• 한국생물공학회:학술대회논문집
• /
• 2001.11a
• /
• pp.99-102
• /
• 2001

Recombinant Saccharomyces cerevisiae strains harboring various copy numbers of hirudin gene were developed to study dependency of hirudin expression level on its gene copy number. A linear relationship between the copy number of hirudin expression cassette and hirudin expression level was observed up to 10 copies. A double <5-integration vector truncated wi 디 1 the unnecessary bacterial genes before yeast transformation showed a four-fold increase in transformation efficiency and a 1.3-fold enhancement in hirudin expression level compared with a single <5 system. Gratuitous hirudin expression strain was developed by disrupting the GALl gene of S. cerevisiae. Glucose that was fed in a limited manner effectively supported cell growth and hi겨din expression by the gratuitous strain. Effects of methanol concentrations on hirudin production in recombinant Hansenula polymorpha were investigated in continuous and fed-batch cultures. At a steady-state of continuous culture, an optimum methanol concentration of 1.7 g/L was determined at a dilution rate of 0.18 $h^{-1}$ with 1.8 mg/L ${\cdot}$ h hirudin productivity.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.19 no.1
• /
• pp.9-17
• /
• 2001

GPS is an efficient system in surveying and car navigation. but it is difficult to catch minimum number of satellite when it is hindered by obstacle such as city area. GLONASS system doesn't have perfect constellation yet, but it has many similarities with GPS system in principle of point positioning and signal system. so, it is expected to be much efficient when it is used with GPS system. For the purpose of this, the coordinates of GPS and GLONASS system, the quality of time and frequency was investigated, and the algorithm of point positioning was made. also, the efficiency of GPS/GLONASS combination was presented by analysing the precision of 3D point positioning using C/A code and Yuma satellite orbit information.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.11 no.10
• /
• pp.1977-1985
• /
• 2007

The character recognition technique is one of the artificial intelligence and has been widely applied in the automated system robot HCI(Human Computer Interaction), etc. This paper introduces the character set and the representative character that can be used in the recognition of the mage ROI. The character codes in this ROI include the digit, symbol, English and Hereat etc. We proposed the efficient multi-classifier structure by combining the small-size classifiers hierarchically. Moreover, we generated each small-size classifiers by delta-bar-delta learning algorithm. We tested the performance with various kinds of images and achieved the accuracy of 99%. The proposed multi-classifier showed the efficiency and the reliability for the mixed character code set.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2012.04a
• /
• pp.17-20
• /
• 2012

최근 인터넷의 발달과 함께 웹을 기반으로 하는 클라이언트-서버 분산 구조의 웹서비스 시스템 구조가 점차 확산되고 있다. 게다가, 최근에는 스마트폰을 이용한 스마트폰 애플리케이션이 대중화 되면서, 웹 서비스의 활용이 점차 확대되는 추세이다. 웹을 기반으로 클라이언트와 서버 사이에 통신을 하기 위해서는 원격 프로시저를 정의한 인터페이스가 규정되어야 하며, 기존에는 W3C에서 정의한 WSDL를 사용하여 웹서비스를 기술하곤 하였다. 그러나, 이와 같은 기존의 웹서비스 기술 및 사용방법은 그 구성이 복잡하고 오버헤드가 큰 이유로 널리 활용되지 못하였다. 최근에는 스마트폰이 대중화 되면서 REST 웹서비스의 활용이 확산되는 추세다. SOAP 기반 웹서비스에 대해서는 서비스 조합에 대해서 충분히 다루어 졌으며, 어느정도 정리된 연구분야이다. SOAP 웹서비스는 기계가 인식하기 쉽도록 엄격한 규약과 인터페이스를 정의한 것이기 때문이다. 하지만, REST 웹서비스 조합 방법에 대해서는 현재까지도 충분히 연구되지 않았다. REST 웹서비스는 사람이 사용하기 편리하도록 설계된 기술이고, 그 조합에 대해서는 표준에서 관여하지 않기 때문이다. 본 논문에서는 REST 웹서비스의 기존의 소켓(socket)을 이용한 서버-클라이언트 통신 방법 대신에, REST 웹서비스를 활용하는 서버-클라이언트 스마트폰 앱개발 방법을 제안한다.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.16 no.2
• /
• pp.355-362
• /
• 2021

Since coaxial magnetic gears use non-contact power transmission, friction, wear, noise, and heat generated in the power transmission process of existing mechanical gears can be minimized. Currently, research for application to various industries is being actively conducted, but among the characteristics of coaxial magnetic gears, the problem of rapidly decreasing torque density at a high gear ratio was discussed. This paper proposes a direction for multiple gear combination using low gear ratio coaxial magnetic gears with high torque density. In order to confirm the effectiveness of the method, the torque density was compared with a single high gear ratio model, and the combination and design direction of multiple coaxial magnetic gears were shown.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2022.05a
• /
• pp.278-278
• /
• 2022

