• Korean journal of applied entomology
• /
• v.40 no.4
• /
• pp.297-300
• /
• 2001

Development and reproduction of the persimmon fruit moth, Stathmopoda masinissa, were investigated under different temperatures (15, 20, 25, and 3$0^{\circ}C$). It took 96.1 days to grow from egg to adult at 2$0^{\circ}C$, 43.2 days at $25^{\circ}C$, and 34.6 days at 3$0^{\circ}C$. At 15$^{\circ}C$, all tested individuals died before pupation. The developmental threshold temperatures for egg, larva, pupa, and adult were 12.2, 13.5, 13.8, and 13.4$^{\circ}C$, respectively. The total effective temperatures for egg, larva, pupa, and egg to adult were 74.0, 331.3, 160.5, and 569.9 degree days, respectively. The hatching, pupation, and emergence rates were highest at $25^{\circ}C$. The average life span of adult prior to laying eggs and the total life span of adult were 12.6 and 29.3 days at 2$0^{\circ}C$, 3.8 and 8.6 days at $25^{\circ}C$, and 2.5 and 7.0 days at 3$0^{\circ}C$, respectively. Mean generation time in days (T) was shorter at higher temperature. Net reproductive rate per generation (R$_{o}$) was lowest at 2$0^{\circ}C$. The intrinsic rate of natural increase (r$_{m}$) was highest at $25^{\circ}C$ as 0.066.

• Korean Journal of Plant Tissue Culture
• /
• v.22 no.1
• /
• pp.47-51
• /
• 1995

This study was conducted to investigate the possibility of obtaining platelets via somatic embryogenesis as a means of in vivo mass propagation in Paeonia lactiflora Pall.. When cultured on MS medium with 0.5 mg/L 2, 4-D, filament explants formed calli. UPon transfer to basal medium the calli gave rise to somatic embryos at a frequency of 38%. Additional of 3g/L activated charcoal enhanced the frequency to 60%. Mature embryos preheated at 5$^{\circ}C$ for 2 weeks had 37% germination on medium with 0.3 mg/L GA$_3$. The germinated embryos developed to complete plantlet when cultured on medium with 2mg/L BA.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.45 no.2
• /
• pp.195-202
• /
• 2018

The purpose of this study is to analyze the cariogenicity of vitamin supplements for children by the Caries Potentiality Index (CPI), pH drop capacity, proliferation rate of Streptococcus mutans. Four vitamin supplements were selected - Noma (NM), Cenovis Kids (CK), Animal Parade (AP), and Character Vitamin (CV). CPI value decreased in the order of AP, CV, CK, and NM. Initial values of all experimental groups showed acidity below pH 7.0. Analysis of the colony forming units of Streptococcus mutans showed that NM and CV resulted a higher proliferation rate (p < 0.05) than CK and AP (p < 0.05). Bacterial activity of the control group was lower than other groups when observed with a confocal laser scanning microscope. Considering the bacterial activity and acidity of vitamin supplements, it is necessary to pay close attention when children taken the vitamin supplements for their oral health.

• Radiation Oncology Journal
• /
• v.17 no.3
• /
• pp.223-229
• /
• 1999

Purpose : The expression pattern of c-jun by ionizing radiation according to cell growth state (exponential growth vs. stationary phase) and its relationship with cell cycle redistribution were investigated. Materials and Methods : The exponential growth phase (day 4) and stationary phase (day 9) cells were determined from cell growth curve according to the elapse of days in CaSki. The cells were irradiated using 6 MV X-ray with a dose of 2 Gy at a fixed dose rate of 3 Gy/min. Northern blot analysis was peformed with total cellular RNA and cell cycle distribution was analyzed using flow cytometry according to time-course after irradiation. Results : The maximum expression of c-jun occurred 1 hour after irradiation in both exponential growth and stationary phase cells. After then c-jun expression was elevated upto 6 hours in exponential growth phase cells, but the level decreased in stationary phase cells. Movements of cells from G0-G1 to S, G2-M phase after irradiation were higher in exponential growth phase than stationary phase. Conclusion : c-jun may be involved in the regulation of cellular proliferation according to the growth states after irradiation.

• The Korean Journal of Food And Nutrition
• /
• v.13 no.5
• /
• pp.411-418
• /
• 2000

The purpose of this study was to investigate effects of chestnut inner skin tea, brown rice-green tea and Cassia lora tea on the activation of physiological functions (regional cerebral blood flow, mean arterial blood pressure, proliferation of immunocytes in vitro and in vitro, suppression of cancer cell proliferation) in mouse and rat. We used 8 weeks-old balb/c male mice, 300g ICR rats and L1210 cell lines. Regional cerebral blood flow(rCBF) and mean arterial blood pressure(BP) were measured using Leser-Doppler Flowmetry(LDF) and the proliferation of cells was measured using a colorimetric tetrazolium assay(MTT assay). The experimental results are as follows : 1. rCBF was increased by Cassia tora tea, but decreased by chestnut inner skin tea in rats. 2. BP was increased by brown rice-green tea in rats. 3. Proliferation of mouse thymocytes and splenocytes were significantly increased by chestnut inner skin tea in vitro. 4. Proliferation of mouse thymocytes was decreased by Cassia tora tea and brown rice-green tea in vitro. 5. Proliferation of mouse thymocytes was decreased by Cassia tora tea and brown rice-green tea in L1210 transplanted mice. 6. Proliferation of splenocytes was accelerated by chestnut inner skin tea in L1210 transplanted mice. 7. Proliferation of L1210 cells was inhibited by chestnut inner skin tea and Cassia tora tea in L1210 transplanted mice.

