• Proceedings of the Korean Society of Grassland Science Conference
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• 2002.09a
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• pp.26-27
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• 2002

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.2
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• pp.111-115
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• 1982

The trials were carried out for improvement of early maturing wheat varieties, desease resistant and good quality through transfering good characters of barley to the wheat, during 1980-'81. For these purpose, Chinese Spring, a wheat variety was used as male and Manker 16 a barley variety, as female parents. EACA($\varepsilon$-Amino Caprolic Acid) known as an inhibitor of blood coagulation was injected to all barley from 14 days until heading with 1$m\ell$ per culm at the level of 500ppM. $GA_3$ solution which accelerates pollen activity was also used with 75ppM concentration after pollination for two days including check to determine the single effect of EACA. Two different methods of the cross were used. One was bud-pollination, another was post-pollination. The result indicated that the post-pollination was better than bud-pollination in terms of pollination rate, even though formation rate of normal embryo was seemingly adversed. The new plant derived from barley-wheat cross was similar in phenotype to the wheat.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.257-262
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• 1997

Intergeneric crosses over 34 cross combinations between genus Brassica and Raphanus were made. Ovules taken out of crossed were cultured on MS media supplemented with 1.0 mg/L GA and BA. Germination of embryo from ovule culture was not influenced by BA and GA in medium but by parental characters in its cross combination. Use of Raphanus sativus cv. Daibyosobudore and Jungkukcheongpi showed low embryo germination when they were used as male part. Cross combination with Brassica juncea as male parent showed slightly increased germination compared to other cross. These results indicated that embryo germination in ovule culture was not much influenced by casein hydrolysate, malt extract, BA, kinetin and glutamine in the medium, but parents in combination were key factor for increasing embryo germination.

• Korean Journal of Plant Tissue Culture
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• v.22 no.5
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• pp.261-266
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• 1995

This study was carried out to develop new varieties which are dwarf and tolerant to winter cold in the Aurantioideae by intergeneric crossing. to do that, the reciprocal crosses of Hwanggeumyooza and trifoliate orange, yooza and trifoliate orange were done and in vitro immature ovule culture of their hybrid was carried out .The callus formation from immature ovule was good in order of Hwanggeumyooza, Hwanggeumyooza $\times$ tifoliate orange, yooza, and trifoliate orange and best at 1 to 3 mg/L NAA+0.5mg/L zeatin on MT medium. In vitro germination percentage of 20week old hybrid of Hwanggeumyooza $\times$ tifoliate orange and trifoliate orange $\times$ Hwanggeumyooza were 41.3% and 37.7, respectively. The phenotype of hybrid (95%) of Hwanggeumyooza $\times$ trifoliate orange and that (100%) of trifoliate orange $\times$ Hwanggeumyooza were similar to that of trifoliate orange. After Hwanggeumyooza was pollinated by pollens of trifoliate orange, the pollen tubes grew on stigma after 3h of pollination and entered into micropyle after about 24~28 h. One gamete in pollen was fused with polar nuclei after 2 days and other one fused with egg nucleus at 3days after pollination. The fruit set percentage by intergeneric crossing was 14.0% in Hwanggeumyooza $\times$ trtfoliate orange and 17.5% in trifoliate orange $\times$ Hwanggeumyooza. The fruit set percentages of Hwanggeumyooza. and trifoliate orange were 34.2% and 39.5% by artificial self-fertilization, 34.2% and 39.5% by artificial cross fertilization, 3.1% and 1.4% by parthenocarpy and 13.0% and 3.0% by natural fertilization, respectively. The somatic and gametic chromosome numbers of Hwanggeumyooza, yooza, and trifoliate orange were 2n=18 and n=9.

