• Title/Summary/Keyword: 암조건

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Simultaneous Determination of Urinary Phytoestrogens and Estrogens by Gas Chromatography/Mass spectrometry (GC/MS에 의한 뇨 중 Phytoestrogen과 Estrogen의 동시 분석)

  • Yang, Yoon Jung;Lee, Seon Hwa;Chung, Bong Chul
    • Analytical Science and Technology
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    • 제11권5호
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    • pp.374-385
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    • 1998
  • Phytoestrogens are biologically active compounds derived from plants foods. It had been suggested that phytoestrogens, by inhibiting aromatase in peripheral and/or cancer cells and lowering estrogen levels, may play a protective role as antipromotional compounds during growth of estrogen-dependent cancers. Therefore, simultaneous analysis of estrogens and phytoestrogens is necessary to elucidate the possible involvement of phytoestrogens in estrogen metabolism. In this view, we developed a simple and reproducible procedure to quantitatively determine estrogen and phytoestrogen metabolites. The proposed method consisted of solid phase extraction using preconditioned Serdolit AD-2 resin, enzyme hydrolysis with ${\beta}$-glucuronidase/arylsulfatase from Helix pomatia, liquid-liquid extraction and TMS-ether derivatization. And the final determination was carried out by gas chromatography/mass spectrometry (GC/MS) in selected ion monitoring mode (SIM). The precision and accuracy of this method was evaluated through within-a-day and day-to-day test. Recovery range and detection limit were 71.96~105.66%, 2~4 ng/mL, respectively. Using this method, 17 estrogen and 5 phytoestrogen compositions in urine of normal subjects were analyzed. It was found that amounts and relative distribution of urinary phytoestrigens and estrogens showed different pattern in male and female subjects.

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Case Study on the Causes for the Failure of Large Scale Rock Mass Slope Composed of Metasedimentary Rocks (변성퇴적암류로 구성된 대규모 암반사면의 붕괴원인 분석에 관한 사례 연구)

  • Park, Boo-Seong;Jo, Hyun;Cha, Seung-Hun;Lee, Ki-Hwan
    • Tunnel and Underground Space
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    • 제16권6호
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    • pp.506-525
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    • 2006
  • For the design of large scale rock slope which has complex formations and geological structures, generally, insufficiency of geotechnical investigations and laboratory tests are the main factors of slope failures doling construction. In such case, remedial measures to stabilize slope should be selected and applied through reliable investigations and analysis considering the geotechnical characteristics. The rock slope of this study, one of the largest cut slopes in Korea with a length of 520.0 m and maximum height of 122.0 m consists of metasedimentary rocks. And a case study on the causes of large-scale rock slope failure was carried out by analysis of landslides history and site investigations during construction. When the slope with the original design slope of 0.7: 1.0 (H:V) was partially constructed, the slope failure was occurred due to the factors such as poor conditions of rocks (weathered zone, coaly shale and fault shear zone), various discontinuities (joints, foliations and faults), severe rain storm and so on. The types of failures were rockfall, circular failure, wedge failure and the combination of these types. So, the design of slope was changed three times to ensure long-term slope stability. This paper is intended to be a useful reference for analyzing and estimating the stability of rock slopes whose site conditions are similar to those of this study site such as geological structures and geotechnical properties.

Effects of Temperature on Development of Oligota kashmirica benefica(Coleoptera: Sthphylinidae) and Its Seasonal Fluctuation in Yuzu Orchards (O1igota kashmirica benefica (딱정벌레목: 반날개과) 발육에 미치는 온도의 영향과 유자과원에서의 발생소장)