• Journal of Periodontal and Implant Science
• /
• v.24 no.3
• /
• pp.581-596
• /
• 1994

This in vitro study was undertaken to obtain optimal tetracycline concentration that aids proliferation and spreading of human periodontal ligament cells, for clinical application in root surfaces of periodontally diseased teeth. Periodontal ligament cells used in this study were obtained from explants of periodontal ligament of 1st premolar teeth which were extracted for the purpose of orthodontic treatment. The cells were cultured in Dulbecco's Modified Eagle Medium(DMEM) supplemented with 100 U/ml penicillin, $100\;{\mu}g/ml$ streptomycin and 10% FBS at $37^{\circ}C$, 100% humidity, 5% $CO_2-95%$ air. Cells were used between the third to 4th passage. After root planing of periodontally extracted teeth, the root slabs were cut with carborundum disk. In the cell proliferation experiment, experimental groups were root planing only group, immersed groups in 25, 50, 75, 100, 150mg/ml aqueous solution of Tetracycline HCl followed by a vigorous rinse in PBS. Human PDL cells at concentration of $1{\times}10^5\;cells/ml$ were seeded in each culture well which contained root slabs and incubated for 6 hours. Then, all of the root slabs were moved into new 24 culture well and incubated 24, 48 and 72 hours. The cell counting was done by inverted phase contrast microscope after trypsinization. The following results were obtained. The cell number was increased in order root planing only group, 25, 150, 50, 75, 100mg/ml of Tetracycline HCl treated group in 24, 48 and 72 hours. The maximal cell number was obtained when the root slabs were immersed in solution with 100mg/ml of Tetracycline HCl. There were statistically significant between the root planing only group and 75, 100 mg/ml of Tetracycline HCl treated group in 24 hours, between the root planing only group and 100mg/ml of Tetracycline HCl treated group in 48 hours, between the root planing only group and 50, 75, 100mg/ml of Tetracycline HCl treated group, between 25 and 100mg/ml of Tetracycline HCl treated group in 72 hours(p<0.05). In the cell spreading experiment, after 30 minutes of incubated, in the root planing only group, the cells were generally round in shape. The cell surface was mostly covered with blebs. The cells started to attach to root surface by cytoplasmic extension in 50, 100mg/ml of Tetracycline HCl treated groups, more numerous cells attached to root surface than root planing only group. Many orifices of dentinal tubule were exposed, cells showed radially spreaded cytoplasm and unspreaded central region of the cell was covered with blebs. After 6 hours of incubation, in the root planing only group, cells showed radially spreaded cytoplasm and were attached flat appearance. In 50, 100mg/ml of Tetracycline HCl treated groups, cellular margin was concaved and cytoplasm showed elongated appearance with polarity. After 24 hours of incubation, in the root planing group, cells showed characteristic polarity. In 50, 100mg/ml of Tetracycline HCl treated groups, cells showed more elongated and spindle - like appearance.

• KSBB Journal
• /
• v.7 no.2
• /
• pp.118-125
• /
• 1992

Most of cosmetics are emulsion-type products which contain the sources of nutrition, i.e., vegetable oil, mineral oil and carbohyrate etc.. These additives are usually very susceptible to the contamination by microorganisms. The purpose of this study is to obtain the data necessary not only to prevent dermalopathia occurred by microbials but also to maintain the quality. In this experiment we observed the growth of P.aeruginosa in the cosmetics with or without antiseptics so as to prevent contamination. During the contamination period, the phase became unstable and creaming phenomina was happened together with some discoloration and bad smell. The pH of cosmetic was decreased from 7.6 to 6.0 and the concentration was increased from 1.443 to 1.453 in terms of refractive index during 40 days incubation. By adding antiseptics to the cosmetics, the number of P. aeruginosa from the challenge test method were decreased from $10^8$ cell/ml to $5{\times}10^3$ cell/ml. For the antibacterial effect against P. aeruginosa, p-hydroxy benzoic acid propyl ester in phosphoric acid buffer solution showed the best result.

