• Food Science and Preservation
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• v.21 no.6
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• pp.844-850
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• 2014

This study was conducted to examine the physiological activities of Lespedeza cundata extracts. The extraction yield of 50% ethanol extract (17.60%) was higher than that of hot water extract (12.60%). The total phenolic and total flavonoid contents of the 50% ethanol extract were 242.26 mg/g and 160.73 mg/g, respectively. The DPPH radical scavenging activities of the hot water and 50% ethanol extracts were 92.07% and 96.38%, respectively. The superoxide radical scavenging activities of hot water and 50% ethanol extracts on $250{\sim}1,000{\mu}g/mL$ were 54.89~85.68% and 44.50~94.46%, respectively. The tyrosinase inhibition activity of the 50% ethanol extract at $1,000{\mu}g/mL$ (63.31%) was the highest. The nitrite scavenging activity of the 50% ethanol extract was higher than that of the hot water extract. The nitric oxide production of 50% ethanol extract ($7.15{\sim}20.61{\mu}M$) improved with an increase in the treatment concentration. The hot water and 50% ethanol extracts at $1,000{\mu}g/mL$ inhibited the proliferation of the cancer cell lines A549, HeLa, Hep3B, and Sarcoma180. There results suggest that the 50% ethanol Lespedeza cuneata extracts may be useful as a functional food material in the food industry.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.18 no.2_spc
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• pp.305-315
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• 2020

For geological disposal of radioactive wastes, a method was proposed to evaluate the radionuclide transport in the biosphere by calculating the elapsed time of nuclide migration. The radionuclides were supposed to be introduced from a natural barrier and reached a large surface water body following a groundwater flow in a shallow subsurface. The biosphere was defined as a shallow subsurface environment that included aquifers on a host rock. Using the proposed method, a calculation algorithm was established, and a computer code that implemented the algorithm was developed. The developed code was verified by comparing the simulation results of the simple cases with the results of the analytical solution and a public program, which has been widely used to evaluate the radiation dose using the radionuclide transport near the surface. A case study was constructed using the previous research for radionuclide transport from the hypothetical geological disposal repository. In the case study, the code calculated the mass discharge rate of radionuclide to a stream in the biosphere. Because the previous research only demonstrated the transport of radionuclides from the hypothetical repository to the host rock, the developed code in the present study could help identify the total transport of radionuclide along the complete pathway.

• The Journal of Engineering Geology
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• v.15 no.3
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• pp.257-268
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• 2005

In situ rock mass is generally heterogeneous and discontinuous, with varying degrees of strength along the planes of weakness. The planes of weakness such as joints, faults, cracks and bedding planes, control the strength and deformation characteristics of the rock mass. Subsequently, the stability of underground opening depends upon the spatial distribution of discontinuities and their mechanical properties in relation with geometrical shape of openins as well as the mechanical properties of intact rock materials. Understanding the behaviour of a discontinuous rock mass remains a key issue for improving excavation design in hiかy stressed environments. Although recent advances in rock mechanics have provided guidelines for the design of underground opening in isotropic rock mass, prediction and control of deformation in discontinuous rock masses are still unclear. In this study, parametric study was performed to investigate the plastic zone size, stress distribution and deformation behavior around underground opening in a discontinuous rock mass using a continuum joint model. The solutions were obtained by an elasto-plastic finite difference analysis, employing the Mohr-Coulomb failure criteria. Non-associated flow rule and perfectly plastic material behavior are also assumed.

