• Title/Summary/Keyword: 실험군

Search Result 7,466, Processing Time 0.04 seconds

Protective Effects of Korean Panax Ginseng Extracts against TCDD-induced Toxicities in Rat (랫드에서 TCDD 투여에 의해 유도된 생체독성의 고려홍삼 추출물에 의한 억제 효과)

  • Choi, Soo-Jin;Sohn, Hyung-Ok;Shin, Han-Jae;Hyun, Hak-Cheol;Lee, Dong-Wook;Song, Yong-Bum;Lee, Soo-Hyun;Gang, Dong-Ho;Lim, Hak-Seob;Lee, Cheol-Won;Moon, Ja-Young
    • Journal of Ginseng Research
    • /
    • v.32 no.4
    • /
    • pp.382-389
    • /
    • 2008
  • To achieve a better understanding of protective effects of water extracts of Panax ginseng against TCDD-induced toxicities, we monitored physiological and clinical changes in rat for 4 weeks after administrations of each Panax Ginseng extract or TCDD, and co-administration of the two materials. For this study, 120 male Sprague-Dawley (SD) rats weighing 190-210 g each (8 weeks old) were divided into four groups: TCDD-administered, co-administered group with TCDD and ginseng extract, ginseng extract-administered, and control group. The TCDD-administered group received single dose of TCDD in a corn oil vehicle ($25\;{\mu}g/kg$ body weight) by intraperitoneal administration on Day 1. The Panax ginseng extracts-administered group received intraperitoneally 100 mg/kg body weight every other day for one month. For the co-administered group with TCDD and ginseng extracts, Panax ginseng extracts were intraperitoneally administered to rats at 100 mg/kg body weight every other day for one month after a single intraperitoneal dose of $25\;{\mu}g$ of TCDD/kg body weight on Day 1. Panax ginseng extracts attenuated the mortality induced by TCDD administration. The extracts also slightly attenuated the TCDD-induced body weight loss. Administration of TCDD alone increased liver weight at 2, 5, and 16 days after administration of TCDD. Administration of Panax ginseng extracts rather decreased liver weight through whole the experimental period, but which was statistically insignificant. Administration of TCDD alone at $25\;{\mu}g/kg$ body weight increased both serum enzyme activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) at 32 days, indicating that liver damage occurred maximally at that time. Ginseng extract administration caused insignificant changes in serum ALT, but gradually decreased in AST as the exposure time increased. Coadministration of TCDD and ginseng extracts caused serum AST activity to significant recovery to normal value at 16 days and 32 days after exposure to TCDD. The extracts also significantly decreased the TCDD-induced ALT activity after 16 days of TCDD administration. These results suggest that Panax ginseng extracts may possess a protective effect against TCDD-induced toxicities including hepatotoxicity in rats.

The Effects of Aromatherapy on Elders' Chronic Pain, Sleep (아로마테라피가 노인의 만성통증과 수면에 미치는 효과)

  • Lee, Hye-Kyung
    • Journal of Digital Convergence
    • /
    • v.14 no.7
    • /
    • pp.385-395
    • /
    • 2016
  • This research applied Aromatherapy Foot Massage on Community elders and examined its effects on their chronic pain, sleep. The study subjects were 43 female elders in the community of including 22people for the experimental group and 21 for the control group. The aromatherapy was carried out 6times for 2weeks to the experimental group, 3times a week and 10minutes per session. The components of aroma essential oil included Chamomile roman, Lavender, Marjoram sweet, and Orange sweet that were diluted 20% into 100mL of Jojoba oil which was the carrier oil. The results, the experimental group that participated in the aromatherapy improved in chronic pain, quality of sleep, and the number of urination during sleep decreased compared to the control group. The research results demonstrated that due to the aroma foot massage, chronic pain decreased, sleep improved.