• Korean Journal of Ichthyology
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• v.22 no.4
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• pp.225-229
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• 2010

A intergeneric hybrid between Rhodeus uyekii and Acheilognathus signifer was collected in the Jojongcheon Bukhan River. The hybrid showed intermediate characteristics of two parental types in some key characters, such as color pattern of body, pigmentation of dorsal, anal, and caudal fins, and body spot and stripe. In addition, the hybrid had a fairly large number of lateral line scales, while it lacked a barbel, which is known as a key intergeneric character. The probable occurrence of a natural hybrid is supported by a viable $F_1$ hybrid produced with the parental species.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.497-507
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• 2015

It is necessary to evaluate the resistance to disease among genetic resources for development of disease resistant grapes. This study was conducted to screen the resistance to crown gall in muscadine and Florida hybrid bunch grapes by pathogen inoculation. In order to compare the responses to infection with different pathogen strains, muscadine and Florida hybrid grapes were inoculated with 3 strains of Agrobacterium vitis. Although there were different levels crown gall formation among grape cultivars, there little variation in response to inoculated strains. Among 29 muscadine cultivars tested by inoculation of A. vitis 'C4612', most of them were shown to be susceptible, and 'Gold Isle' and 'Africa Queen' were highly susceptible, and two cultivars, 'Welder' and 'Jumbo' were found to be resistant to crown gall disease. Among Florida hybrid grapes, 'Daytona', 'Stover', and 'Swanee' were susceptible and 'Blanc du Bois' was moderately susceptible to crown gall. Because muscadine grapes have been actively utilized as useful genetic resources for development of new grape varieties by intersub-genus cross, this result from the screening of resistance among muscadine grapes can provide valuable information in breeding programs of grape resistant to crown gall.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.273-278
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• 2002

Several regenerated plants were obtained from anther culture of the hybrid lines of Italian ryegrass (Lolium multiflorum Lam.) X Tall fescue (Festuca arundinacea Schreb.). When the anthers of the hybrid plants were incubated for 20 days on callus induction medium, MS medium containing 30 g/$\ell$ of sucrose, 2 mg/$\ell$ of NAA and 1 mg/$\ell$ of kinetin, their calli were induced. The mean ratio of callus induction was 11.6 percent, and the mean of callus weight was 9.1 mg/callus/anther. When the calli of the hybrid plants were incubated for 50 days on plant regeneration medium, MS medium containing 30 g/$\ell$ of sucrose, 1 mg/$\ell$ of NAA and 2 mg/$\ell$ of kinetin, the hybrid plants were regenerated. The mean ratio of plant regeneration was 27.1 percent and line Hyb-1 showed highest regenerabillity with the frequency of 30.2 percent.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.2
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• pp.91-96
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• 2006

This experiment was carried out in order to examine the winterhardiness, heading date, fresh and dry matter yeild of Festulolium braunii, a hybrid of Festuca pratensis and Lolium multiflum, which is newly introduced in Korea. This experiment was performed at National Livestock Research Institute in Suwon and Namwon from September 2004 to July 2005. The results are as follows. 'Fleurial', 'Boxer', and 'Perun' showed stronger winterhardiness than 'Hwasan 101', which is one of the strongest in the winterhardiness hybrids. Therefore, we do not see there is any problem in winterhardiness when these hybrids are cultivated in Korea. The hybrids are turned out to be mid and late maturing hybrid. The average heading dates of the three hybrids were May 25 in Namwon and May 27 in Suwon. These dates are lower($1{\sim}3$ days) than that of Hwasan 101. They showed $91{\sim}96cm$ in leaf length at 1st cutting times which are comparatively long. This experiment also disclosed that their lodging and regrowth are strong and excellent. We also found out that there are significant difference between regions and hybrids in the total fresh yield per ha. The average fresh yield of Suwon was 83,222 kg/ha and that of Namwon was 69,139 kg/ha. We could not see any difference in the dry matter yield affected by region, however, there were significant difference in hybrids(p<0.05). The crude protein contents of the three hybrids indicated very high figures. the crude protein cotents of Perun, Fleurial and Boxer are 18.0% 17.4% and 16.7% respectively. However the ADF (Acid Detergent Fiber) shows $28.1{\sim}29.2%$ which is less contents than 33.2% of Hwasan 101. We found out from this experiment that these three hybrids have excellent feed quality because the NDF (Neutral Detergent Fiber) is decreased by $7{\sim}3.8%$ comparing that the NDF of Hwasan 101 is 58.3%. Also each hybrids contains more crude fat by 1%, a little more chemical composition and less ash contents. Thus we can tern that chemical composition contents of these hybrids are also fittable.

• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.317-322
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• 1995

As a basic research for breeding new varieties, reciprocal -intergeneric crosses between Citrus junos and P.trifoliata were made. F$_1$ hybrid production using in vitro ovule culture, gametogenesis, and fertilization phenomena were investigated. Frequency of fruit set resulting from crossing of Citrus junos and Poncirus Trifoliata was 16.6% while that of Poncirus Trifoliata and Citrus junos was 11.7%. Callus formation occurred well when ovules at the 6th week after pollination were cultured on MT (Murashige and Tucker) medium supplemented with zeatin 0.5 mg/L and NAA 1.0 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4-D 0.1 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4 D 0.1 or 0.5 mg/L. The invitro germination rates of 20-week-old ovules set C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 54.5% and 48.6%, respectively. The emergence ratios of trifoliate hybrids obtained by C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 56.7% and 100%, respectively. The chromosome number of C. junos and P. Trifoliata was n = 9 or 2n = 18, and the sizes of their pollen grain were 33.75 $\mu$ and 25.0 $\mu$. The length and width of embryo sac in C. junos and P. Trifoliata were 69.38~79.23 $\mu$ and 27.50~38.56 $\mu$, and those of egg cells were 17.50~41.50 $\mu$ and 6.25~8.12$\mu$. Fertilization of C. junos and P. trifoliata terminated 72 h after pollination.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.14
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• pp.165-172
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• 1973

The sterile phenomenon is frequently found in the inter-species hybrids of ginseng as in other plants. It is known that among the hybrids between Panax Ginseng (PG) and Panax Quinquefolium (PQ), and between Panax Ginseng and Paxax Japonicus (PI), PG${\times}$PI is fertile only very rarely, while PG ${\times}$ PQ is always sterile. Therefore, in order to clarify the relationship between this sterility phenomenon and the metabolism of free amino acids, the changes of free amino acids through the formation of the flower organs and seeds of two hybrids, PG ${\times}$ PQ and PG ${\times}$ PI were investigated by thin layer chromatography. The results are summarized as follows: 1. Distinct differences in the quantity and number of free amino acids were recognized between PG ${\times}$ PQ, PG ${\times}$ PI and their parent plants. From the hybrid PG ${\times}$ PQ, 19 kinds of ninhyrin sensitive substances were detected in all. They were (1) 17 amino acids: alanine, valine, leucine, phenylalanine, proline, hydroxy-proline, serine, threonine, tyrosine, aspartic acid, glutamic acid, lysine, arginine, ${\gamma}$-amino butyric acid, ${\beta}$-alanine, cysteic acid and tryptophan, and (2) two amides: asparagine and glutamine. From the hybrid PG ${\times}$ PI, in addition to the above 19 substances, methionine and one unknown substance were detected. 2. Generally, alanine, as partie acid, glutamic acid, cysteic acid and asparagine were detected in large amounts in the two hybrids as in PG, PG and PJ but it was a noticeable fact concerning these two hybrids that the largest quantity of asparagine was found at microspore satge and pollen mature stage. 3. The decrease of cysteic acid in the two hybrids at the red ripened stage was the same as in PQ and PJ but opposite to the change in PG. The detection of methionine in PG ${\times}$ PJ was worthy of notice. 4. The change of proline was conspicuously different from that in their parent plants. It was detected as a trace of color at the micros pore stage while asparagine was detected in the greatest amount at that time. It is well known that the quantity of proline is closely related to the sterility of plant. This fact was also found true in the formation of ginseng seeds. It was reported as well that asparagine accumulated when proline decreased. 5. The deficiency of proline seemed to be closely related with the sterility of hybrids and with the degradation of pollen in anther. 6. The difference in the changes of free amino acids between the selfed lines of PG, PQ and PJ, and their hybrids seemed to be caused by the transformation of gene-action system by hybridization. On these phenomena along with proline metabolim and its physiological role in seed formation further studies are required.