• Journal of Veterinary Clinics
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• v.11 no.2
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• pp.585-592
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• 1994

A consecutive Jiao Chio acupuncture therapy was performed for 3 days in the 45-90 days old 11 calves of which have been shown severe watery diarrhea. The discharge of the infected calves was yellowish brown in color. Two calves of these patients were infected wi pulmonary disease as well as diarrhea. Thus, Su Qi and Fei Yu acupuncture therapy was carried out additionally after dosing with antibiotics twice for The two infected calves. Blood chemical values and serum neutralizing antibody titers were checked, and total blood cell count was also carried out to know the therapeutic effect before and after(21 days) acupuncture therapeutics. The results are as follows ; 1, The diarrhea has ceased one day after begining of the acupuncture therapy in 5 calves, and the cessation of the diarrhea in remaining calves occurred in 1 calf each on 3rd and 4th day, and 2 calves on 6th day, respectively. Two calves infected with pulmonary disease as well diarrhea were cured 8 days after the begining of the therapcutics. 2. Rotaviruses wire detected in the feces of 2 calves, and bovine diarrhea viruses were detected in the 8 calves by the test for serum neutralizing antibody titers, and bovine coronaviruses were also detected in 5 calves. Four calves of the 5 bovine coronavirus infected calves were also infected with bovine diarrhea viruses. 3. Total leucocyte number, total amount of serum protein, and amount of fibringen were slightly increased, while total erythrocyte number, and erythrocyte packed cell volume were slightly decreased. These valucs were statistically not significant. Electrolytes of Na/sup +/, K/sup +/ and Cl/sup -/ were slightly decreased but these values also were not significant. These results indicate that the acupuncture therapeutics arc significantly effective to remove the viral diarrhea in the young calves.

• Korean Journal of Veterinary Research
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• v.47 no.2
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• pp.233-239
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• 2007

This study was carried out to evaluate the effects of Dochetang for the treatment of chronic diarrhea in a 4 months-old-female Korean native calf. The calf was presented to the Wow Animal Clinic, Iksan with the history of chronic diarrhea for several weeks. The feces test did not reveal the presence of the parasites or microbes causing diarrhea in calf. The blood test was also negative to the virus that causes of diarrhea in calf. Adminstration of parenteral antibiotics resulted in improvement of the condition temporarily but diarrhea was recurred again after 2-3 weeks. Then the calf was treated with Dochetang administered orally once a day in an empty stomach for 15 days. Feces was significantly reduced in moisture on 7 days after initial treatment. On 9 days after initial treatment, the calf had normal appetite and defecation in physiological conditions. Blood samples were collected before administration and on 1, 2 and 3 weeks after initial administration of Dochetang for hematology and biochemistry. A significantly differences were observed in the white blood cell (WBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), albumin (ALB), glutamic pyrubic transaminase (GPT), inorganic phosphorus (IP) and magnesium (Mg), while no significant differences were seen in the red blood cell (RBC), hemoglobin (Hb), hematocrit (HCT), platelet (PLT), glucose (Glu), total protein (T-pro), glutamic oxaloacetic transaminase (GOT), blood urea nitrogen (BUN) and creatine (CRE). This study suggests that Dochetang administration can be a successful alternative therapeutic agent in instead of antibiotics for the treatment of chronic diarrhea in calves.

• Korean Journal of Veterinary Research
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• v.46 no.2
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• pp.135-142
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• 2006

Shiga toxin (stx) producing Escherichia coli (STEC) causes various clinical signs in animal and human. In this study, 255 fecal samples from calves showing diarrhea were collected from cattle farms in Chonnam province during the period from January 2005 to July 2005. Twenty six STEC (10%) were isolated from 255 fecal samples by PCR. The isolates displayed three different stx combinations (stx1 [69%], stx1 and stx2 [15%], and stx2 [38%]). The isolates were further studied for virulence associated genes and antimicrobial resistance to define the virulence properties. Intimin (eaeA), enterohemolysin (hlyA), and lipopolysaccharide (rfbE) virulence genes were detected in 6 (23%), 7 (26%), and 1 (3.8%) of the isolates, respectively, by PCR. One isolate possessing rfbE gene was typed as E. coli 0157 : H7 by agglutination test with O and H antisera. All 26 isolates showed susceptibility to amikacin (100%) and the majority of isolates showed high susceptibility to gentamicin (88.5%) and chloramphenicol (73.1%). But all isolates were resistant to penicillin. These results may provide the basic knowledge to establish strategies for the treatment and prevention of enteric disease in calves.

