• Microbiology and Biotechnology Letters
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• v.44 no.4
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• pp.529-534
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• 2016

Bioethanol was produced using the separate hydrolysis and fermentation (SHF) method with macroalgal polysaccharides from the seaweed, Undaria pinnatifida as biomass. This study focused on the pretreatment, enzymatic saccharification, and fermentation of yeasts in co-culture. Ethanol fermentation with 14.5% (w/v) seaweed hydrolysate was performed using the yeasts, Saccharomyces cerevisiae KCTC 1126 alone, Pichia angophorae KCTC 17574 alone, and their co-cultures with the yeasts either adapted to mannitol or not. Among the combinations, the co-culture of non-adapted S. cerevisiae and P. angophorae adapted to mannitol showed high bioethanol production of 12.2 g/l and an ethanol yield ($Y_{EtOH}$) of 0.41. Co-culture in the SSF process was employed in this study, to increase the ethanol yields of 35.2% and reduction of 33.3% in fermentation time. These results provide suitable information on ethanol fermentation with marine seaweeds for bioenergy production.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.32 no.4
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• pp.331-339
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• 1996

It is most important to understand the behaviour of fish in case of the betterment of the current fishing gear and methods or the development of the conditioning by acoustic sound in marine ranching. This investigation has been attempted to provide for the prediction of the response action of fish to acoustic sound. The experimental fish was conditioned with sound and bait. As the acoustic sound for stimulus, the pure tone of sine waveform at the frequency of 200Hz was used. This pure tone was determined from previous investigation about hearing ability of walleye pollock Theragra chalcogramma. The fork length of walleye pollock used in this experiment was 385~450mm. The conditioning proceeding was recorded in the video tape recorder. Frequency of appearance in the feeding area was analyzed with computer and video tape recorder. The position of fish was tracked using the mouse cursor and picture mixed on the superimpose board. The response of conditioned fish to sound stimulus was appeared in the 8th day firstly. The conditioned fish remembered the stimulus sound for 4 days. Average frequency of appearance in the feeding area during the 30 seconds sound projection or 1 minute after the sound stimulus was 51%, and was higher than before it.

• Ocean and Polar Research
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• v.26 no.3
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• pp.433-438
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• 2004

Stress response to the seawater acclimation in coho salmon (Oncorhynchus kisutch) smolt was investigated. Salt concentration of rearing water was gradually increased for 3 days from freshwater to seawater (30 ppt salt level). The changes of serum concentrations of cortisol as a primary stress indicator, and as secondary indicators, glucose (GLC), lactate (LAC), triglyce.ide (TG), cholesterol (CHOL), sodium ion $(Na^+)$, chloride ion $(Cl^-)$ and enzyme activities (alanine aminotransferase, ALT: aspartate aminotrasferase, AST; lactate dehydrogenase, LDH) were quantified during the acclimation experiment. Among them, cortisol, LAC, TG, CHOL, ALT, AST concentrations showed rapid increase at the first exposure to the 10ppt salt level (day 1), and began to decrease to the constant values after day 2 of adaptation at 20ppt salt level. However, LDH concentration tended to decrease during the whole experimental period. $Na^+\;and\;Cl^-$ showed slight decrease at day 1, and increased to a little bit higher values after day 2 rather than those in freshwater. All the fishes started on taking a food after day 4 of seawater adaptation. From these results, to reduce osmotic shock inducible stress to fish in seawater acclimation, gradual increase of salt levels is recommended.

• The Journal of the Acoustical Society of Korea
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• v.25 no.1
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• pp.7-13
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• 2006

Fisheries policy change from catching to farming requires more intensive consideration for aquaculture industry. The oceanic farm is a desirable cost effective aquaculture method. However. in odor to gather fish in the oceanic farm, eating sound or any attracting sound should be radiated through underwater loudspeaker. In this Paper, it has been found in literature that the frequency range responding to fish is about 16Hz to 13kHz but sensitive frequency range is about 150Hz to 2kHz and sound pressure level is about 100dB to 150dB reference $1{\mu}Pa$. Therefore, frequency range and output sound level of designed underwater loudspeaker has been specified as 150Hz to 3kHz and 100dB to 145dB reference $1{\mu}bar$. respectively To verify the stability and the endurance to the pressure of 40m water depth, manufactured underwater loudspeaker was examined before sea trial in manufactured water pressure tank which gives a maximum of 10 atmospheric Pressure. We experimented on acoustic characteristic with manufactured underwater loudspeaker under water depth of 10m.

• Phonetics and Speech Sciences
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• v.1 no.4
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• pp.11-15
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• 2009

The aim of this study was to identify production difficulties of Korean learners of English in their articulation of English bilabial stops /p, b/ and labiodental fricatives /f, v/. Sixty non-sense syllables and twelve words were produced three times by nine graduate students. Test scores were measured from the score board made by FluSpeak, a speech training software program, which was designed for English pronunciation practice and improvement. Results show that 1) the subjects had lower scores in producing /p, b/ than /f, v/ from all positions, and 2) subjects had lower scores in medial (inter-vocalic) position than in initial (pre-vocalic) position and in final (post-vocalic) position when they produced /p/, /b/, /f/, and /v/. The results suggest that on the whole, Korean learners of English have much difficulty in producing /b/ and that they also have more articulatory problems in intervocalic than in the other positions when they produce these bilabial stops and labiodental fricatives.

