• Proceedings of the Korea Information Processing Society Conference
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• 2018.10a
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• pp.603-605
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• 2018

최근 대두되고 있는 딥 러닝은 학습을 통해 사물이나 데이터를 군집화하거나 분류하는 데 사용하는 기술이다. 본 논문은 딥 러닝에 활용하기 위해 개발된 오픈소스 소프트웨어인 텐서플로 Inception V3을 사용해 연구를 진행했다. 딥 러닝을 활용한 씨앗 발아 확인 시스템은 기존의 영상 처리를 활용한 시스템에서 고안했으며, 씨앗 발아 여부의 정확성이 떨어지는 단점을 개선하고, 모든 종자들의 발아 여부를 확인할 수 있도록 구현해 사용자가 효과적으로 연구를 수행할 수 있도록 하는 목적에 있다.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.6
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• pp.396-401
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• 2014

Nanomaterials have been widely used in many fields. This study investigates the effects of metal oxide nanoparticles (CuO, NiO, $TiO_2$, $Fe_2O_3$, $Co_3O_4$, ZnO) on germination and germination index (G.I.) of seeds, Lactuca and Raphanus. Under aqueous exposure, CuO on Lactuca shows the most significant impacts on activities compared to others, showing $EC_{50s}$ for germination and G.I. as 0.46 mg/L and 0.37%, respectively. The effects of nanoparticle phytotoxicity on seed Lactuca was much higher than that of Raphanus. In general, the toxicities on seed germination and germination index were as following orders : CuO > ZnO > NiO ${\gg}$ $TiO_2$, $Fe_2O_3$, $Co_3O_4$. No measurable inhibition was observed at 1,000 mg/L (maximum exposure concentration) of $TiO_2$, $Fe_2O_3$, $Co_3O_4$.

• Applied Biological Chemistry
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• v.39 no.1
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• pp.25-31
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• 1996

Transgenic tobacco plants expressing calmodulin derivative($lys{\rightarrow}ile$ 115 calmodulin) and hygromycin resistance genes or hygromycin resistance gene alone(control) were generated by Agrobacterium-mediated DNA transfer. Seeds obtained from the transgenic plants($F_o$) were tested for resistance to hygromycin and the expected 3 : 1 ratio was observed. The expression of calmodulin derivative in the tobacco plants was identified by a combined method of Western blot and Chemiluminescence. The effects of surface sterilizers on the germiation of seeds from transgenic tobacco plants were tested in Murashige and Skoog agar medium. Seeds obtained from transgenic tobacoo plants expressing the calmodulin derivative showed no fungi contamination with normal germination by treating with sterilized water alone or sodium hypochlorite(2% effective chlorine). In contrast, seeds from the control transgenic tobacco plants showed severe contamination with fungi by treating with sterilized water alone and showed no contamination with normal germination by treating with sodium hypochlorite(2% chlorine). The effects of calcium concentration on the germination of seeds from transgenic tobacco plants were tested in Murashige and Skoog agar medium. Seeds obtained from transgenic tobacco plants expressing the calmodulin derivative showed better germination frequency than that of the control transgenic tobacco seeds in the medium containing 30 mM $CaCl_2$. The data raise the possibility that the expression of calmodulin derivative gene in tobacco plants could increase adaptability of the seeds to environmental stresses.

• Clean Technology
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• v.21 no.1
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• pp.39-44
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• 2015

This study investigated the effects of six metal oxide nanoparticles (NPs: CuO, NiO, TiO₂, Fe₂O₃, Co₃O₄, ZnO) on seed germination and germination index (G.I) for five types of seeds: Brassica napus L., Malva verticillata L., Brassica olercea L., Brassica campestris L., Daucus carota L. NPs of CuO, ZnO, NiO show significant toxicity impacts on seed activities [CuO (6-27 mg/L), ZnO (16-86 mg/L), NiO (48-112 mg/L)], while no significant effects were observed at > 1000 mg/L of TiO₂, Fe₂O₃, Co₃O₄. Tested five types of seed showed different sensitivities on seed germination and root activity, especially on NPs of CuO, ZnO, NiO. Malva verticillata L. seed was highly sensitive to toxic metal oxide NPs and showed following EC₅₀s : CuO 5.5 mg/L, ZnO 16.4 mg/L, NiO 53.4 mg/L. Mostly following order of toxicity was observed, CuO > ZnO > NiO > Fe₂O₃ ≈ Co₃O₄ ≈ TiO₂, where slightly different toxicity order was observed for carrot, showing CuO > NiO ≈ ZnO > Fe₂O₃ ≈ Co₃O₄ ≈ TiO₂.