• Korean Journal of Veterinary Service
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• v.44 no.1
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• pp.1-9
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• 2021

This study was performed to investigate the causative agents of diarrhea disease from Korean native calves in Jeonbuk western area. A total of 273 samples were collected in 2019. Analysis of causes of diarrhea shows that BVDV 8.1% (22/273), BRVA 17.6% (48/273), BCV 7.7% (21/273), E. coli 13.6% (37/273), Clostridium 5.1% (14/273), Parasite 1.1% (3/273) and other 46.9% (128/273) were detected. For major virus diseases (according to dietary changes), less 1 month 9.1.% (2/22), 1~3 months 18.2% (4/22), 4~6 months 18.2% (4/22), 7~13 months 36.4% (8/22) and over 13 months 18.2% (4/22) in BVDV, less 1 month 10.4.% (5/48), 1~3 months 60.4% (29/48), 4~6 months 4.2% (2/48), 7~13 months 4.2% (2/48) and over 13 months 20.8% (10/48) in BRVA, and less 1 month 23.8% (5/21), 1~3 months 23.8% (5/21), 4~6 months 23.8% (5/21), 7~13 months 19.0% (4/21) and over 13 months 9.5% (2/21) in BCV could see that the infection of the pathogen changed due to dietary changes. In particular, the infection rate of the BVDV is the highest at 36.4% (8/22) between 7~13 months, which requires the need to build research on the PI's that could have the effect of spreading the disease, different from those of BCV and BRVA. These results are likely to contribute to improving the productivity of raising at Korean native cattle.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.537-541
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• 2007

The purpose of this study is to examine and compare the concentrations of blood retinol and $\alpha$-tocopherol in normal and diarrhea calves. The subjects of the experiments are from three groups. Each group consists of 5 calves(diarrhea, treatment and control group). The CBC, serum chemistry, serum retinol and a-tocopherol concentrations were estimated in each group. Blood retinol concentration was $13.3{\pm}7.0{\mu}g/100ml$ in diarrhea group, $31.5{\pm}6.9{\mu}g/100ml$ in treatment group and $28.1{\pm}11.8{\mu}g/100ml$ in the control group. The blood concentration in diarrhea group was significantly lower than that of the control group in the case of retinol(p<0.05). However, there were no significance between the treatment poop and the control group. The $\alpha$-tocopherol concentration in blood was $266.0{\pm}127.6{\mu}g/100ml$ in diarrhea group, $432.2{\pm}172.7{\mu}g/100ml$ in the treated group and $579.3{\pm}145.8{\mu}g/100ml$ in the control group. In the case of $\alpha$-tocopherol, the laboratory group were significantly lower than the control group(p<0.05), except for the treatment group. As in retinol concentration there were no significance between treatment group and the control group. In the test of CBC, PCV was significantly lower in the group with diarrhea than the control group(p<0.05). Fibrinogen concentrations in diarrhea calves were significantly higher than the treatment and control group. In conclusion, the blood retinol and $\alpha$-tocopherol concentration in diarrhea calves are lower than normal calves. Medication of retinol and $\alpha$-tocopherol on calves with diarrhea is recommended.

• Korean Journal of Veterinary Service
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• v.35 no.4
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• pp.339-342
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• 2012

The objective of this study was to determine the infection patterns of etiological agents causing calf diarrhea in the Gyeongnam province, Korea. In this study, from January 2011 to December 2011, feces and necropsy specimens from 249 calves diagnosed with diarrhea (<7 months old) were examined by reverse transcriptase-polymerase chain reaction assay and bacteria & coccidium isolation for detection pathogenic organism. The results of this study showed that 78 cases (31.3%) in spring, 71 cases (28.5) in summer, 62 cases (24.9%) in fall and 38 cases (15.3%) in winter were diagnosed with calf diarrhea, respectively. Calf diarrhea-causing pathogens were diagnosed as bacteria 113 (45.4%), viruses 97 (39.0%), coccidium 1 (0.4%), unknown cases 13 (5.2%), and mixed infections 25 (10.0%). We isolated three virus types from fecal samples (97), which were classified as BVD 64 (66.0%), BRV 21 (21.6%), and BCV 12 (12.4%). Moreover, co-infected pathogens were 25 cases, consisting with BVD & BRV 11 (44%), BVD & BCV& BRV 7 (28.0%), E. coli & BCV 3 (12%), and BVD & IBR 1 (4.0%). In summary, we demonstrated that the enteropathogens of bacteria, viruses, and parasite were detected in samples from cattle with diarrhea, principally in young calves less than 7 months of age. Future studies of infectious diarrhea in cattle should include assays for this etiologic agent.

