• Journal of Marine Life Science
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• v.9 no.1
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• pp.9-21
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• 2024

Paralytic shellfish poisoning (PSP) including Saxitoxin (STX) is caused by harmful algae, and poisoning occurs when the contaminated seafood is consumed. The mouse bioassay (MBA), a standard test method for detecting PSP, is being sanctioned in many countries due to its low detection limit and the animal concerns. An alternative to the MBA is the Neuro-2a cell-based assay. This study aimed to establish various test conditions for Neuro-2a assay, including cell density, culture conditions, and STX treatment conditions, to suit the domestic laboratory environment. As a result, the initial cell density was set to 40,000 cells/well and the incubation time to 24 hours. Additionally, the concentration of Ouabain and Veratridine (O/V) was set to 500/50 μM, at which most cells died. In this study, we identified eight concentrations of STX, ranging from 368 to 47,056 fg/μl, which produced an S-shaped dose-response curve when treated with O/V. Through inter-laboratory variability comparison of the Neuro-2a assay, we established five Quality Control Criteria to verify the appropriateness of the experiments and six Data Criteria (Top and Bottom OD, EC₅₀, EC₂₀, Hill slop, and R² of graph) to determine the reliability of the experimental data. The Neuro-2a assay conducted under the established conditions showed an EC₅₀ value of approximately 1,800~3,500 fg/μl. The intra- & inter-lab variability comparison results showed that the coefficients of variation (CVs) for the Quality Control and Data values ranged from 1.98% to 29.15%, confirming the reproducibility of the experiments. This study presented Quality Control Criteria and Data Criteria to assess the appropriateness of the experiments and confirmed the excellent repeatability and reproducibility of the Neuro-2a assay. To apply the Neuro-2a assay as an alternative method for detecting PSP in domestic seafood, it is essential to establish a toxin extraction method from seafood and toxin quantification methods, and perform correlation analysis with MBA and instrumental analysis methods.

• Journal of the korean academy of Pediatric Dentistry
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• v.25 no.4
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• pp.683-690
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• 1998

The purpose of this study was to evaluate the reliablity of laser Doppler flowmeter on the permanent maxillary central incisors with open apex. Laser Doppler flowmeter and electric pulp tests were done in 35 elementary students aged between 8 and 9 years and 35 females and males aged between 23 and 24 years. Teeth with open apex were confined to root developmental stage 5 and 6 by Moorrees classification. The threshold of electric pulp tests was decided the time of tingling sensation. More than three different electric pulp tests applied on every teeth with more than 3 minutes interval between each teeth due to the false response. Laser Doppler flowmeter test stablilzation and observation stages took more than 5 minutes each LDF values and electric pulp test thresholds were analyzed with student t-test. The results were as follows: 1. The electric pulp test threshold on teeth with immature and mature roots showed 28.4% and 100% response respectively, and the the LDF values on teeth with immature and mature roots showed 100% response. 2. The EPT thresholds on teeth with immature roots were larger than thresholds on teeth with mature roots(p<0.01). 3. No significant differences were found in the LDF values on teeth with immature and mature roots.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.8
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• pp.721-728
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• 2017

High-grade gray cast iron (HCI350) was prepared by adding Cr, Mo and Cu to the gray cast iron (GC300). Their microstructure, mechanical properties and fatigue strength were studied. Cast iron was made from round bar and plate-type castings, and was cut and polished to measure the percentage of each microstructure. The size of flake graphite decreased due to additives, while the structure of high density pearlite increased in volume percentage improving the tensile strength and fatigue strength. Based on the fatigue life data obtained from the fatigue test results, the probability - stress - life (P-S-N) curve was calculated using the 2-parameter Weibull distribution to which the maximum likelihood method was applied. The P-S-N curve showed that the fatigue strength of HCI350 was significantly improved and the dispersion of life data was lower than that of GC300. However, the fatigue life according to fatigue stress alleviation increased further. Data for reliability life design was presented by quantitatively showing the allowable stress value for the required life cycle number using the calculated P-S-N curve.

• Journal of the Korea Society of Computer and Information
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• v.19 no.6
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• pp.93-100
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• 2014

The objective of this study is to propose the measurement system implementation method for the evaluation and measurement of the indoor-impulsive over 170 dB noise source. For the purpose of measuring impulse noise, design and implementation constructed followed subsystems of the testing center, microphone, ear simulator, head and torso simulator and so on. Measurement systems for the accuracy and reliability of impulse noise are implemented when measuring 3 ways of measurements method by the simultaneous measurement system design. For the accuracy and reliability of three mutually indoor-impulse noise measurements were compared, three kinds of measuring methods in accordance with the peak sound pressure level and octave band. Comparing the results of data, the indoor-impulse noise by analyzing a frequency characteristic was validated in difference for the statistical significance. Result are determined by the influence of the reflected wave. Therefore, the flexible size of the interior test site while interior impulse noise measurement system was constructed. Throughout this system can be affected by parameters that are the impulse noise source and the corresponding frequency-characteristic analysis to determine the spectrum of the reflected wave. And, in the near future, indoor impulse noise measurement systems for acquisition and analysis are utilized in useful data.

• The Journal of Engineering Geology
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• v.26 no.1
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• pp.143-152
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• 2016

To estimate the stability of a debris flow it is necessary to know the mass of surface soil, cohesion, slope, and friction angle. Given that the mass of surface soil is a function of soil thickness and mass density, it is important to obtain reliable estimates of soil thickness across a wide area. The objective of this paper is to estimate soil thickness using the elastic wave velocity with a new standard velocity. Tests are performed in debris-flow hazard areas, after which four profiles are selected to obtain the elastic wave velocity. Dynamic cone penetration tests are carried out to find the soil thickness at 18 points. The elastic wave velocity shows the area consists of 3~4 layers, and soil thicknesses are predicted by utilizing the new standard. The elastic wave velocity and dynamic cone penetration tests yield large differences in soil thickness. Therefore, this study shows that the new standard is useful not only in estimating soil thickness but also in improving the reliability of estimates of soil thickness.