The expression of MMP-1, -8, and -13 mRNA in the periodontal ligament of rats during tooth movement with cortical punching (백서의 치아이동 시 피질골 천공이 치주조직의 MMP-1, -8, -13 mRNA의 발현에 미치는 영향)

  • Gwack, Choon;Kim, Seong-Sik;Park, Soo-Byung;Son, Woo-Sung;Kim, Yong-Deok;Jun, Eun-Sook;Park, Mi-Hwa
    • The korean journal of orthodontics
    • /
    • v.38 no.3
    • /
    • pp.187-201
    • /
    • 2008
  • Objective: The aim of this study was to determine whether cortical punching stimulates the expression of matrix metalloproteinase-1, -8, and -13 in orthodontic tooth movement in rats. Methods: A total of 32 male sprague-dawley rats at 15 weeks old were divided into two groups of 16 rats each, to form the tooth movement with cortical punching (TMC) group and tooth movement only (TM) group. A total of 20 gm of orthodontic force was applied to rat incisors to cause experimental tooth movement. Cortical punching was performed on the palatal side near the central incisor with a 1.0 mm width microscrew in the TMC group. The duration of tooth movement was 1, 4, 7, and 14 days. Results: Measurements of the mRNA expression were selected as the means to determine the identification of expression of MMP-1, -8, and -13. In the TMC group, the expression of collagen type I was greater than that of the TM group from day 4 to day 14. Expression of TIMP-1 in the TM group was greater than that of the TMC group in the pressure side of PDL and alveolar bone cell at day 4. In the TMC group, TIMP-1 was expressed at the osteoclast, but not at the tooth surface of the TM group at day 14, Maximum induction of the mRNA of MMP-1 was observed on day 4 in the TMC group, but it was observed on day 7 in the TM group. MMP-8 mRNA of the TMC group was twice greater than that of the TM group at f days. In the TMC group, maximum induction of MMP-13 mRNA was observed on day 1. Conclusions: These findings suggested that cortical punching can stimulate remodeling of PDL and alveolar bone connective tissues during experimental orthodontic tooth movement in rats.

A gene filtering method based on fuzzy pattern matching for whole genome microarray data analysis (마이크로어레이 데이터의 게놈수준 분석을 위한 퍼지 패턴 매칭에 의한 유전자 필터링 방법)

  • Lee, Seon-A;Lee, Geon-Myeong;Lee, Seung-Ju;Kim, Won-Jae;Kim, Yong-Jun;Bae, Seok-Cheol
    • Proceedings of the Korean Institute of Intelligent Systems Conference
    • /
    • 2007.11a
    • /
    • pp.145-148
    • /
    • 2007
  • 생명과학분야에서 마이크로어레이 기술은 세포에서의 RNA 발현 프로파일을 관찰할 수 있도록 함으로써 생명현상의 규명 및 약물개발 둥에서 분자수준의 생명현상에 대한 관찰과 분석이 가능 해지고 있다. 마이크로어레이 데이터분석에서는 특정한 처리나 과정에서 현저한 특성을 보이는 유전자를 식별하기 위한 분석뿐만 아니라 유전자 전체인 게놈수준에서의 분석도 이루어진다. 최근 유전자의 발현이 다양한 조절, 신호전달 및 대사경로에 의해서 영향을 받고 있다는 관점에서 게놈수준의 분석에 관심이 증가하고 있다. 약물반응 실험에서는 약물에 대한 게놈수준의 발현 프로파일을 관찰하는 것도 많은 정보를 제공할 수 있다. 약물실험에서는 대조군과 실험군들간에 관심 있는 상대적인 발현특성을 갖는 유전자군을 전체적으로 추출하는 것이 필요한 경우가 있다. 예를 들면 정상군은 두개의 실험군에 대해서 중간청도의 발현정도를 갖는 유전자군을 식별하는 분석을 하는 경우, 생물학적인 데이터의 특성상 절대값을 비교하는 방법으로는 유용한 유전자들을 효과적으로 식별해 낼 수 없다. 이 논문에서는 정상군과 실험군들의 발현정도값의 경향을 판단하기 위해서 각 유전자에 대해서 집단별 대표값을 선정하여 퍼지집합으로 집단의 값의 범위를 결정하고, 이를 이용하여 특정 패턴을 갖는 유전자들을 식별해내는 방법을 제안하고, 실제 데이터를 통해서 실험한 결과를 보인다.

