• Title/Summary/Keyword: 세포융합기술

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Protective Effects of Trifolium pratense L. Extract against H2O2-induced Oxidative Stress in HaCaT Keratinocytes (인간 피부각질세포에서 Hydrogen peroxide로 유도된 산화적 스트레스에 대한 붉은 토끼풀 추출물의 세포 보호 효과)

  • Mi Song Shin;You Kyeong Lee;Seo Young Choi;Ji Sun Hwang;Parkyong Song;Hyeon Cheal Park;Keun Ki Kim;Hong-Joo Son;Yu-Jin Kim;Kwang Min Lee
    • Journal of the Korean Applied Science and Technology
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    • v.40 no.2
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    • pp.223-232
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    • 2023
  • Oxidative stress plays a significant role in the pathogenesis of various skin conditions, resulting in cellular and tissue damage that can contribute to the development of skin tone unevenness, roughness and wrinkles. In this study, we found that Trifolium pratense L. extract (TE) attenuated oxidative-induced damage in HaCaT cells and elucidated the underlying molecular mechanism. Our finding demonstrated that TE effectively protected HaCaT cells against H2O2-induced cell death by inhibiting caspase-3 activation, downregulating Bax and upregulating Bcl-2, and attenuating the activation of three mitogen-activated protein kinases (MAPKs). Our results suggest that TE has remarkable cytoprotective properties against oxidative damage in HaCaT cells and could serve as a complementary or alternative approach to prevent and treat skin damage.

Antioxidant and nitric oxide inhibition effect of domestic and foreign fermented black tea extracts (국내외 홍차 추출물의 항산화 효과 및 Nitric Oxide 생성 저해 효과)

  • Moon, Gyo-Ha;Kim, Gyeong-Ji;Lee, Yu-Rim;Kim, Jong Cheol;Shim, Doobo;Chung, Kang-Hyun;Lee, Kwon-Jai;An, Jeung Hee
    • Korean Journal of Food Science and Technology
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    • v.53 no.4
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    • pp.454-462
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    • 2021
  • This study investigated the antioxidant and nitric oxide (NO) inhibition effects of three domestic and seven foreign fermented black tea 70% ethanol extracts. Bosung showed the highest total polyphenol and tannin contents. Jeju showed the highest total flavonoid contents. The theanine content was higher in Hadong (651.50 mg%) than in foreign fermented black tea. At 25 ㎍/mL, the domestic fermented black tea extract showed the highest DPPH and ABTS racial-scavenging activities, reducing power assays. Domestic fermented black tea showed higher NO inhibitor activity than foreign black tea at 50 ㎍/mL. Bosung black tea extract showed an increase in SOD-1 level (1.39-fold) compared to the LPS-only group. Bosung and Jeju decreased the GST protein by 1.52- and 1.46-folds, respectively, compared to the LPS-only group. Thus our results suggest that domestic fermented black tea (Bosung, Jeju, and Hadong) are effective antioxidants in RAW 264.7 cells.

ICT Convergenced Cascade-type Incubator for mass production of microalgae (미세조류 대량생산을 위한 ICT 융합 계단식 연속 배양 장치)

  • Lee, Geon Woo;Lee, Yong Bok;Yoo, Yong Jin;Baek, Dong Hyun;Kim, Jin Woo;Kim, Ho Seob
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.5
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    • pp.379-386
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    • 2021
  • This study was undertaken to develop a cascade-type continuous culture system (CCCS) that combines both ICT and biotechnology (BT), for the mass production of microalgae. This system is capable of maintaining the essential culture conditions of pH, temperature, carbon dioxide, and illuminance control, which are key parameters for the growth of microalgae, and is economical for producing microalgae regardless of the season or location. It has the added advantage of providing stable and high productivity. In the current study, this system was applied to culture microalgae for 71 days, with subsequent analysis of the experimental data. The initial O.D. of the culture measured from incubator 1 was 0.006. On the 71st day of culture, the O.D.s obtained were 0.399 (incubator 1), 0.961 (incubator 2), 0.795 (incubator 3), and 0.438 (incubator 4), thereby confirming the establishment of continuous culture. Thus, we present a smart-farm based on ISMC (in-situ monitoring and control) for a mass culture method. We believe that this developed technology is suitable for commercialization, and has the potential to be applied to hydroponics-based cultivation of microalgae and cultivation of high-value-added medicinal plants as well as other plants used in functional foods, cosmetics, and medical materials.

