• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.63-68
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• 2002

The possibility for mass production of phytol, inhibitory diterpene against ACAT (Acyl-CoA: Cholesterol acyltransferase) was investigated by using callus culture of lettuce. The callus were induced from lettuce cotyledon explants on MS medium containing 0.5 mg.L$^{-1}$ NAA after 4 week's culture. Adventitious roots were formed from the explants on MS medium containing 0.5 mg.L$^{-1}$ IBA or 1.0 mg.L$^{-1}$ NAA. Adventitious shoots and roots were emerged from the callus when the callus was transferred to MS medium containing auxin alone, or with cytokinin. The plant growth regulators and their concentrations for the organogenesis were 1.0 mg.L$^{-1}$ NAA, 0.1 mg.L$^{-1}$ BA, 0.5 mg.L$^{-1}$ NAA with 0.1 mg.L$^{-1}$ kinetin, or 0.5 g.L$^{-1}$ 2.4-D with 1.0 mg.L$^{-1}$ kinetin. Analyses of chlorophyll contents showed that chlorophyll contents were higher in morphogenic calli than in non-morphogenic calli. However, the chemical analyses of gas chromatography indicated that phytol contents were not proportionate to the chlorophyll contents of callus. The content of phytol was higher in callus than in lettuce cotyledon.ledon.

• Journal of Plant Biotechnology
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• v.41 no.4
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• pp.216-222
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• 2014

This study was carried out to develop an efficient transformation protocol via particle bombardment with PLBs (protocorm-like bodies) in Phalaenopsis. To achieve this aim, osmoticum treatment and an increasing shooting chances in particle bombardment process were applied for this study. In addition, pCAMBIA3301: ORE7 vector which contains a herbicide-resistance bar gene as a selectable marker and ORE7 gene as a gene of interests were employed. With regard to the increasing chances of shooting in particle bombardment, double shooting was the best results with 1.5 ~ 2.5 times higher than those of a single or triple shooting treatment in the productioon of PPT (D-L-phosphinothricin)-resistant PLBs. However, regeneration rate of shoots in double shooting was not high as a single shooting. Further, double shooting showed 35 ~ 40% higher than that of a single shooting in the frequency of browning. Regarding effects of different osmotic treatments, combination of 0.2 M sorbitol with 0.2 M mannitol showed the best results in transformation efficiency, regeneration of transformants and reduction of browning. Putative transgenic Phalaenopsis plants were analyzed by PCR analysis and confirmed the presence of bar and ORE 7 gene. Also, real-time PCR was conducted by using 21 transgenic plants and showed only 4 plants had one copy of transgene; whereas, the other 17 plants had more than 2 copies of transgene. Transgenic phalaenopsis plants produced in this study were transferred to pots and flowered normally without morphological variations in flower and leaf.

• Journal of agriculture & life science
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• v.46 no.1
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• pp.71-81
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• 2012

This study was conducted to get basic information about making windbreak forest around Saemanguem reclaimed lands by analyzing the vegetation structure of 8 windbreak forests along the west coast of Korea and considering the methods of structural improvement. The total plants existing in the study areas were 25 families 40genus 43 species, and every tree layer consisted of only one tree species, Pinus thunbergii. There were 2 windbreak stands consisted of a species, and 2 consisted of multiple layer with a species. While, 4 windbreak stands consist of various species with several species. To make ecological system and functions of windbreak forests stabler, TL, STL and SL should be together in the vertical point, moreover, TL should be appeared at the in side and SL should be planted at the out side in the horizontal point. It is considered that mixed stand forest might be suitable for increasing the windbreak forest's functions and decreasing damage caused by disease and insects.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.40-40
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• 2019

