• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.257-261
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• 1998

The repeated subcultures of in vitro plant materials in wasabi became highly vitrified and the capacity for multiple shoot formation from the vitrified plant materials was very low. In order to improve the quality of in vitro propagated planting materials, the experiments were carried out using culture vessels capped with membrane filter(MF). When vitrified shoots were cultured on MS medium with 0.2mg/L BA in the vessels with MF or without MF for 60 days, the shoots in the vessels with MF did not vitrified. In contrast, the shoots grown in the vessels without MF vitrified at 65%. The stomates of vitrified leaves were circular and inflated, whereas those of normal leaves acclimatizated in the vessels with MF were ovate in shape. The hardened shoots were also cultured on MS media without sucrose containing 0.01mg/L IBA in vessels with(photoautotrophic culture) or without(control) MF. Sucrose was necessary for survival of the in vitro plantlets in the vessels without MF. After 20 days of culture, the shoots in the vessels without MF on the sucrose-free media turned yellow and died. But the shoots in the vessels with MF in the sucrose-free media produced a lot of roots. When shoots were cultured on MS medium with 2% sucrose containing 0.01mg/L IBA in the vessels with(photomixotrophic culture) or without(heterotrophic culture) MF, best growth occured in photomixotrophic culture.

• Korean Journal of Environment and Ecology
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• v.20 no.3
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• pp.299-310
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• 2006

The germination period, the germination under different saline concentration and temperature condition, the survival percent several halophytes in cross-transplanting experiment of marsh type and the soil environment factor of five dominant species were investigated on the halophytes population formation strategies on the salt marsh of Mankyeong river estuary from February to October 2005. In terms of germination, Suaeda japonica appeared first followed by Aster tyipolium Salicornia herbacda, Atriplex gmelini and Suaeda asparagoides. The germination percent on the humidify conditions and saline concentrations of halophytes were that Suaeda japonica germinated well under inundation conditions, high temperature and low salinity; Salicornia herbacda under high salinity; Aster tripolium and Atriplex gmelini under dry or inundation conditions, and Suaeda aspar agoides under dry conditions and high salinity. The survival percent in cross-transplanting experiment of five halophytes were high with Suaeda japonica in the low marsh; Salicornia herbacda, Aster tripolium and Atriplex gmelini in the mid marsh, Suaeda asparagoides in the high marsh. The soil properties were similar for the five halophytes, but soil texture appeared high in order of silt, sand and clay. These results suggested that population formation strategies of five halphytes. Suaeda iaponica was distributed in the low tidal marsh, Salicornia herbacda, Aster tripolium, Atriplex gmelini in the mid tidal marsh and Suaeda asparagoides in the high tidal marsh.

• Korean Journal of Plant Tissue Culture
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• v.21 no.4
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• pp.247-252
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• 1994

The effects of explant sources, plant growth regulators on callus induction and plantlet differentiation from leaf blade, petiole, and meristem tissue of Pelalgonium citrosa were investigated under illumination or in dark condition Leaf blade explants cultured on Murashige and Skoog's medium containing 2,4-D and kinetin did not form callus or organ. But those cultured on medium with NAA and BA produced callcus and shoots. Dark condition was more effective than light condition to callus induction and showed that some of shoot were differentiated directly from leaf blade explane. Callus proliferated vigorously on meristem tissue after 7 days of culture, and multiple shoots were obtained Sum callus on medium with 0.5 mg/L NAA and BA. Roots formed readily from about 80% of the shoots cultured on medium with 1.0 mg/L NAA. Regenerated plantlets regenerated had phenotypically normal leaves and roots.

• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.143-148
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• 2000

This study was carried out to know the optimum dose of gamma-ray for the induction of mutation in vitro and the characteristics of the mutants induced by gamma-ray in persimmon (Diospyros kaki Thunb.). The LD50 (50% lethal dose) for in vitro shoots of the cultivar, Nishimurawase was between 1 krad and 2 krad and about 1 krad for the cultivar, Ichikikeijiro. As the dose of gamma-ray increased, the length of shoots decreased and necrosis of buds increased. For the cultivar, Nishmurawase, 37.5∼58.3% shoots rooted and the rooting rate and the number of roots per shoot was low in high gamma-ray. The irradiated young plants which were grown in the growth cabinet for 6 weeks were shorter in shoot length and had more branches than non-irradiated plants. The survival rate of irradiated plants grown in the green house for 3 months was 33%, while 77% for control plants.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.9-22
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• 2009

