• Title/Summary/Keyword: 시간혼성

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Rapid Detection for Shiga Toxin Type 1 (Stxl) by Using Two-Step Ultra-Rapid Real-Time (URRT) PCR (초고속 이단계 PCR에 의한 Shiga 독소 타입 1의 신속 검출법)

  • Kim, Il-Wook;Kang, Min-Hee;Kwon, Soon-Hwan;Cho, Seung-Hak;Yoon, Byoung-Su
    • Korean Journal of Microbiology
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    • v.44 no.3
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    • pp.203-211
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    • 2008
  • Rapid detection-method for Shiga toxin type 1 that was produced from Shiga toxin-producing Escherichia coli (STEC) was developed by two-step ultra-rapid real-time (URRT) PCR. The specific primers were deduced from 80 bp stable region of stx type 1 (stxl) gene among various informations of STEC strains. URRT PCR is a microchip-based real-time PCR using 6 ${\mu}l$ of reaction volume with extremely short denaturation step and annealing/extension step (1 sec, 3 sec, respectively) in each cycle of PCR. Using the stx1-specific URRT PCR, 35 cycled PCR were finished in time of 6 min and 38 see, also measured 7 min and 28 see including melting temperature (Tm) analysis. The detection-limit of stxl-specific URRT-PCR was estimated until 3 colony forming units / PCR with products with stable Tm at $81.42{\pm}0.34^{\circ}C$. In the applications to various STEC strains and contaminated genomic DNAs, stx1-specific URRT-PCR were tested and shown that it would be expected an useful method for the rapid detection of stx1-coded STEC strains.

A Study on the Pitch Search Time Reduction of G.723.1 Vocoder by Improved Hybrid Domain Cross-correlation (개선된 혼성영역 교차상관법에 의한 G.723.1의 피치검색시간 단축에 관한 연구)

  • Jo, Wang-Rae;Choi, Seong-Young;Bae, Myung-Jin
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.59 no.12
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    • pp.2324-2328
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    • 2010
  • In this paper we proposed a new algorithm that can reduce the open-loop pitch estimation time of G.723.1. The time domain cross-correlation method is simple but has long processing time by recursive multiplication. For reduction of processing time, we use the method that compute the cross-correlation by multiplying the Fourier value of speech by it's complex conjugate. Also, we can reduce the processing time by omitting the bit-reversing of FFT and IFFT for time-frequency domain transform. As a result, the processing time of improved hybrid domain cross-correlation algorithm is reduced by 67.37% of conventional time domain cross-correlation.

Implementation of ray tracing simulator for extracting sound field parameters (음장파라미터 추출을 위한 음선추적 시뮬레이터의 구현)

  • Lee, Deok-Su;Seong, Goeng-Mo
    • The Journal of the Acoustical Society of Korea
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    • v.14 no.3
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    • pp.82-89
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    • 1995
  • A sound field simulator is constructed to obtain the sound field paramaters such as the magnitudes and directions of early reflections with moderate efforts. The proposed simulator is based on the hybrid ray tracing method that traces rays reached the listener position and convert them to image sound sources. By this approach, we can obtain the directional impulse response relatively easily with minimum casts. Simulation experiment results of several performace places are reported to how the versatility of the proposed simulator system.

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On a System Realization of Automatically Recovering the Hook-switch of the Telephone System for a Howler Sound- (하울러음 발생시 훅-스위치 자동복구 시스템의 구현 -하울러음 검출알고리듬에 관하여-)

  • 배명진;정영창
    • The Journal of the Acoustical Society of Korea
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    • v.11 no.6
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    • pp.53-60
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    • 1992
  • 사용자의 부주의로 전화기의 전화기의 송수화기가 방치되면 가입자는 통화요금의 불이익을 받거 나 외부로 부터의 전화호출을 받을 수 없다. 따라서 우리는 가입자라인을 통해 하울러음이 발생되면 전 화기의 훅-스위치를 자동으로차단하는 전화기 시스템을 개발하고자 한다. 이러한 시스템에서는 하울러 감지기의 성능이 시스템의 성능을 좌우하기 때문에 이의 검출법이 필요하다. 따라서 본 논문에서는 하 울러음에 대해 시간영역에서의 특징과 주파수 영역에서의 장점을 살린 혼성영역검출기법을 세로 제안하 였다. 이 하울러음 검출기에 대한 평가는 주변환경으로서 몇가지의 음성신호와 가우시안잡음을 섞어 수 행하였고, 그 결과는 6dB 이하의 신호대 잡음비에 대해 검출오류가 1%이하로 우수하게 얻어졌다.