• Journal of the Korean Wood Science and Technology
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• v.37 no.1
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• pp.48-55
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• 2009

In the glulam beam deflection it is necessary to check the reliability of theory formula, because of wood anisotropy and wood qualities (knot, slop of grain). In this experiment, when bending stress occurred on glulam, practice deflection of glulam measuring with AICON DPA-Pro 3D system were compared with prediction deflection calculated as substituting MOE through non-destructive testing and static MOE through bending test in differential equation of deflection curve. MOE using ultrasonic wave tester of laminae, MOE using natural frequencies of longitudinal vibrations ($E_{cu}$, $E_{cf}$), MOE using ultrasonic wave tester of glulam ($E_{gu}$) and MOE using natural frequencies of longitudinal vibrations ($E_{gf}$) were substituted in this experiment. When practice deflection measured by 3D system was compared with prediction deflection calculated with differential equation of deflection curve, within proportional limit the ratio of practice deflection and prediction deflection was similar as 1.12 and 1.14, respectively. Deflection using ultrasonic wave tester was 0.89 and 0.95, Deflection using natural frequencies of longitudinal vibrations was 1.07 and 1.10. The results showed that prediction deflection calculated by substituting using non-destructive MOE of glulam having anisotropy in differential equation of deflection curve was agreed well with practice deflection.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.48 no.4
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• pp.47-53
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• 2011

The Electric Multiple Unit (EMU) has many types of door system such as sliding door, plug door etc.al. according to customer's requirements. The sliding door is widely used in Korea but has weak point in the noise problem. In the low operation speed, the noise coming from outer side of the EMU is not an important factor. As the speed is higher than before, noise is increased and make a problem. The main cause of noise is the imperfect air tightness in the EMU. The plug door system has advantages for the noise reduction characteristic in the high speed area. We have been developing electric plug-in door. The door is controlled by Door Control Unit(DCU) following the order of Automatic Train Protection (ATP) that is a kind of train signalling system. DCU has to simultaneously open and close the doors and the operation of it is related to the passengers safety. So DCU is a safety device that is important to reliability and safety. DCU is composed of several devices of control, motor driving, Input/Output, communication and power. In this paper, we will describe the functions, characteristic, requirement, subsystem and test results of DCU used for the electric plug-in door.

• Journal of Life Science
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• v.29 no.6
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• pp.653-661
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• 2019

The first small interfering RNA (siRNA) therapeutics have recently been approved by the Food and Drug Administration in the U.S., and the demand for a new RNA therapeutics bioanalysis method-which is essential for pharmacokinetics, including the absorption, distribution, metabolism, and excretion of siRNA therapeutics-is rapidly increasing. The stem-loop real-time qPCR (RT-qPCR) assay is a useful molecular technique for the identification and quantification of small RNA (e.g., micro RNA and siRNA) and can be applied for the bioanalysis of siRNA therapeutics. When the anti-HPV E6/E7 siRNA therapeutic was used in preclinical trials, the established stem-loop RT-qPCR assay was validated. The limit of detection was sensitive up to 10 fM and the lower limit of quantification up to 100 fM. In fact, the reliability of the established test method was further validated in three intra assays. Here, the correlation coefficient of $R^2$>0.99, the slope of -3.10 ~ -3.40, and the recovery rate within ${\pm}20%$ of the siRNA standard curve confirm its excellent robustness. Finally, the circulation profiles of siRNAs were demonstrated in rat serum, and the pharmacokinetic properties of the anti-HPV E6/E7 siRNA therapeutic were characterized using a stem-loop RT-qPCR assay. Therefore, the stemloop RT-qPCR assay enables accurate, precise, and sensitive siRNA duplex quantification and is suitable for the quantification of small RNA therapeutics using small volumes of biological samples.

• Journal of Satellite, Information and Communications
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• v.12 no.4
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• pp.50-55
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• 2017

In this paper, the design of the RF system monitoring and control system of KUS (KASS: KASS Uplink Station) which constitutes KASS (Korea Augmentation Satellite System) is described. The Korean satellite calibration system is named KASS and aims to develop the SBAS system of the APV-1 level SoL service level and the CAT-1 test operation technology. Software and hardware development environment, function and algorithm of supervisory control device, structure of supervisory control device, and user interface were designed to implement KUS / RFS monitoring control device. We have secured the stability and reliability of the system by using the monitoring and control system design of the COMS (Communication Ocean & Meteorological Satellite) and the Korea Satellite 5A / 7 control system, which has already been used for the design of the surveillance control system. In addition, we have made it possible to provide the user interface according to the actual operator's request more conveniently.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.6
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• pp.903-909
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• 2018

In December 2017, the world's first LTE-R for 250km/h high-speed railway was commercialized with the opening of Wonju-Gangneung high-speed railway. LTE-R has many advantages such as voice and video call, high-speed data transmission, coverage redundancy and DU redundancy. But it requires efficient use of radio resources because of a limited bandwidth of 10MHz for UL and DL, respectively. The existing unicast scheme has limited high frequency efficiency so when the number of users increases, service is limited due to the network load, which could be a problem for LTE-R in terms of stability and reliability. On the other hand, the multicast scheme via eMBMS can provide stable service even if the number of video users is high. This paper derives the number of unicast scheme users considering the LTE-R network with LAB test and calculation result, and proposes implementation plans and considerations for eMBMS commercialization on LTE-R.