• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.117-117
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• 2003

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.127-127
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• 2003

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2004.10a
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• pp.105-105
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• 2004

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2004.10a
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• pp.113-113
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• 2004

• Journal of Embryo Transfer
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• v.19 no.1
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• pp.1-10
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• 2004

These studies were carried out to improve the reproductive efficiency through embryos transfer of Hanwoo IVM/IVF embryos. Following routine IVM/IVF procedure, oocytes and zygotes were cultured far 40 to 44 h in CRlaa medium with BSA. Then 2 to 8-cell embryos were removed the cumulus cell and were cultured in CRlaa medium containing 10% fatal bovine serum and 2.5 mM taurine in 5% $O_2$ and 5% $CO_2$ at 38.5$^{\circ}C$. The fresh embryos of the morulae and blastocysts cultured for 6 to 9 days in vitro or the frozen-thawed embryos were transferred into recipients. The pregnancy rates of the blastocyst produced for 6, 7, 8, and 9 days in vitro culture were 59.4, 68.2, 66.0 and 100%, respectively. In the developmental stage, pregnacy rates of early blastocysts (61.1%), blastocysts(64.7%) and expanded blastocysts(69.5%) were higher than that of morulae stage(20.0%). The pregnancy rates according to the corpus luteum grades of A, B and C in recipients were 73.6, 62.9 and 50.0%, respectively. Effects of donor-recipients synchrony of after day 2, 1 and 0, before day 1 and 2 on the pregnancy rates were 35.7, 65.5, 72.6, 67.9 and 60.0%, respectively. Pregnancy rates of the body condition score of recipients $\leq$2(71.3%) were higher than those of $\geq$3.0 score(40.0%). The pregnancy rates according to the parity of recipients when embryo was transferred to cow(70.6%) was higher than in heifer(59.1%). The pregnancy rates according to hormone treatment before embryo transfer were 69.9% in hCG + GnRH administration group and 63.0% in control group. Fresh and frozen-thawed embryos on the pregnancy rates were 70.6 and 36.4%, respectively. Pregnancy rates in single and AI+single was 90.0% and 64.8%. Pregnancy rates in twin induction was better than in single. These results indicate that pregnancy rates after transfer were affected on the embryo ages, donor-recipient synchrony, body condition score of recipients, corpus luteum status, parity and hormone treatment to recipients.

• Journal of Embryo Transfer
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• v.3 no.1
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• pp.43-47
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• 1988

공란우 89두와 수라우 94두를 사용하여 호르몬 투여에 대한 난소반응, glycerol의 평형과 제거방법에 따른 융해란자의 형태적 정상성과 동결 융해란의 이식후 임신율과 쌍태유기율을 조사하였다. 1. 호르몬처리에 대한 난소반응 :1) 과배란 처리후 배란된 난자수는 A.B.C군에서 각각 8.3, 7.8, 9.5개로서 C군이 전체 평균의 8.3개보다 증가되었다. 2) 난자의 회수율은 42.1%, 두당회수된 난자수는 3.5개였으며, 회수란자중 정상발육한 상실배와 배반포기의 난자는 63.3%, 36.7%(114/311)가 형태적 이상란자로 확인되었다. 2. 수정란의 동결 융해후 형태적 정상성 :1) 동결융해후 회수된 난자중 정상 난자의 비율은 60.4%였으며, 배반포(62.3%)가 상실배(58.3) 보다 우수하였다. 2) 1단계 glycerol의 제거시에는 동결전 glycerol의 평형을 3단계로 실시한 군(C.G)이 1단계 평형군 (A.E)보다 우수하였다. 3. 동결융해란의 이식후 쌍태유기 :1) 수정란의 이식후 임신율은 단태(대조)구에서 30.0%, 쌍태유기군인 A(AI+단태), B(편각쌍태) 및 C(쌍각쌍태)구에서 각각 55.6, 44.4, 37.5%로서 대조구보다 증가되었다. 2) 쌍태유기에 공용된 26두의 수란우에 착상된 수정란수는 17(65.4%)개로서 딘태구(30.0%) 보다 배가되었다.

• Journal of Embryo Transfer
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• v.18 no.1
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• pp.81-84
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• 2003

This study was performed to produce Hanwoo-fetal fibroblast cell clone transferred to Holstein recipient. It was coming on the parturition but didn't show parturition signs until 272 days from the embryo transfer. For the induction of normal parturition, dexamethasone (20 mg) were injected with IM on 272 day and PG $F_{2a}$ (25 mg) plus estradiol (20 mg) was injected with IM after 24hrs. Forty-eight hours after dexamethasone injection, we could find parturition signs and delivered healthy male of offspring (40 kg) about 50 hrs after it's injection. However the recipient was showed retention of afterbirth.h.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2005.10a
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• pp.95-95
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• 2005

• Journal of Embryo Transfer
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• v.5 no.2
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• pp.11-22
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• 1990

Remarkable progress has recently been made in embryo transfer technology, resulting in the birth of IVF and nuclear transfer offsprings in swine. However, further progress of the technology to (I) make a safe, effective and economic estrual-cycle synchronization compound, (2) regulate each step of sperm capacitation (3) induce monospermic fertilization, (4) in vitro grow and mature oocytes, (5) fertilize the oocytes efficently, (6) culture the oocytes to the blastocyst stage in defined media, (7) produce multiply copies of embryos with superior genetic merit, (8) preselect the sex of these superior offsprings, and (9) preserve embryos by freezing and storage in liquid nitrogen is required before this promising technology is applied routinely to swine for practical use.

• Journal of the korean veterinary medical association
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• v.39 no.4
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• pp.361-367
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• 2003

뉴질랜드 오클랜드에서 개최되는 2003년도 국제수정란이식학회 학술대회에 참석하여 축산기술연구소 종축개량부에서 시험한 '젖소 장기공태우에 PGF2, CIDR+ 및 LH 투여가 수태율에 미치는 영향'의 연구논문 포스터 발표와 IETS Data Retrieval Committee Meeting에 한국대표로 참석 및 2006년도 국제수정란이식학회를 우리나라에 유치하기 위한 홍보차 2003년 1월 10일부터 17일까지 뉴질랜드를 방문하여 수집한 자료를 기술코자 한다.