• Title/Summary/Keyword: 실험실

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Isoflavone Contents in Some Varieties of Soybean and on Processing Conditions (콩의 종류와 가공 조건에 따른 isoflavone의 함량 변화)

  • 문보경;전기숙;황인경
    • Korean journal of food and cookery science
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    • v.12 no.4
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    • pp.527-534
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    • 1996
  • enistein (G), and daidzein (D), the major isoflavones, were analyzed in 14 varieties of Korean soybean and various processed soybean products by using high performance liquid chromatography. Isoflavone contents (G+D) were greatly variable among varieties ranged from 308.2 $\mu\textrm{g}$/g to 1,134.2 $\mu\textrm{g}$/g and highest in Danyopkong and Jinpumkong. Among hypocotyl, cotyledon and hull of soybean the concentration of the isoflavone (G+D) in the hypocotyl was highest ranged from 2,971.7 $\mu\textrm{g}$/g to 5,704.9 $\mu\textrm{g}$/g. The distributions of genistein and daidzein were also different in hypocotyl, cotyledon and hull. Higher ratio of daidzein to genistein (D/G) was found in the hypocotyl (4-12) compared to cotyledon and hull (0.1-4). Isoflavone (G+D) contents of soymilks (Sinpaldal#2, Eunhakong) prepared at 16 hour hydration were decreased to 1.1-1.2 times compared with that at 8 hour hydration. Commercial soymilks contained much lower isoflavone (G+D) than laboratory soymilks. Soybean curd (Eunhakong) prepared with MgCl$_2$ showed higher isoflavone (G+D) contents than that with CaSO$_4$. But these values of two different soybean curds made at laboratory were similar to those of 3 commercial curds. The concentration of the isoflavones in soybean sprout separated with 3 parts revealed highest in the head and lowest in the stem. Compared with non-fermented soybean foods the fermented soybean produfts, Kochujang and soybean paste, Duen Jang, showed very low contents of isoflavone (G+D),2.8-3.0 $\mu\textrm{g}$/g, 35.9-63.6 $\mu\textrm{g}$/grespectively.

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Effect of Coagulants and Separation Methods on Algal Removal in Water Treatment Process (정수처리에서 응집제 종류와 분리공정이 조류 제거에 미치는 영향)

  • Park, Hung-Suck;Lee, Sang-Yoon
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.2
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    • pp.279-289
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    • 2000
  • The objective of this study was to investigate the effect of coagulants and solid-liquid separation methods on algal removal in water treatment processes. Thus characterization of raw water quality in terms of turbidity. UV-254, $KMnO_4$ consumption, chlorophyll-a and correlation analysis of these parameters were conducted. In addition, the effect of commercial Al-based coagulants(Alum. PAC and PACS) on algal removal was studied by turbidity and organic removal, algal species removal, characteristic of pH drop and alkalinity consumption using laboratory jar tests. Organic components including UV-254, $KMnO_4$ consumption, chlorophyll-a in case of algal bloom were highly correlated with turbidity and the correlation coefficients of UV-254, $KMnO_4$ consumption, chlorophyll-a with turbidity were 0.775, 0674 and 0.623, respectively. In coagulation and sedimentation, the Al-based coagulants showed similar efficiency of organic and turbidity removal in low organic($KMnO_4$ consumption below 15mg/l) and low turbidity(below 30NTU). However, PAC and PACS showed better algal removal than alum in high organic concentration($KMnO_4$ consumption above 20mg/l) and high turbidity(above 100NTU) raw water conditions generated by high algal growth, which is considered to be due to the floc settleability. In comparison of sedimentation and flotation after chemical coagulation and flocculation, the removal efficiency of organic and turbidity were higher in case of alum dose with flotation than with sedimentation, while those were better in case or PAC and PACS with sedimentation than with flotation. Thus, Alum with flotation and PAC and PACS with sedimentation is recommended for efficient algal removal. The dominant phytoplankton in raw water were Microcystic and pediastrum simplex and the removal efficiency of algae with sedimentation using alum. PAC and PACS were 27%, 45% and 22% respectively, while those with DAF showed 100% removal of phytoplankton and zooplankton.