• Journal of Life Science
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• v.18 no.7
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• pp.952-957
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• 2008

We evaluated the effects of various factors (e.g., serum, inhibitors of protein synthesis, and cytoskeleton and protein kinases) on early PMA-induced THP-1 cell adhesion using an adhesion assay with Sulforhodamine B (SRB) staining, which was used to assess the proliferation of the attached cells. THP-1 cell adhesion to a plastic substrate was detected 1 hr after exposure to Phorbol 12-Myristate 13-Acetate (PMA) and peaked after 18 hr. At concentrations > 25 nM PMA, the level of adhesion did not change. Based on our preliminary results, we used 25 nM PMA and 5 hr of culture as standard assay conditions. Early PMA-induced cell adhesion was not affected by the presence of serum or PD 98059 in the culture medium, but was affected by the addition of PKC inhibitors and cycloheximide. In the presence of actin inhibitor with PMA, the cell adhesion increased when comparing with PMA treatment only. Thus, early PMA-induced adhesion of THP-1 cells does not require serum in the culture medium, MAP-kinase activation, or actin polymerization, but does require de novo protein synthesis and PKC activation. Our SRB-based cell adhesion assay may be used to screen other PKC inhibitors.

• Journal of the Korean Geotechnical Society
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• v.15 no.6
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• pp.263-272
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• 1999

Slice method is commonly used in solving slope stability problems since it is easy to use and its computation time is rather short. But depending upon the assumptions on the inter-slice forces, different methods are available. Quite often the difference between methods are so big that it is very difficult to make engineering decisions. This paper describes a method to calculate the factor of safety of a slope using FLAC, a finite difference based program. A FISH routine is developed to calculate the factor of safety, and verified by comparing with Chen's limit equilibrium solution. An example problem was selected from Fredlund and Krhan's paper, and results were compared for different soil and water conditions. The difference was less than 0.01 when the soil is homogeneous, and less than 5 % when a weak layer is embedded. Since most geotechnical application programs are capable of considering complicated ground conditions as well as the effect of ground supports, numerical methods are believed to be very useful in making engineering decisions. The developed routine can be applied to the calculation of the factor of safety of jointed rock slopes or weathered rock slopes where the use of slice method is limited.

• Journal of Life Science
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• v.12 no.6
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• pp.708-714
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• 2002

A new leaf blight was found on the perilla leaves at the major perilla-cultivating areas such as Kangdong in Busan and Miryang in Kyungnam province. Symptoms of the disease initially appeared on the edge of perilla leaves showing black necrosis and drying, and the infected leaves were finally fell down. The SD1 isolate showing strong pathogenicity and forming abundant conidial spores on the diseased lesions was isolated. Among the tested media, mycelial growth was abundant on PDA (Potato Dextrose Agar) medium at $25^{\circ}C$ under dark condition, but conidial formation was greater on V8A (V-8 juice A8ar) medium than that on PDA medium. Optimal temperatures for mycelial growth and conidial formation on PDA medium were respectively $25^{\circ}C$ and 3$0^{\circ}C$. The rate of conidial germination and the elongation of germ tube were more effective in 10% tomato juice than those in PDB (Potato Dextrose Broth) and sterile water. In 10% tomato juice, the rate of conidial germination and the length of germ tube were 100% after incubation for 24k and 535.2${\mu}{\textrm}{m}$ after incubation for 36hr, respectively. According to the result of pathogenicity, it revealed that conidial suspention with 10% tomato juice was the most effective for pathogenicity test showing as 100% of disease incidence, and the symptoms caused by artificial inoculum were same as those of naturally infected perilla. In this study, the SD1 isolate according to the results of morphological characteristics, the incubation characteristics and pathogenicity was firstly identified A. alternata, and named as leaf blight of perilla.

• Analytical Science and Technology
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• v.24 no.6
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• pp.510-518
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• 2011

Recent developments and improvements of multiple technological elements including mass spectrometry (MS) instrument, multi-dimensional chromatographic separation, and software tools processing MS data resulted in benefits of large scale proteomics analysis. However, its throughput is limited by the speed and reproducibility of the protein digestion process. In this study, we demonstrated a new method for rapid proteolytic digestion of proteins using acoustic technology. Tryptic digests of BSA prepared at various conditions by super acoustic for optimization time and intensity were analyzed by LC-MS/MS showed higher sequence coverage in compared with traditional 16 hrs digestion method. The method was applied successfully for complex proteins of a breast cancer cells at 30 min of digestion at intensity 2. This new application reduces time-consuming of sample preparation with better efficiency, even with large amount of proteins, and increases high-throughput process in sample preparation state.

• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.71-75
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• 1997

For the establishment of early selection of heat-tolerant clones through in vitro tuberization of true potato seeds, different temperature treatments for in vitro tuberization were investigated. The ratios of tuberization at 2$0^{\circ}C$ on var. Superior and DTO-33 treated with 5 mg/L of BAP and 500 mg/L of CCC, were 85% and 92%, respectively. At 3$0^{\circ}C$, the ratio of tuberization on DTO-33 was 37%, which revealed strongly heat-tolerant clone. In culture system of in vitro tuberization, the number of tubers per flask at 2$0^{\circ}C$ on non-subculture incubation was more than that on subculture incubation. The condition of non-subculture and short-day treatment for 4 weeks was good for production as 10.6 tubers per flask, which was very similar to that of long-day treatment. On the other hand, tuber diameter on long-day treatment was greater as 11.2 mm than on short-day treatment. Therefore, in vitro tuberization from true potato seeds could be induced under the condition of long-day treatment at darkness.

• Journal of Sericultural and Entomological Science
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• v.51 no.1
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• pp.73-77
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• 2013

Silkworm transgenesis scientists have done some genetic modification work on multivoltine silkworms, but that type of silkworms is less commercial feasible. They are easy to manipulate, because they breed all year round. But the commercial silkworm variety must undergo hydrochloric acid treatment at a high temperature to be artificially hatched. Hydrochloric acid penetrates through the holes in the silkworm eggs, fatally damaging their reproduction. So it had been thought that altering the properties of the commercial silkworm variety would be very difficult. So we have tried to make from diapause to non-diapause eggs using diapauses varieties, 'Backokjam' and 'Jam 124'. At present, our group has establishing the conditions for non-diapause eggs. Oviposited eggs after 40 ~ 60 hours were incubated for 24 hours at $15{\sim}20^{\circ}C$ with dark condition. Non-diapause eggs were completely induced. The hatching rate, molting rate and pupation rate of non-diapause 'Jam 124' and 'Backokjam' eggs showed no differences compared to diapause eggs. When transgenic silkworm using the non-diapause eggs, the hatching rate showed that non-diapause eggs induced from diapause were 40 ~ 70%, diapause eggs treated with artificial incubation were 10 ~ 30%, and polyvoltine strains, HM eggs were 30 ~ 50%. Therefore, we suggest that modification techniques of the commercial silkworm eggs adequate for silkworm transgenesis can be used to develop transgenic silkworms more easily.

• Journal of the Mineralogical Society of Korea
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• v.21 no.2
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• pp.209-224
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• 2008

Various types of high-purity limestone, which occurred in the Pungchon Formation, are examined to understand applied-mineralogical factors controlling their calcination characters with respect to the ore characters. To do this work, systematic characterization and determination were carried out for the limestone ores and their calcination products in a fixed heating condition, and the results were correlated and discussed. During the calcination experiment, a phase transition from calcite to quicklime begins to occur selectively in the physical weak zones such as grain boundary, cleavage and twin planes. All the fabrics of original limestones are preserved in the resultant quicklime. In addition, crystallinity of the quicklime was advanced, as the aging time of calcination was increased. Major controlling factors on the calcination effects of the high-purity limestone are elucidated to be the degree of development of cleavage and twin, together with crystallinity and textures in the limestone ore. Especially, lower crystallinity and dense interlocking fabrics obviously play advantageous role in all the calcination characters. But the development of cleavage and twin affects negatively on the calcination characters on account of favoring decrepitaion of quicklime in the lime manufacturing. Thus, the high-purity limestones characteristic of marble fabrics and relatively lower crystallinity are comparatively advantageous for the uses of lime manufacture.