• Korean Journal of Soil Science and Fertilizer
• /
• v.35 no.1
• /
• pp.38-46
• /
• 2002

Chrysanthemum boreale M. (hereafter, C. boreale M.), a perennial flower, has been historically used as a natural medicine in Korea. With increasing concerns for health-improving foods, the demand for C. boreale M. has become higher than ever. Howevr, the amount of wild C. boreale M. collected from mountainous areas is not enough to cover all demands. The cultivation system and fertilization strategy are required to meet increasing demand on C. boreale M. with a good quality. We investigated the effects of nitrogen application on plant growth and effective components of C. boreale M. to suggest optimum rate of nitrogen fertilization. C. boreale M. was cultivated in a pot scale (1/2000a scale), and nitrogen applied with rate of 0(N0), 50(N50), 100(N100), 150(N150), 200(N200), and $250(N250)kg\;ha^{-1}$. Phosphate and potassium were applied at the same level ($P_2O_5-K_2O=80-80kg\;ha^{-1}$) in all treatments. Maximum yield achieved in 246 and $226kg\;ha^{-1}$ N treatment on the whole plant and the flower part, a valuable part as a herbal medicine, respectively. Proline was the most abundant amino acid in the flower of C boreal M. and the contents of amino acids increased with increasing nitrogen application rate in flower. Nitrogen recovery efficiency was high more than 41% in all nitrogen treatments and increased to 61.8% in nitrogen N100 treatment. From the nitrogen content, the high nitrogen uptake, the low residue of mineral N and the reasonably good apparent fertilizer recovery, it can be inferred that C. boreale M. made efficient use of the available nitrogen. In flower, contents of Cumambrin A. which is a sesquiterpene compound and has the effect of blood-pressure reduction, decreased with increasing nitrogen application. However, the amount of Cumambrin A in flower increased as nitrogen rate increased, because of increasing flower yield. Conclusively, nitrogen fertilization could increase yields and enhance quality. The optimum nitrogen application rate might be on the range of $225{\sim}250kg\;ha^{-1}$ in a mountainous soil.

• Korean Journal of Soil Science and Fertilizer
• /
• v.31 no.3
• /
• pp.238-245
• /
• 1998

The reasonable level of nitrogen fertilizer is a key factor to reduce environmental contamination as well as to increase crop yield and quality. The treatments are $N=42kg\;10a^{-1}$, $N=48kg\;10a^{-1}$, $N=54kg\;10a^{-1}$. $N=60kg\;10a^{-1}$. $N=72kg\;10a^{-1}$ and $N=150kg\;10a^{-1}$. Analytical results of yield and quality of tea leaves harvested the second time are summarized as follows: The soil of experimental field was higher in organic matter($49.9g\;kg^{-1}$) and available phosphate($937mg\;kg^{-1}$) compared to the general field but lower in pH(5.07) compared to the general field. When the amount of nitrogen fertilizer application was raised, the yield of tea leaves, the content of nitrogen, total free amino acids, caffeine and chlorophyll increased: however, the yield of tea leaves increased to excessive levels. On the other hand, the increased level of nitrogen fertilizer did not show any difference in the contents of vitamin C and tannin. Eleven kinds of amino acids were isolated from second harvested leaves of tea where the content of theanine occupied over 50%, and content of $665{\sim}763mg\;100g^{-1}$. The contents of fatty acids and catechin did not show correlation with application level of nitrogen fertilizer. The content of fatty acid was produced $1,742{\sim}2,643mg\;100g^{-1}$, and content of catechin was produced 12.47~13.54%. In scoring test, $N=60kg\;10a^{-1}$ treatment was 0.5~6.0 point higher compared to other treatments. Consequently, $N=60kg\;10a^{-1}$ is considered to be the best level of nitrogen fertilizer in terms of decreased environmental contamination as well as to increase crop yield and quality.

• Korean Journal of Soil Science and Fertilizer
• /
• v.16 no.3
• /
• pp.274-279
• /
• 1983

In order to find out the application effect of commercial organic fertilizer on the germination rate of ginseng seed, growth of ginseng plant and changes of the physicochemical properties of soils, a Miwon organic fertilizer, byproduct of amino acid fermentation, was applied to the ginseng nursery bed. The application rates of Miwon organic fertilizer were 0kg-N from organic fertilizer and 30kg-N from wild grass compost per 10a as control(namely 0kg-N/10a), 2.5kg-N and 27.5kg-N/ 10a(2.5kg-N/10a) 5.0kg-N and 25kg-N/10a(5.0kg-N/10a), 10kg-N and 20kg-N/10a(10kg-N/10a), 15kg-N and 15kg-N/10a(15kg-N/10a) respectively, The obtained results are as follows: 1. The germination rate of ginseng seed were better in the plots that received 2.5kg, 5.0kg and 10kg-N/10a as compared with control plot. However no difference was found on germination rate between control and 15kg-N/10a, 2. The higher rate of missing plant was found in the 15kg-N/10a plot that showed poor growth. However control and 10kg-N/10a plot showed similar rate of missing plant. The best result, lower rate of missing plant, was obtained in the order of 5kg-N and 2.5kg-N/10a respectively. 3. The significant heavier ginseng root weight was obtained in 5kg-N/10a and 10kg-N/10a plot. However the root weight was decreased in the order of 2.5kg-N/10a, control, 15kg-N/10a. 4. In the physicochemical changes of soils between control and organic fertilizer treated soil before and after harvesting the increment of pH, C.E.C. and organic matter content along with the rate of organic fertilizer application were found. There were significant correlation between nitrate nitrogen and the rate of organic fertilizer application. 5. In the view points of germination rate, missing rate of plant and yield of ginseng root, it can be stated that the best result could be obtained from the plot that received 10kg-N/10a from Miwon commercial organic fertilizer and 10kg-N/10a from wild grass compost.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.5 no.2
• /
• pp.47-56
• /
• 1997

