of less toxic insecticide in the aenal control of pine needle gall midge can reduce the damage to forest ecosystem. The buproferin, one of the insect growth regulator, was selected at d~fferent rate of dilutions and the treatments effects ruere evaluated at different date and time When 50 t~mes diluted solution of buprofezin 40% SC was treated on different period, there was no significant difference in dficacies. the control efficacies of buproferin 40% SC was measured by occurrences (%) of gall formation of the pine needle gall midge using a ultra low volume (ULV) sprayer and the dilutions of 10X 30X and 50X of buprofez~n 40% SC gave efficacies. 72.4. 57.6 and 8.4, respectively.

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Selction of Useful Chemicals Reducing Soybean -Sprout Rot (콩나물 부패경감에 유용한 약제 선발)

  • 박의호;최연식
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.4
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    • pp.487-493
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    • 1995
  • This study was carried out to select useful chemicals to control pathogens inciting soybean-sprout rot. Pathogen strains were isolated from decaying soybean seedlings. The isolated strains were identified as Pseudomonas spp. (Pse. strains) through microbiological test, however, no rot-inciting fungus was isolated. Eight food additives were tested with different concentrations in controlling pathogens and harmfulness. Five chemicals(over 5% cone.) including sodium hypochlorite apparently inhibited the growth of Pse. strains on media plate, however, sodium hypochlorite was discarded due to its severe germination inhibition. Propionic acid and acetic acid inhibited the growth of Pse. strains more effectively than calcium propionate and sodium propionate relatively. As no harmful effect on seed germination and no growth retard of soybean sprouts were observed by those chemicals with lower concentration(0.5∼1.0%), these chemicals were considered to be applicable to sprout rot control judged by the effectiveness and permissible concentration as food additives.

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Selection of Low Toxic Pesticides to Microplites mediator (큰밤고치벌(Microplites mediator)에 대한 저독성 약제 선발)

  • Choi, Byeong-Ryeol;Kwon, Min;Lee, Si-Woo;Park, Hyung-Man
    • The Korean Journal of Pesticide Science
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    • v.12 no.2
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    • pp.177-183
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    • 2008
  • For the development of integrated pest management system by harmonizing biological and chemical control, some experiments were carried out to select low toxic pesticides against natural enemies. Leaf dipping method, body dipping method, and diet treatment method were used for the toxicity evaluation against Microplites mediator adults. We tested 49 different pesticides (34 insecticides, 11 fungicides, 4 herbicides) at recommending concentration commonly used to control insect pests, disease and up-land weeds in chinese cabbage field. 16 insecticides, 11 fungicides, and 4 herbicides were shown to be low toxic to Microplites mediator adults in the treatment of body dipping.

Studies on the Chemical Resistance of Phytopathogenic Bacteria II. Selective Effect of Chemical Resistance on the Rice Bacterial Leaf Blight Pathogen, Xanthomonas oryzae (Uyeda et Ishiyama) Dowson, to Agrepto (식물병원성 세균의 약제저항성에 관한 연구 II. 벼흰빛잎마름병균의 Agrepto에 대한 저항성의 선발효과)

  • Cho W. C.;Shim J. W.
    • Korean journal of applied entomology
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    • v.16 no.4 s.33
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    • pp.229-234
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    • 1977
  • The experiments were carried out to investigate the selection effects on the drug resistance to Agrepto in Xanthomonas oryzae, the causal bacteria of rice bacterial leaf blight. The results obtained were as follows. 1, The Agrepto was stable at the heat treatment of $105^{\circ}C$ for 20 minutes, when the drug was added in the media. 2. The local isolates of the bacteria, 75-6 and 75-9, showed the different resistante reaction, when they were selected by the Agrepto contained media which concentrations of 10r9/m1 and 100ug/ml. 3. The individuals shelving high degree of resistance, which can grow on the media contained 10,000ug/m1 of Agrepto, could selected by the concentrations as low as 10ug/ml and 100ug/m1, in one generation. 4. The highly resistant isolates which selected by 3-stepwise selections such as 100ug/ml, 3,000ug/ml and 10,000/ml plots, showed nearly normal growth at the media contained 100ug/ml of Agrepto 5. When the isolate 75-9 was selected at the 100ug/m1 of concentration, showed various degrees of resistance, indicating that the isolate may be composed of resistance groups that lower than 500ug/ml, between 500-1,000ug/ml and 1,000-3,000ug/ml, to the Agrept.

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Variation in Insecticide Susceptibilities of the Beet Armyworm, Spodoptera exigua (Hubner): Esterase and Acetylcholinesterase Activities (파밤나방(Spodoptera exigua (Hubner))의 살충제 감수성 변이-에스테라제와 아세틸콜린에스테라제 활력)

  • 김용균;이준익;강성영;한상찬
    • Korean journal of applied entomology
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    • v.36 no.2
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    • pp.172-178
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    • 1997
  • There was a great variation in insecticide susceptibilities among field and laboratory populations of the beet armyworm, Spodoptera exigua (Hiibner). Unselected laboratory population, which had been reared for 6-7 generations in our laboratory without exposure to insecticides, was more susceptible than its parental field population in all tested insecticides. Two selected laboratory populations with parathion or deltamethrin showed much higher insecticide tolerance than did the unselected laboratory population in their own selection insecticide. The variation of the insecticide susceptibilities was highly correlated with esterase and acetylcholinesterase activities. Field and the selected laboratory populations had lower acetylcholinesterase activities and higher esterase activities than did the unselected laboratory population. Acetylcholinesterase of the field and the selected laboratory populations had higher Km values than did that of the unselected. In a population, Km values were varied among different developmental stages; acetylcholinesterase of the fifth instar larvae had the highest Km value among those of the other larval stages. Twenty one esterase bands were separated on 6.5% nondenaturing polyacrylamide gel from the whole body extracts of the fifth instar larvae. E2, E7, E8, Ell, El6, and El7 esterase bands were developed more frequently in the insecticides-selected populations than in the unselected population. These results suggest that the variation of insecticide susceptibilities of the beet armyworm includes both biochemical mechanisms: target site insensitivity and enhanced activity of detoxification enzyme.

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Selection of Low Toxic Pesticides and Residual Toxicity to Cotesia glomerata (배추나비고치벌 (Cotesia glomerata)에 대한 저독성 약제 및 잔류독성)

  • Choi, Byeong-Ryeol;Lee, Si-Woo;Park, Hyung-Man
    • Korean journal of applied entomology
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    • v.46 no.2
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    • pp.251-259
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    • 2007
  • For the development of integrated pest management system by harmonizing biological and chemical control, some experiments wee carried out to select low toxic pesticides against natural enemies. and their residual toxicity were evaluated. Leaf dipping method, body dipping method, and diet treatment method were set up for the toxicity evaluation against Cotesia glomerata adults. We had tested 46 different pesticides (31 insecticides, 11 fungicides, 4 herbicides) at recommending concentration commonly used to control diamond back moth, disease and up-land weeds in chinese cabbage field. Twenty three insecticides, eleven fungicides, and four herbicides were shown to be low toxic to C. glomerata adults in the treatment of body dipping. After insecticide spraying at recommending dose on the chinese cabbage, we examined residual effect of insecticides by introducing natural enemies on different days. Safety interval for the introduction of C. glomerata adults was established according to the residual toxicity of pesticides. Safe insecticides for the introduction of C. glomerata adults at one day after treatment (DAT) were thiacloprid, acephate, chlorfenapyr, clothianidin and at 3 DAT were imidacloprid, deltamethrin, thiamethoxam, dimethylvinphos, emamectin benzoate.