• Journal of Oral Medicine and Pain
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• v.34 no.3
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• pp.237-245
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• 2009

The purpose of this study was to investigate the clinical efficacy of latex cover developed for dental handpiece on contamination of microorganisms during dental treatment and to determine whether it can be an alternative to conventional sterilization such as autoclaving. E. fecaelis was used as a experimental microorganism instead of oral flora. Experimental bowl with 2 cm of rectangular cavity was fabricated for handpiece operating instead of oral cavity. Latex covers ($Orokeeper^{(R)}$, Orobiotech Co., Korea) and several handpieces were used after sterilization by autoclave. Four experiments were performed to evaluate bacterial contamination related with (1) various parts of dental handpiece, (2) swabbing time with alcohol sponge, (3) postoperative air-water spraying time and (4) consecutive use of latex covers without autoclaving. The results show that face of handpiece uncovered with latex cover was severely contaminated than the covered area and that most bacteria were removed by swabbing face and head area of dental hand-piece and by air-water spraying more than 15 seconds nearly up to the level of sterilization. Conclusively it can be suggested that use of latex cover for handpiece during dental procedure, swabbing with alcohol sponge is air-water spraying for more than 15 seconds after use of dental handpiece should be very useful and practical for prevention of cross infection and should be an alternative method for the sterilization of dental handpiece under some difficult situations not being able to sterilize a handpiece with autoclave.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.5-5
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• 2010

제주지역에 분포하는 특산식물의 현황을 알아보기 위해 지난 2007년부터 2010년까지 현지조사를 실시하여 44과 75속 63종 17변종 13품종으로 총 93분류군에 대한 자생지 환경, 개체군 동태, 생육특성 및 위협요인 등에 대한 자료를 구축하였다. 이중 세계적으로 제주지역에만 분포하는 제주특산식물은 긴다람쥐꼬리(Huperzi integrifolium) 등 27과 46속 36종 12변종 8품종으로 총 56분류군으로 분석되었으며, 제주도를 포함하여 우리나라에만 분포하는 한국특산식물은 구상나무(Abies koreana) 등 20과 28속 24종 4변종 4품종으로 총 32분류군인 것으로 파악되었다. 그리고 조사대상 특산식물 중 바위좀고사리(Asplenium sarelii var. anogrammoides) 등 5분류군은 분류학적 검토와 함께 특산식물여부에 대한 검토가 필요한 것으로 판단되었다. 그리고 개나리(Forsythia koreana)는 조경용으로 식재된 종이므로 제주지역에 자생지가 확인된 특산식물은 39과 70속 59종 16변종 12품종으로 총 87분류군으로 볼 수 있다. 제주지역의 특산식물은 해안지대에서 확인된 갯겨이삭(Puccinellia coreensis)부터 한라산 정상에서 자라는 한라솜다리(Leontopodium hallaisanense)까지 제주도 전역 분포하고 있으나 종에 따라 서로 다른 분포특성을 보였다. 특히, 한라산 해발 1,400m 이상 아고산대에 집중 분포하는 특성을 보였다. 특산식물의 자생지내 개체군의 동태를 보면 대부분의 식물이 개체수 및 분포범위가 한정되어 있는 것으로 나타났다. 더욱이 지리산오갈피(Eleutherococcus divaricatus var. chiisanensis)는 2개체만이 확인되어 종보전이 시급한 것으로 조사되었고, 한라솜다리, 모데미풀(Megaleranthis saniculifolia), 한라각시둥굴레(Polygonatum humile var. humillimum) 등 상당수의 식물 종이 수십 개체 이내로 매우 적은 개체수를 유지하고 있었다. 제주지역에 분포하는 특산식물은 인위적 또는 자연적 요인으로 인해 많은 자생지 면적감소나 개체수 감소 등의 위협요인을 갖고 있는 것으로 나타났다. 특히, 중산간 이하에 자라는 특산식물은 접근성 등이 용이하여 도채 등의 위험성이 높은 것으로 판단된다. 그리고 한라산국립공원 내에 분포하는 특산식물은 인위적 훼손 위험성은 상대적으로 낮은 편이나 자연적 요인 즉, 식생변화 등으로 인해 자생지 및 개체수 감소가 이루어질 것으로 보여 체계적인 종 보존전략이 마련되어야 할 것으로 판단된다. 한편, 제주지역 특산식물 중 자생지가 확인된 식물 종을 대상으로 근연종 분석 등을 통해 유용성을 분석하면 식물체 일부가 약용으로 가능한 식물 종이 개족도리(Asarum maculatum) 등 전체의 67.1%를 차지하였고, 관상용으로 활용가치가 높은 식물 종은 구상나무(Abies koreana) 등 전체의 60.2%인 것으로 분석되었으나 대부분의 종이 희소성의 가치가 있어 모든 특산식물이 관상용으로 이용가치가 있는 것으로 판단되었다. 제주지역에서 확인된 특산식물 중 전체적으로 90.9%가 유용성이 있는 것으로 나타나고 있으며 아직까지 유용성에 대한 정보가 미흡한 종도 자원적인 연구접근에 따라 다양한 자원으로 활용가치가 있는 것으로 판단된다.

