• Journal of Veterinary Clinics
• /
• v.22 no.4
• /
• pp.314-317
• /
• 2005

The potential therapeutic effect of ozone gas on bovine mastitis was investigated. Eighteen quarters from 18 lactating cows with chronic mastitis were included. The 18 quarters were assigned to the control group (treatment with antibiotics for 3 days), experimental group I (0.1 ppm ozone treatment, for 7 days) and experimental group II (1ppm ozone treatment, for 3days). In experimental group I, milk somatic cell counts were lower on day 7 after ozone treatment, compared to the pretreated counts, but were higher than the control counts. In experimental group II, somatic cell counts were significantly decreased (p<0.05) on day 7 compared to the pretreatment counts, and they were lower than the control counts. There were no changes in leukocyte, neutrophil, or lymphocyte numbers, N/L ratios, N/L ratios, or serum total protein in the control and experimental groups. We concluded that ozone gas treatment(1ppm for 3days) might be effective for treatment of bovine mastitis.

• Journal of the korean veterinary medical association
• /
• v.46 no.6
• /
• pp.527-543
• /
• 2010

구제역은 소 돼지 사슴 면양 산양 고라니 노루 등 우제류 동물에서 발생하는 제1종 가축전염병으로 빠른 시간내에 동물의 입 발굽 젖꼭지 등에 수포를 형성하는 것이 특징이다. 감염된 소의 혓바닥, 잇몸, 입술, 입 내부점막, 주둥이에 크기가 다양한 수포가 형성된다. 심한 경우에 혀 전체 넓은 부위에서 궤양이 형성될 수 있다. 수포는 24시간이내에 정상적으로는 파열되며, 그 수포액은 $10^8/ml$의 역가를 갖는 바이러스를 함유한다. 상피가 떨어지고, 유연이 심해지며, 타액이 입 주변에 흔건히 흘러내린다. 입 주변 병변과 동시 또는 이어서 수포가 발굽에 형성되며, 부분적으로 발굽갓띠 또는 제부의 갈라진 틈에서 생성되어 통증과 파행의 원인이 된다. 수포는 보통 유두와 유방에 발생되며, 때로는 세균성 유방염을 발생시킨다. 생산성의 감소는 보통 25% 정도로 추정된다. 돼지는 초기에 파행, 발열, 의기소침, 식욕부진이 있다. 소 보다 발굽에서 더욱 증상이 심하다. 유두, 유선, 콧등에 수포가 형성된다. 혀의 병변은 소보다 심하지 않다. 잠복기가 2~14일 (소에서의 약 6.2일, 돼지에서는 약 10.2일)로 짧고, 전파가 매우 빠르며 경제적 피해가 크기 때문에 국제 교역에서도 규제대상이 되는 질병이다. 구제역의 병인체는 피코르나바이러스과 아프토바이러스속에 속하는 구제역 바이러스로 현재까지 7개의 혈청형(A, O, C, Asia 1, SAT 1, SAT 2 및 SAT 3)이 알려져 있다. 이 전염병은 동물전염병 중에서 가장 악명 높은 전염병이다. 세계동물보건기구 (OIE)에서도 각 국간에 국제적인 위협을 최소화하기 위하여 질병의 발생을 보고해야 하는 질병으로 특별 관리하고 있다. 2006년 이후 최근까지 아시아 지역에서 지속적으로 많은 발생을 보이고 있다. 금년 들어 중국, 대만, 일본, 몽골, 우리나라까지 발생되어 이 질병의 위험성에 대하여 다시 한번 생각하게 하였다. 구제역의 재발방지를 위하여 최근 동북아시아 지역의 발생상황과 향후 구제역의 발생억제 또는 발생시 긴급 대응을 위하여 해야 할 일은 무엇인지 알아보자.

• Microbiology and Biotechnology Letters
• /
• v.28 no.4
• /
• pp.189-194
• /
• 2000

Microorganisms causing bovine mastitis, swine diarrhea and swine pneumonia were isolated from farms in Chungbuk and Kyunggi Province and the isolates were identified using microbial identification system(MIS). The most common isolates from bovine mastitis were Staphylococcus sp.,Streptococcus sp., and Corynebacterium sp., those from swine diarrhea were Escherichia coli, Salmonella sp., and Clostridium perfringens, those from swine pneumonia were Bordetella bronchiseptica and Pasteurella multocida. Examination of 16 antibiotics against these pathogens revealed that the incidence of antibiotic-resistant microoganisms were very high and that many of these isolates had multiple resistance to various commercially available antibiotics such as penicillin, ampicillin, erythromycin, streptomycin, norfloxacin, and tetracycline. These results suggest that the use of antibiotics in a farm should be controlled in order to decrease the number of antibiotic-resistant microorganisms were very high and that many of these isolates had multiple resistance to various commercially available antibiotics such as penicillin, ampicillin, erythromycin, streptomycin, norfloxacin, and tetracycline. These results suggest that the use of antibiotics in a farm should be controlled in order to decrease the number of antibiotic-resistant microorganisms.