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Changes of Monosaccharides Contents in Hydrolysates of Decomposing Plant Residues (식물성(植物性) 유기물질(有機物質)의 부숙과정중(腐熟過程中) 단당류(單糖類)의 함량(含量) 변화(變化))

  • Kim, Jeong-Je;Jang, Yong-Seon;Shin, Young-Oh
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.1
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    • pp.21-25
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    • 1990
  • The amounts of monosaccharides in acid hydrolysates of decomposing plant residues under laboratory conditions were determined. Straw of cereal rice and barley, wild grass cutting, and litters of deciduous and coniferous forest trees were treated to decompose for 90 days. Samples for the analysis of mono-saccharides were taken at 3 different periods of incubation. 1. Fractions of monosaccahrides in plant residues steadily decreased with the time of decomposition. In some samples there appeared an intermediate stages where the fractions reached the highest level. 2. Decomposition of barley straw occured at a faster rate than that of rice straw, and so did the decomposition of deciduous litter than that of coniferous litter. 3. Cereal crop residues of rice and barley were richer in monosaccharides than residues of wild grass cutting and forest litters. 4. Distiction between monosaccharides of plant origin and those of microbial origin was not possible to make in this study. 5. Glucose was the predominent monosaccharide and fucose was the monosaccharide contained in the smallest amount. No measurable ribose was detected from any sample. 6. The relative proportion of galactose in creased with the time of incubation. 7. In general, the proportion of fucose decreased with time and so did that of rhamnose, rhamnose of rice straw residue being the exception. 8. The orders of abundance of monosaccharides after decomposition of 90 days were as the following: in rice straw; glucose > xylose > arabinose > galactose > rhamnose > mannose > fucose, in barley straw; glucose > xylose > arabinose > galactose> mannose > rhamnose > fucose, in wild grass cutting; glucose > xylose > galactose arabinose> rhamnose mannose > fucose, in deciduous litter; glucose > arabinose > xylose galactose > mannose > rhamnose = fucose, and in coniferous litter; glucose > xylose > galactose > arabinose mannose > rhamnose > fucose.

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Control of Kimchi Fermentation by the Addition of Natural Antimicrobial Agents Originated from Plants (식물유래 천연항균물질 첨가에 의한 김치의 발효조절)

  • Seo, Hyun-Sun;Kim, Seonhwa;Kim, Jinsol;Han, Jaejoon;Ryu, Jee-Hoon
    • Korean Journal of Food Science and Technology
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    • v.45 no.5
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    • pp.583-589
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    • 2013
  • We investigated the delay of kimchi fermentation by the addition of plant extracts. Fifteen plant extracts were screened for inhibitory activity aginst Lactobacillus plantarum by using an agar well diffusion assay, and determined the minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) were determined. The lowest MIC for grapefruit seed extract (GFSE; 0.0313 mg/mL) was determined, followed by Caesalpinia sappan L. extract (CSLE; 0.25 mg/mL), and oregano essential oil (OREO; 1.0 mg/mL). GFSE, CSLE, and OREO were individually added to kimchi, and incubated the samples at 10 for up to 20 days. Results showed that the addition of GFSE (0.3 and 0.5%), CSLE (0.1, 0.3, and 0.5%), or OREO (0.5 and 1.0%) led to a significant increase in the pH of kimchi, and also a significant reduction in the numbers of lactic acid bacteria. Taken together, the addition of natural antimicrobial agents can delay kimchi fermentation.