• Journal of Life Science
• /
• v.21 no.2
• /
• pp.300-308
• /
• 2011

Osteoporosis is a disease involving a decrease in bone mineral density and increased risk of fractures. Osteoblast and osteoclast activities are important for bone formation. The MC3T3-E1 osteoblastic cell line is a well-accepted model of osteogellsis in vitro. Hijikia fusiforme is a kind of edible brown seaweed that grows mainly in the Northwest Pacific region, including the countries of Korea, Japan and China, and it has been widely used as a medicinal and health food in Korea. In this study, by using osteoblasts, the effects of Hijikia fusiforme fractions on proliferation, alkaline phosphatase (ALP) activity, collagen synthesis and mineralization of cells were investigated. Hijikia fusiforme were subjected to fractionation by using hexane, methanol, butanol and aqueous. Proliferation of the MC3T3-E1 osteoblastic cells that were treated with Hijikia fusiforme fractions increased by approximately 120%. Regarding effects of Hijikia fusiforme fractions on ALP activity, 1 ${\mu}g$/ml butanol fraction showed the highest activity. The synthesis of collagen increased significantly in response to treatment with Hijikia fusiforme fractions, with the exception of the hexane fraction. Moreover, mineralization in the MC3T3-E1 cells that were treated with 100 ${\mu}g$/ml butanol fraction increased by 281%. Also, when 100 ${\mu}g$/ml aqueous fraction was added, mineralization increased by 240%. These results indicate that Hijikia fusiforme fractions have anabolic effect on bone through the promotion of osteoblastic differentiation, suggesting that it could be used for the treatment of common metabolic bone diseases.

• Journal of Life Science
• /
• v.21 no.12
• /
• pp.1752-1760
• /
• 2011

In this study, the effect of the Opuntiahumifusa extracts on proliferation, alkaline phosphatase (ALP) activity, collagen synthesis and ROS level of a cell was investigated using an osteoblast. Opuntiahumifusawas separated intoOpuntiahumifusapeel (OH-P), seed (OH-Se) and stem (OH-St).These were subjected to extraction by using hot water and ethanol. The proliferation of the MC3T3-E1 osteoblastic cells that were treated with OH-Se water extract were increased by approximately 120%. Regarding the effects of OH-Se on ALP activity, the $50{\mu}g/ml$ ethanol extract group showed the highest activity. The synthesis of collagen increased significantly in response to treatment with OH-Se water extract. The ROS scavenging effects of Opuntiahumifusawere investigated for involvement of oxidativedamage, cell culture and staining. Also, when OH-Se water extract $100{\mu}g/ml$ was added, the ROS level decreased by 54%. These results indicate that Opuntiahumifusa extracts have an anabolic effect on bone through the promotion of osteoblastic differentiation, suggesting that it could be used for the treatment of common metabolic bone diseases.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.08a
• /
• pp.22-22
• /
• 2020

너도개미자리는 석죽과의 다년생 초본류로 낭림산 이북으로부터 백두산까지 분포하며 고지대에 자생한다. 7~10월에 흰색의 꽃이 개화하고 종자는 달걀모양으로 표면은 밋밋하다. 본 연구는 지피용으로 사용할 너도개미자리의 분화재배 시 적정 추비, 차광 및 적심 등의 처리 정도를 알아보고자 수행하였다. 재배방법의 확립을 위하여 유용식물증식센터에서 종자를 분양받아 2019년 4월 19일에 트레이에 파종하여 충북대학교 유리온실에서 육묘한 유묘를 7월 4일에 정식하여 실험재료로 사용하였으며, 9월 24일까지 약 11주 동안 재배하였다. 공통 조건은 비닐 포트 3치(7×7cm)에 원예상토를 충진하고 200구 트레이에 셀당 1립씩 파종하여 생산된 1셀을 정식하였다. 추비, 차광, 적심 등은 무처리로 수행하였다. 추비실험은 hyponex 하이그레이드(N-P-K, 7-10-6)를 0, 500, 1000, 2000mg·L^-1의 농도로 엽면시비 하였고, 차광 실험은 0, 35, 55, 75% 차광막을 이용하였으며, 적심은 무적심과 재배 4주차의 1회적심 처리로 구분하였다. 연구의 결과, 너도개미자리는 추비처리 농도가 증가할수록 생육도 증가하는 경향이었으나, 유의성은 확인할 수 없었다. 유의적인 차이는 없었지만 2000mg·L^-1 처리구에서 지상부 및 지하부의 생체중이 가장 무거운 결과를 보였다. 차광처리 별로는 무차광 조건에서 생육이 왕성한 결과를 보였고, 차광률의 증가는 생육을 점차 억제하는 경향이었다. 개화율도 무차광에서 가장 높았으며, 다음으로 35% 차광 순이었고, 55와 75% 차광에서는 개화가 진행되지 않았다. 너도개미자리에 적심처리는 초장과 측지수의 발생을 억제하였고, 개화도 지연되는 경향을 보였다. 결론적으로 너도개미자리의 지피용 재배시 광이 충분한 에 배치하여 재배하는 것이 효과적이다. 추비는 Hyponex 하이그레이드를 2000mg·L^-1의 농도를 처리하는 것이 생육에 유리하며, 적심은 하지 않는 것이 효과적인 재배방법인 것으로 판단된다.