Due to increasing concerns over issues with both water quantity and quality for turfgrass use, research was conducted to determine the response of five warm-season turfgrasses to deficit irrigation and to gain a better understanding of relative drought tolerance. St. Augustinegrass(Stenotaphrum secundatum [Walt.] Kuntze.) cultivars 'Floratam' and 'Palmetto', 'SeaIsle 1' seashore Paspalum(Paspalum vaginatumSwartz.), 'Empire' zoysiagrass(Zoysia japonica Steud.), and 'Pensacola' bahiagrass(Paspalum notatum Flugge) were established in lysimeters in the University of Florida Envirotron greenhouse facility in Gainesville. Irrigation was applied at100%, 80%, 60%, or 40% of evapotranspiration(ET). Evaluations included: a) shoot quality, leaf rolling, leaf firing; b) leaf relative water content(RWC), soil moisture content, chlorophyll content index(CCI), canopy photosynthesis(PS); c) multispectral reflectance(MSR); d) root distribution; and e) water use efficiency. Grasses irrigated at 100% and 80% of ET had no differences in visual quality, leaf rolling, leaf firing, RWC, CCI, and PS. Grasses irrigated at 60% of ET had higher values in physiological aspects than grasses irrigated at 40% of ET. 'Sealsle 1' and 'Palmetto' had a deeper root system than 'Empire' and 'Pensacola', while 'Floratam' had the least amount of root mass. Photosynthesis was positively correlated with visual assessments such as turf quality, leaf rolling, leaf firing, and sensor-based measurements such as CCI, soil moisture, and MSR. Reducing the amount of applied water by 20% did not reduce turfgrass quality and maintained acceptable physiological functioning.

• Journal of Ginseng Research
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• v.23 no.2 s.54
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• pp.74-80
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• 1999

Excised cotyledon segments of ginseng zygotic embryos cultured on MS basal medium without growth regulators produced somatic embryos near the basal excised portion at a high frequency. The frequency of somatic embryo formation on the segments declined along with advancing zygotic embryo maturity. In immature cotyledons, all the cells of the epidermis and subepidermis were smaller and more densely cytoplasmic than those in mature cotyledons, and from which multiple cells participated in embryogenic division to form somatic embryos with multiple cotyledons and fasciated radicles (poly-embryos). But in germinating cotyledons, only the epidermal cells were densely cytoplasmic and singularly competent to develop into somatic embryos resulting in single-embryos with closed radicles. This result means that the origin and development of somatic embryos is determined according to whether the cells participating in embryonic division are in a single state or a massive state relative to cotyledon maturity.

• The Journal of the Convergence on Culture Technology
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• v.9 no.5
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• pp.661-667
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• 2023

Five commercially available edible sweeteners are used as diet products because they can replace sucrose. In studies on the effects on animals and the human body, stability has been proven by excreting-oriented studies with characteristics of animal cells, and accumulation in small amounts has been ignored. On the other hand, plants can absorb, degrade, and accumulate foreign substances, so the effect of degradability and accumulation potential can be studied using plants. Metabolic effects in plants of commercially available saccharin and acesulfame potassium (Ace K) were tested using germinated barley and bean sprouts. In germinated barley and bean sprouts, saccharin and ace K showed inhibitory effects on plant growth in all organs from low concentrations in leaves, stems and roots. In addition, it can be observed that the symptoms of death appear clearly over time, so it can be seen that they are accumulated in the body of the plant. As the accumulated amount increases, the toxic effect increases and the plant reaches a state where it is unable to metabolize, turning black from the tip of the leaf and reaching a state of death. In order to remove the accumulated artificial sweetener, recovery was attempted by culturing in distilled water, but it acts as a substance that is not degraded and dies without avoiding toxicity. Saccharin and ace K cannot be excreted from the cell. Its toxic effects are thought to be persistent, inhibiting growth and eventually leading to cell death.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2000.10b
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• pp.89-93
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• 2000