• Korean Journal of Veterinary Service
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• v.40 no.1
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• pp.61-66
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• 2017

Calf diarrhea is a disease experienced by almost all of calves after birth and is one of the representative causes of damage to farmers due to mass mortality and of economic losses to them by inhibiting normal growth. In this study, we conducted quick detection of etiologic agents of diarrhea by using a rapid diagnostic kit to multiply diagnose antigens of five etiologic agents of calf diarrhea (rotavirus, coronavirus, Escherichia coli, Cryptosporidium, Giardia) in Hanwoo (Korean native cattle) calves. When the positive antigen proportion of the calf diarrheal feces for each farm was analyzed, rotavirus, coronavirus, Escherichia coli, Cryptosporidium, and Giardia showed antigen positive rates of 0~67%, 0~20%, 0~60%, 0~20%, and 0~67%, respectively. With regard to the antigen positive rate by age in days after birth, 1-week-old calves showed the antigen positive rate of 20% in rotavirus and 20% in Giardia, and 2-week-old calves showed that of 50% in rotavirus. In addition, 4-week-old calves showed the antigen positive rate of 10% in rotavirus, 10% in coronavirus, 10% in Escherichia coli, and 30% in Giardia, and 8-week-old calves showed the antigen positive rate of 17% in coronavirus, 50% in Escherichia coli, 17% in Cryptosporidium, and 33% in Giardia. Based on the results of this study, the etiologic agents of diarrhea in Hanwoo calves for each farm are widely distributed. Although younger than 2-week-old calves were strongly positive for rotavirus, older than 4-week-old calves were highly positive for Giardia and Escherichia coli. In conclusion, we considered that a rapid diagnostic kit is an effective method for quick detection of etiologic agents and would be helpful for cattle farmers and veterinarians to select appropriate therapeutic method.

• Korean Journal of Veterinary Service
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• v.30 no.1
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• pp.133-144
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• 2007

This study was conducted to determine the effective environmental control measures in preventing calf diarrheal disease in farms. It was found that the high numbers of calves in a cow house was directly related with the high incidence rate of diarrheal diseases. When the number of calves was increased in a cow house, it was difficult to reduce the diarrheal diseases of calves. It was revealed that the incidence rates of calf diarrheal disease were dramatically reduced when the delivery house which faced south. The periodical changes of bedding materials seemed beneficial good to control the possible resident infectious agents, including pathogenic viruses and bacteria. The control of the delivery time was important for reduction of calf diarrhea. It was revealed that September and October were the best time as the delivery period. In order to use old cow house as a delivery house, it should be sterilized by the disinfectant and the flame. When the diarrheal feces were removed as soon as possible, the transmission of diarrheal diseases among calves In the same house was reduced. When the diarrheal feces were not disposed, the incidence rate of diarrhea reached up to 50%. When the morbid calves were not quarantined, the incidence rate of diarrhea was 100%. In contrast, when the diseased calves were quarantined from other calves, the incidence rate of diarrhea was reduced, remarkably. The mean recovery time from the diarrhea of the treated calves in the southern cow house was much faster than that in the north cow house. The treatment of calves in a clean and well-ventilated cow house with dry bedding was more effective than that in a cold, wet, and dark house. It could be postulated that the most important environmental factor in the reduction of diarrheal diseases of calves is the blocking of the entry of the infectious agents. The complete sanitation and control of the delivery house in the using farm is more important than building a new cow house, for prevention of calf diarrheal diseases.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.76-81
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• 2006