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Non-synchronized Sampling Techniques for DMT-based xDSL Modems (DMT 기반의 xDSL 모뎀의 비동기식 샘플링 방식)

  • 이미현;김재권;백종호;유영환;조진웅;조용수
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.12B
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    • pp.2141-2153
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    • 2000
  • 본 논문에서는 DMT 기반의 xDSL 시스템의 수신단에서 발생하는 샘플링 위상 옵셋과 샘플링 주파수 옵셋에 의한 타이밍 오류를 분석한 후, 디지털 수신기에서 이를 보상하기 위한 비동기식 샘플링(full digital PLL) 방식을 제안한다. 기존의 논문에서는 DMT 방식의 xDSL 시스템에서 샘플링 위상 옵셋을 delay-rotor 특성을 이용한 주파수영역 위상 회전기로 보상하는 비동기식 샘플링 방식을 제안한 바 있다. 그러나 수신단에서 샘플링 시 존재하는 타이밍 오류로 인해 저역통과 필터링된 수신신호는 더 이상 delay-rotor 특성이 성립하지 않아 성능이 크게 저하된다. 본 논문에서는 샘플링 위상 옵셋을 완벽하게 보상할 수 있는 데이터 구간의 환형 컨벌루션화(circular convolution) 방식을 제안한다. 또한 샘플링 위상 옵셋과 샘플링 주파수 옵셋이 동시에 존재하는 경우 이를 보상할 수 있는 개선된 시간/주파수 혼성영역 보상방식을 제안한다. 또한 추가의 오버헤드를 사용하지 않고 샘플링 위상 옵셋과 샘플링 주파수 옵셋을 보상할 수 있는 시간영역 보상방식을 제안한다. 마지막으로 DMT 방식의 ADSL 시스템에 본 논문에서 제안된 비동기식 샘플링 방식들을 적용하여 모의실험을 통해 성능을 분석하고 기존의 방식과 비교하여 성능의 우수성을 확인한다.

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Hybrid Water Treatment of Tubular Ceramic MF and Photocatalyst Loaded Polyethersulfone Beads : Effect of Water Back-flushing Period and Time (관형 세라믹 정밀여과와 광촉매 첨가 PES 구의 혼성 수처리 : 물 역세척 주기와 시간의 영향)

  • Park, Jin Yong;Park, Sung Woo;Byun, Hongsik
    • Membrane Journal
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    • v.23 no.4
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    • pp.267-277
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    • 2013
  • The effect of water back-flushing period (FT) and water back-flushing time (BT) was compared with the previous study of nitrogen back-flushing in viewpoints of resistance of membrane fouling ($R_f$), permeate flux (J), and total permeate volume ($V_T$) in hybrid process of tubular ceramic microfiltration and PES (polyethersulfone) beads loaded $TiO_2$ photocatalyst for advanced drinking water treatment. As FT decreasing, Rf decreased, but J and $V_T$ increased. Turdity treatment efficiency was the maximum at NBF (no back-flushing) and increased a little as FT decreasing in both water and nitrogen back-flushing. Organic matter treatment efficiency was the maximum at FT 4 min in water back-flushing, but increased as FT decreasing in nitrogen back-flushing. As BT increasing, Rf and resistance of reversible membrane fouling ($R_{rf}$) decreased, but J and $V_T$ increased. The turdity treatment efficiency was almost constant beyond 98% in water back-flushing, but increased as BT increasing except NBF in nitrogen. The organic matter treatment efficiency was the maximum at BT 6 sec in water back-flushing, but increased as BT increasing except NBF in nitrogen. The $V_T$ was the maximum at BT 30 and FT 2 min, and optimal condition was BT 30 sec per FT 2 min in this experimental range.

Effect of Water Back-flushing Time and Polypropylene Beads in Hybrid Water Treatment Process of Photocatalyst-coated PP Beads and Alumina Microfiltration Membrane (광촉매 코팅 폴리프로필렌(PP) 비드와 알루미나 정밀여과막의 혼성 수처리 공정에서 물역세척 시간 및 PP 비드의 영향)

  • Park, Jin Yong;Kim, Sunga;Bang, Taeil
    • Membrane Journal
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    • v.26 no.4
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    • pp.301-309
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    • 2016
  • The effects of water back-flushing time (BT) and photocatalyst-coated polypropylene (PP) beads were investigated in hybrid water treatment process of alumina microfiltration and the PP beads in this study, and compared with the previous study with alumina ultrafiltration membrane and the same PP beads. The BT was changed in the range of 6~30 s with fixed 10 min of back-flushing period (FT). Then, the BT effects on resistance of membrane fouling ($R_f$), permeate flux (J) and total permeate volume ($V_T$) were observed during total filtration time of 180 min. As longer BT, $R_f$ decreased and J increased dramatically; however, $V_T$ was the maximum at BT 10 s. The treatment efficiency of turbidity was high beyond 99.0%, and the BT effect was not shown. The treatment efficiency of organic matters was the highest value of 89.0% at no back-flushing (NBF), and increased as longer BT. The optimum input concentration of the PP beads was 20 g/L in the viewpoint of membrane fouling; however, the optimum PP beads of the previous study was 40 g/L. The treatment efficiency of turbidity and organic matters were the maximum at 30 g/L of the PP beads; however, those of the previous study with alumina ultrafiltration membrane and the same PP beads were the highest at 40 g/L.