광파장 및 조사시기에 따른 오이묘의 생육 반응을 검토하였다. 광원에 따른 오이 묘의 생육상태를 조사해본 결과 청색광, 적색광을 처리한 것이 생체중, 하배축 무게, 근중이 증가하면서 하배축의 신장이 억제되었고, 청색광과 적색광을 혼합하여 야간 12시간씩 조사하여 30일간 육묘해 본 결과 뿌리의 발육도 우수하고, 줄기와 잎의 생육 정도에서도 우량묘의 소질을 보였다. 청색광과 적색광을 혼합하여 야간 6시간과 12시간씩을 조사하여 30일간 육묘한 결과 하배축의 길이는 각각 60.0mm와 44.9mm였고, 하배축의 무게는 0.59g, 0.62g으로 나타나 12시간 조사구가 하배축의 신장억제효과가 큰 것으로 나타났다. 하배축의 엽록소 함량은 청색광과 적색광의 조사시간이 길어질수록 뚜렷이 증가하였고, 식물체도 진한 녹색을 나타냈다. 광처리구와 무처리구의 오이묘 하배축의 횡단면과 종단면을 검경하여 세포구조를 비교해 본 결과 광처리구의 횡단면 표피조직은 단층의 밀착된 세포로 되어 있으며, 바깥쪽 세포는 현저하게 두꺼웠다. 후각조직의 세포는 작고 부정형이며, 세포간극이 조밀하였으며, 세포구조도 치밀한 특성을 보였다. 종단면은 광처리구에서 세포가 짧고 세포간에 간극이 없는 반면 무처리구는 세포가 길고 뚜렷이 구분되지 않은 것이 관찰되어 대체로 광처리구는 무처리구에 비해 세포신장이 억제되는 대신 세포구조가 치밀하여 식물체의 도장이 억제된다는 것을 알 수 있었다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.248-248
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• 1994

목적 : 가래나무는 일부 민간방과 한방에서 암 치료의 목적으로 사용되고 있다. 아울러 이 식물을 대상으로 세포독성물질의 분리, 구조결정에 관한 연구는 이미 보고되어 있는 성분인 juglone이 새포독성을 나타낸다는 단편적인 보고만 있을뿐 전혀 되어있지 않은 상태이다. 본 연구는 국내에서 자생하는 가래나무 (Juglans mandchurica)로부터 세포독성물질을 분리, 구조결정함으로서 새로운 항암제 개발에 일차적인 자료를 제공하는 것을 그 목적으로 한다. 방법 : 가래나무 뿌리의 methanol 추출물을 hexane, chloroform, ethyl acetate. n-buthanol, water로 분획하고 세포독성을 측정하였으며, 이들중 ethyl acetate 분획으로부터 세포독성을 나타내는 물질들을 분리하였으며 현재 이들의 구조를 분광분석학적인 방법으로 결정하고 있다. 결과 : 현재까지 분리된 물질들은 약한 세포독성을 나타내고있으며, 그들 중 한물질의 구조를 결정하였다.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.6 no.6
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• pp.51-62
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• 1985

일반적으로 과수라 하면 사과, 배, 포도, 감, 밤 등 많은 종류가 포함되며 그들을 함께 묶어 해충관리에 대한 얘기를 하기는 어려움으로 여기서는 주로 사과, 배, 복숭아를 중심으로 많이 발생되는 주요 해충들에 대해서만 언급하기로 한다. 과수의 전 생육기간을 통하여 잎, 줄기, 뿌리, 과실 등을 가해하는 해충들을 대별하여 보면 나방류 해충으로 잎을 말고 가해하는 심식충류, 잎의 조직속으로 굴을 파고 들어가는 굴나방류, 성숙한 과실로부터 당분을 흡즙함으로서 피해를 입히는 과실 흡아류를 비롯하여 줄기속을 가해하는 유리나방, 하늘소 등의 줄기식입해충, 진딧물을 비롯한 각종의 잎 가해해충 그리고 곤충은 아니지만 일반적으로 편의상 곤충에 포함되어 취급되고 있는 응애류 등 각양각색이다. 우리나라에서는 이들 과수해충들에 대한 조사연구가 극히 미비한 상태로 주로 일본의 연구결과를 토대로 모든 방제대책을 적용하고 있는 실정이다. 특히 이들 과수해충들에 대해 연구하는 전문가가 국내에는 몇사람 되지않는 것이 심히 유감스러운 일이다. 본호에서는 이들 해충들중 여름철에 문제되는 종류들을 중심으로 그들의 개략적인 생태와 방제요령을 알아본다.