  • 최덕수;김규진;박종대
    • Korean journal of applied entomology
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    • 제41권3호
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    • pp.199-204
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    • 2002
  • Population fluctuation of O. kashmirica benefica, a major natural enemy of citrus red mite (Panonychus citri), was surveyed from 1997 to 2000 in yuzu orchards in Goheung region and, developmental periods, developmental threshold temperature (DT) and effective cumulative temperature (ET) were estimated. Under four constant temperatures of 15, 20, 25 and 30$\^{C}$, developmental periods from egg to adult of the beetles were 67.3, 31.4, 19.4 and 15.3 days, respectively. The period of each stage was long in the order of pupa> egg > 3rd larva> 2nd larva> 1st larva. Mean numbers of eggs oviposited by a female per day were 3.3, 5.6, 7.3 and 7.7 under the temperatures, respectively. The highest hatchability (92%) and adult emergence (60%) were shown under 25$\^{C}$. Adult longevity was 54.3 days for females, and 58.6 days for males. In natural condition, the sex ratio of the beetle was 0.58. Developmental thresholds (DT) for egg, 1st, 2nd, 3rd larva, pupa, and egg to adult were 12.7, 10.0, 10.8, 7.9, 10.1 and 10.6$\^{C}$, respectively. The effective cumulative temperatures (ET) were 41.4, 22.9, 22.7, 46.6, 165.3, 292.9 day-degree at the same development stage, respectively. The beetle occurred from early April to early December in yuzu orchards. The population peak of the beetles was dependent on the amount of prey mite (Panonychus citri), and the ratio of the predator to prey mite was 1 to 100-200 at predator peak stage in the field.

The Stability Analysis Method with the Failure Shape in Cutting Slopes (절취사면에서의 파괴형태에 따른 안정해석방법)

  • Kang, Yea Mook;Chee, In Taeg;Kim, Yong Seong;Kim, Ji Hoon
    • Korean Journal of Agricultural Science
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    • 제25권1호
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    • pp.97-106
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    • 1998
  • This study was carried out to investigate the problem of analysis method of circular sliding, which uses a high rate to work out a countermeasure for landslides. The results of this study were summarized as follows : 1. As a result of the analysis of sliding surface along the soil layers in forty model slopes, the boundary layer in weathered soil and weathered rock indicated a very high possibility of sliding than in other places. 2. Because most landslides in Korea occur along the discontinuity face at the boundary of soil layers, below 2m. from land surface, it is a good method for safe design to work the countermeasure for these kinds of landslides in cutting slopes. 3. When the inclination of slopes is fixed and the length of slopes is changed, the cercular sliding slopes were more safe as the soil layers are more shallow and the length of slopes are shorter, but the safety ratio of infinite sliding slopes was same as the other even though their length of slopes was different. 4. As a result of the analysis by cercular sliding analysis method and infinite sliding analysis method with some condition that the inclination of slopes was $30^{\circ}$ degree, because most landslides in Korea occur at this condition, these methods indicated different results to each other as well as cercular sliding analysis method showed too much safety ratio than infinite sliding analysis method.

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Development by Temperatures and Copulation Flight of Comstock mealybug, Pseudococcus comstocki (가루깍지벌레(Pseudococcus comstocki )의 온도별 발육 및 교미비행)

  • Cho, Min-Kyu;Kwon, Hey Ri;Yu, Yong Man;Youn, Young Nam
    • Korean journal of applied entomology
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    • 제57권2호
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    • pp.105-115
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    • 2018
  • The comstock mealybug is one of the serious insect pest in pear. It is necessary effective control methods such as attractive sex pheromone. Using sex pheromones is necessary for studying the physiology and ecology of insect pests. In the present study, the sex pheromone of comstock mealybug was used for investigating the flying acts of the adult male. As temperature increased, the development period of eggs shortened, but it was longer at $30^{\circ}C$. The mean number of eggs laid was 482 at $25^{\circ}C$ and males constituted 37% of the experimental population at $30^{\circ}C$. The flight of the male comstock mealybug was mostly restricted to a 2-4 hour period after sunrise, even if condition of light period is changed. Male mealybugs were incapable of upwind flight towards an attractant when air velocity was 1.5 mph. Preference of height was tested in a pear orchard. The ineffective heights of the traps were 2 and 2.5 m, whereas the effective heights were 1 and 1.5 m. Many captured males at 0.5, 1, and 5 m, and the number of captured males were similar in 10, 15, and 20 m. Male mealybugs were captured up to a distance of 50 m, suggesting that it is possible to control the male mealybugs that are more than 50 m away when sex pheromone traps are used.