• Journal of Conservation Science
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• v.31 no.1
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• pp.1-11
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• 2015

Seated Amitabha Triad at Muwisa Temple (Treasure No. 1312) had been known as wooden Buddha statue, but a precise safety inspection revealed that the statue is terracotta Buddha statue made with clay. The clay layer of Amitabha Triad was conserved due to its severe damage. In this study, experiments were conducted to produce the most appropriate filler for the conservation treatment of the seated Amitabha Triad. Mixed clay samples with various ratios were produced and surface hardening state, crack, color change, and shrinkage of the samples during dry process were measured. Loess, fine sand powder, and cotton were used to produce the mixed clay for the filler with six different ratios and then 12 different concentration glues made with glutinous rice glue, Pachymeniopsis Elliptica glue, and animal glue were added as adhesives. Total 72 types of samples were prepared and comparative study was conducted. As a result, when the mixed clay contains 2.5% cotton compared to the weight per cent of loess and fine sand powder and also loess and fine sand in the mixed clay have a 15:1 ratio, the mixed clay had the lowest shrinkage. Animal glue is considered as an appropriate glue since it had small color change, low physical property change and shringkage. Therefore, mixed clay (loess:fine sand=15:1) mixed with 15ml animal glue is likely to be a suitable filler for conservation treatment of the seated amitabha triad at the Muwisa Temple.

• Applied Microscopy
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• v.29 no.2
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• pp.149-162
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• 1999

For investigation of the activated carbon on the mouse kidney treated with Pb, the activated carbon (40 mg/kg) and Pb (30 mg/kg) were treated orally for three and six weeks, respectivelly, and observed by the electron microscope. On the glomerulus of the group with only Pb, the basal membrane thicked, projected, and the foot processes fused. On the proximal convoluted tubules, the number of microvilli were decreased and the number of vacuoles and lysosome increased on the cytoplasm. The mitochondria and endoplasmic reticulum were extended and ribosomes dropped from the ER. On the giomerulus of the group with Pb-activated carbon, the basal membrane and the foot processes were merely changed. On the proximal convoluted tubules, the shapes and number of microvilli were not changed and the number of vacuoles, microbodies, and lysosomes decreased. The shapes of mitochondria and endoplasmic reticulum observed almost similar with control group. Th at is, elongated mitochondria and attached ribosomes to endoplasmic reticulum. As result, the activated carbon has positive effect on reducing toxicity of lead in the mouse kidney in the view of electron microscope.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.635-644
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• 2018

This descriptive study examined the practical nursing guidelines for the prevention of pressure injuries (PI) in operating rooms and their application. Method: Four general hospitals and three specialized hospitals located in Seoul, Incheon, and Gyeonggido province were selected through an online randomization program and nurses at these hospitals who participated in operations and had at least six months work experience were surveyed. Data were collected from January to April 2018 through a survey distributed to 150 nurses, 129 of whom returned the survey (86% recovery rate). The results showed that 43 nurses provided PI prevention care for patients in a lateral position (33.3%), 37 for patients in a supine position (28.7%), and 36 for patients in a prone position (27.9%). Gel was most widely used as the supporting surface material (102 nurses; 79.1%), followed by sponge (62 nurses; 48.1%), and cotton (47 nurses; 36.4%). Skin was often inspected twice, before and after the use of a supporting surface (90nurses; 69.8%), but no designated tool was used to determine the risk of PI developing (76 nurses; 58.9%). Additionally, the patient's position during surgery (83 nurses; 64.3%) and the length of the operation (i.e., more than two hours, in this case) (49 nurses; 38.0%) were determining factors for whether to use a supporting surface. The operating room nurses used the gel, sponge, and cotton as the supporting surfaces for the prevention of PI and confirmed that the cutoff operation time for the use of a supporting surface was more than 2 hours.

