• The Korean Journal of Zoology
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• v.35 no.4
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• pp.405-413
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• 1992

등검은메뚜기(EuprepoceRneTmis shirak시 성충에서 면역작용에 관여하는 혈구로 확인된 Plasmatocvte와 granu10cvte 1 딪 granu10cyte 11의 형태와 미세구조적 특징을 광학현미경과 전자현미경을 이용하여 관찰하였다. Plasmatocyte는 방추형 또는 맡은 세포질돌기를 형성하고 있는 다형의 형태로 세포질에는 리소조옴과 식포가 존재하고 있었고, 식포의 융합에 의해서 형성된 multivesicular body가 관찰되었다. Granulocyte 1은 구형의 형태로 세포질에는 약 0.2-0.3 urn 크기의 과립을 함유하고 있었으며, 특히 원형질막 가장자리에 세포의 골격을 유지하는 미세소관 다발을 갖고 있었다. Granulocyte 11는 난형 또는 방추형의 형태로 세포질에는 약 0.5-0.8 Um의 과립이 세포질의 대부분을 차지하고 있었으며, 세포소기 관은 granu10cyte 1보다 덜 발달되어 있었다.

• Journal of Periodontal and Implant Science
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• v.28 no.4
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• pp.745-754
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• 1998

• 대한두경부종양학회:학술대회논문집
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• 1996.11a
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• pp.258.1-258.1
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• 1996

• 한국전자현미경학회:학술대회논문집
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• 2001.11a
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• pp.31-32
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• 2001

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.2
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• pp.312-315
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• 2000

To purify the macrophage phagocytic activity-enhancing component, ethanol-acetic acid (100 : 1, EA) extract of Korean wheat (Gobun wheat) and imported one (Australian Standard White, ASW) were fractionated with ethylacetate : methanol : $H_2O$(65 : 25 : 4, v/v/v), and identified by TLC and column chromatography. At least five fractions were separated from the EA extract of the wheats but amounts of fraction B, C and D were more in Gobun wheat than in ASW. The effects of all fractions on phagocytic activity were tested in macrophage J774 cells. Among the fractions, only fraction b of Gobun wheat showed significant increase of phagocytic activity against yeast.

• KSBB Journal
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• v.17 no.1
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• pp.26-32
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• 2002

The aerobic bacterium Xanthomonas campestris was cultivated continuously in a three-phase fluidized bed bioreactor to produce extracellular polysaccharide xanthan, Fluidized particles of 8.0 mm glass beads were used for disintegrating the large air bubbles even at high viscosities to improve the gas-liquid oxygen transfer rate. Xanthin productivity [kg xanthan/kg cell dry mass·h] and molecular weight increased, with dilution rate in the continuous xanthan fermentations. The specific xanthan productivities were not limited by the oxygen transfer rate and were much higher in the continuous cultivations than those predicted by the results of the batch xanthan fermentations.

• Applied Biological Chemistry
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• v.44 no.4
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• pp.215-218
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• 2001

To examine the expression of foreign protein in Polygonum tinctorium cells, plasmid pCAMBIA1302 encoding Green Fluorescent Protein(GFP) was used to transform the cells and the expression was confirmed using Western blot analysis. When the effect of sodium buryrate on the formation of GFP was examined, cell growth was retarded at the addition of 10 mM and was stalled at more than 15 mM. The amount of GFP production was increased by 15% when 5 mM of sodium butyrate was added at three-days after inoculation as compared to at 0-day. Moreover, when sodium butyrate was added at three-days after inoculation, the amount of GFP was increased by 50% at the addition of 5 mM of sodium butyrate as compared to 10 mM.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.10a
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• pp.243-244
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• 2002

연체동물, 복족강의 소화기관에 관한 연구는 20세기 초 Graham (1932, 1939)을 시작으로 많은 연구자들에 의해 형태 및 구조적 측면의 연구가 수행되어져 왔다(Graham, 1941,1964, 1973; Lutfy and Demian,1967; Bevelander, 1988). 복족류 소화관의 점막상피층을 구성하는 세포들의 종류 및 조직학적 특징에 대해서 Boer and Kits (1990)는 Lymnaea stagnaiis를 대상으로 소화관의 조직화학적인 방법과 미세구조적인 연구를 통하여 이들 소화관의 내강 상피조직이 섬모세포와 미세융모 그리고 다양한종류의 선세포들로 구성되어 있음을 보고한바 있다. (중략)

• Proceedings of the KOR-BRONCHOESO Conference
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• 1982.05a
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• pp.5.2-5
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• 1982

With the improvement of living standard and socioenvironmental change, esophageal stricture due to acetic acid, Hcl, insecticides and lye has led to decrease remarkably. Esophageal stricture has decreased because of immediate treatment and adequate management. However there are still sporadic incidents of esophageal stricture due to inadequate treatment and uncooperation of patients. The authors recently have experienced seven severe cases of esophageal stricture. All six patients were treated with 18-51 French Sippy esophageal dilating bougie. One patient was transfered to thoracic surgery department due to complete esophageal obstruction. Case 1. A 23 year old housewife who ingested Hcl for suicide. After Witzel's operation, she visited to dilate esophageal first constriction stircture due to swallowing difficulty postoperation 2 months later. We were treated successfully. Case 2. A case of esophageal stricuture in the second and third physiologic constriction part. The patient was 51 years old man who ingested lye accidently, and was dilated by bougination. Case 3. The patient was 43 year-old man who ingested acetic acid as a mistake and was treated inadequately at hopsital. Inspite of treatment, esophageal stricture developed at the third physiologic part. We are trying to dilate the esophagues now. Case 4. The patient was 55 year-old woman who had ingested Hcl for the purpose of suicidal attempt. 2 months later gastroduodenal anastomoisis due to pyloric region stenosis, the first physiologic constriction stricture was dilated successfully. Case 5. The patient was 41 year-old woman who ingested Hcl for suicide 4 months ago. There was indwelled orogastric tube for 1 month but esophageal stricture developed at the first and 3rd constriction part. She was treated by using a bougination. Case 6. An athlete 21 year old man, ingested acetic acid 2 spoonful per 3 days by purpose in order to soften the bone for last 14 months. There was complete esophageal obsturction in esophagogram and transferred to the thoracic surgery department. Case 7. A 3 year-old girl was ingested lye at a accident. She had a bougination for 16 months under the general anesthesia for dilation due to whole irregular esophageal stricture. She developed lower esophageal perforation, but healed eventually.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.159-162
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• 2000

It has been commonly known that cell growth is inhibited by the lack of dissolved oxygen and mass transfer inhibition of nutrients at stationary phase in fed-batch culture. In this study, Pluronic F-68 and oxygen vectors were added in Angelica gigas Nakai suspension culture in order to enhance cell growth in fed-batch culture. It was observed that the addition of 6%(w/v) Pluronic F-68 promoted cell growth up to 6.1% compared to control and that the use of 4%(v/v) n-hexadecane markedly enhanced cell growth up to 11.4%.