• Journal of Mushroom
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• v.12 no.1
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• pp.29-34
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• 2014

Mycelial viability and cultivation characteristics of strains of Hypsizygus marmoreus after long-term storage were investigated. The experimental conditions were the storage at $4^{\circ}C$ using a slant culture technique with or without mineral oil, and in liquid nitrogen tank in the presence of 10% glycerol or 10% glycerol with 5% trehalose. The myceila of four strains of H. marmoreus were thawed at 9, 21, 33, and 45 months after beginning of the storage, and then the growth of the mycelia was measured on a PDA plate with serial transfers to new plate for the recovery. The mycelial growth data after 45 months showed that the mycelia were mostly viable but not fully active particularly when they were stored in liquid nitrogen with 10% glycerol. The growth activity could be fully recovered after second transfer to new PDA plate. Cultivation of mushroom fruiting body using the recovered mycelia also demonstrated that the storage methods employed in this work were applicable for the long-term storage of H. marmoreus.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 1997.04a
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• pp.122-128
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• 1997

초전도체의 임계온도를 높이는 연구들이 수행되어 임계온도가 액체질소의 비등점(77K)보다 높은 산화물 고온 초전도체가 1986년에 발견되었고, 최근에는 $10^4A/cm^2$이상의 임계전류밀도를 갖는 덩어리형 고온 초전도체가 용융공정을 통해 개발되었으며, 산화물 고온 초전도체 $YBa_2 Cu_3 O_{7-y}$은 산소 조성이 7에 가까우며 임계온도가 95K로서 현재 가장 많이 사용되고 있다. 응용 분야로서는 수송분야, 의료분야, 전력분야, 그리고 기초과학분야등이 있고, 향후 상온 초전도체의 출현으로 인한 응용범위의 확대는 획기적일 것으로 예측되어 진다. 이중에 10$^{-8}$이하의 마찰계수를 갖는 초전도 마그네틱 베어링을 플라이휠 같은 에너지 저장장치에 적용시키는 연구가 국내외적으로 진행되고 있다. 여러 선진국에서는 용융공정된 초전도체를 이용하여 실험단계적인 플라이휠의 설계, 제작 및 성능실험을 진행중이거나 완성하였고, 제2단계 목표로서 실용화를 위한 대규모 플라이휠의 설계와 제작에 대한 연구가 진행중이다.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 1997.10a
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• pp.167-172
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• 1997

초전도체를 기계요소중 하나인 베어링에 응용하기 위한 연구는 임계온도가 액체질소의 비등점(77K)보다 높은 산화물 고온 초전도체가 발견된 이후 시작되었으며, 특히 최근에는 $10^4A/cm^2$이상의 임계전류밀도를 갖는 덩어리형 고온 초전도체가 용융공정을 통해 개발되어, 큰 부하지지력을 갖고 10$^{-8}$ 이하의 마찰계수를 갖는 초전도 마그네틱 베어링으로서 플라이휠 같은 에너지 저장장치에 적용시키는 연구가 국내외적으로 진행되고 있다. 고온 초전도체를 사용한 반발식 수동형 미그네틱 베어링은 Meissner effect뿐 아니라 Fluxpinning effect에 의해 자체적으로 외란에 대한 위치안정성을 가지며, 히스테리시스 손실에 의한 에너지 소산을 통해 외란에 대해 강한 감쇠능력을 가진다는 장점을 가지고 있으며, 대중량을 지지할 수 있다. 이러한 초전도체의 특성에 관한 정량적 수치해석은 초전도 베어링의 설계에 필수적이나 아직 국내에서는 그러한 시도가 없었다. 이러한 여건을 고려하여 본 연구에서는 초전도체와 자석간의 부상력 변화를 2차원 Slab모델로 수치해석하여 히스테리시스라는 주요한 특성을 고찰하고자 한다.

• Journal of Energy Engineering
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• v.5 no.1
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• pp.19-27
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• 1996

Self-pressurization of cylindrical container of cryogen is numerically analyzed. The container is axi-symmetric and heated from side wall with constant heat flux. Natural convection by external heat flux is studied numerically using finite difference method. Oxygen, nytrogen and hydrogen are working fluids in this paper. Liquid is considered incompressible fluid and vapor is assumed to behave as gas meeting with virial equation of gas. The Second virial coefficients of gas are obtained from Lennard-jones model. The important variables which have effects on self-pressurization are external heat flux, heat capacity of wall and initial ullage in container. The most important variable of them is external heat flux. The pressure rise calculated from the virial gas model is slightly different from that calculated using Ideal gas model for oxygen.

• Food Science and Preservation
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• v.11 no.1
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• pp.111-116
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• 2004

The optimum cultural conditions for production of red pigment from Monascus rubber KCTC 6122 is liquid culture were studied. Monascus robber KCTC 6122 was shown to give the maximum production of red pigment in the medium containing 4％ rice powder, 0.2％ NaNO$_3$, 0.3％ Na$_2$HP0$_4$ and 0.15％ MgSO$_4$. The optimum culture conditions, temperature, initial pH and shaking speed were 30$^{\circ}C$, 6.5 and 150 rpm, respectively. The red pigment production reached a maximum level at 8days of cultivation.