• Journal of Life Science
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• v.32 no.6
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• pp.463-467
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• 2022

As a result of applying the COG (Cluster of Orthologous Groups of Protein) algorithm to 1,309 species to confirm the conserved genes of prokaryotes, ribosomal protein S11 (COG0100) was identified. The numbers of conservative genes were 2, 5, 5, and 6 in 1,308, 1,307, 1,306, and 1,305 species, respectively. Twenty-nine genes were conserved in over 1,302 species, and they encoded 23 ribosomal proteins, 3 tRNA synthetases, 2 translation factors, and 1 RNA polymerase subunit. Most of them were related to protein production, suggesting the importance of protein expression in prokaryotes. The highest conservative COG was COG0048 (ribosomal protein S12) among the 29 COGs. The 29 conserved genes usually have one protein for each prokaryote. COG0090 (ribosomal protein L2) had not only the lowest conservation value but also the largest standard deviation of phylogenetic distance value. As COG0090 is not only a member of the ribosome, but also a regulator of replication and transcription, it could be inferred that prokaryotes have large variations in COG0090 to survive in various environments. This study could provide data necessary for basic science, tumor control, and development of antibacterial agents.

• Korean Journal of Veterinary Research
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• v.52 no.1
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• pp.25-31
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• 2012

Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.

• Journal of Pharmaceutical Investigation
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• v.22 no.2
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• pp.115-124
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• 1992

The characteristics and stabilities of phosphatidylcholine liposomes covalently coupled with immunoglobulin fragments prepared by the REV method were investigated by the dynamic light scattering, absorbance and calcein release. Using a sulfhydryl-reactive phospholipid derivative of N-[4$({\rho}-maleimido-phenyl)$ butyl] phosphatidylethanolamine (MPB-PE), Fab' antibody fragments were covalently combined with preformed large unilamellar vesicles (LUV), Coupling ratio was $250\;{\mu}g$ of $Fab'/{\mu}mol$ of phospholipid in vesicles, From dynamic light scattering, it was found that the size of the vesicles increases as the ratio of cholesterol to lipid increases, but that apparently, the size of liposomes was not sensitive to the existence of Fab' fragments. Regardless of inserting Fab' fragments, the absorbance of liposomes decreased as the amounts of bile salt (BS) added. At very low BS concentrations, BS/lipid aggregates would be formed in the outer vesicles monolayer, while, at the high BS concentrations, mixed micelles would be preferred. The vesicles incorporated with Fab' fragments, however, are more resistant to the bile salts than the MPB-PE vesicle are. The absorbance of vacant liposomes and calcein release resulted in that the Fab' vesicles and MPB-PE vesicles by the REV method are very stable, but that those by the sonication method sufferred the significant change of turbidities.

• Journal of the Korean Society of Costume
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• v.52 no.8
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• pp.109-122
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• 2002

This study is to understand cotton Tyeollik construction of the middle Chosun Period through the reconstruction of tyeoilik which was excavated from lady kim's tomb (from sooncheon). The form of excavated Tyeollik classified into two types by the location of waistline. The structure of excavated tyeollik classified into three types by the construction(one-layer, two-layer, cotton/quilt) The form type change according to the period. The change of the form type has the close relation structure type in the tyeollik. Cotton-tyeollik belongs to the form of the front time. The construction of cotton tyeollik is similar with the one-layer Tyeollik Exception the construction of git(collar) and the size and direction of the fold. The surface pattern is composed of two-pieces but the liner is one-piece in the Git(collar). and In The size of fold surface is o.2-o.3cm, and liner is 0.5-0.8cm. The direction of fold in the liner is the contrast with the surface.

• Journal of Pharmaceutical Investigation
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• v.35 no.1
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• pp.25-31
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• 2005

Depot system for local drug delivery using chitosan hydrogel has been developed to enhance the therapeutic efficacy and to prevent the severe side effect in whole body. Thus, we have prepared an injectable chitosan hydrogel containing liposomes to treat cancers clinically. Anionic liposomes incorporated to improve sustained release efficiency within chitosan hydrogel. The chitosan solution containing liposomes was designed to form a hydrogel complex at body temperature. The released behavior of doxorubicin from liposomes in chitosan hydrogel showed sustained-release caused by diffusion of doxorubicin from temperature responsive liposome into chitosan hydrogel. The chitosan hydorgel containing liposomes enhanced the therapeutic potency for the solid tumor in vivo system. Our results indicate that the liposomes in chitosan hydrogel represent a depot system for local drug delivery.