Study of Plant Growth Inhibition with Edible Sweetener Saccharin and Acesulfame Potassium (식용 감미료 사카린과 에이스셜팜 칼륨의 식물 성장 저해 연구)

  • Donggiun Kim
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.5
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    • pp.661-667
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    • 2023
  • Five commercially available edible sweeteners are used as diet products because they can replace sucrose. In studies on the effects on animals and the human body, stability has been proven by excreting-oriented studies with characteristics of animal cells, and accumulation in small amounts has been ignored. On the other hand, plants can absorb, degrade, and accumulate foreign substances, so the effect of degradability and accumulation potential can be studied using plants. Metabolic effects in plants of commercially available saccharin and acesulfame potassium (Ace K) were tested using germinated barley and bean sprouts. In germinated barley and bean sprouts, saccharin and ace K showed inhibitory effects on plant growth in all organs from low concentrations in leaves, stems and roots. In addition, it can be observed that the symptoms of death appear clearly over time, so it can be seen that they are accumulated in the body of the plant. As the accumulated amount increases, the toxic effect increases and the plant reaches a state where it is unable to metabolize, turning black from the tip of the leaf and reaching a state of death. In order to remove the accumulated artificial sweetener, recovery was attempted by culturing in distilled water, but it acts as a substance that is not degraded and dies without avoiding toxicity. Saccharin and ace K cannot be excreted from the cell. Its toxic effects are thought to be persistent, inhibiting growth and eventually leading to cell death.

An Efficient Method for Production of Extracellular Human Tissue Factor in Escherichia coli (인간조직인자 세포외 부분의 효과적인 제조 방법)

  • Yoo, Hwan-Goo;Park, Yang-Jin;Lee, Woo-Yiel
    • Journal of Life Science
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    • v.19 no.5
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    • pp.561-565
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    • 2009
  • Human Tissue factor is an essential enzyme activator that forms a catalytic complex with factor VII/ VIIa, and catalyzes both the extrinsic and intrinsic blood coagulation cascades. The extracellular domain of human tissue factor is responsible for association with the biological partner. The efficient procedures for preparing biologically active human tissue factor are essential for the preclinical and clinical studies with coaguligands. An expression vector in Escherichia coli has been constructed to direct the production of extracellular human tissue factor without a fusion protein or a $His_6$ at the N-terminus. The recombinant human tissue factor was expressed in large amounts as a non-native state in E. coli. The recombinant protein was simply renatured during the DEAE-sephacel chromatographic purification procedure. Our expression and purification system does not require a protease treatment or an additional chromatographic step to remove a fusion contaminant, which provides a very useful alternative to conventional expression systems for the production of human tissue factor.