창녕군 증산리 지역은 신규 취수원 확보 일환으로 강변여과 방식 취수 가능성이 한국수자원공사에 의해서 평가되고 있는 지역이다. 강변여과 방식 취수의 타당성 평가를 위해 현장 지질조사, 시추조사, 전기 비저항 탐사 및 고해상도 탄성파 탐사를 수행하였다. 또한 대상 연구지역에서의 개략적 취수 가능량 산정을 위해 모델링 평가가 수행되었으며, 개별 정호에 대한 양수량 산정을 위해 시험 정호를 설치하였다. 현장 시추 조사와 시료에 대한 입도 분석 결과, 연구 지역의 충적층 두께는 35m 전후이며, 주 대수층 구간은 지표하 $25{\sim}35m$인 것으로 추정되었다. 또한 주 대수층 구간의 수리전도도는 $10^{-2}cm/sec$ 이상으로 주로 모래섞인 자갈층으로 구성되어있는 것으로 평가되었다. 또한 전기비저항 조사 결과는 부분적으로 매우 낮은 비저항 분포 지층을 보여주고 있으며, 이는 주로 실트 및 점토로 된 지층이 부분적으로 퇴적되어 있음을 지시하며, 고해상도 탄성파 탐사 결과는 전반적으로 지하수위는 지표하부 5m 전후에 분포하고, 충적층의 하부 경계는 35 내지 45m인 것으로 해석되었다. 지하수 모델링을 통해, 취수 목표량인 180,000톤/일은 주대수층까지의 지하수위 강하 없이 확보 가능할 것으로 평가되었다. 또한 개별 정호의 산출 특성을 평가하기 위해 시험 정호를 설치하여, 실제 2,700톤/일 예비 양수 시험을 수행하였다. 예비 양수 시험 결과, 양수정에서의 수위 강하는 개략 10m, 양수정관측정에서는 약 0.3m의 수위강하만이 관찰되었으며, 양수 영향권이 수 십 m를 넘지 않을 것으로 판단되었다.서의 S97과 JBR의 세포감염 억제율은 3.85%와 3.63%로 나타났다. $textsc{k}$-casein, CU는 로타바이러스 S97과 JBR에 대해 농도 2000UM에서 97%이상의 억제효과를 나타냈으며, sialic acid는 억제효과가 거의 없었다. K-casein, GMP는 송아지뿐만 아니라 유아의 로타바이러스에 의한 설사를 억제할 수 있을 것으로 기대된다.을 향상하기 위해서는 이러한 부위에 대한 미생물 오염을 낮출 수 있는 세심한 현장 품질관리가 필요하다.en and adolescents, analysed by country, age group and gender. The paper discusses the places young consumers can turn to in trying to fulfil their growing consumer needs. It also examines how much money is at their disposal. It then concludes by considering the influence of "financial socialization" on how young people deal with money.nsumption visions based on the various perspectives, consumers are influenced in the apparel buying decision-making. Many subjects reported experiencing positive affect when imagining positive outcomes of produc

• Korean Journal of Veterinary Service
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• v.44 no.4
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• pp.271-281
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• 2021

Pathogenic Escherichia coli (E. coli) is one among the most important agents of diarrhea in calves. From January to December 2021, 108 isolates from feces of calves with diarrhea were investigated for enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC), and enteroinvasive E. coli (EIEC) using real-time PCR. In addition, the genes for F5, F17 and F41 fimbriae were detected by PCR. The most frequently isolated pathotypes were EPEC/STEC (29 isolates), and ETEC/EPEC/STEC (29 isolates). ETEC/EPEC, and ETEC/STEC were also found in 10 isolates. EPEC, STEC, and ETEC were detected in 13, 11, and 6 respectively. EAEC, and EIEC was not detected. Antimicrobial resistance test was carried out by agar disc diffusion method with 14 antimicrobials. Among 108 pathogenic E. coli isolates, 107 isolates were resistant to at least one of 14 antibiotics used in this study, 99 (91.7%) were resistant to two or more antimicrobials, and a single remarkable isolate was resistant to 14 antimicrobials. The isolates were primarily resistant to penicillins, streptomycin, tetracycline, ceftiofur, Trimethoprim/sulfamethoxazole, Kanamycin, and Ciprofloxacin. The high rate of resistance in pathogenic E. coli, sometimes to multiple drugs, may complicate future options for treating human infections. These results may bu used for diagnosis and therpeitic purposes in calves with diarrhea.