시민인식은 하천관리를 포함한 공공정책 수립에 결정적 요소들 중 하나로 강조되고 있다. 따라서 하천관리체계의 변화에 따른 사회적 하천관리에 미치는 영향을 파악하고, 특히 우리나라 물관리 일원화 후 하천의 이용과 관리 및 정책에 대한 시민 인식을 조사하는 것은 향후 하천 정책 수립에 매우 중요하다. 본 연구의 목적은 하천의 이용, 관리(주체), 정책, 기후변화 등에 대한 시민의 관심도와 만족도를 조사하는 것이다. 이번 설문 대상은 전국의 남녀 4,500명(10대~60대)이었으며 패널 참여 방식으로 조사되었다. 설문 문항의 구성은 크게 하천 이용, 하천 관리, 그리고 물관리 일원화와 기후변화 등을 포함한 하천정책 등으로 3대 주제로 세부 문항들이 개발되었다. 설문조사의 주요 결과는 조사대상 시민들의 약 22.9%는 매주 하천을 방문하는 것으로 분석되었으며, 1년간 1번은 하천을 방문(이용)하는 시민들은 85.5%로 나타났다. 자주 방문하는 하천의 규모 혹은 위치는 중(中)하천이 42.2%로 가장 높았고, 대(大)하천은 23.9%, 소(小)하천은 17.5%, 도랑이나 개울은 15.5%로 나타났다. 기타(0.9%)로는 하천 외에도 물을 접할 수 있는 저수지, 인공하천 및 호수공원 등을 이용하는 것으로 나타났다. 하천 방문자 중 최소 2시간을 체류하는 시민 중 약 96.4%는 걸어서 하천을 접근하고, 지하철을 이용하여 하천을 접근하는 방문자는 최소 1시간에서 2시간 이상을 체류하는 것으로 조사되었다. 조사대상 시민들은 하천관리 중에서 접근 편의성(28.4%), 친수시설(자전거 도로, 산책로 등) 관리(24.2%), 수질관리(9.9%)로 만족하는 것으로 나타났다. 반면에 수질(27.4%, WQ(water quality))과 냄새 및 쓰레기(25.5%, OW(odor and waste))가 가장 높게 하천관리의 불만족도를 가지고 있었다. 기후변화 영향에 대한 시민들의 관심도는 WQ (29.6 %), OW (17.7%), 그리고 물부족 (15.1%)의 순서로 조사되었다. 하천의 바람직한 미래상에 대한 관심도는 깨끗한 수질의 하천(36.1%, WQ), 휴식과 산책을 즐길 수 있는 하천(22.9%), 다양한 동 식물의 생태서식공간(14.6%)의 순으로 나타났다. 또한 시민들은 하천관리 기관의 가져야할 역량은 '전문성'이 가장 중요하게 인식하고 있는 것으로 조사되었다. 우리나라 물관리 정책 중 가장 큰 문제로 우선순위 1~3순위 누적 조사 결과, 수질악화(79.9%, WQ), 하천 수생물 훼손(49.6%), 정부부처 간 협력부족(37.6%)으로 인식하는 것으로 나타났다. 끝으로 2018년 추진된 물관리 일원화 후 정부의 물관리 및 하천 정책에 대한 시민들의 관심도는 WQ (46.0%), 물공급 관리 (17.6%), 그리고 생태계 복원 (8.4%)의 순서로 조사되었다. 본 연구의 결론들 중 하나는 현재와 미래 하천에 대한 가장 높은 시민들의 수요는 깨끗한 수질과 생태서식공간이었으며 이는 향후 하천 정책 수립에 있어서 중요한 기초가 될 것으로 전망된다.

• Journal of Sericultural and Entomological Science
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• v.43 no.2
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• pp.83-87
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• 2001

This study was carried out to investigate the optimal medium and optimal dextrose concentration of 12 entomopathogenic fungi. The colonies of Cordyceps gracilioides, C. sp.(J23), Paecilomyces farinosus(J5), P tenuipes, Paecilomyces sp.(J4), Beauveria bassiana and B. brongniartii on SLA(silkworm larva agar) medium are similar to those on PDA(Potato dextrose agar) medium. On the contrary, the colonies of 5 fungi are very various according to its medium. The optimal medium for 1 1 fungi was SLA-A medium, which was better than PDA medium with regard to the mycelial growth. The optimal concentration on mycelial growth was superior to 6% for 6 fungi. When silkworm larvae was injected with P. tenuipes on SLA medium for fruiting body production. Infection rate, fruiting body formation rate, synnemata number and synnemata length were 91.3 $\pm$ 1.5%, 88.6 $\pm$ 2.3%, 56.6 $\pm$ 3.2 and 33.4 $\pm$ 2.88 ㎜ respectively. In economical analysis, the prototype medium can reduce 61.9% of production cost per 1 liter compared with PDA medium.

• Korean Journal of Heritage: History & Science
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• v.48 no.4
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• pp.62-73
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• 2015

The Tae-Jo Geonwolleung is the tomb of the first king Tae-Jo of the Joseon Dynasty in the complex of the Royal Tombs of the Joseon Dynasty which is listed in World Heritage, and it contains various types of stone properties. The material of the stone properties consist of a single type of medium-grained biotite granite, however, each properties have different intensity and types in deterioration phenomena according to their locations and purposes. The major deterioration types of the stones are analyzed as physical decomposition and biodeterioration. The exfoliation, breaking-out and granular decomposition are widespread types of the deterioration throughout the entire burial mound stone properties and surrounding stone properties. On the other hands, the colonization of mosses and plants, and contamination by foreign materials are found more frequent in the burial mound stone properties as these stones are in contact with the soil of the tomb mound and moisture in the soil helped biodeterioration. It is suggested that anti-biology treatment and physical reinforcement are applied to the deteriorated stones to prevent further damage on the stone components of the tomb.