• Journal of Veterinary Clinics
• /
• v.22 no.4
• /
• pp.318-321
• /
• 2005

Forty- nine quarters from 24 lactating cows with chronic mastitis were selected. The cows were raised on dairy farms in Gongju, Jochiwon and Yeongi in Chungnam province, and Iksan in Jeonbuk province, Korea. The 49 quarters with bovine mastitis were divided into control (7 quarters) and experimental (42 quarters) groups. The experimental quarters were assigned to experimental group A (10 quarters, somatic cell count: $50-100\times10^4/ml)$, experimental group B (14 quarters, somatic cells count: $100-300{\times}10^4/ml)$, and experimental group C (18 quarters, somatic cells count: $>300\times10^4/ml$), according to the number of the somatic cells in their milk. The quarters of control group were treated with norfloxacin ointment (10 g/tube) based on the result of sensitivity, twice a day for 3 days. The quarters or experimental groups were infused 10ml or ozonated oils twice a day for 3 days. After treatment, the milk of the control group contained non-significantly lower numbers of somatic cells and bacteria on day 7, compared with pretreatment levels. Experimental groups A, B and C had lower somatic and bacterial cells in their milk on day 7, compared with pretreatment levels. Experimental group B and C had significantly lower numbers of somatic cells in their milk ell day 7 than before treatment (p<0.01). However, no significant difference in somatic cell numbers was detected between the control alld experimental groups. It was concluded that ozone therapy with ozonated oil applied on bovine mastitis might be effective.

• Feed Journal
• /
• v.4 no.10 s.38
• /
• pp.132-137
• /
• 2006

목장 경영의 성공과 실패에는 여러 가지 요인이 작용하지만 연중 얼마나 적은 소가 도태되는가 하는 것 또한 아주 중요하게 생각해 보아야 할 사항이다. 낙농에 있어 거의 대부분의 질병이 산욕기에 발병하는데 산욕기(産褥期)란 분만으로 인한 상처가 완전히 낫고, 자궁이 평상시 상태가 되며 신체의 각기관이 임신 전의 상태로 회복되기까지의 기간으로 대개 분만 후 6~8주간을 말한다. 산욕기에 주로 발생할 수 있는 다양한 질병(산욕기 부전마비, 난산, 급성유방염, 제4위 전위증 등)들이 조기에 발견되어 적절히 치료되지 못하고 만성화되면서 전해질불균형, 에너지와 단백질의 섭취부족에 의한 영양장애로 허약과 지방간 및 케토시스 등으로 진행되어 결국 도태됨으로서 목장 경영에 있어 막대한 영향을 끼치는 것이다. 이러한 산욕기 질병의 주 원인은 건유기 사양관리에 있다. 건유기간 동안 체점수(BCS)의 변화를 최대한 줄이면서 분만전의 스트레스를 얼마만큼 줄일 수 있도록 관리하는가에 달려있다. 다시말해 분만 전 마지막 한달간에 건유에서 분만으로의 이행과정을 얼마나 매끄럽게 연결 지어주는가에 따라 산욕기 질병의 발생률이 결정되는 것이다. 본 고에서는 건유기 사양관리 소홀로 발생되는 산욕기 부전마비에 관해서 자세히 설명해 보고자 한다.

• Food Science of Animal Resources
• /
• v.28 no.4
• /
• pp.457-462
• /
• 2008

Lactococcus lactis NK34, isolated from jeotgal (Korean traditional fermented fish), produces bacteriocin against bovine mastitis pathogens such as Staphylococcus aureus 7, S. aureus 8, Staphylococcus chromogenes 10, S. chromogenes 19, Staphylococcus hominis 9, Streptococcus uberis E290, Enterococcus faecium E372, Streptococcus agalactiae ATCC 13813, Pseudonocardia autotrophia KCTC 9455, and Staphylococcus simulans 78. Lacticin NK34 was inactivated by protease XIV but not by protease IX, protease XIII, proteinase K, $\acute{a}$-chymotrypsin, trypsin, and pepsin. Also, lacticin NK34 was stable over a pH range of 2 to 9 for 4 hr and withstood exposure to temperatures of 30-$100^{\circ}C$ for 30 min. Lacticin NK34 showed bactericidal effects against S. simulans 78. This bacteriocin was purified using ammonium sulfate precipitation, ion exchange chromatography, ultrafiltration, and hydrophobic chromatography. Tricin-SDS-PAGE of purified bacteriocin gave the same molecular weight (3.5 kDa) as nisin. The gene encoding this bacteriocin was amplified by PCR using nisin gene-specific primers. It showed similar sequences to this nisin Z gene. These results indicate that lacticin NK34 is a nisin-like bacteriocin, and could be used as an antimicrobial alternative for livestock.