Application of the SASW Method to the Evaluation of Grouting Performance for a Soft Ground of a Tunnel (터널 원지반의 그라우팅 보강 평가를 위한 SASW 기법의 적용)

  • 조미라;강태호
    • Journal of the Korean Geotechnical Society
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    • 제19권6호
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    • pp.273-283
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    • 2003
  • Fissured rock and soft ground always suggest, problems in the construction of the underground space. The stress release of the weak underground material by opening the underground space with a soft ground, fissures and joints can lead to the failure of the opening. Grouting of the weak rock and the soft ground, which is a process of injecting some bonding agents into the soft ground, is one of the measures to reinforce the soft ground and to prohibit the failure of the underground construction due to the stress release. The proper installation of the grouting is essential to ensuring the safety of the tunneling operation, so that the evaluation of the grouting performance is very significant. The general procedure of evaluating the grouting is coring the grouted section and measuring the compression strength of the core. However, sometimes when the grouted section is at the crown of the tunnel and the grouting is installed at a wide section, the coring is not good enough. This study is oriented to propose a new and a non-destructive procedure of evaluating the grouting performance. The proposed method is based on the wave propagation of elastic waves, and evaluates the shear stiffness of the ground and investigates the anomalies such as voids and cracks. The SASW ( Spectral-Analysis-of-Surface-Waves) method is one of the candidate s to make the inspection of the pouting performance, and is adopted in this study. The practical grouting activity was monitored by SASW method, and the proposed method was applied to the inspection of the grouting performance to check the verification of the proposed method.

K-Ar Ages of the Volcanic Rocks from the Cretaceous Strata in Gurye Area, Jeonnam Province, South Korea (전남 구례지역의 백악기층에 나타나는 화산암에 대한 K-Ar 연대)

  • Park, Ju-Hyun;Park, Da-Hyun;Won, Beom-Hee;Kang, Sung-Seung;Kim, Cheong-Bin
    • Journal of the Korean earth science society
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    • 제36권1호
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    • pp.27-35
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    • 2015
  • This study aims to establish the age of the Cretaceous Togeum Formation in Gurye that reported the discovery of dinosaur eggshells and bones. This study also investigates to determine the period of the dinosaurs' dominance in the region. K-Ar ages are measured on the whole volcanic rocks in the lower - and upper parts of the formation. The six samples dated are volcanic pebbles deposited in the Geumjeongri Conglomerate that is distributed underneath the Togeum Formation, and the ages dated as Aptian ($118.3{\pm}2.3Ma$) or Albian ($103.6{\pm}2.0$, $102.5{\pm}2.0$, $99.9{\pm}1.9Ma$), which all correspond to the Early Cretaceous. In addition, the ages of andesites and porphyritic andesites overlying the Togeum Formation are dated in a similar way. The result is that the ages are apparently corresponding to the Campanian ($83.9{\pm}1.6$, $74.2{\pm}1.5$Ma) of the Late Cretaceous. Field evidence and the age results indicate that the formation of the Togeum and the activities of dinosaurs dated back between 84 and 100Ma. It suggests that the Togeum Formation be somewhat older than the Boseong Seonso Formation (81Ma) which contains egg shells, the Haenam Uhangni Formation (79-81 Ma) that has dinosaur, pterosaur and webbed bird footprints, and also older than the theropod egg nests (77-83Ma) found in the Aphaedo area.

The effect of irradiation and pH on sporulation and growth of Piricularia oryzae CAV. on tomato juice media (Tomato 즙배지의 pH와 조사광선이 도열병균 분생포자생성 및 균사생장에 미치는 영향)

  • Oh S. H.;Cho Y. S.;Lee S. C.
    • Korean journal of applied entomology
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    • 제4권
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    • pp.19-24
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    • 1965
  • In an attempt to find a satisfactory environmental factors which facilitate abundant conidial production of Piriculariaoryzae Cav. on tomato juice media, various environmental factors were studied for their effect on sporulation and mycelial growth of the fungus. Those factors were conditions of irradiation, color of light, age of culture and pH of the media. l) Continuous exposure to fluorescent light (Mitsubish FL-20-35 W) produced more conidia and much mycelial growth than did intermittent photoperiods and darkness. 2) Of 3 cellophane filters and direct exposure to fluorescent light used, conidia were produced best under the direct exposure to the light. Conidial production in color filter conditions sequently decreased with red, yellow and blue. Growth of mycelium was not significantly different within colors. 3) Periodic irradiation of 12-hour unit brought about zones on mycelial growth no matter what the color filter was used. 4) Older cultures responding to the light were more stimulated by light than were the younger one in the conidia production, but maximum production of conidia was 48 hours of age in this case. 5) Color of the mycelial mat and the aerial mycelium seemed to have a close relation to the production of conidia. The more darkness of the mycelial mat was produced the more conidia and the much aerial mycelium was produced the least conidia. The color of mycelium was more dark under the continuous irradiation than continuous darkness, while the periodic irradiation showed intermediate effect. 6) The concentration of hydrogen ion for growth and sporulation of the fungus was investigated the ranges between 5 and 9. The best pH for the fungus was also noted at 7. whereas the below of pH 4 was not occurred any mycelial growth and sporulation.