• Journal of Life Science
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• v.29 no.5
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• pp.524-529
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• 2019

Medicinal plants resources are becoming important assets since their usages have been expanded to the development of functional foods for human health, cosmetics and pharmaceutical industries. However, their phylogenetic origins and names are different from each country and quite often they are mixed each other resulting in the confusion for consumers. Particularly when they are very similar based on their morphological characteristics and distributed, it is extremely difficult to differentiate their origins even by specialists. Therefore, identification of each plant species is important for standardizing herbal medicine. Thistle is a medicinal and perennial plant. Obtaining information about the genetic diversity of plant populations is highly important for conservation and germplasm utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from chloroplast genomic sequences to identify distinct Korean-specific thistle species via high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the trnL-F and matK chloroplast intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different country.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.2
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• pp.139-145
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• 2021

Plant-derived exosome-like nanovesicles (PELNs) are known to include various biological activities and possess high biocompatibility. Because PELNs can influence immune responses, cell differentiation, and proliferation regulation, they can be applied in multiple industries. However, the studies on the skin physiological of exosome-like nanovesicles derived from plant callus are insignificant compared to nanovesicles derived from mammalian cells. In this study, callus was induced from apple fruit (Malus domestica), and exosome-like nanovesicles (ACELNs) were isolated for improving skin barrier and anti-aging. The yield of ACELNs was 6.42 × 10⁹ particles/mL, and the particle size was ranged from 100 to 200 nm. HDF cells and HaCaT cells were concentration-dependent, increased in cell, and decreased in cytotoxicity. The cornified envelope formation was significantly increased compared to the control group. The COL1A1 expression and the FBN1 expression in HDF cells were increased. In addition, the ACELNs promoted collagen biosynthesis in UVA-irradiated HDF cells. These results might be considered as potential materials that could improve skin barrier and prevent skin aging.

• Journal of Environmental Impact Assessment
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• v.29 no.6
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• pp.430-440
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• 2020

The application contents, process, and its limitations are discussed for the setting of Korean legal guides & criteria for water cycle and ecological condition in development project of land use by thorough comparison and examination of prerequisites and credits of water cycle and soil+vegetation by USA's SITES (Sustainable Sites Initiative). In the case of SITES, due to the implementation procedure operated as a non-governmental independent assessment system by Green Business Certification, Inc, the natural condition of water cycle and soil-vegetation items-the key element of ecosystem services can be quantitatively assessed, well along with its legal and institutional guidelines and regulations. On the other hand, in the case of Korea, as a part of the national certification procedure for green building, the ecological area ratio system still have very limited role as an only amenity resource in the creation of artificial green spaces and insufficiency of management system for rain water. In conclusion, it was understood as an urgent situation in necessary for prompt establishment of site's sustainability certification system at the national level, based on management of water circulation and natural soil & vegetation in developed area with consideration of various land uses and types of development projects.

• Journal of the Korean Society of Earth Science Education
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• v.15 no.2
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• pp.299-309
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• 2022

The purpose of this study is to categorize specific factors and characteristics of positive science experience by qualitatively analyzing the positive science experience cases in the memories of pre-service elementary school teachers. For this purpose, 101 essays written by pre-service teachers on the theme of 'my enjoyable science class experience' were analyzed. The collected data were analyzed using an inductive analysis method, and as a result, the characteristics of positive science experience cases in the memories of pre-service teachers were categorized into 4 categories and 12 topic groups. Specifically, 'experience of exploratory activities using special materials', such as long-term raising and observation of animals and plants, experiments using edible materials, and using special experimental tools had a positive effect on scientific experience. In addition, 'experience of activities emphasizing scientific inquiry' such as emphasizing observation activities, experiments accompanied by clear experimental results, making activities, and using various materials also had a positive effect on science experience. In addition, 'student-centered activity experience' such as strengthening student autonomy, emphasizing collaboration between students, and performing science activities outside of the curriculum also had a positive influence on the science experience. Lastly, 'positive influence of teachers', such as teachers' encouragement and enthusiasm, helped to positively recognize science.