Development and Validation of an Official Analytical Method for Determination of Ipfencarbazone in Agricultural Products using GC-ECD (GC-ECD를 이용한 농산물 중 Ipfencarbazone의 신규분석법 개발 및 검증)

  • Jang, Jin;Kim, Heejung;Lee, Eun-Hyang;Ko, Ah-Young;Ju, Yunji;Kim, Sooyeon;Chang, Moon-Ik;Rhee, Gyu-Seek
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.210-217
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    • 2015
  • Ipfencarbazone is a herbicide of the tetrazolinone class, and is believed to be an inhibitor of very long chain fatty acids (VLCFAs), which control cell division in weeds. The objective of this study was to develop and validate an official analytical method for ipfencarbazone determination in agricultural products. The ipfencarbazone residues in agricultural products were extracted with acetone, partitioned with n-hexane, and then purified through silica SPE cartridge. Finally, the analyte was quantified by gas chromatograph-electron capture detector (GC-ECD) and confirmed with gas chromatograph/mass spectrometer(GC/MS). The linear range of ipfencarbazone was 0.01 to 1.0 mg/L with the coefficient of determination ($r^2$) of 0.9999. The limit of detection (LOD) and quantification (LOQ) was 0.003 and 0.01 mg/kg, respectively. In addition, average recoveries of ipfencarbazone ranged from 80.6% to 112.3% at the different concentration levels LOQ, 10LOQ and 50LOQ, while the relative standard deviation was 2.2-8.6%. All values were consistent with the criteria ranges requested in the CODEX guidelines. Furthermore, and inter-laboratory study was conducted to validate the method. This proposed method for determination of ipfencarbazone residues in agricultural products can be used as an official analytical method.

Geochemical Characteristics on Geological Groups of Stream Sediment in the Boseong-Hwasun Area, Korea (보성-화순지역 하상퇴적물에 대한 지질집단별 지구화학적 특성)

  • Park, Young-Seog;Kim, Jong-Kyun
    • Journal of the Korean earth science society
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    • v.32 no.7
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    • pp.707-718
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    • 2011
  • We study the natural background and geochemical characteristics on geological groups of stream sediment in the Boseong-Hwasun area. We collected 186ea stream sediment samples along the primary channels and dried them naturally in laboratory. The contents of major, trace and rare earth elements were determined by XRF, ICP-AES and NAA analysis methods. In order to know the natural background and geochemical characteristics of geological groups, we classified the studied area into granitic gneiss (GGn) area and porphyroblastic gneiss (PGn) area. The contents range of major elements for GGn area is $SiO_2$ 45.5-73.09 wt.%, $Al_2O_3$ 12-20.76 wt.%, $Fe_2O_3$(T) 3.72-8.85 wt.%, $K_2O$ 2.38-4.2 wt.%, MgO 0.75-2.77 wt.%, $Na_2O$ 0.78-1.88 wt.%, CaO 0.27-2.1 wt.%, $TiO_2$ 0.56-1.72 wt.%, $P_2O_5$ 0.06-0.73 wt.% and MnO 0.03-0.95 wt.%, and for PGn area it is $SiO_2$ 43.74-70.71 wt.%, $Al_2O_3$ 11.54-25.05 wt.%, $Fe_2O_3$(T) 3.44-13.46 wt.%, $K_2O$ 2.08-3.86 wt.%, MgO 0.65-2.99 wt.%, $Na_2O$ 0.63-1.7 wt.%, CaO 0.35-2.07 wt.%, $TiO_2$ 0.68-4.17 wt.%, $P_2O_5$ 0.1-0.31 wt.% and MnO 0.07-0.33 wt.%. The contents range of hazard elements for GGn area is Cr 41.7-242 ppm, Co 7.6-25.1 ppm, Ni 12-61 ppm, Cu 10-47 ppm, Zn 48.5-412 ppm, Pb 17-215 ppm, and for PGn area, it is Cr 29.6-454 ppm, Co 5.9-53.7 ppm, Ni 8.7-287 ppm, Cu 6.4-134 ppm, Zn 43.6-370 ppm, Pb 15-37 ppm area. There is a good correlation between Cr and MgO and Co among $Al_2O_3$, $Fe_2O_3$(T), MgO and Ni among $Fe_2O_3$(T), CaO, MgO whereas Cu, Zn and Pb have a low correlation for major elements in GGn area. Generally Cr, Co, Ni, and Cu have a good correlation with major elements, but a low correlation with Zn and Pb in PGn area.