  • PDF

Effect on Oral Health and Child Oral Health-related Quality of Life through School-based Toothbrushing Program in School after 42 Months (42개월 경과시점의 양치교실사업이 구강건강과 아동 구강건강관련 삶의 질에 미치는 효과)

  • Cho, Min-Jeong;Koong, Hwa-Soo;Hwang, Soo-Jeong;Song, Eun-Joo;Choi, Yu-Kyoung
    • Journal of dental hygiene science
    • /
    • v.12 no.2
    • /
    • pp.139-144
    • /
    • 2012
  • Daejeon school-based toothbrushing program using toothbrushing room started at 2007. Ministry of Health and Welfare started national toothbrushing room program without pilot project at 2011. The aim of this study was to provide the basic data to assess of the effect of this program. 59 experimental subjects and 60 control subject were selected after written consent. Oral Examination was done by 1 dentist using WHO standard and Survey of Child Oral Health-Related Quality of Life(COHIP), oral health knowledge and oral health behavior were done using self-registration form. We analyzed the result after adjusting economic status and subjective for the difference between experimental and control groups using SPSS 19.0. The experimental group had lower plaque index, better COHIP and more oral health knowledge significantly(p<0.05). But, The significance was not found in dental caries index and oral health behavior. We suggested the oral health education program and other dental caries prevention program including sealants were needed to prevent dental caries, although school-based toothbrushing program could improve COHIP.

THE EFFECTS OF NANO-SIZED HYDROXYAPATITE ON DEMINERALIZATION RESISTANCE AND BONDING STRENGTH IN LIGHT-CURED GLASS IONOMER DENTAL CEMENT (광중합형 글라스아이오노머 시멘트의 탈회 저항성과 결합 강도에 대한 나노미터 입자의 하이드록시아파타이트의 효과)

  • Kim, Ji-Hee;Lee, Yong-Keun;Kim, Seong-Oh;Song, Je-Seon;Choi, Byung-Jai;Choi, Hyung-Jun
    • Journal of the korean academy of Pediatric Dentistry
    • /
    • v.37 no.1
    • /
    • pp.24-34
    • /
    • 2010
  • The aim of this study was to evaluate the effect of incorporated nano HA on the demineralization resistance and bonding strength of LC GIC in comparison with micro HA. Fuji II LC GIC was used as the control group and a base material for experimental groups. Two experimental groups were prepared. One was prepared by adding 15% micro HA to LC GIC by weight ratio (Exp. 1), and the other was prepared by adding 15% nano HA instead (Exp. 2). According to the results, the following conclusions could be obtained. 1. Observing under the CLSM, the control group showed thicker enamel demineralization layer than in the experimental groups, and the Exp. 2 group showed the thinnest demineralization layer. 2. In SEM analysis, there was greater enamel demineralization in the control group. The Exp. 2 group was more resistant to demineralization compared to the Exp. 1 group. 3. The bonding strength was found to be in the increasing order of control, Exp. 1, and Exp. 2 group (p < 0.05). 4. Observing the fractured surfaces under SEM after the bonding strength test was performed, there were bone-like apatite particles formed in HA-added experimental groups, and a greater number of bone-like apatite particles were formed in the Exp. 2 group compared to the Exp. 1 group.

