• Title/Summary/Keyword: 식물 축적

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토양전염성 병해 진단부터 철저히 - 난방제 토양전염성병의 발생생태와 방제방안(하) -

  • 박창석
    • The Bimonthly Magazine for Agrochemicals and Plant Protection
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    • v.12 no.1 s.100
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    • pp.81-88
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    • 1991
  • 토양전염성 병을 효과적으로 방제하기 위해서는 병의 진단에서 부터 전염원 동태 파악, 발병환경구명등 기본적인 연구자료의 축적위에서 현행 방제법들을 적절히 조화시킨 종합방제 체제를 확립하여야 한다.

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Studies on nickel uptake in transgenic Arabidopsis thaliana introduced with TgMTP1 gene encoding metal tolerance protein (TgMTP1 과발현 애기장대에서 Nickel 흡수 연구)

  • Kim, Donggiun
    • Journal of Plant Biotechnology
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    • v.42 no.4
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    • pp.409-413
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    • 2015
  • To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both $TgMTP_1$ (T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that $TgMTP_1$-overexpressing plants may be useful for phytoremediation of soil.

Induction of intercellular chitinase isozymes in bean and soybean leaves under stress (강낭콩과 대두 잎에서 세포간액 키틴분해효소의 분포와 유도)

  • Park, Ro-Dong;Cho, Yu-Young;Jhon, Deok-Young
    • Applied Biological Chemistry
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    • v.37 no.4
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    • pp.255-258
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    • 1994
  • Using the enzyme activity staining, we studied the induction and distribution of chitinase isozymes, pathogenesis-related proteins, in intercellular fluids of bean and soybean leaves under stress conditions. The chitinase in intercellular fluids was barely detected in healthy plant leaves. By treatment of ethylene, pathogen (Fusarium oxysporum), or wounding, only 34 kD intercellular endochitinase was induced in bean leaves, while 30 kD and 36 kD intercellular endochitinases were induced in soybean leaves.

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Effects of Municipal Sewage Sludge on Contents of Lead and Copper in Crop Plants. (경작지내(耕作地內) 도시하수(都市下水) Sludge 의 처리(處理)가 작물(作物)중 Cu 및 Pb 함량(含量)에 미치는 영향(影響))

  • Kim, Seong-Jo;Baek, Seung-Hwa;Chung, Dong-Jin
    • Korean Journal of Environmental Agriculture
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    • v.11 no.1
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    • pp.9-19
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    • 1992
  • To ditermine the accumulation of Cu and Pb in crops grown on Sludge-treated Soils, four crop plants, green onion(A Ilium ascalonicum L.), radish(Raphanus sativus L.), potato(Solanum tuberosum L.) and Chinese cabbage(Brassica pekinensis Rupr), were grown in greenhouses on soils treated with municipal sewage sludge at different levels of 0, 22.5, 45, and 90ton/ha as soil dry weight. The contant of heavy metals were analyzed in plant parts of the crop species and the statistical characteristics were investigated. The results were as follows: 1. Cu and Pb contents in experimental plants were increased in accordance with quanti of sludge applied. 2. Accumulation of Cu and Pb in leaves of plants was increased in order of radish

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Studies on Synthesis and Accumulation Pattern of Cyannogenic Glycosides in Sorghum Piants (Sorghum 식물에 있어서 Cyanogenic Glycosides의 합성 및 축적에 관한 연구)

  • ;G. Voigtlaender
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.2
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    • pp.121-126
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    • 1985
  • Phytotron and field experiments were conducted to determine the influence of morphological growth stage and environmental temperature on synthesis and accumulation pattern of cyanogenic glycosides in sorghum cv. Pioneer 931 and Sioux at Munich technical university from 1979 to 1980. Various growth stages of sorghum plants were grown in phytotron at 4 different temperature regimes of 30/25, 25/20, 28/18 and 18/8 degree C with 35,000 Lux over 13-h days. The results obtained are summarized as follows: 1. Cyanogenic glycosides in sorghum plants were shown to have a great synthetic rate at early growth stages. The highest concentrations of hydrocyanic acid (HCN) were found at 2-leaf stage with 2384 and 1800ppm (DM basis) for Pioneer 931 and Sioux respectively. The contents of HCN were, however, however decreased markedly as morphological development, which shows a value of 173ppm (Pioneer 931) and 70ppm (Sioux) at heading stages. 2. Changes of hydrocyanic acid in sorghum plants were positive correlated with leaf weight ratio and leaf area ratio ($P{\leqq}0.1%$), while plant height shows a negative correlation with HCN contents ($P{\leqq}0.1%$). 3. Cyanogenic glycosides were accumulated in young plants mainly in leaves. During the late maturities, the contents of HCN in leaves and stalks were shown, however, a similar distribution. 4. Synthesis rates of cyanogenic glycosides were increased under high temperature. Accumulated hydrocyanic acid in the plants was, however declined when temperature exceeded 30 degree C. 5. Synthesis rates of cyanogenic glycosides were affected by nitrogen reductase activity (NRA). The concentration of hydrocyanic acid in sorghum plants was associated with increasing of nitrate-N accumulation.

