The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Resistance and Susceptibility of Diamondback Moth, Plutella xylostella Strains Collected from Different Region in Korea to Bacillus thuringiensis

국내 지역별 채집계통 및 감수성계통 배추좀나방에 대한 Bacillus thuringiensis 제품의 생물활성 비교

  • Kim, Young-Rim (Dept. of Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Cho, Min-Su (Dept. of Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Oh, Se-Mun (Dept. of Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Kim, Sung-Woo (Dept. of Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Youn, Young-Nam (Pesticide Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Adminstration) ;
  • Yu, Yong-Man (Pesticide Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Adminstration)
  • 김영림 (농촌진흥청 국립농업과학원 농자재평가과) ;
  • 조민수 (농촌진흥청 국립농업과학원 농자재평가과) ;
  • 오세문 (농촌진흥청 국립농업과학원 농자재평가과) ;
  • 김성우 (농촌진흥청 국립농업과학원 농자재평가과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물학과)
  • Received : 2010.03.29
  • Accepted : 2010.04.12
  • Published : 2010.06.30
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Abstract

Six populations of the diamondback moth, Plutella xylostella, were collected from the different national areas for resistance and reared in laboratory for two sensitive population. These populations of P. xylostella were examined the developed resistance against commercial products of Bacillus thuringiensis. There were 3 products with B. thuringiensis subsp. kurstaki including Tyuneup$^{(R)}$, Thuricide$^{(R)}$ and Geumulmang$^{(R)}$ and 2 products with B. thuringiensis subsp. aizawai including Tobagi$^{(R)}$ and Scorpion$^{(R)}$. The sensitive population of diamondback moths were provided from National Academy of Agricultural Science (NP) and Highland Agriculture Research Center (GR population) and field populations were caught from 6 different national areas. Resistance against Tyuneup$^{(R)}$ was developed 4.8 and 2.5 times in SP and HS compared with GR population of diamondback moth, respectively. In case of Geumulmang$^{(R)}$, it was developed 9.9 and 6.8 times in SP and NM population compared with NP population, respectively. Otherwise, Tobagi$^{(R)}$ was showed higher resistance in HS than any other population compared with GR population, however, Scorpion$^{(R)}$ that is a same strain with Tobagi$^{(R)}$, was showed only double resistance to SP population. It was supposed that the development of resistance to B. thuringiensis might be caused by the continuous application of the specific commercial product at the specific area. So, we need to use the commercial products of B. thuringiensis in rotation with different B. thuringiensis strains. In the other hand, when HS population with highest resistance were reared in laboratory, their resistance ratio was rapidly dropped to 1.1 times at second generation. We have to examined the resistance mechanism of the diamondback moth to B. thuringiensis strains.

국내 난방제 해충의 하나인 배추좀나방(Plutella xylostella)을 방제하기 위하여 사용되어온 상업용 Bacillus thuringiensis 제품들을 이용하여 국내 6개 지역에서 채집한 지역계통과 2개의 감수성계통 배추좀나방에 대한 저항성 발달정도를 검토하였다. 상품화된 B. thuringiensis subsp. kurstaki 계통인 Tyuneup$^{(R)}$, Thuricide$^{(R)}$, Geumulmang$^{(R)}$ 등 3제품과 B. thuringiensis subsp. aizawai 계통인 Tobagi$^{(R)}$, Scorpion$^{(R)}$ 등 2제품을 이용하였다. 공시충으로 사용된 배추좀나방은 계대사육중인 감수성개체군 NP와 GR 2종류이며, 전국의 배추밭에서 채집한 SP, HS, NM, DR, HC, HW개체군 등 6종류를 야외 개체군으로 사용하였다. Tyuneup$^{(R)}$ 제품의 생물검정에서 감수성 계통인 고령지시험장계통의 GR과 비교해 SP 개체군은 4.8배를 그리고 HS 개체군은 2.5배의 저항성발달을 보였다. Geumulmang$^{(R)}$ 제품의 경우에는 감수성계통인 NP 개체군에 비하여 SP 개체군에서 9.9배와 NM 개체군에서 6.8배정도의 저항성을 나타냈다. Tobagi$^{(R)}$는 HS 개체군에서 GR 개체군에 비해 14배의 저항성을 보여 가장 높게 나타났다. 그러나 동일 계통의 Scorpion$^{(R)}$은 SP 개체군에 약 2배정도의 저항성을 보여주고 있을 뿐이다. 이러한 결과는 어떤 특정한 B. thuringiensis 제품이 특정지역에 집중적으로 계속 사용함으로서 저항성이 발달되는 것으로 판단되며 종류가 다른 B. thuringiensis 제품을 교호 살포해야 할 것으로 사료된다. 그러나 Tobagi$^{(R)}$에 14배의 저항성 발달을 보인 HS 개체군을 실험실에서 동일약제에 연속으로 노출시켰을 때 $F_2$세대에서는 급격히 저항성 수치가 떨어져 1.1로 나타나 저항성 기작의 검토가 필요하다.

Keywords

References

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