• Parasites, Hosts and Diseases
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• v.30 no.4
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• pp.237-244
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• 1992

In order to observe the infectivity, growth and development and adult morphology of Metorchis orientalis, a total of 40 chicks were experimentally infected with 100 metacercariae respectively, collected from Pseuderasbora larva. The worms of various developmental stages were recovered from chicks at 1.5, 3, 5, 7, 9, 11, 1:k and 21 days after infection, and they were prepared for morphological observations and measurements. All of the worms were found in the gallbladders of chicks, and their recovery rate was 32% in average. The growth of the body was rapid from 9 to 11 days after infection. The genital primordia appeared in 1.5 and 3-day old worms, and ovary and testes were first observed in 5-day old worms. Thereafter, genital organs gradually matured and completed up to 11 days after infection. The adult worm was leaf-like, and possessed a con- voluted tubular seminal vesicle, an ovoid ovary, a sac-like seminal receptacle, 2 lobed-testes and follicular vitellaria. Eggs were $31.9{\times}15.3{\;}\mu\textrm{m}$ in average size, ellipsoid to elliptical in shape and possessed abopercular thickenings. From the above results, it is concluded that M. orientalis grows in sigmoid pattern in chicks, and their genital organs fully matul.e between days 9 and 11. It is also confirmed that a chick is a new definitive host of M. orientalis.

• Animal Systematics, Evolution and Diversity
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• v.16 no.1
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• pp.125-131
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• 2000

This paper deals with the morphology and taxonomy of the first reported species of echinostome belonging to the genus Himasthla Dietz, 1909, found from dunlins (Calidris alpina sakhalina) in Korea. Birds were captured with mist-nets at the tidal flat of Yeochari, Kanghwa-gun, Kyunggi-do, Korea. The worms were removed from avian intestines in physiological saline, fixed, acetocarmine stained and observed. Himasthla kusasigi Yamaguti, which was found in 1939, was characterized morphologically by a long and slender body (5.27 mm in length), besetting with spines and reniform head collar (0.27 mm) in a form of a single and uninterrupted row of 31 spines. The oral sucker (0.057$\times$0.0684 mm) is smaller than the ventral sucker (0.35$\times$0.29 mm). The ventral sucker was close to the anterior extremity of the body. The worm had two elliptical testes (anterior 0.47$\times$0.30 mm, posterior 0.59$\times$0.27 mm). The testes were close to the posterior end of the body. The uterus was very long, winding and extending through at least two thirds of the body length. The ovary (0.13$\times$0.14mm) was round and small. Below the ovary, a round and small seminal receptacle was found.

• Parasites, Hosts and Diseases
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• v.27 no.1
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• pp.47-56
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• 1989

Some biological characteristics of Centrecestus armatui were studied using albino rats as its experimental host. The metacercariae were collected from Zacco Platypus by artificial digestion method. Laboratory rats(Wistar) were fed each 100 or 200 metacercariae and sacrificed on 1, 2, 3, 4, 5, 6, 8, 14 and 28 days after infection to recover worms of various ages. The average recovery rate was 10.7% from 82 rats. The rate decreased rather slowly for the first 8 days but showed a steep decrease thereafter. Of the worms, 35.5% were recovered from the duodenum and 62.5% from the jejunum. At metacercarial stage, body length was $293{\mu\textrm{m}}$ and body width $144{\mu\textrm{m}}$. At adult stage, the length and width reached $382{\mu\textrm{m}}$ and $214{\mu\textrm{m}}$ respectively at 14 days after infection. The testes and Mehlis' gland were recognized at metacercarial stage, whereas the ovarian anlage appeared on the 1st day of infection, seminal vesicle and vitellaria on the 2nd day, and seminal receptacle and uterine eggs on the 3rd day. Until 8 days after infection the genital organs developed continuously and the number of uterine eggs increased. The above results show that albino rats are one of useful experimental hosts for C. armatus and the worms can develop to adults in 3 days after infection.

• Korean journal of applied entomology
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• v.57 no.4
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• pp.329-337
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• 2018

To increase the mating rate of Bombus ignitus used as insect pollinator, we investigated the sexual maturity time of B. ignitus. In investigating ovary development such as the number of eggs per ovariole and spermatheca size, the time of sexual maturity of queen was 10 days after eclosion. In case of male, the number of sperm was 246 thousand at immediately after eclosion, and was highest as 480 thousand at 9 days, and tended to show a dramatic decline at 35 days (87 thousand). The more mating time, the less the number of sperm. In consideration of number of sperms, the time of sexual maturity of male was 3-15 days after eclosion. In the sexual maturity time of queen in mating, the queen was not mated at immediately after eclosion, and showed a decrease in 20 days. In terms of the rate of mating and oviposition, the favorable time for mating of queen was 9-20 days. On the other hand, the male showed 3.3% of mating at immediately after ecolosion, showed the highest of 43.3% at 6 days after eclosion, and tended to decrease in 25 days. The sexual maturity time for mating of male was 6-20 days. In summary, our results indicate that sexual maturity time of B. ignitus in reproductive organs and mating is most favorable in 9-12 days after eclosion for queen and 6-9 days for male.

