• Title/Summary/Keyword: 스트레스정도

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Ethanol Induced Leucocytic and Hepatic DNA Strand Breaks Are Prevented by Styela clava and Styela plicata Supplementation in Male SD Rats (알코올로 인한 흰쥐의 백혈구 및 간 DNA 손상에 미치는 미더덕과 오만둥이 분말의 보충섭취 효과)

  • Kim, Jung-Mi;Park, Hae-Ryoung;Lee, Seung-Cheol;Park, Eun-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.10
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    • pp.1271-1278
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    • 2007
  • In this study, the ability of Styela clava or Styela plicata to reduce ethanol-induced hepatotoxicity and hepatic and leucocytic DNA damages was evaluated. Twenty four male SD rats were given 25% ethanol containing water (ad lib, p.o.) and divided into 3 groups; ethanol treated control group (EtOH), ethano1+3% S. clava (EtOH+SC), and ethano1+3% S. plicata (EtOH+SP). After 6 weeks, the supplementation of S. clava reduced the plasma ALT, ALP and LDH activities significantly (p<0.05), while S. plicata induced significant decrease in the plasma LDH activity only. The comet assay was employed to quantify the alcohol-induced DNA damage in rat hepatocytes and leucocytes. A significant protective effect on hepatic and leucocytic DNA damages was observed in S. clava or S. plicata supplemented groups compared to the EtOH control group. The hepatic DNA damage was correlated positively with plasma ALP and LDH activities. These results demonstrated that S. clava or S. plicata supplementation protected alcohol-induced hepatic and leucocytic DNA damage.

Effect of Phytoncide on Porphyromonas gingivalis (P. gingivalis에 대한 피톤치드의 항균효과)

  • Kim, Sun-Q;Shin, Mi-Kyoung;Auh, Q-Schick;Lee, Jin-Yong;Hong, Jung-Pyo;Chun, Yang-Hyun
    • Journal of Oral Medicine and Pain
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    • v.32 no.2
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    • pp.137-150
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    • 2007
  • Trees emit phytoncide into atmosphere to protect them from predation. Phytoncide from different trees has its own unique fragrance that is referred to as forest bath. Phytoncide, which is essential oil of trees, has microbicidal, insecticidal, acaricidal, and deodorizing effect. The present study was performed to examine the effect of phytoncide on Porphyromonas gingivalis, which is one of the most important causative agents of periodontitis and halitosis. P. gingivalis 2561 was incubated with or without phytoncide extracted from Hinoki (Chamaecyparis obtusa Sieb. et Zucc.; Japanese cypress) and then changes were observed in its cell viability, antibiotic sensitivity, morphology, and biochemical/molecular biological pattern. The results were as follows: 1. The phytoncide appeared to have a strong antibacterial effect on P. gingivalis. MIC of phytoncide for the bacterium was determined to be 0.008%. The antibacterial effect was attributed to bactericidal activity against P. gingivalis. It almost completely suppressed the bacterial cell viability (>99.9%) at the concentration of 0.01%, which is the MBC for the bacterium. 2. The phytoncide failed to enhance the bacterial susceptibility to ampicillin, cefotaxime, penicillin, and tetracycline but did increase the susceptibility to amoxicillin. 3. Numbers of electron dense granules, ghost cell, and vesicles increased with increasing concentration of the phytoncide, 4. RT-PCR analysis revealed that expression of superoxide dismutase was increased in the bacterium incubated with the phytoncide. 5. No distinct difference in protein profile between the bacterium incubated with or without the phytoncide was observed as determined by SDS-PAGE and immunoblot. Overall results suggest that the phytoncide is a strong antibacterial agent that has a bactericidal action against P. gingivalis. The phytoncide does not seem to affect much the profile of the major outer membrane proteins but interferes with antioxidant activity of the bacterium. Along with this, yet unknown mechanism may cause changes in cell morphology and eventually cell death.