• Korean Journal of Veterinary Research
• /
• v.41 no.2
• /
• pp.157-165
• /
• 2001

Amplified fragment length polymorphism(AFLP) technique is based on the polymorphism detection through selective PCR amplification of restriction fragments from digested genomic DNA and thus includes the procedures of the total DNA digestion by endonucleases, ligation of adapters to the ends of the fragments, and following the selective amplification of the restricted DNA fragments. This study were aimed to : (1) determine the genetic variability of S aureus strains, (2) estimate genetic diversity within and among these strains, (3) compare phylogenetic relationships among these strains as genetic markers using AFLP techniques. Genomic DNA was digested with a particular combination of three restriction enzymes with specific recognition sites and the DNA fragments were ligated to restriction specific adapters and amplified using the selective primer combinations. In the S aureus strain, the number of scorable AFLP bands detected per each primer combination varied from 29 to 102, with an average of 61.59 using 27 primer combinations. A total of 1,663 markers were generated, 904 bands of which were polymorphic, showing a 33.48% level of polymorphism with these primer combinations. Among the primer combinations, E02/T02, E02/T03, E04/H02, E02/T01 and E04/H03 primer combinations showed a high level of polymorphism with 0.78, 0.76, 0.74, 0.71 and 0.70, respectively. But T03/H01, E01/T02 and E01/T03 primer combinations showed a low level of polymorphism with 0.38, 0.37 and 0.15, respectively, Therefore, the former primer combinations will be the most effective for AFLP analysis of S aureus. In SA1 sub-types the level of polymorphism of S aureus KCTC 1927 was similar to that of S aureus CU 01(0.825) and higher than those of other strains such as S aureus CU 02 (0.715), S aureus KCTC 2199(0.625), S aureus KCTC 1916(0.607) and S aureus KCTC 1621 (0.553). In SA2 sub-types the level of polymorphism of S aureus CU 07 was similar to that of S aureus CU 08(0.935) and higher than those of both S aureus CU 04(0.883) and S aureus CU 05(0.883) and lower than those of S aureus CU 03(0.583). In SA3 subtypes the level of polymorphism of S aureus CU 11 was similar to that of S aureus CU 12(0.913) and lower than that of S aureus CU 15(0.623). The results proved that AFLP marker analysis of S aureus strain could be used to study the epidemiology of mastitis and in addition, common genotype in geographic region could be useful for the development of an effective vaccine or DNA marker for easy diagnosis of mastitis caused by S aureus infection.

• Korean Journal of Veterinary Service
• /
• v.44 no.3
• /
• pp.169-174
• /
• 2021

Mastitis is an inflammatory condition of the mammary gland, most often caused by bacterial infections, resulting in significant economic losses to the dairy industry. Antimicrobial resistance has been of great concern because of the extensive clinical use of antibiotics. For this reason, the development of new compounds as an alternative treatment to bovine mastitis is needed. Bee venom has been widely used as an oriental treatment for several inflammatory diseases and bacterial infections. The aim of the present study was to evaluate the antimicrobial activity of bee venom on bacteria isolated from bovine mastitis. A total of 107 isolates from bovine mastitic milk samples collected in 2019 and 2020 in Jeonbuk province. All bacterial isolates were tested for susceptibility to bee venom of the honey bee (Apis mellifera). In order to obtain comprehensive antibacterial activities of the bee venom, we measured the minimal inhibitory concentration (MIC) of the bee venom against bacterial strains. Bee venom showed significant inhibition of bacterial growth of Gram-negative bacteria Citrobacter spp., Escherchia coli, Klebsiella spp., Pseudomonas spp., Serratia spp. and Raoultella with MIC values of 96, 81, 72, 230, and 85 ㎍/mL, respectively, and Gram-positive bacterial Enterococcus spp., Staphylococcus spp. and Streptococcus spp. with MIC values of 29, 21 and 16 ㎍/mL, respectively. The results indicated that the MIC values were different depending on the bacterial strains, and those of Gram-positive bacteria were lower than those of Gram-negative bacteria for bee venom. These findings suggested that bee venom could be an effective antimicrobial treatment for bovine mastitis; however, further research is necessary to evaluate the mechanism underlying the antimicrobial action, its effectiveness/safety in vivo and effective application for therapeutic use.