• Journal of Sericultural and Entomological Science
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• v.15 no.2
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• pp.9-14
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• 1973

In order to find out effects of the generative power of silk worm moth which have been brought up in the high temperature accommodation at their pupa stage. For this specific study, 9 different kinds of male silk worms have been selected as specimen. All those specimen were brought up in the normal temperature at their larvae stage and after the pupation period they have been accommodated in the condition of high temperature for a certain length in accordance with the study programme. Afterwards, those mlae specimen were copulated with Suwon jam 103${\times}$104 which were all brought up in normal conditions. This study was carried out to find the copulation function as well as the ratio of unfertilized eggs(male sterility test). Results of study have been revealed as follows: 1) Although some differences were observed, male pura which have been brought up in the condition of high temperature shown the low rate of unfertilized eggs rather than those were brought up in the normal conpition. 2) In this group the eclosion(emergency) has been found to be poor rather, than those specimen brought up in normal conditions. 3) The copulation function of Moran, Daedong, J124 and C108 specimen were found to be poor than those of Suwon jam. 4) Fertility rate of Moran, Daedong, J124 and C108 was found to be around 65%. This figure is rather lower than what we normally expect. 5) Unfertilized egg ratio of Moran, Daedong and C108 were found to be around 20% if they were brought up in the condition of high temperature for one day from the time of pupation: 40% at 2 days, and 70% at 3 days duration. More than 3 days treatment has shown no progress in the unfertilized egg ratio. 6) One day's treatment for the pupa at the later stage has shown the unfertilized egg ratio of about 10%; 20% at 2 day's treatment, 35% at 3 day's treatment, 40-60% at 4 day's treatment, more than 60% at 5 day's treatment, and the 70% of fertilized egg ratio was only observed when the treatment days come to 7 days. It was understood that the unfertilized egg ratio was high at the antepupa stage rather than that of post-pupa stage. 7) According to the result of observation the sperm in copulatory pouch and seminal receptacle out of the normal female silk worm which have been copulated with the male brought up in the condition of high temperature at their moth stage. The reproduction system found in the control zone has been found to be normal and the sperm is amountful and active in motion while the sperm found in the treatment to be limited in amount and slow in motion. The observation was made within 5 hours from the copulation. 8) According to the result of observation of sperm of seminal receptacles of the female silkworm moth, and according to that observation of sperm in the seminal receptacle in female silkworm moth, the amount of sperm and mobility in the female moth brought up in high temperature were poor comparing that were brought up in normal temperature zone. Some of them are even found to be no trace of such. 9) Appearance and mosle of the copulatory organ of the male silkworm moth was found to be no anatomical change. 10) Testis of the later pupa stage which was brought up in the high temperature was found to be almost net developed to anucleate sperm and they are degenerated at stage of between maturation division and sperm abnormal stage. Mean time at control zone, the formation of anucleate sperm was already observed.

• Journal of Korean society of Dental Hygiene
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• v.10 no.4
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• pp.627-633
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• 2010

Objectives : The purpose of this study was to compare and analyse periodontal terminology of 16 dental hygiene textbooks including periodontology, dental prophylaxis for the standardization. Methods : 16 kinds of major publications were classified into the following criteria. - the standardization of translation, orthography, classification Results : 1. There are twelve cases, all told, of words with same origin used as different explanation and interpretation. Mucogingival Junction is most translated as '치은점막 경계' with high percentage of 53.8%, while Probe is translated as '치주낭 측정기' with 41.7% and Acquired Pellicle is translated as '획득피막' with 57.1%. In addition, Stippling is interpreted as '점몰' with high percentage of 70%, and Supine Position is interpreted as '수평자세' or '앙와위' with 33.3%. Yet, Pen Grasp is explained differently in all five books, whereas Modified Pen Grasp is explained as '변형 연필 잡기법' with 57.1%. The rest, Palm Thumb Grasp and Adaptation, have different interpretation in books, requiring unified interpretation of terms. 2. There are two cases of loanwords expressed according to orthography. Implant is expressed as '임플란트'(87.5%) and '임프란트'(12.5%). Scaling is expressed as '스켈링'(40.0%) and '스케일링'(60.0%). 3. There are four cases of same terms classified in a different way. Classification of 'Probing시 압력' and '치주인대섬유군' concurs only 50.0% in six books. '치석제거 시술자의 위치' is classified differently in all six books, while '정상치은 열구 깊이' concurred 45.5% in books. Conclusions : In conclusion, related academic majors and writers should provide cohesive explanation and interpretation of terms for unification of terminology in the field of periodontics as well as higher level of understanding and learning opportunities for students. Furthermore, description of all terms should be reviewed and analyzed comprehensively based on existing classification criteria in order to provide best standard explanation.