Efficacy of deferoxamine on paraquat poisoning (파라쿼트 중독 환자에서 deferoxamine의 치료 효과)

  • Huh, Jin Won;Jegal, Yangjin;Hong, Sang-Bum;Oh, Yeon Mok;Shim, Tae Sun;Lim, Chae-Man;Lee, Sang Do;Kim, Woo Sung;Kim, Dong Soon;Kim, Won Dong;Koh, Younsuck
    • Tuberculosis and Respiratory Diseases
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    • v.62 no.2
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    • pp.113-118
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    • 2007
  • Background: Paraquat is known to induce oxidant injury that results in multiorgan failure and lung fibrosis. Iron has been considered to play a key role in paraquat-induced oxidant lung injury. This study examined the effect of deferoxamine, an iron-chelating agent, in the treatment of paraquat poisoning. Methods: From September, 2001 to April, 2005, 28 patients with paraquat poisoning who were admitted at a medical intensive care unit of a University-affiliated hospital, were enrolled in this study. Sixteen patients were treated according to the paraquat poisoning treatment guidelines and 12 received an intravenous infusion of deferoxamine in addition to the treatment guidelines. Results: There were no differences between the two groups in terms of age, gender, severity of paraquat poisoning, and the time elapsed from ingestion to presentation at hospital. There was no difference in overall mortality between the two groups but the incidence of respiratory failure in the deferoxamine group was higher than in the conventional group(4/7 versus 0/9, p=0.019). Conclusion: Deferoxamine seems to have no clinical benefit compared with the conventional treatment.

A Study on Effects of Subjective Perception to Nutrient Intake and Mental Health of Korean Adolescents: Using the Korea National Health and Nutrition Examination Survey (청소년들의 체형인식에 따른 영양소 섭취실태 및 정신건강 - 국민건강영양조사 자료를 이용하여 -)

  • Shin, Sanghee;Shin, Woo-kyoung;Kim, Yookyung
    • Journal of Korean Home Economics Education Association
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    • v.29 no.1
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    • pp.93-109
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    • 2017
  • The objective of this study was to investigate the effects of the subjective perception to the nutrient intake and mental health of Korean adolescents by the residual method. This study was based on data from the 5th and 6th Korea National Health and Nutrition Examination Survey (2010-2014) of the 2,961 total subjects(male 1,548, female 1,413, aged 12 to 19 years). The nutrient intake was collected by using the 24 hr recall method and mental health and BMI were collected by the health interview survey and physical examination research. An analysis of the relationship between the subjective perception and BMI showed that the proportion of those with a distorted perception of their body type was the highest among adolescents with normal weight(p for trend, <.001). Especially, regarding the subjective body type, the proportion of adolescents who perceived themselves overweight was significantly higher among females as compared to that among males (p for trend, <.001). An analysis of nutrient intake status against the subjective perception showed that the group of adolescents who perceived themselves overweight had the lowest scores on the index of nutritional quality (INQ), nutritional adequacy ratio (NAR) and mean adequacy ratio (MAR) (p for trend, <.001). With respect to the daily intake of major nutrients, adolescents who perceived themselves overweight consumed the lowest amounts of total energy (p for trend, <.01) and carbohydrate (p for trend,<.001). An analysis of the mental health status of the adolescents showed that those who perceived themselves overweight had the highest levels of stress recognition and depression (p<0.01). In conclusion, the adolescents' subjective perception is associated with their nutrient intake and mental health. Therefore, some educational programs are desirable to help the adolescents to have their desirable subjective perception and to increase their satisfaction with their body type.

Healthy Korea 2010 : Role of the Health Educator (Healthy Korea 2010추진과 보건교육 인력 활용 전략)

  • Choi, Eun-Jin
    • Proceedings of The Korean Society of Health Promotion Conference
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    • 2004.10a
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    • pp.89-109
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    • 2004
  • The Korean Government has produced the Health Plan 2010 aimed at setting up healthy Korea objectives, policies on preventing chronic diseases, reshaping the country's health and medical infrastructure. The policy goal targets the people's healthy life expectancy at 75 by 2010, and includes healthy life practice measures including health education, health improvement services, and disease management measures, in achieving the objectives. Also, the plan provides life cycle-based health improvement and disease prevention services, as well as pushes ahead with projects with greater ripple effects in each area. To this end, the government is simultaneously pushing to operate an experts-centered health promotion committee and establishing the infrastructure including the augmentation of national health improvement funds. Through its Health Plan 20 I 0, the Korean Government will exert efforts to achieve its policy objectives as addressed in the measures by enhancing the national potential health and providing systematic disease prevention services.