다공성 타이타늄 지지체의 생체적합성에 관한 연구

  • Kim, Seung-Eon;Hyeon, Yong-Taek;Yun, Hui-Suk;Heo, Su-Jin;Lee, Si-U;Sin, Jeong-Uk;Kim, Yeong-Gon
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.05a
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    • pp.49.1-49.1
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    • 2009
  • 최근 손상된 생체조직의 재생 또는 대체를 위하여 다공성의 지지체(scaffold)를 이용하는 연구가 활발히 이루어져 왔다. 지지체 재료는 조직 재생을 목적으로 하는 경우에는 생분해성 고분자, 생흡수성 세라믹스 또는 이들의 복합재료가 사용되고, 조직 대체를 목적으로 하는 경우에는 금속 또는 세라믹스 재료가 단독으로 사용된다. 현재 경조직 대체를 위한 임플란트 재료로 사용되고 있는 금속재료 중 대부분이 타이타늄 또는 타이타늄 합금이다. 타이타늄은 비강도, 내식성이 우수하며, 생체 내 환경에서 부동태피막 재생 속도가 빠르고, 섬유상 결체조직 형성 두께가 얇아 생체의료용 소재로서 각광을 받고 있다. 다공성 타이타늄은 기존 타이타늄 소재의 장점에 다공체의 구조적인 특성을 부가하여 하중을 받는 골 결손부에 사용될 경우 뼈와의 탄성계수 차이에서 기인하는 응력차폐(stress shielding) 효과를 최소화할 수 있고, 다공체 내부로 골조직 성장을 유도할 수 있어 지지체와 골조직이 일체화되는 골융합 효과의 극대화를 기대할 수 있다. 본 연구에서는 기공 구조를 다양하게 제어할 수 있고, 3차원적 연결 기공구조를 만들 수 있는 적층조형(layer manufacturing) 기술을 이용하여 3차원 다공성 타이타늄 지지체를 제조하였으며, 이에 대한 세포독성, 조골세포 증식능 등 in vitro 생체적합성을 평가하고, Rat model 을 이용한 in vivo 생체적합성을 평가하였다. 또한 지지체의 골조직 재생 유도성의 증대를 위한 생체활성처리 영향도 분석 평가하였다.

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Overexpression of the Metal Transport Protein1 gene (MTP1) in Arabidopsis Increased tolerance by expression site (금속전달 유전자(MTP1)의 과발현 애기장대에서 발현 위치에 따른 내성 증가 연구)

  • Kim, Donggiun
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.3
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    • pp.327-332
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    • 2019
  • Today's scientists try to remove heavy metals with many new technologies such as phytoremediation. One of the best cutting edge technologies is developing transgenic plants to remove certain heavy metal in soil. I constructed the transformation vector expressing T. goesingense Metal Transport Protein1 gene and TgMTP1: GFP genes. The transgenic plants were selected and confirmed the transformed genes into Arabidopsis thaliana genome. Expression was confirmed in several parts in Arabidopsis cells, tissues and organs. When TgMTP1 overexpressing Arabidopsis thaliana were subjected, transgenic plants showed higher heavy metal tolerance than non-transgenic. For further study I selected the transgenic plant lines with enhanced tolerance against four different heavy metals; Zn, Ni, Co, Cd. The accumulation of these metals in these plants was further analyzed. The TgMTP1 overexpressing Arabidopsis thaliana plant of selected lines are resistant against heavy metals. This plant is characterized by the expression of the MTP1 gene accumulating heavy metal in the vacuole and being simultaneously expressed on the plasma membrane. In conclusion, these plants may be used in plant purification applications, and as a plant with increased tolerance.

Large scale enzymatic production of chitooligosaccharides and their biological activities (키토산올리고당의 효소적 대량생산 및 생리활성)

  • Kim, Se-Kwon;Shin, Kyung-Hoon
    • Food Science and Industry
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    • v.53 no.1
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    • pp.2-32
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    • 2020
  • In recent years, significant importance has been given to chitooligosaccharides (COS) due to its potent notable biological applications. COS can be derived from chitosan which is commonly produced by partially hydrolyzed products from crustacean shells. In order to produce COS, there are several approaches including chemical and enzymatic methods which are the two most common choices. In this regard, several new methods were intended to be promoted which use the enzymatic hydrolysis with a lower cost and desired properties. Hence, the dual reactor system has gained more attention than other newly developed technologies. Enzymatic hydrolysis derived COS possesses important biological activities such as anticancer, antioxidant, anti-hypersentive, anti-dementia (Altzheimer's disease), anti-diabeties, anti-allergy, anti-inflammatory, etc. Results strongly suggest that properties of COS can be potential materials for nutraceutical, pharmaceutical, and cosmeceutical product development.