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The Impact of Tissue Inhomogeneity Corrections in the Treatment of Prostate Cancer with Intensity-Modulated Radiation Therapy (전립선암의 세기조절 방사선 치료시 밀도보정의 효과)

  • Han Youngyih;Park Won;Huh Seung Jae
    • Progress in Medical Physics
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    • 제15권3호
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    • pp.149-155
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    • 2004
  • Purpose: To investigate the effects of tissue inhomogeneity corrections on the dose delivered to prostate cancer patients treated with Intensity-Modulated Radiation Therapy (IMRT). Methods and Materials: For five prostate cancer patients, IMRT treatment plans were generated using 6 MV or 10 MV X-rays. In each plan, seven equally spaced ports of photon beams were directed to the isocenter, neglecting the tissue heterogeneity in the body. The dose at the isocenter, mean dose, maximum dose, minimum dose and volume that received more than 95% of the isocenter dose in the planning target volume ( $V_{p>95%}$) were measured. The maximum doses to the rectum and the bladder, and the volumes that received more than 50, 75 and 90% of the prescribed dose were measured. Treatment plans were then recomputed using tissue inhomogeneity correction maintaining the intensity profiles and monitor units of each port. The prescription point dose and other dosimetric parameters were remeasured. Results: The inhomogeneity correction reduced the prescription point dose by an average 4.9 and 4.0% with 6 and 10 MV X-rays, respectively. The average reductions of the $V_{p>95%}$ were 0.8 and 0.9% with the 6 and 10 MV X-rays, respectively. The mean doses in the PTV were reduced by an average of 4.2 and 3.4% with the 6 and 10 MV X-rays, respectively. The irradiated volume parameters in the rectum and bladder were less decreased; less than 2.1 % (1.2%) of the reduction in the rectum (bladder). The average reductions in the mean dose were 1.0 and 0.5% in the rectum and bladder, respectively. Conclusions: Neglect of tissue inhomogeneity in the IMRT treatment of prostate cancer gives rise to a notable overestimation of the dose delivered to the target, whereas the impact of tissue inhomogeneity correction to the surrounding critical organs is less significant.

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Quality Characteristics of Mulberry Fruit Seolgidduk Added with Citric Acid (구연산을 첨가한 오디 설기떡의 품질 특성)

  • 홍정희;안상희;김미지;박금순;최상원;이순재
    • Korean journal of food and cookery science
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    • 제19권6호
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    • pp.777-782
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    • 2003
  • This study was carried out to investigate the characteristics of mulberry fruit seolgidduk. Four percent additions of mulberry were evaluated as the most suitable concentration for seolgidduk, in order to improve the color of gum metal gray and sour smell. Therefore, in order to improve the preference, citric acid was added. The acidity of mulberry fruit seolgidduk increased and its pH decreased significantly by increasing the amount of citric acid. The moisture content of mulberry fruit seolgidduk showed no significant difference between the groups with added citric acid and the control group. The L value decreased as the citric acid concentration increased. Color a and b (Ed-define 'a' and 'b') values increased significantly by increasing the mont of citric acid. The hardness, cohesiveness, springiness, gumminess and brittleness decreased as the citric acid was added. From the sensory evaluation, mulberry fruit seolgidduk with 0.01% citric acid added was the most preferred in taste, chewiness and overall acceptability. These results indicated that mulberry fruit stolgidduk with 0.01% citric acid added showed the best quality.