Comparative Study on Effects of Sexuality Education in Elementary School Students (초등학생의 성교육 효과에 관한 비교연구)

  • Seo, Soon-Hee;Hong, Jee-Young;Lee, Moo-Sik;Na, Baeg-Ju;Lee, Jin-Yong;Bae, Seok-Hwan;Yoo, Se-Jong
    • Proceedings of the KAIS Fall Conference
    • /
    • 2010.11b
    • /
    • pp.513-516
    • /
    • 2010
  • 본 연구는 성교육이 학생들의 성 지식, 태도, 역할 정체감에 미치는 효과를 알아보기 위해 실험군과 대조군으로 집단을 나누어 실험군에게 의도적인 성교육 실시하여 그 결과를 살펴보고 향후 학교에서 실시되는 성교육 계획 수립에 도움을 주고자 하는 실험연구이다. 본 연구의 대상은 충청북도 제천시에 위치한 J초등학교 5학년 6개 학급을 대상으로 실험군 100(남 50, 여 50), 대조군 100(남 50, 여 50) 총 200명을 대상으로 실시하였으며 자료 수집은 2009년 10월 15일부터 2009년 11월 25일까지 실시하였다. 연구도구는 문헌고찰을 통해 성 지식, 성 태도, 성역할 정체감에 사용한 설문지를 구성타당도 검증을 통해 문항을 구성 선정하였으며, 신뢰도는 Cronbach's ${\alpha}$ 값으로 검증하였다. 연구 대상자의 일반적 특성을 보면 92%의 학생이 부모님이 있고, 88.5%가 형제자매가 있으며, 집안 분위기는 60%가 자유스럽고, 7%가 엄한 것으로 나타났다. 연구 대상자의 성관련 특성으로 가정에서 성교육을 받아본 경험이 있는 경우는 36%로 나타났으며, 성과 관련된 정보를 얻는 순서는 87.5%의 학생이 학교 보건선생님한테 성 관련 정보를 얻는 경우가 대부분이었고, 다음 순서로는 부모님, 서적류, 담임선생님, 영상매체, 친구, 형제자매 순으로 나타났다. 연구 대상자의 신체변화정도를 살펴보면 여자의 경우 월경이 있는 경우는 20%정도, 가슴의 변화는 84%로 나타났으며, 남자의 경우 몽정이 있는 경우는 4%로 나타났으며, 남녀 모두 변성기, 여드름, 발모, 몽정, 월경, 가슴변화 등 한 가지 이상 신체변화가 나타난 경우는 56%로 나타났다. 연구 대상자의 성지식 사전 사후 점수를 비교해보면 두 집단 모두 사전보다 사후 점수가 증가하였다. 사후검사에서 교육을 받지 않은 대조군의 성지식 점수는 소폭 증가하였으나 성교육을 실시한 실험군의 성지식 점수는 더 큰 폭으로 높았으며, 특히 신체발달에서 사전검사에 대조군은(M=2.17), 실험군은 (M=1.66)으로 실험군의 점수가 낮았으나, 사후검사에서는 대조군은(M=2.76), 실험군은(M=3.90)으로 실험군의 점수가 더 높았다. 이상의 결과를 종합해보면 학교에서의 체계화된 성교육은 학생들의 성 지식, 태도, 성 역할 정체감 형성에 도움을 줄뿐만 아니라 성교육을 부끄러워하고, 쑥스러워하며, 어색해 하던 아이들이 문제 중심 학습을 적용하여 자기 주도적으로 문제를 해결하고, 체험, 실습, 역할극, 토의 등의 활동적인 수업에 적극 참여하고, 자연스럽게 활동하여 긍정적인 '밝은 성(性)'으로 변해가는 모습을 관찰할 수 있었다. 성교육은 인간의 성장과 발달에 따라 변화하는 과정이라는 것을 인식하여 건전한 성, 건강한 성을 함양하기 위해 체계적이고 발달 단계에 맞추어 이루어져야 할 것이다.