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Community Analysis of Oribatid Mites (Acari : Oribatida) in the Process of Needle Leaf Decomposition in Korean Pine (Pinus koraiensis) Forest of Namsan and Kwangreung (남산과 광릉지역의 잣나무림에서 낙엽분해과정에 관련된 날개응애 군집분석)

  • 배윤환
    • The Korean Journal of Soil Zoology
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    • v.6 no.1_2
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    • pp.25-31
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    • 2001
  • Oribatid mite communities in the process of litter decomposition were analyzed In Korean pine (Pinus koraiensis) forests of Namsan and Kwangreung, which were supposed to be under different environmental selective pressures. Oribatid mites were collected bimonthly from the litter bags (mesh size 0.4 mm, 1.7 mm) which was set up in the forest floor of study sites. This study had been carried out from Jan., 1997 to Sept., 1998. Species abundance of Kwangreung (mesh size 1.7 mm) was significantly higher than that of Namsan (mesh size 1.7 mm), but total no. of species did not exceed 30 species in all study sites. Concerning body length, medium sized oribatid mites (0.3-0.7mm) were more abundant than small ( < 0.3 mm) and large ( > 0.7 mm) sized mites. In Kwangreung, species whose body lengths were 0.2 mm to 0.5 mm were major group. However, a little larger species than Kwangreung's major group were dominant in Namsan. Sorenson similarity index and cluster analysis suggested that there were qualitative and quantitative differences in species composition in Namsan and Kwangreung. More species were collected in May through September than the other sampling times, but the pattern was rather different between first year and second year. Newly immigrant species were high in May in the first year and many of them regained on next year. Diversity indices suggested that species diversity of Kwangreung was higher than that of Namsan. Nearly 70% of total individual abundance was occupied by several dominant species in Namsan and Kwangreung . In the litter bags of mesh size 1.7 mm, the most dominant species was Trichogalumna nipponica in Namsan and Kwangreung, but in the litter bags of mesh size 0.4 mm in Kwangreung it was Ramusella sengbuschi which is smaller than T. nipponica. And important species related to litter decomposition were selected as follows; T. nipponica, Epidamaeus coreanus, Scheloribates latipes, Ceratozetes japonicus, Ramusella sengbuschi, Eohypochthonius crassisetiger, and Cultroribula lata.

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Effect of buchu (Allium tuberosum) on lipid peroxidation and antioxidative defense system in streptozotocin-induced diabetic rats (부추가 Streptozotocin 유발 당뇨쥐의 지질과산화와 항산화방어체계에 미치는 영향)

  • 송영선;정현실;노경희;조혜연;박지영;최춘연;권태완
    • Journal of Life Science
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    • v.13 no.3
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    • pp.333-342
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    • 2003
  • The pathogenic effort of high glucose, possibly in concert with fatty acids, is mediated to vascular complications of diabetes via increased production of reactive oxygen species(ROS), reactive nitrogen species(RNS), and subsequent oxidative stress. This study was carried out to investigate the suppressive effect of buchu(Allium tuberosum) on oxidative stress in streptozotocin(STZ)-induced diabetes in Sprague Dawley male rats. The effect of buchu supplementation (10%) on lipid peroxidation, and antioxidative defense system in blood and liver was compared among normal rats fed basal diet(normal) and diabetic rats fed basal diet(DM-control) or 10% buchu-supplemented diet(DM-buchu). Diabetes was experimentally induced by the femoral muscle injection of 50 mg STZ per kg of body weight. Animals were sacrificed after 4 wks of experimental diets feeding. The induction of diabetes by STZ elevated the level of lipid peroxidation represented by thiobarbituric acid-reactive substances(TBARS) and conjugated dienes in plasma, LDL, liver, and erythrocytes. 10% buchu-supplemented diet significantly reduced the levels of conjugated dienes in erythrocytes(p<0.05) and lowered TBARS in liver and LDL to the levels of control. Induction of diabetes by STZ elevated Mn-superoxide dismutase(Mn-SOD) activity and lowered activities of glutathionine reductase(GSH-red) and glutathionine peroxidase(GSH-px). Catalase activity was not affected by the induction of diabetes by STZ. However, buchu supplementation to diabetic rats significantly elevated catalase activity(p<0.05) and slightly elevated GSH-px and GSH-red activities in liver. GSH levels of blood and liver were lowered or not changed by induction of diabetes by STZ, respectively, while buchu supplementation to diabetic rats significantly elevated hepatic GSH level (p<0.05). In conclusion, it can be concluded that buchu might be a food source to attenuate oxidative stress in diabetic patients by inhibiting lipid peroxidation, by increasing hepatic GSH level, and by inducing anti-oxidative enzyme systems.