An Outbreak of Mumps in Taejon. Korea. 1998 (1998년 대전지역에서 유행한 볼거리의 임상적 연구)

  • Kang, Hee-Dong;Lee, Kyung-Yil;Cha, Sang-Won;Yoon, Kye-Nam;Lee, Dong-Joan;Han, Ji-Whan;Kang, Jin-Han;Whang, Kyung-Tai
    • Pediatric Infection and Vaccine
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    • v.6 no.2
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    • pp.239-244
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    • 1999
  • Purpose : In 1998, there was an outbreak of mumps in Taejon, Korea. We analyzed the monthly incidence, clinical manifestations, and complications during the outbreak and the annual incidence of the recent ten years. Methods : We reviewed the clinical records of 35 mumps patients who had been admitted to Taejon St. Mary's Hospital from January to December, 1998. Results : Mumps was prevalent in summer(from June to August) with 20 cases(57%). Thirthy-three patients(94.3%) received MMR at 15 months of age and no patients received a booster mumps vaccine. The mean age of the mumps patients was 10.5 years-old and the majority of mumps(71%) occurred between 5 and 12 year of age. The male female ratio was 2.2 : 1. The clinical manifestations were swelling of parotid gland(100%), fever (91%), vomiting(77%), abdominal pain(74%) and headache(66%) in order of frequency. Meningitis occurred in 20 cases(57%) and the mean WBC count of CSF was $464{\pm}263/mm^3$. In serology, the mean serum amylase level was $483{\pm}294IU/dL$ and anti-mumps IgM and IgG were positive in 11 and 15 out of 18 cases, respectively. From 1989 to 1997, the mean number of inpatients with mumps was 3.4 per year. Conclusion : We experienced an outbreak of mumps in 1998. It is suggested that there was a primary mumps vaccine failure in Korea and that further studies for vaccine faiure and method of booster mumps vaccine are necessary.

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Resistance and Susceptibility of Diamondback Moth, Plutella xylostella Strains Collected from Different Region in Korea to Bacillus thuringiensis (국내 지역별 채집계통 및 감수성계통 배추좀나방에 대한 Bacillus thuringiensis 제품의 생물활성 비교)

  • Kim, Young-Rim;Cho, Min-Su;Oh, Se-Mun;Kim, Sung-Woo;Youn, Young-Nam;Yu, Yong-Man
    • The Korean Journal of Pesticide Science
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    • v.14 no.2
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    • pp.123-132
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    • 2010
  • Six populations of the diamondback moth, Plutella xylostella, were collected from the different national areas for resistance and reared in laboratory for two sensitive population. These populations of P. xylostella were examined the developed resistance against commercial products of Bacillus thuringiensis. There were 3 products with B. thuringiensis subsp. kurstaki including Tyuneup$^{(R)}$, Thuricide$^{(R)}$ and Geumulmang$^{(R)}$ and 2 products with B. thuringiensis subsp. aizawai including Tobagi$^{(R)}$ and Scorpion$^{(R)}$. The sensitive population of diamondback moths were provided from National Academy of Agricultural Science (NP) and Highland Agriculture Research Center (GR population) and field populations were caught from 6 different national areas. Resistance against Tyuneup$^{(R)}$ was developed 4.8 and 2.5 times in SP and HS compared with GR population of diamondback moth, respectively. In case of Geumulmang$^{(R)}$, it was developed 9.9 and 6.8 times in SP and NM population compared with NP population, respectively. Otherwise, Tobagi$^{(R)}$ was showed higher resistance in HS than any other population compared with GR population, however, Scorpion$^{(R)}$ that is a same strain with Tobagi$^{(R)}$, was showed only double resistance to SP population. It was supposed that the development of resistance to B. thuringiensis might be caused by the continuous application of the specific commercial product at the specific area. So, we need to use the commercial products of B. thuringiensis in rotation with different B. thuringiensis strains. In the other hand, when HS population with highest resistance were reared in laboratory, their resistance ratio was rapidly dropped to 1.1 times at second generation. We have to examined the resistance mechanism of the diamondback moth to B. thuringiensis strains.