• The Transactions of the Korea Information Processing Society
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• v.5 no.10
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• pp.2498-2511
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• 1998

본 논문에서는 질의화상을 위한 칼라의 위치묘사 키워드와 칼라 키워드를 칼라특징으로 매핑하여 검색할 수 있는 내용기반 화상 검색 방법을 제안하고 이를 구현하였다. 칼라 키워드는 화상의 칼라 특징을 사용하여 칼라 세그먼트 프리미티브로부터 정의되고, 위치 묘사 키워드는 칼라 영역 정보를 사용하여 위치 세그먼트 프리미티브로부터 정의된다. 정의된 각 칼라 키워드 프리미티브는 화상의 칼라특징으로 매핑되어 저장된 참조화상의 6x6 블록의 칼라 특징과 비교하게 되고 유사도 순치 묘사 키워드와 칼라 키워드 검색의 정확도를 측정하였고, 화상검색 실험결과, 평균 recall/precision이 0.72/0.80를 보임으로써 내용기반 화상 데이터 검색에 제안된 방법이 유용함을 보였다.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.05a
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• pp.80-82
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• 2003

어군의 음향에 대한 행동을 알아내는 것은 어업에 있어서 중요한 일로, 많은 어종의 청각특성을 파악하는 것이 필요하며 많은 연구자들이 다양한 실험방법으로 측정하여 왔으며, 그 결과 어류도 어느 주파수대의 음을 감지하는 능력이 있다든가 음원 방향을 탐지하는 능력을 가진다는 것 등이 밝혀지고 있다. 이처럼 어류의 청각능력을 어업생산에 응용하기 위한 하나의 시도로서 음과 사료에 의한 학습, 즉 수중음향을 이용하여 어군의 유집행동을 유발하고, 어군을 학습시켜 어군의 행동영역, 섭이행동 등을 제어하기 위해서는 어느 주파수의 음을 어느 정도의 음압으로 방성할 때 어류가 들을 수 있는지, 어떤 음이 학습음으로 가장 효과적인지, 어느 정도의 기간동안 학습을 시켜야 학습효과가 명확하게 나타나고 가장 오랜 동안 학습효과를 유지시킬 수 있는지 등을 구명하는 일이 매우 중요하나 이에 관해서는 참돔, 넙치, 연어, 송어 등 극히 일부의 어종에 대해서만 연구되고 있는 실정이다. (중략)

• The Journal of Korean Medical History
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• v.21 no.2
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• pp.79-93
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• 2008

Through researching 鄕藥救急方, the oldest medical text published in Korea in existence known to date, a study from a Traditional Korean Medicine perspective was conducted. This study aims to observe the treatment of oral, glottal, labial, and dental diseases starting from 統一新羅時代 to the Koryo dynasty from a historical perspective and also find out what clinical significance it withholds. "鄕藥救急方" proposes methods of nurturing the teeth and in doing so displays the tendency of Traditional Korean Medicine of putting much weight on preventing diseases and nurturing the body. "鄕藥救急方" considers the oral, glottal, labial, and dental diseases as not only fundamental problems in itself but also treats them from a perspective that they are results of disharmony among the five viscera and the six bowels. The treatment methods mentioned in "鄕藥救急方" are directly cited in "鄕藥集成方" of early Chosun dynasty. In "東醫寶鑑" of mid-Chosun dynasty, however, changes and modificatios are made to the prescriptions.

• Journal of fish pathology
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• v.36 no.2
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• pp.263-275
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• 2023

Chum salmon (Oncorhynchus keta) is a species which returns to Korea for spawning and was produced as seed production at the Fisheries Resources Agency located in Uljin-gun, Gyeongsangbuk-do to preserve the species. However, farmed chum salmon showed symptoms of bacterial infection. Therefore, in this study, bacteria were isolated to identify the causative agent from chum salmon in October 2021. The isolated bacteria were identified based on the sequences of 16S rDNA, rpoD (RNA polymerase sigma factor σ⁷⁰), and vapA (A-layer) genes. Also, salinity-growth curve, biochemical characterization, antibiotic susceptibility test, and pathogenicity analysis were performed in four strains. As a result, four isolated strains were identified as Aeromonas salmonicida subsp. salmonicida. Additionally, the bacterial strains showed a decrease in growth as the salt concentration increased in the medium. All of the isolated strains exhibited γ-hemolysis, and the same biochemical properties. In the antimicrobial susceptibility test, all strains showed an inhibition zone of 40 to 44 mm for oxolinic acid, flumequine, and florfenicol. Pathogenic factors were assessed by RT-PCR at the mRNA level, and found that the four strains expresses the outer membrane ring of T3SS (ascV), inner membrane ring of T3SS (ascC), vapA, enterotoxin (act), and lipase (lip) genes which are well known to significantly contribute to the pathogenicity of A. salmonicida. The results of this study can be used as basic data to prevent A. salmonicida subsp. salmonicida occurring in sea-chum salmon in the future.

• Microbiology and Biotechnology Letters
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• v.44 no.2
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• pp.145-149
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• 2016

Thermal acid hydrolysis pretreatment of Kappaphycus alvarezii was carried out with 12% (w/v) seaweed slurry and 180 mM H₂SO₄ at 140°C for 5 min. Utility of the thermotolerant yeast Kluyveromyces marxianus KCTC7150 was evaluated with respect to cell growth and ethanol fermentation at 40°C was close to optimal for enzymatic hydrolysis. This could lead to the integration of both the saccharification and fermentation processes. The levels of ethanol production by simultaneous saccharification and fermentation (SSF) with non-adapted and adapted K. marxianus KCTC7150 were 9.1 g/l with an ethanol yield (Y_EtOH) of 0.24 and 10.2 g/l with an ethanol yield (Y_EtOH) of 0.27 at 156 h, respectively. The two-phase SSF process was employed in this study to improve the efficiency of ethanol fermentation. Adapted K. marxianus KCTC7150 using the two-phase SSF process produced 13.5 g/l with an ethanol yield (Y_EtOH) of 0.35 at 96 h. Development of the two-phase SSF process could enhance the overall ethanol fermentation yields of the seaweed K. alvarezii.