Spermiogenesis in the Korean long-Fingered Bat (Miniopterus schreibersi fuliginosus) (한국산 긴날개 박쥐(Miniopterus schreibersi fuliginosus)의 정자변태)

  • 손성원;이정훈;최병진;신화정
    • The Korean Journal of Zoology
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    • v.38 no.3
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    • pp.405-416
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    • 1995
  • The testis and the epididymis of sexually mature male bats were examined to investigate the process of spermiogenesis of Korean long-fingered bat (Miniopterus schreifersi fulignosus) using electron microscope. The ultrastructural findings were analysed on the basis of Lee's method (1992). Especially, we focused on the acrosome formation. The results are as follows: The spermiogenesis of the Korean long-fingered bat can be divided into ten phases on the basis of ultrastructural differentiation; three "Golgi" phases of early, mid and late stages, two "cap" and two "acrosome" phases respectively composed of early and late phases, one "maturation phase and two "spermiation" phases of early and late phases. The axoneme of sperm in the cauda epididymis is composed of nine outer dense fiber and a central singlet. The number 1, 5, 6, and 9 outer dense fibers are larger than others. In the Golgi phases, small vesicles are separated from Golgi vesicles and then appear to fuse into a large vesicle, and finally it contacd with the outerside of the nucleus. It suggests that proacrosomal material could be made in the cytoplasm before the Golgi vesicle formation and then it could be transferred into the Golgi vesicle and condensed more and more, and finally form acrosome, just as Lee;s suggestion (1992).m acrosome, just as Lee;s suggestion (1992).

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Utilization of LED Plant Factory for Medical Cannabis Cultivation (의료용 대마재배를 위한 LED 식물공장의 활용)

  • Hong, Chang-Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.7-7
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    • 2019
  • 우리나라에서의 대마는 마약류로 분류되어 기호용과 의료용 모두 법적인 제재를 받아왔으나 최근에 의료용 대마가 법적으로 허용이 되어 환자들에게 처방 받을 수 있게 되었다. 하지만 우리나라 사람들에게 대마는 환각성분을 가진 마약류로 깊이 인식되어 왔으며 의료용 대마에 대한 연구는 거의 전무한 실정이다. 노지재배에서 발생할 수 있는 범죄의 위험성, 자연교배에 의한 품질저하를 피할 수 있으며 재배조건(양액, 광조건, 광주기, 대기환경조성) 등을 제어할 수 있는 밀폐형 LED 식물공장은 의료용 대마 생산에 제일 적합한 모델이 될 수 있다. 식물체의 광합성은 가시광선 중 특정 파장 450nm 청색광과 660nm 적생광을 주로 이용하며 'LED (Light Emitting Diode) 발광다이오드'는 식물체의 광합성에 적합한 파장으로 광원을 조사할 수 있는 장점이 있다. 이러한 LED를 이용한 광합성은 식물체의 2차 대사물질 Anthocyanins, phenolic compounds 등의 향상과 생육에도 긍정적인 영향을 미치며 식물 뿐만 아니라 광합성을 하는 미세조류(micro algae)에서도 이용이 가능하며 해마토 코쿠스(Hematococcus)의 세포증식과 항산화물질 아스타잔틴(astaxathin)생산에 유용하게 쓰이고 있다. 최근 미국과 다른 나라에서도 기호용, 의료용 대마사업에 대한 관심이 높아지고 있으며 캐나다와 미국에서는 밀폐형 유리온실과 식물공장을 이용한 의료용 대마를 생산, 추출, 가공까지 하여 산업적인 영역을 넓히고 있는 실정이다. 특히, 실내재배에서는 광원이 필수적인 요소이며 식물생육에 선택적인 파장을 조사할 수 있는 LED와 재배조건을 정밀하게 제어할 수 있는 밀폐형 식물공장을 이용한 의료용 대마 생산에 대한 연구가 절실히 필요한 시점이다.

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