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Bioassessment and Comparison of Toxicity of Arsenics based on the Results of Various Bioassays  

Kong, In-Chul (Department of Environmental Engineerting, Yeungnam University)
Kwon, Hyo-Jung (Department of Environmental Engineerting, Yeungnam University)
Ko, Kyung-Seok (Geologic Environment Division, Korea Institute of Geoscience & Mineral Resources (KIGAM))
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Abstract
The acute toxicity of arsenic compounds was assessed and compared using following four bioassays; bioluminescence activity of the recombinant strain RB1436, germination of four different seeds, ${\alpha}$-glucosidase activity produced by Bacillus lichemiformis, acute genetic revertant mutation using mutant strain Salmonella typhimurium. Different sensitivities were observed among tested bioassays, but generally the toxicity by arsenite was greater than that of arsenate. Among tested four seeds, sensitivities of Lactucus and Raphanus were greater than others, and these two seed types were appeared as proper type for bioassay. High revertant mutation ratio (5.1) was observed with 1 mg/L arsenite, indicating high mutagenicity. The sensitivity of ${\alpha}$-glucosidase activity on arsenic compounds was much lower than other methods. The evaluation of interactive toxic effects using various bioassays may comprise a useful tool for the bioassessment of environmental pollutants.
Keywords
Bioassay; Bioluminescence; Revertant Mutation; Enzyme Activity; Seed Germination;
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