Hybrid Water Treatment of Tubular Alumina MF and Polypropylene Beads Coated with Photocatalyst: Effect of Nitrogen Back-flushing Period and Time (관형 알루미나 정밀여과와 광촉매 코팅 폴리프로필렌 구의 혼성 수처리: 질소 역세척 주기와 시간의 영향)

  • Park, Jin Yong;Choi, Min Jee;Ma, Jun Gyu
    • Membrane Journal
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    • v.23 no.3
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    • pp.226-236
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    • 2013
  • The effect of $N_2$ back-flushing period (FT) and time (BT) was compared with the previous result used PES (polyethersulfone) beads loaded with titanium dioxide photocatalyst in hybrid process of alumina microfiltration and PP (polypropylene) beads coated with photocatalyst in viewpoints of membrane fouling resistance ($R_f$), permeate flux (J), and total permeate volume ($V_T$). The reason of nitrogen back-washing instead of the general air back-washing method is to minimize the possible effect of oxygen included in air on water quality analysis. As decreasing FT, $R_f$ decreased and J and $V_T$ increased. Treatment efficiency of dissolved organic matters (DOM) was 82.0%, which was the higher than 78.0% of the PES beads result. This means that PP beads coated with photocatalyst was the more effective than PES beads loaded with photo-catalyst in the DOM removal. As increasing BT, the final $R_f$ decreased and the final J increased, but $V_T$ was the maximum at BT 15 sec. The average treatment efficiency of turbidity did not have any trend as changing BT. As BT increasing from 6 sec to 30 sec, the treatment efficiency of DOM increased 11.8%, which was a little higher than the result of PES beads.

Hybrid Water Treatment of Tubular Ceramic MF and Photocatalyst Loaded Polyethersulfone Beads : Effect of Nitrogen Back-flushing Period and Time (관형 세라믹 정밀여과와 광촉매 첨가 PES 구의 혼성 수처리 : 질소 역세척 주기와 시간의 영향)

  • Hong, Sung Tack;Park, Jin Yong
    • Membrane Journal
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    • v.23 no.1
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    • pp.70-79
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    • 2013
  • The $N_2$ back-flushing period (FT) and time (BT) were investigated in hybrid process of ceramic microfiltration and PES (polyethersulfone) beads loaded with titanium dioxide ($TiO_2$) photocatalyst for advanced drinking water treatment in viewpoints of membrane fouling resistance ($R_f$), permeate flux (J), and total permeate volume ($V_T$). As decreasing FT and increasing BT, $R_f$ decreased and J increased, and finally the maximum $V_T$ could be acquired at FT 10 min and BT 30 sec. In FT effect experiment, treatment efficiencies of turbidity and dissolved organic matters (DOM) were the highest at no back-flushing (NBF) because of dramatic membrane fouling. As result of BT effect, the treatment efficiencies were the maximum at BT 30 sec, which was different with the FT result. Because the photocatalyst beads could be cleaned effectively as decreasing FT and increasing BT, turbidity treatment efficiency increased a little from 95.4% to 97.5% as decreasing FT, and from 95.9% to 98.5% as increasing BT. Also DOM treatment efficiency increased from 70.8% to 80.6% as decreasing FT, and from 75.1% to 85.8% as increasing BT. The optimal condition, where the treatment efficiencies and $V_T$ were the maximum, should be FT 10 min and BT 30 sec in our experimental range.

On a Pitch Alteration Method by Time-axis Scaling Compensated with the Spectrum for High Quality Speech Synthesis (고음질 합성용 스펙트럼 보상된 시간축조절 피치 변경법)

  • Bae, Myung-Jin;Lee, Won-Cheol;Im, Sung-Bin
    • The Journal of the Acoustical Society of Korea
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    • v.14 no.4
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    • pp.89-95
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    • 1995
  • The waveform coding technique has concerned with simply preserving the waveform shape of speech signal through a redundancy reduction process. In the case of speech synthesis, the waveform coding with high sound quality is mainly used to the synthesis by analysis. However, since the parameters of this coding are not classified into either excitation or vocal tract parameters, it is difficult to applying the waveform coding to the synthesis by rule. In order to apply the waveform coding to the synthesis by rule, the pitch alteration technique is required in prosody control. In this paper, we propose a new pitch alteration method that can change the pitch period in waveform coding by scaling the time-axis and compensating the spectrum. This is relevant to the time-frequency domain method were the phase components of the waveform is preserved with a little spectrum distortion of 2.5 % and less for 50% pitch change.

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