• Title/Summary/Keyword: 소독법

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Bactericidal Efficacy of a Powder Disinfectant Containing Phosphate Compounds Against Salmonella Typhimurium

  • Park, Eun-Kee;Cha, Chun-Nam;Yoo, Chang-Yeul;Kim, Suk;Lee, Hu-Jang
    • Journal of Food Hygiene and Safety
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    • v.31 no.6
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    • pp.471-475
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    • 2016
  • Salmonella Typhimurium (S. Typhimurium) is one of the common food pathogens which may cause gastroenteritidis in human and animals. The microorganism also causes the economic loss in animal farming and food industry. In this study, the disinfection efficacy of a powder disinfectant containing phosphate compounds as a main ingredient, was evaluated against S. Typhimurium. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. The disinfectant and test bacteria were diluted with hard water (HW) or organic matter suspension (OM) according to treatment condition. On HW and OM conditions, the bactericidal activity of the disinfectant against S. Typhimurium was 4- and 2-fold dilutions, respectively. As the disinfectant composed to phosphate compounds possesses bactericidal efficacy against pathogenic bacteria such as S. Typhimurium, the powder disinfectant can be used to control the spread of bacterial diseases.

Studies on the development of seed disinfectant in non-mercurious compounds (비유기수은 종자소독제개발에 관한 연구)

  • Lee Du-Hyung
    • Korean journal of applied entomology
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    • v.18 no.2 s.39
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    • pp.63-71
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    • 1979
  • Tests were made to find new non-mercurious seed disinfectants that were best for rice seeds. For these experiments four seed samples carr)?ing natural infection of Pyricularia oryzae, Helminthosporium oryzae, and Fusarium moniliforme were used and the following fungicides were used; Zinc Omadine, Sodium Omadine, Panoctine, Tecto-F, Benlate-T, Homai, Sisthane, $P_{242}$, Busan 30, Tecto-Wp and Terracoat Zn. Blotter method and water agar plate method used in the laboratory and growing-on test used in greenhouse. Results have shown that Sisthane, $P_{242}$ and Sodium Omadine have equal or superior effect to organic mercury compound against P. oryzae, H. oryzae, and F. moniliforme. Benlate T and Homai are effective against blast and Bakanae disease, but are inferior to organic mercury compound against brown spot disease. Busan 30 and Panoctine are effective against blast and brown spot disease, but have moderately inferior effects against F. monilifome. It is considered that the recommendable testing methods of seed treatment were blotter method for P. oryzae and water agar plate method for H, oryzae and F. moniliforme according to the experimental results obtained. No phytotoxicity against seed germination and seedling growth were observed when treated with disinfectants before germination of seeds.

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Evaluation of Possibility of using an Ultrasound Probe Sterilizer using the Steam Fumigation Method (증기훈증방식을 이용한 초음파 프로브 소독기의 사용 가능성 평가)

  • Ha, Jeong-Min;Heo, Yeong-Cheol;Han, Dong-Kyoon
    • Journal of the Korean Society of Radiology
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    • v.16 no.3
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    • pp.195-201
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    • 2022
  • This study aims to evaluate the feasibility of the disinfection of clinical ultrasound probes using the vapor fumigation method, which can quickly achieve high-level disinfection. Upon the inspection of the microbial contamination level of clinically used ultrasound probes, nine different types of bacteria were detected. The disinfection efficacy of 7.5% and 35% hydrogen peroxide (H2O2) was comparatively tested for the detected microbes. The 35% H2O2 demonstrated superior efficacy per disinfection duration. No significant change was observed in the rubber component of the ultrasound probes as a result of the 35% H2O2 disinfection treatment. The probes were contaminated with the microbes detected in the microbial contamination level inspection and subsequently disinfected using the novel medical disinfector that utilizes the vapor fumigation method. As a result, the disinfection using the novel device achieved 100% eradication of the microbes from the probes.This study demonstrates that the novel vapor fumigation method-based disinfector provides a faster and more powerful means of disinfection than the conventional disinfection methods. Therefore, the novel disinfector has the potential to be used as a convenient ultrasound probe disinfector in clinical settings.

Development of Drainage Water Disinfection System by Electric Shock in Recirculating Soilless Culture (순환식 수경재배에서 배액의 전기충격살균법 연구)

  • Lee, Mun Haeng;Kim, Sung Eun;Lee, Sang Don;Lee, Jae Eun;Kim, Hak Sun;Cho, Suk Keong;Sim, Sang Youn;Kim, Young Shik
    • Journal of Bio-Environment Control
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    • v.25 no.1
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    • pp.49-56
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    • 2016
  • This study was conducted to develop the disinfection system using electric shock for recycled nutrient solution in recycling soilless culture. Stainless steel (SUS 316) was found as the most appropriate electrode material for electrical disinfection system from the view of high electrical conductivity, low electric resistance, and low price. There were no changes in nutritional elements when the electric shock passed through the nutrient solution by stainless steel electrode. The amount of electric current increased with width than thickness of the electrode. The farther the distance between the electrodes was increased the time to reach out the aimed amount of current. The electric shock was applied to Ralstonia solanacearum and Fusarium oxysporum as representative bacteria and also fungi. Any of those pathogens were killed with the percentage of higher than 97% in the condition of 15VDC or 24VDC.