To examine of possibility protein recycling of shaving scraps contained chrome generated from manufacturing process of leather, the characteristics of hydrolyzed protein that differently treated with MgO as alkaline agent were investigated. In alkaline hydrolysis of saving scraps treated with MgO, MgO had to be treated over 5.0% to maintain over pH 8.0 that is insoluble of chrome. Under the condition of alkaline treated with MgO, the solubility of chrome is low with about 60%. The average molecular weight of hydrolyzed proteins from shaving scraps treated with MgO was about 80~100 KD. The amino acid contents of that were largely collagen proteins such as glycine, alanine and proline, and acidic amino acids such as aspartic acid and glutatamic acid. The contents of Mg, Ca and Na in hydrolyzed protein were too much as liquid fertilizer, and chrome contents was 30~40 ppm that largely decreased in comparing with raw materials (40,000~42,000 ppm).

• Korean Journal of Soil Science and Fertilizer
• /
• v.3 no.1
• /
• pp.49-54
• /
• 1970

In an effort of determine the metabolism and bio-synthesis of nitrogen, was studied at variance of souble nitrogens, free amino acids and total alcohol soluble amino acids during the growth of younger soybean plants, and saybean divides into cotyledon and embryonic organ (shoot and root system) in this experiment. 1. In the soluble nitrogen of soybean, ratio of increase and decrease in the amino acids of them was displayed the near phenomena both cotyledon and embryonic organ of soybean. But, in the 17th days after seed germination, that is the developmeatal stage of adult leaf of soybeans, was appear the maximum value. It has been suggested that the stage of first half period of growth as boundary the stage of adult leaf development which indicated clear morphologically, at the younger soybean plants, is the step that nitrogen assimilation. 2. It was investigated the amino acids of seventeen kinds in the seed state, but at the third days after seed germination, was investigated the amino acids of nineteen kinds. Ultimately, it appears the translocation from cotyledon to embryonic organ in the distribution of amino acid, the nineth day which differentiation begining day of embryonic organ, then after, it happen the variation of number of inspected amino acid in the cotyledon and embryonic organ but only the variance changes in the distribution and quantitative aspects. Especially, the most conspicuous fact was indicated the accumulation of Asparagine, that is the phenomena of Asparagine-accumulation was constitute, not with standing no fertilization from outside. It may be concluded from the results of this investigation that the difference of special phenomena of soybean from the embryo of other plans. 3. In the initial stage of differentiation at embryonic organ number of inspected amino acid was very few, and then, it was slightly appeared the increase-phenomena in the number of them. It was that the amino acid inspecting the initial stage was translocated from the cotyledon. It is suggested that the intermediate-metabolism of amino acid was constituted on the basis of above the result. 4. The phenomena of increase and decrease of total alcohol soluble amino acid were essentially identical to the water soluble amino acid of soybean, but it was appeared the severe difference of amounts in both of them.

• Korean Journal of Soil Science and Fertilizer
• /
• v.17 no.4
• /
• pp.356-362
• /
• 1984

A field experiment was carried out to investigate the sulfur effects on yield, growth and nutritive qualities of soybean in the different fertilizer application methods. Ammonium sulfate and super-phosphate were applied to the soil with levels of 0, 2, 4, and 6Kg S/10a and potassium sulfate, ammonium sulfate and thiourea were applied by foliar application with 2KgS/10a. The results obtained were as follows; 1. Grain and dry matter yield of soybean were increased by the increase of sulfur application and sulfur application showed positive effect on yield components. 2. Sulfur application increased the sulfur content and decreased N/S ratio in grain of soybean. 3. Protein content was increased by raising sulfur application up to 4Kg S/10a. Sulfur application influenced on amino acid composition of soybean protein. Cysteine and methionine contents were increased by sulfur application. 4. Lipid content of soybean grain and inorganic ion absorption by soybean plant were not influenced significantly by sulfur application. 5. In application effects, there was no significant difference among sulfur fertilizers, but foliar application was more effective than soil application.