Development and growth in fertilized eggs and larvae of Korea swift's scallop Chlamys swiftii reared in the laboratory (실험실에서 사육된 고랑가리비 Chlamys swiftii 수정란 발생과 유생 성장)

  • Lee, Chu;Kim, Yi Cheong;Kim, Gi Seung;Nam, Myung-Mo
    • The Korean Journal of Malacology
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    • v.29 no.4
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    • pp.263-272
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    • 2013
  • The development of swift's scallop Chlamys swiftii, reared in the laboratory, has been examined through the investigation of morphological characteristics in fertilized egg, larvae and juvenile. Eggs were fertilized with a dilute sperm solution to improve the survival of fertilized eggs. Developing larvae were maintained at a temperature of $16{\pm}0.5^{\circ}C$ and salinity concentration of 33 ppt. We have investigated the fertilization rates and egg number spawned at several stimulating conditions such as sunlight exposure, air dry, seawater temperature rise ($5^{\circ}C$) and seawater temperature rise ($5^{\circ}C$) after exposure of air dry for spawning induction of swift's scallop Chamys swiftii. Stimulation treated with sunlight exposure and seawater temperature rise ($5^{\circ}C$) have shown the spawning number of 700,000-900,000 and 700,000-800,000 per individual, respectively while stimulation treated with seawater temperature rise ($5^{\circ}C$) after exposure of air dry have shown the high spawning number of 1,000,000-1,500,000 per individual. Survival rate of D-shaped larvae of swift's scallop put into the different seawater temperatures of $8^{\circ}C$, $12^{\circ}C$, $16^{\circ}C$, $20^{\circ}C$ and $24^{\circ}C$ has been 4.1%, 11.6%, 32.7%, 18.6% and 3.2%, respectively. Fertilized eggs with the diameter of $72{\mu}m$ developed into trochophore larvae of $103{\pm}3.8{\mu}m$ shortly after 35 hours and to D larvae of $129{\pm}10.4{\mu}m$ shell length within 72 hours. It took 336 hours to become initial Umbo-stage larva of $145{\pm}16.8{\mu}m$ shell length. Post larvae, which have been $197{\pm}13.6{\mu}m$ shell length, spontaneously have settled in the attachment substances. It have required 528 hours from fertilized eggs to early attached juvenile to become initial juvenile size of $245{\pm}15.8{\mu}m$ shell length.

Heat Shield Property of Nanostructural-regulated Fe2O3/TiO2 Composites Filled with Polyacrylate Paint (나노구조 변화에 의한 Fe2O3/TiO2 복합재료를 충전한 Poly Acrylate 도료의 열차단 특성)

  • Kim, Dae Won;Ma, Young Kil;Kim, Jong Seok
    • Applied Chemistry for Engineering
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    • v.31 no.1
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    • pp.43-48
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    • 2020
  • Fe2O3 nanoparticles with the mixed structure of cubic and nanorod were synthesized by precipitation, hydrothermal, sol-gel method, etching process and heat treatment. Fe2O3/TiO2 core-shell (CS) of type Fe2O3@TiO2 composite was fabricated on a 20 nm nanolayer of TiO2 coated on the surface of Fe2O3 nanoparticles. Fe2O3/TiO2 yolk-shell (YS) composite was prepared by chemical etching and heat treatment of Fe2O3/TiO2 CS nanoparticles. Physical properties of Fe2O3, Fe2O3@TiO2 CS and Fe2O3@TiO2 YS nanoparticles were characterized by FE-SEM, HR-TEM and X-ray diffraction. The solar reflectance, commission internationale de l'Elcairage (CIE) color coordinate and heat shield temperatures of Fe2O3, CS and YS type Fe2O3@TiO2 pigments filled with poly acrylate (PA) paints were investigated by UV-Vis-NIR spectrometer and homemade heat shield temperature measuring device. The Fe2O3@TiO2 YS red pigment filled PA composite exhibited excellent near infrared light reflecting performance and also reduced the heat shield temperature of 13 ℃ than that of Fe2O3 filled counterparts.