2020년 집중호우로 인하여 우리나라 전국에 걸쳐 약 2,000여 곳의 산사태, 토석류가 발생하였고 약 1,217ha의 피해 면적이 발생하였다. 피해지역의 특히 생활권 중심의 사면과 계류의 관리 필요성이 높아지고 있다. 산림청 산사태정보시스템에서는 토양함수지수가 80% 도달 시 주의보, 100% 도달 시 경보를 발령하는 대국민 서비스를 제공하고 있다. 본 연구에서는 토층의 깊이에 따른 함수비 분포에 따라 토석류의 발생 가능성에 대한 분석을 수행하고자 하였으며, 토양함수는 기상 수치모델에 의한 예측 강우 자료를 활용하였다. 예측 강우 모델은 토석류가 주로 발생하는 여름철 집중호우 시기인 남서풍을 고려하여 도메인을 구성하였고 산림의 증발산 및 토양수분 모의 정확도 향상을 위해 임상도와 토지피복도를 사용하여 보정하였다. 토층내 토양수분의 함량은 토질에 따라 그 특성이 다르기 때문에 토질과 관련한 주제를 이용하여 토양정보를 활용하였다. 내부마찰각, 점착력, 단위중량, 밀도, 지질도, 지형경사, 표고, 유효토심에 대한 정보를 구축하여, 예측강우에 따라 토층의 수분 함량을 추정하여 붕괴 발생 가능성을 분석하였다. 2006년 평창지역에서 발생한 토석류에 대하여 수행하였으며 토층의 심도는 0.5~1m 범위의 분포에 대하여 체적함수에 따른 실제 토석류 발생에 대한 검증을 수행하였다.

• Korean Journal of Microbiology
• /
• v.49 no.1
• /
• pp.1-7
• /
• 2013

During meiosis, genetic recombinants are formed by homologous recombination accompanying with the programmed double-strand breaks (DSBs) and strand exchanges between homologous chromosomes. The mechanism is generated by recombination intermediates such as single-end invasions (SEIs) and double-Holliday junctions (dHJs), and followed by crossover (CO) or non-crossover (NCO) products. Our study was focused on the analysis of meiotic recombination intermediates (DSBs, SEIs, and dHJs) and final recombination products (CO and NCO). We identified these meiotic recombination intermediates using DNA physical analysis under HIS4LEU2 "hot spot" system in budding yeast, Saccharomyces cerevisiae. For DNA physical analysis, when the hot spot locus is recognized by restriction enzyme from synchronous meiotic cells, the fragmented DNA that are forming recombination intermediates can be detected and quantified through Southern hybridization analysis. Our study suggests that this system can analyze the structural change of recombination intermediates during DSB-SEI transition, double-Holiday junctions and crossover/non-crossover products in meiosis.

• The Journal of the Korea Contents Association
• /
• v.9 no.10
• /
• pp.32-39
• /
• 2009

Semantically-operated services, which is different from Web services or semantic Web services with semantic markup, can be defined as the services providing search function or reasoning function using ontologies. It performs a pre-defined task by exploiting URI, ontology classes, and ontology properties. This study introduces a method for pipelining semantically-operated services based on a semantic broker which refers to ontologies and service description stored in a service manager and invokes by user constraints. The constraints consist of input instances, an output class, a visualization type, service names, and properties. This method provides automatically-generated service pipelines including composit services and a simple workflow to the user. The pipelines provided by the semantic broker can be executed in a fully-automatic manner to find a set of meaningful semantic pipelines. After all, this study would epochally contribute to develop a portal service by ways of supporting human service planners who want to find specific composit services pipelined from distributed semantically-operated services.

• Journal of Life Science
• /
• v.21 no.12
• /
• pp.1726-1731
• /
• 2011

Human Stem Cell Factor (hSCF) is a cytokine that binds to the c-Kit receptor and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. To produce the human Stem Cell Factor (hSCF) recombinant protein, we constructed a germline transgenic silkworm using the piggyback vector. The expression of the hSCF gene was driven by the Drosophila heat shock protein 70 (dHsp70) promoter. 3XP3 promotor-driven EGFP was used as a marker which allowed us to rapidly distinguish the transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 1,020 eggs of bivoltin silkworms, Keomokjam. We obtained approximately 22 G1 broods that were EGFP-positive. The expression of the hSCF gene in the transgenic silkworm was analyzed by SDS-PAGE and immunoblotting. Also, analysis of insertion sites into the silkworm genome using inverse PCR showed that exogenous DNA was inserted into the transgenic silkworm genome. These results show that successfully constructed transgenic silkworm expresses the hSCF recombinant protein.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2019.05a
• /
• pp.533-536
• /
• 2019

Recombinant baculoviruses are widely used to express heterologous genes in cultured insect cells. Recombinant baculoviruses can serve as gene-transfer vectors for expression of recombinant proteins in a wide range of mammalian cell types. Baculovirus system has significant benefits in view of safety, large-scale, and high level of gene expression. In this study, baculoviral vectors which were reconstructed from pOPINEneo-3C-GFP vector, were recombined with cytomegalovirus (CMV) promoter, green fluorescent protein (GFP), and p53 with NcoI and XhoI. These recombinant vectors were infected with various cells and cell lines. The baculovirus vector thus developed was analyzed by comparing the metastasis and expression of the recombinant genes with conventional vectors. These results suggest that the baculovirus vector has higher efficiency in metastasis and expression than the control vector. This work was supported by a grant from Mid-Career Researcher Program(NRF-2016R1A2B4016552) through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future Planning(MSIP).