• Korean Journal of Medicinal Crop Science
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• v.7 no.1
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• pp.1-6
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• 1999

Basic informations for direct somatic embryo formation in Rehmannja glutinosa Lib. were obtained in 500ml erlenmyer flask. The ratio of ammonium to nitrate nitrogen of 825(mg/l) : 1900(mg/l) was proper condition for somatic embryo formation from stem and petiole explants and 3% sucrose was the most effective carbon source. Full strength MS medium with 2mg/l BA was better than LS medium for somatic embryogenesis. The initial pH 5.7 of medium(full strength MS with 2.0mg/l BA and 0.1mg/l NAA) was good for embryo production. Potassium ion was taken up rapidly within 2 weeks. while $Ca^{++}$ and $Mg^{++}$ ion contents were almost constant during culture period. Sucrose hydrolysis occurred throughout the culture, while glucose and fructose were absorbed simultaneously from the third week of culture.

• Proceedings of the SAREK Conference
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• 2008.06a
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• pp.142-147
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• 2008

This paper reports an analysis of self-pressurization in a closed cryogenic liquid storage system and its comparison with experimental data using liquid nitrogen. Partial equilibrium model(PEM), revised thermodynamic analysis of homogeneous model, has been applied for the pressurization in a closed tank. The vapor and liquid bulk temperature and the liquid-vapor interface temperature are separately calculated as their own representative values in this analysis. The analysis results of the partial equilibrium model are compared with the experimental data and other preceding homogeneous temperature models for validation.

• Korean Journal of Microbiology
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• v.36 no.1
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• pp.74-78
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• 2000

Vibrio, Photobacterium, Alteromonas and Xenorhabdus species are capable of emitting light, called bioluminescence. They exist in marine, freshwater and terrestrial environments. Bacterial bioluminescent reaction is that reduced riboflavin phosphates and a long-chain aldehyde are oxidized in the presence of molecular oxygen and enzyme luciferase. This experiment aims to develop the proper culture media and to optimize the storage condition for the recovery of bioluminescent activity in Photobacterium phosphoreum. The Luria broth (LB) medium was modified for cultivation of Photobacterium phophoreum, called as modified LB(mLB) medium. The mLB medium is LB fortified with 3% glycerol and 1.5% NaCl. In mLB medium. bacterial growth and bioluminescent activity are 25% higher than those in a Nutrient broth medium. When the cell stocks were stored at $-20^{\circ}C$, $-70^{\circ}C$ and LN2 for 3 months, cell growth and bioluminescent activity of culture after stored at $-20^{\circ}C$ were better than those of other treatments. The highest bioluminescent activity obtained at the late exponential phase in all treatments. When the cell stock was freeze-dried with 5% adonitol as a cryoprotectant, the recovery of cell was better than those of control and freeze-dried cell stock without addition of cryoprotectant.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2007.04a
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• pp.58-64
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• 2007

Propellant pressurization system in liquid rocket propulsion system plays a role supplying pressurant gas at a controlled pressure into the ullage space of propellant tanks. The most important design parameter for such propellant pressurization system is the temperature of pressurant gas fed from pressurant tank, which is placed inside of cryogenic propellant tank. Such pressurant is gaseous state, of which density is very sensitive to the temperature of pressurant. Previous investigation dealt with thermal correlation of pressurant and external fluid at room temperature. This study investigates the temperature variation of cryogenic pressurant (GHe) at the time when the pressurant is coming out of pressurant tank, which is submerged in a liquid oxygen, experimentally as well as numerically.

• Journal of Advanced Marine Engineering and Technology
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• v.39 no.9
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• pp.876-880
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• 2015

In 2016, the IMO's new rules for an 80% reduction in NOx emissions in newly built ships will necessitate the use of LNG as a clean fuel. So far, the developed European countries have led the development of LNG bunkering ships and related facilities. An LNG bunkering system stores LNG in a horizontal or vertical IMO "C"-Type tank insulated with perlite powder, and a vacuum in the annular space between the double walls, like the cryogenic liquid nitrogen tank. Current storage tanks have high heat leakage, evaporating over 2.0% daily, and are difficult to build with the required vacuum. A more efficiently insulated storage tank could reduce the evaporation rate. This research carried out thermal analysis on a new effective insulation method that separates high vacuum in the annular space between two tanks with a solid insulation material, such as urethane foam, lining the outer vessel. This highly efficient insulation system obtained an evaporation rate of 0.03% per day under a $10^{-3}torr$ vacuum, and an evaporation rate of 0.11% at $10^{-45}torr$. Even if the space loses its vacuum, the new insulation system showed a lower evaporation rate of 4.12% than the present perlite system of 4.9%. This newly developed tank can increase the efficiency of LNG storage tank and may help keep LNG bunkering systems safe.