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Effects of sewage sludge Application on the Growth of Lettuce (Lactuca sativa L.) (상추의 생육에 대한 도시하수 슬러지의 영향)

  • Lee, Sang-Uk;Yoon, Wha-Mo;Oh, In-Hye
    • The Journal of Natural Sciences
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    • v.9 no.1
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    • pp.39-44
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    • 1997
  • This study was aimed to observe the effects of municipal sewage sludge as nutrients on the growth of Lettuce. The effects of heavy metal components of the sludge were also observed. Municipal sewage sludge from Taejon City were treated to the soil of PaiChai University Farm in order to make gradient of nitrogen concentration in soil among 6 experimental groups. Results are as follows ;The longest leaves and roots were produced in control pot, in which urea was supplied as nitrogen source to the soil. Any other sludge-treated groups produced shorter leaves and roots than control group, but the more sewage sludge treated the longer leaves and heavier vegetables were observed. No differences were observed among from 6 experimental groups in the view of N, K, Ca and Mg contents. But P contents in leaves from 6 groups varied. Zn content of Lettuce when was 65.8 mg/kg when it grew with sludge 200%. The groups treated with sludge 200% produced the vegetables of highest Zn contents. Accumulation of Zn, Mn and Cd was more heavier in Lettuce than in Altari radish. Accumulation of Zn, Mn, Cu and Cd in lettuce was not heavier than natural contents of those in market vegetables.

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The Effects of Abscisic Acid (ABA) on $Cd^{2+}$-induced Physiological Responses in Commelina communis L. (Abscisic acid가 $Cd^{2+}$에 의한 닭의장풀이 생리적 반응에 미치는 영향)

  • Lee Joon Sang
    • Korean Journal of Environmental Biology
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    • v.23 no.1
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    • pp.47-51
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    • 2005
  • Three-weeks old Commelina communis was transferred to Hoagland solution (Control, 100 μM Cd/sup 2+/, 100 μM Cd/sup 2+/ + 100 μM ABA, 100 μM Cd/sup 2+/+50 mM KCl) and grown for a week in the solution and then a number of physiological activities were investigated. In cases of Cd/sup 2+/ and Cd/sup 2+/ + ABA treatments, the growth of the plants was inhibited to 71 % and 81 %, respectively, when compared with the control, but there were no significant difference of plant growth between Cd/sup 2+/ and Cd/sup 2+/ + KCl- treated plants. In the treatments of Cd/sup 2+/, Cd/sup 2+/ + ABA and Cd/sup 2+/ + KCl total chlorophyll contents were reduced to 32%, 41% and 29%, respectively. In chlorophyll fluorescence experiments, Fv/Fm ratios were also reduced to 14∼20% and about 23%, respectively, according to the light intensity by Cd/sup 2+/ and Cd/sup 2+/+ABA-treated plants. Water stresses were increased by the treatment of Cd/sup 2+/, Cd/sup 2+/ + ABA and Cd/sup 2+/ + KCI. In Cd/sup 2+/ accumulation experiments Cd/sup 2+/ transport into the plant by ABA was not affected, but the accumulation of Cd/sup 2+/ into the roots was elevated to 13% when compared with the control. Cd/sup 2+/ transport into the root was markedly inhibited to 60% by KCl. Therefore, it could be concluded that ABA did not reduce the toxicities of Cd/sup 2+/, but enhanced Cd/sup 2+/ - induced toxicities and KCl showed no effect on Cd/sup 2+/ - induced toxicities.

Postchilling Accumulation of Superoxide in Cells and Chilling Injury in Rice Plant (Superoxide의 세포내 축적과 벼냉해의 발현)

  • Kim, Jong-Pyung;Hyun, Il;Jung, Jin
    • Applied Biological Chemistry
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    • v.30 no.4
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    • pp.364-370
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    • 1987
  • The $O_2^-$ level of the extract from young rice leaves, which was cold treated for 2 days and then placed at room temperature for a period of time significantly higher than that from tissues untreated. $O_2^-$ level in leaves was practically unchanged during cold treatment for 48 hours. But it started to increase to arrive at maximum in 8 hours, once the plants were placed under room temperature. The abnormal production of $O_2^-$ in mitochondria during postchilling process was interpreted as a biochemical consequence of accumulation of glycolysis product(s) in cytosol and/or NADH in mitochondrial matrix due to disruption of catabolic balance at low temperature. Mitochondria isolated from the chilling injured tissue was found to have lost considerably their respiratory activity. This fact may imply the involvement of intramitochondrial accumulation of $O_2^-$ in the inactivation of electron transport chain system. The observation that mitochondria in the presence of the $O_2^--producing$ enzymatic system (Xanthine/Xanthine oxidase) lost their respiratory activity supports this inference. It was also found in this work that Superoxide dismutase (SOD) is a substrate inducible enzyme, and that SOD is a possible protective agent in plant cell against chilling injury.

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