Physiological Responses of Wearing Safety Helmet with Cooling Pack in Hot Environment (머리 냉각시의 인체생리반응 - 안전모 착용을 중심으로 -)

  • Choi, Jeong-Wha;Park, Joon-Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.31 no.6 s.165
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    • pp.955-965
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    • 2007
  • Safety helmets are used widely in various industries by workers since they are legally required to wear them. However, thermal discomfort is one of the major complaints from helmet users. To relieve this problem, frozen gelled packs can be considered for use inside the helmets. In this paper, tests were performed on humans to evaluate the physiological strains of wearing safety helmets and to investigate the effects of using frozen gelled packs inside the helmets. Experiments were conducted in a climatic chamber of WBGT $33{\pm}1^{\circ}C$ under four differed experimental conditions: 1) not wearing a safety helmet(NH); 2) wearing a safety helmet with frozen gelled pack A(HA); 3) wearing a safety helmet with frozen gelled pack B(HB); and 4) wearing only a safety helmet(OH). The results were as follows. First, when comparing NH with OH, physiological responses such as $\bar{T}_{sk},\;T_r$, HR and sweat rate were significantly higher in OH and subjective sensations were reported as less hot and more comfortable than NH(p<.05). Second, in regard to the frozen gelled packs inserted inside the safety helmets, some physiological responses in HA were different from those in HB, according to the two different types of packs. HA was hotter, more uncomfortable and less exhausted than HB. However, result from both HA and HB were lower than those from OH in terms of temperature and humidity inside safety helmet, sweat rate, $T_r$ increase, heat storage(p<.05). When wearing safety helmets with frozen gelled packs, it was shown that heat strain can be alleviated. These results are expected to help millions of workers who complain that wearing safety helmets is uncomfortable and messy.

Arthroscopic ACL Reconstruction Using Fresh-Frozen Achilles Allograft -Clinical results, Recovery of sports activity- (아킬레스 동종건을 이용한 전방십자인대 재건술후 임상적 결과와 운동력 회복 평가)

  • Chun Churl Hong;Ha Dae Ho;Kim Dong Chul;Kim Hyun Jun
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.1 no.1
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    • pp.31-36
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    • 2002
  • Purpose : ACL (anterior cruciate ligament) reconstruction using achilles allograft was done for whom ACL injured person in recreational sports activity. The purpose of this study was to evaluate the clinical results and return to their sports activity in these patients. Materials and Methods : ACL injured 56 amateur athletes who had experienced sports 3 times a week more than 5 years, reconstructed with Achilles allograft, and it was analyzed subjective and objective parameter, Tegner scoring, Telos stress arthrometer, Lysholm Knee Scoring System and modified Feagin scoring system. The average age was 25 years old (range: 18$\~$49), the average follow up period was 15 months (range: 12$\~$19). Morbid sports were football (29 cases), basket ball (14 cases), badminton (5 cases), tennis (3 cases), squash (2 cases) and otherwise (3 cases). Result : The mean Lysholm Knee Scoring System was improved to 88.2 from 60. Telos arthrometer in anterior stress test revealed 2.3 mm improved from 7.1 mm. The modified Feagin scoring system showed 50 cases (89$\%$) with excellent and good results. We had obtained 12 cases (21$\%$) of Tegner score VI, 32 cases (57$\%$) of score V, 20 cases (35%$\%$ of score IV, 3 (5.3$\%$) cases of score III. Conclusions : Reconstruction of anterior cruciate ligaments can restore stability sufficient to allow sports activity in ACL injured patients, but it’s difficult to achieve 'normal' sports activity. So we will have to solve the reasons of this dissatisfaction at furthermore.

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Workplace Friendship and Organizational Effectiveness of Dental Hygienists (치과의료기관 근무자들의 프렌드십과 조직효과성 관계 연구)

  • Yoo, Youngsuk;Seo, Youngjoon;Kim, Sungho
    • Journal of dental hygiene science
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    • v.12 no.6
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    • pp.644-651
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    • 2012
  • This study purports to measure the level of work friendship in dental clinic and examines the friendship's effect on the organizational effectiveness. Data were collected from workers who worked in dental clinic located in Seoul and Gyeonggi areas by self-administered questionnaires from early in October till lately in September, 2009 through direct interview and e-mail. Among 250 questionnaires, 240 responses were returned, and 17 copies with an inaccurate answer were excluded. Finally 223 responses were analyzed through SPSS program. The study revealed that the work friendship in dental clinic has enormous influence on job satisfaction, occupational commitment, intent to leave, stress etc. The results imply that the managers of the dental clinics need to create an organizational climate which emphasizes on a good relationship among members and have them take part in various committees or informal activities.