  • PDF

The Effects of Team-Based Learning(TBL) for Edutainment Activation in Nursing Students (간호대학생의 에듀테인먼트 활성화를 위한 팀기반학습(TBL) 효과)

  • Kim, Yu-Jeong
    • Journal of Korea Entertainment Industry Association
    • /
    • v.13 no.7
    • /
    • pp.397-408
    • /
    • 2019
  • Purpose: The purpose of this study was to examine the effects of team-based learning(TBL) program on problem solving ability, self-directed learning and learning motivation of nursing students in a adult nursing course. Methods: This study was quasi-experimental study by using nonequivalent control group pretest-posttest design. Participants were 132 nursing students consisting of each experiment group 66 and control group 66. The experiment group was provided TBL program for 2 hours per session during 6 sessions and control group was provided lecture. Data were collected by questionnaires from April 20 to June 19, 2018. Data was analyzed by a chi-square test, t-test and ANCOVA using IBM SPSS 21.0 program. Results: The mean scores of problem solving ability(F=9.179, p=.003), self-directed learning(F=7.447, p=.007) and learning motivation(F=7.974, p=.006) were significantly higher in experiment group than those in control group. Conclusion: TBL program in a adult nursing course was effective in problem solving ability, self-directed learning and learning motivation of nursing students.

쌀의 tocol 획분이 고콜레스테롤 식이를 섭취한 흰쥐의 체내 지질함량에 미치는 영향

  • 한송이;하태열;김성란;김인환;이현유
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
    • /
    • 2003.10a
    • /
    • pp.225.2-226
    • /
    • 2003
  • 본 연구에서는 현미로부터 tocoles 획분을 분리하여 고콜레스테롤 식이를 섭취한 흰쥐의 체내 지질대사에 미치는 영향을 조사하였다. 실험동물은 SD계 숫컷 5주령을 구입하여 정상군, 대조군 (1% cholesterol), rice oil 첨가군, tocoles 첨가군의 4군으로 나누어 AIN-76 diet 조성에 의거하여 식이를 조제하여 4주 동안 섭취시켰다. 실험기간이 끝난 후 복부 대동맥으로부터 채혈하고 각 조직을 적출하여 간, 신장, 비장 및 부고환지방 조직의 무게를 측정하였다. 또한 혈청 lipid profile과 TBARS 함량 및 ALT, AST 및 ALP 활성을 분석하였고 간장중 중성지방, 콜레스테롤 및 TBARS 함량과 G6PDH 및 malic enzyme을 분석하였다. 그 결과 간장 및 부고환지방의 무게가 대조군에서 유의적으로 증가하였으나, tocoles 첨가군은 정상군의 수준으로 감소하였다. 혈청 내의 중성지방과 총 콜레스테롤은 실험군 간에 유의적인 차이는 나타내지 않았으나 감소하는 경향을 보였다. 간장 조직 내 지질함량은 대조군에서 높게 나타났으며, tocoles 군에서 중성지방과 콜레스테롤 및 총 지방의 함량이 유의하게 감소하였다. 지질 과산화물 함량은 혈청과 간장 조직 모두 유의적으로 감소하였다.

  • PDF

Antioxidative and Antiaging Effects of Dietary Yellow and Black Soybean in Rats (노란콩 및 검정콩의 섭취가 흰쥐의 항산화 및 항노화 시스템에 미치는 영향)

  • 류승희;문갑순
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.32 no.4
    • /
    • pp.591-597
    • /
    • 2003
  • To investigate antioxidative and antiaging effects of yellow and black soybeans in vivo system, male SD rats (n=24) were fed the diets containing casein (C), yellow soybean (YS) or black soybean (BS) for 8 weeks. Experimental groups showed the preventive effect on lipid and protein oxidation, especially protein oxidation of plasma was significantly inhibited in BS group. SOD and catalase activities were not different among the groups, but hepatic glutathione peroxidase (GSH-px) activity was significantly lowered in YS and BS groups compared with control group. The contents of superoxide anion radical in cytosol were significantly lowered in experimental groups compared with control group. And hydroxyl radical was slightly lowered in soybean groups. Lipofuscin accumulation on the heart and eyes of rats was effectively inhibited by yellow soybean and black soybean, respectively. In conclusion, these results suggest that yellow soybean and black soybean have the antioxidative/antiaging effect in vivo system and they have similar activities despite different color of seed coat.