Survey on Practical use of Sanitizers and Disinfectants on Food Utensils in Institutional Foodservice (단체급식소의 기구등의 살균소독제 사용실태 조사)

  • Lee, Yu-Si;Lee, Seong-Hee;Ryu, Kyung;Kim, Yong-Soo;Kim, Hyung-Il;Choi, Hyun-Chul;Jeon, Dae-Hoon;Lee, Young-Ja;Ha, Sang-Do
    • Journal of Food Hygiene and Safety
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    • v.22 no.4
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    • pp.338-345
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    • 2007
  • This study surveyed on the actual conditions of using sanitizers and disinfectants for improvements of sanitization on food utensils at 105 school and 20 industry foodservice operations. The questionnaire which was administered to 125 foodservices was used as a mail or visitation method. The answers of asking "Perception on temporary authorization system of sanitizers and disinfectants on food utensils" were 75% in contract managed school foodservices, 81.8% in self operated school foodservices, and 50% in industry. Main factors to choose sanitizers were sterilizing power (38.6%, 28.6%, 38.9%) and safety (32.6%, 46.1%, 33.3%) at every foodservices. Keeping ratio of sanitizers and disinfectants guidelines in contract managed school, self operated school and industry foodservices were 64.8%, 52% and 73.7%, respectively. If easy and practical guideline is developed, most foodservices replied to use if for disinfection of foodservices. Most of the foodservices were not only knowing sanitizers and disinfectants but also possessing a guideline. However, they didn't perform disinfection according to the guideline due to its complexity. Consequently, we suggest that it is necessary to provide an easy and practical "sanitizers and disinfectants guideline" and useful information.

THE EFFECT OF NAOCL TREATMENT AND STERILIZATION PROCEDURES ON THE CORROSION OF ENDODONTIC FILES (차아염소산나트륨 처리와 멸균법이 근관 치료용 파일의 부식에 미치는 영향에 관한 연구)

  • Yang, Won-Kyung;Ra, Yoon-Sik;Lee , Young-Kyoo;Son, Ho-Hyun;Kim, Mi-Ri
    • Restorative Dentistry and Endodontics
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    • v.30 no.2
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    • pp.121-127
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    • 2005
  • A variety files made of stainless steel (S-S) or nickel-titanium (Ni-Ti) are used during endodontic treatment. The purpose of tt)is study was to evaluate the corrosion susceptibility of S-S and Ni-Ti endodontic files. Three brands of files were used for this study: $K-flex^{(R)}$ S-S files (Maillefer, USA), $Profile^{(R)}$ Ni-Ti files (Maillefer, USA), $K-3^{(R)}$ Ni-Ti files (SybronEndo. USA). 120 files of each brands (21mm, ISO size $\#20$) were divided into 12 groups according to 1) sterilization methods using Autoclave or Ethylene Oxide (E-O) gas, 2) Irrigation solutions using $5.25\%$ NaOCl or Saline, 3) the number of sterilization (1, 5, 10 times), After above procedures, each of the files was inspected by three examiners with a light microscope and camera at X25. Each file was judged and ranked according to the following criteria: 0;, no corrosion, 1; mild corrosion, 2; moderate corrosion, and 3; severe corrosion. The files of high score were examined under the Scanning Electron Microscope. Data were statistically analyzed with the Kruskal-Wallis test (p < 0.05). Most of the ten time-autoclaved files had showed mild to moderate corrosion. But, one or five time-autoclaved files did not show corrosive surface. NaOCl treatment and E-O gas sterilization did not influence on corrosion. There was a significant difference in corrosion susceptibility between sterilization methods and the number of autoclaving. However, there was no significant difference between brands and file materials.

Chemical Control of Rhizome Rot of Ginger by Seed-Rhizome and Soil Treetment (생강 근경부패병의 약제방제)

  • Choi, Jae Eul
    • Korean Journal of Agricultural Science
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    • v.26 no.1
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    • pp.1-5
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    • 1999
  • This experiment was carried out to define the development of control method against rhizome rot of ginger. It was difficult to control of rhizome rot of ginger completely by way of only rhizome sterlization. When soil were stenilized with dazomet, and metalacxyl granule was applied, higher than 95% of control value as well as 39% of yield increase compared to control were found. Soil drenching with metalacxyl cupper oxychloride and streptomycin controlled the rhizome rot satisfactorily, but was inferior to the above mentioned combination.

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Establishing Test Method of Sporicidal activity of Commercial Sterilants (아포살균용 살균소독제 유효성 평가방법 확립)

  • Kim, Hyung-Il;Jeon, Dae-Hoon;Yoon, Hae-Jung;Kwak, In-Shin;Eom, Mi-Ok;Sung, Jun-Hyun;Park, Na-Young;Won, Sun-Ah;Bae, Seo-Young;Lee, Young-Ja;Kim, So-Hee
    • Journal of Food Hygiene and Safety
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    • v.24 no.4
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    • pp.312-317
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    • 2009
  • Usually, bacterial spores are hundreds or thousands of times more resistant to chemical sanitizers than are vegetable bacteria. Consequently, it is hard to assess whether a commercial sterilant, containing hydrogen peroxide and peracetic acid as ingredients, has or does not have sporicidal activity under certain conditions using the National Standard Test Method for assessing bactericidal activity against Escherichia coli and Staphylococcus aureus. Hence we established alternative the standard test method and requirements to determine whether they are effective in showing at least reduction of $10^3$ in the number of Bacillus subtilis ATCC 6633 spores under the required test condition for evaluation of sporicidal activity including verification methodology. This standardized method has proved to be suitable for evaluating effectiveness of commercial sterilants and could be used as Standardization Test Method for assessing sporicidal activity.