• Journal of dental hygiene science
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• v.11 no.4
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• pp.339-344
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• 2011

Matrix metalloproteinase(MMP)-9 is considered important in tissue destruction in periodontitis. The aim of this study was to investigate the influence of smoking on MMP-9 in the gingival crevicular fluid(GCF). GCF samples in upper incisors area from 332 male subjects were collected after the informed consent. The dental examination included the assessment of oral hygiene, gingival inflammation and probing pocket depth. A quantitative assessment of MMP-9 levels in GCF was performed utilizing and immunological procedure. The mean MMP-9 concentrations found in GCF of smokers(30.86 ng/ml) and quit-smokers(29.82 ng/ml) differed from non-smokers(11.33 ng/ml), adjusted by age, gingival index and Community periodontal index(p<0.001). Smoking seems to influence MMP-9 in GCF regardless of gingival inflammation and age. It means smoking can destruct the periodontal tissue for itself.

• The Korean Journal of Malacology
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• v.31 no.3
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• pp.171-178
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• 2015

The reproductive ecology of freshwater bivalve, Unio douglasiae in Lake Uiam were examined from March 2014 to February 2015. The gonads distributed in reticular connective tissue, the dioecious clams take an internal fertilization and fertilized eggs are stored in the foster-sack in the gills, then the hatched juveniles are released outside after an ovoviviparous process. The gonad index (GI) began to increase in April, reached the maximum value in July and to decrease in August. The reproductive cycle of U. douglasiae can be divided into five successive stages: multiplicative stage (November to January), growing stage (February to March), mature stage (February to May), spawning stage (April to September), recovery stage (August to December). The spawning periods were from April to September, and the main spawning occurred between April and July. The shell length at 50% group maturity was estimated to be 29.4 mm.

• Journal of Life Science
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• v.16 no.5
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• pp.729-733
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• 2006

Cryptosporidium is a waterborne pathogenic parasite which causes diarrhea. Immunomagnetic separation-immunofluorescent assay (IMS-IFA) has been a widely adopted for Cryptosporidium detection as standard method. However, this method does not provide information about viability or infectivity of Cryptosporidium. Therefore, many researchers have studied viability or infectivity analyses of Cryptosporidium with various methods such as vital staining, in vitro excystation, RT-PCR, cell culture, and mouse infection assay. In this study, two direct RT-PCR methods, cell culture RT-PCR and cell culture IFA were compared for sensitivity and other characteristics. The results showed that direct RT-PCR method with HSP70 genes had the highest sensitivity with detection up to 1 viable cell of Cryptosporidium. The infectious Cryptosporidium were detected up to 10 to 25 cells by cell culture methods in combination with RT-PCR and IFA. The infectious Cryptosporidium were apt to be quantified by cell culture IFA.

• Parasites, Hosts and Diseases
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• v.34 no.1
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• pp.1-6
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• 1996

Mccroorchis spinuLosw Ando, 1918 (Digenea: Nanophyetidael is redescribed based on adult flukes recovered from experimental mice, rats and cats which were fed with metacercariae encysted in the muscle of the crayfish, Cambnroines similis. A total of 117 adult flukes were harvested from the small intestine of the experimental animals at S-21 days post-infection. The worm recovery rate was 31-37%, not significantly different among the three kinds of animals. The metacercariae were round, 0.18 mm in average diameter, encysted with two layers of thick walls, and possessing a stylets on the oral sucker and Y- shaped exc retory bladder. The adult flukes were oval, 0.66-0.71 mm long and 0.36-0.41 mm wide, and characterized by the presence of a stylet on the oral sucker, variable location of the ovary near the mid-portion of the body, bilobed seminal vesicle, and anterolateral location of two testes. These morphological features were a little different from the original description, hence, an emended version of M. spinulosw was given. M. spinuLosw is a new intestinal trematode of animals and possibly man in Korea.