• Proceedings of the Korea Information Processing Society Conference
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• 2018.05a
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• pp.212-214
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• 2018

식물 연구 분야에서 진행하는 씨앗 발아 확인실험에 대한 사용자의 편의성을 증대시키기 위한 씨앗발아 확인 시스템을 구현하는데 목적이 있다. 본 시스템을 구현 하여 기존 수작업으로 진행하던 실험을 자동화하여 인력 및 시간 소모를 줄여 작업 능률 개선 측면에서 기대효과가 있다.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.8
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• pp.795-801
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• 2010

The acute toxicity of arsenic compounds was assessed and compared using following four bioassays; bioluminescence activity of the recombinant strain RB1436, germination of four different seeds, ${\alpha}$-glucosidase activity produced by Bacillus lichemiformis, acute genetic revertant mutation using mutant strain Salmonella typhimurium. Different sensitivities were observed among tested bioassays, but generally the toxicity by arsenite was greater than that of arsenate. Among tested four seeds, sensitivities of Lactucus and Raphanus were greater than others, and these two seed types were appeared as proper type for bioassay. High revertant mutation ratio (5.1) was observed with 1 mg/L arsenite, indicating high mutagenicity. The sensitivity of ${\alpha}$-glucosidase activity on arsenic compounds was much lower than other methods. The evaluation of interactive toxic effects using various bioassays may comprise a useful tool for the bioassessment of environmental pollutants.

• Economic and Environmental Geology
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• v.49 no.5
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• pp.411-415
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• 2016

In this preliminary study, we assessed the toxicity of domestic bentonites using environmental toxicity testings of seed germination and bacterial bioluminescence. In cases of tested bulk bentonite samples, following ranges of toxicity were observed: -3 ~ 95% bioluminescence, 7.1 ~ 92.1% Lactuca germination. Similar results were also observed among tested other seeds, except seed Raphanus. No significant correlations between toxicity and metals contents of bentonite extractants were observed throughout this investigation. Interestingly, distinct different effects were observed according to bentonite colors (blue $67{\pm}8.7%$, pink $27{\pm}10.4%$, brown $58{\pm}14.5%$).

• Economic and Environmental Geology
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• v.48 no.3
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• pp.261-271
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• 2015

Toxicity results of metals, nanoparticles (NPs), and soils contaminated with metals were introduced on this review. Following methods were used: seed germination, bioluminescence, enzyme activity, and mutation. In general, different sensitivities were observed, depending on types of bioassays and pollutants. Among tested seeds, sensitivities of Lactucus and Raphanus were greater than others. Of single metal exposure, effect by As(III) was greater than others, and high revertant mutation ratio (5.1) was observed at 1 mg/L arsenite, indicating high mutagenicity. No general pattern was observed on the effect of metal mixture, but synergistic effect was observed with seeds. In case of soils, no correlation was observed between total metal contents and toxicity. Toxicity of NPs was observed as follows: CuO > ZnO > NiO > $TiO_2$, $Fe_2O_3$, $Co_3O_4$. Especially, no considerable effects were observed by $TiO_2$, $Fe_2O_3$, and $Co_3O_4$ under tested concentration (max. 1,000 mg/L). The evaluation results of interactive toxic effects using various bioassays may comprise a useful tool for the bioassessment of various environmental pollutants.

• The Microorganisms and Industry
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• v.19 no.3
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• pp.38-40
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• 1993

효소는 일반적으로 건전한 생체에서는 원형질에서 충분히 생성되어진다. 또한 미생물의 배양조건을 달리하던가 씨앗의 발아 시기와 같이 생체가 특수한 상황일 때에는 급격히 증가하기도 한다. 이러한 효소는 주로 단백질로 구성되어 있으며, 그 대부분의 아미노산이 peptide 결합을 하고 있는 고분자 화합물로서 효소의 종류에 따라 특정한 반응에만 특이적으로 작용하는 기질특이성을 가지고 있다. 효소를 이용한 건강식품으로는 체내 과산화지질의 분해를 촉진하는 SOD(superoxide dismutase), 활성 산소나 과산화수소 등의 체외 배출을 돕는 글루타치온 생성효소를 이용한 제품 외에 야채의 미생물 발효를 통하여 미생물 자체나 효소를 이용하는 과채발효 음료 등이 있지만, 여기에서는 특별히 미생물 유래의 효소를 이용한 분말형 효소 제품을 소개하고자 한다.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.200-201
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• 2000

와편모조류의 대부분은 생존에 불리한 환경조건이 되면 저질에 침적되어 휴면포자의 형태로 휴면시기를 보내다가 온도, 광, 산소, 영양염 등의 환경요인 변동과 내재적 주기에 의해 발아가 유도된다. 이러한 휴면포자는 "개체군 씨앗 "으로서 지역적 적조발생의 잠재력이 되는 것으로 시사되어 왔다. (중략)어 왔다. (중략)