• Korean Journal of Soil Science and Fertilizer
• /
• v.23 no.3
• /
• pp.214-219
• /
• 1990

This study was conducted to characterize red worm(Lumbricus rubellus) and its cast reared six months using with pulp sludge, mixture of pulp sludge and methane sludge, and cow manure and methane sludge. The results obtained were as follows: 1. The optimum growth temperature ranged from $16^{\circ}C$ to $25^{\circ}C$ and monthly weight increase was about one kilogram per square meter. 2. The weight of red worm increased 9 to 11 percent reared with mixture of cow manure and methane sludge compared to pulp sludge. 3. Red worm contained large amount of amino acid, including. Lycine, which, might be a good source for a feed additive. 4. Yields of red worm cast ranged from 90 to 95 ton/10a/year on dry weight base. 5. CEC of red worm cast varied from 19.4 to 49.9 meg/100g and O.M content ranged from 26.4 to 35.1 percent. It contained lots of nutrients resulting in a good fertilizer source.

• Korean Journal of Soil Science and Fertilizer
• /
• v.45 no.5
• /
• pp.760-763
• /
• 2012

This study was conducted to investigate foliar treatment effects of organic agricultural materials for the environmentally-friendly cultivation of grape. The organic materials applied were chitosan, wood vinegar (pyroligneous acid), amino acid solution, and ginkgo leaf extract. All the organic materials were relatively strong acidic ranging lower than pH 4.6. when comparing with other organic materials, amino acid solution contained relatively high contents of selected plant nutrients, such as N, P, K, Ca, Mg, Cu, Fe, Mn, and Zn. As comparison of selected soil properties in the grape cultivating field, soil pH values were lower at the harvest stage than at the first stage of grape growing (before treating the organic materials), and electrical conductivity (EC) and soil organic matter content were higher at the harvest stage in the all plots. The concentrations of available phosphorus increased in most of the plot soils except in control plot (conventional treatment). The concentrations of exchangeable K decreased in the plot treated with ginkgo leaf extract and the control plot. The exchangeable Mg concentrations decreased in soils of all the plots. On the other hand, the concentrations of N and K in the grape leaves were higher with the treatments of chitosan and amino acid solution, P concentrations were higher with the applications of chitosan, wood vinegar and amino acid solution, and Ca and Mg concentrations were higher with chitosan and amino acid solution treatments, respectively, than with others. The yields of grape were higher, $1,581{\sim}1,583kg\;10a^{-1}$, in the control and wood vinegar treatment plots than others. Sugar contents of grape were not different among all the plots.

• Weed & Turfgrass Science
• /
• v.3 no.1
• /
• pp.41-49
• /
• 2014

Four preparations of liquid turfgrass fertilizers were tested cool season turfgrasses during fall season. The preparations of PS-A, PS-B, PH-C and PH-D for commercial products were contained with essential nutrient elements, seaweed extract, amino acids, and humus substance. Growth and quality of creeping bentgrass (Agrostis palustris Huds cv. Penn-A1) and Kentucky bluegrass (Poa pratensis L.) mixture (Midnight 33%, Moonlight 33%, Prosperity 33%) were evaluated by normalized difference vegetation index (NDVI), root length and fresh weight, shoot density, turf color, and chlorophyll content. Three foliar sprays of 4 preparations with an interval of 7 days were made in the fall of 2013 in the sod production field at Hapchun, Korea. No significant difference among 3 preparations was found in NDVI of creeping bentgrass. However, PS-A was significantly increased NDVI of Kentucky bluegrass. Two to three applications of PS-A significantly increased chlorophyll content and turf color. Three foliar sprays of PS-A and PS-B were significantly increased the shoot density of Kentucky bluegrass on 20 days after final application. These results may indicate that the use of some preparation is beneficial in producing higher quality turfgrass sod with better color and chlorophyll content during fall season.

• Korean Journal of Soil Science and Fertilizer
• /
• v.36 no.3
• /
• pp.134-144
• /
• 2003

A rhizobacterium Pseudomonas cholororaphis O6 produced several secondary metabolites, such as phenazines, protease, and HCN that may be involved in inhibition of the growth of phytopathogenic fungi. In field study, P. chlororaphis O6 treatment on wheat seed suppressed root rot disease caused by Fusarium culmorum. The major organic acids of cucumber root exudates were fumaric acid, malic acid, benzoic acid, and succinic acid. Glucose and fructose were major monosaccharides in cucumber root exudates. The total amount of organic acids was ten times higher than that of the sugars. P. chlororaphis O6 grew well on cucumber root exudates. The dctA gene of P. chlororaphis O6 consisted of a 1,335 bp open reading frame with a deduced amino acid sequence of 444 residues, corresponding to a molecular size of about 47 kD and pI 8.2. The deduced dctA sequence has ten putative transmembrane domains, as expected of a membrane-embedded protein. Our results indicated that organic acids in cucumber root exudates may play an important role in providing nutrient source for root colonization of biological control bacteria, and the dctA gene of P. chlororaphis O6 may be an important bacterial trait that is involved in utilization of root exudates.