• Journal of Plant Biology
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• v.37 no.2
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• pp.183-193
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• 1994

인삼(Panax ginseng C.A. Meyer)의 뇌두 캘러스로부터 분리한 원형질체와 배양된 원형질체의 미세구조 변화와 원형질막 표면을 투과 및 주사전자현미경으로 조사하였다. 분리 직후의 원형질체에서는 캘러스세포에서보다 작은 액포들이 많이 형성되어 있었다. 또한 활면소포체의 수가 증가하였으며 이들은 원형질막과 평행으로 배열하였다. 활면소포체는 가끔 세포질을 둘러싸서 세포질분리체(cytosegresome)를 형성하였고, 이 구조는 세포질을 분해시킨 후 액포로 변화하기도 하였다. 배양된 원형질체에서는 분리 직후의 원형질체와 비교하여 조면소포체, 딕티오좀, 리보좀, 미토콘드리아, proplastid 및 액포 등의 수가 현저하게 증가하였다. 딕티오좀으로부터 많은 소낭들이 형성되었고 이들은 세포질 전반에 걸쳐 존재하였다. 때로는 소낭들이 원형질막 바깥으로 돌출되어 돌기를 형성하기도 하였다. 배양된 원형질체의 표면에는 섬유소로 구성되어 있을 것으로 추정되는 섬유상 구조들이 형성되어 있었다.

• Korean Journal of Plant Tissue Culture
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• v.21 no.3
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• pp.177-182
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• 1994

Cotyledon segments of korean ginseng produced somatic embryos when cultured on MS basal medium, whereas plumule or excised axis explants did not. histological examination revealed that the cells in proximal region of cotyledon turned meristematic and densely cytoplasmic was composed of smaller and more densely cytiplasmic cells than the subepidermal cells. however, in the case both epidermis and subepidermal cells were almost the same in size and cytoplasmic density, the embryo originated from multiple cells.

• Journal of the KSME
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• v.54 no.9
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• pp.35-38
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• 2014

이 글에서는 동물 세포의 3차원 배양 칩(생체 조직 칩)과 이에 쓰이는 다양한 생체재료들을 소개하고자 한다.

• The Korean Journal of Zoology
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• v.30 no.2
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• pp.177-192
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• 1987

온열처리가 근세포의 분화에 어떤 영향을 미치는 지를 알아보기 위하여 배양한 근세포에 여러가지 온열처리를 한 다음, 단백질 합성, 세포증식, 융합지수, creatine kinase(CK)의 활성 및 cholesterol 함량의 변화를 조사하였다. 배양한지 24시간지나 45$^{\circ}C$에서 1 hr의 온열처리를 하면 근세포의 융합과 CK 활성이 지연되었으며, 55시간지나 같은 온열처리를 하면 세포막내(세포내 양의 95% 이상)의 chloesterol 양이 일시적으로 증가함과 아울러 세포융합이 지연되었다. 그러나 세포증식은 대조군과 뚜렷한 차이가 없었다. 이상과 같은 실험 결과로부터 온열처리가 분화에 미치는 영향은 온열처리를 받게되는 근원세포의 분화정도에 따라 차이가 있으며 온열처리에 따른 chloesterol 양의 일시적인 증가가 근세포 융합에 영향을 미칠 수 있다는 가능성이 제시되었다. 한편, 근세포는 온열처리를 받으면 평소의 단백질 합성 수준이 떨어짐과 더불어 heat shock protein(HSP)합성이 증대 내지는 유도 되었으며 HSP 합성의 유도는 actinomycin D의 처리로 억제되었다. 또한 온열처리로 근세포는 열내성을 얻어 세포융하과 CK 활성은 동일한 온열처리를 4시간 간격으로 두번 주어도 한번 주었을 경우와 차이가 없었으며 두번째 온열처리에 의해서는 새로운 HSP 합성이 유도되지도 않았다.

• Journal of Life Science
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• v.17 no.5 s.85
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• pp.678-686
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• 2007

Human mesenchymal stem cells(hMSC), that have been reported to be present in bone marrow, adipose tissues, dermis, muscles and peripheral blood, have the potential to differentiate along different lineages including those forming bone, cartilage, fat, muscle and neuron. Therefore, hMSC are attractive candidates for cell and gene therapy. The optimal conditions for hMSC expansion require medium supplemented with fetal bovine serum(FBS). Some forms of cell therapy will involve multiple doses, raising a concern over immunological reactions caused by medium-derived FBS proteins. Previously, we have shown that hADSC can be cultured in human serum(HS) during their isolation and expansion, and that they maintain their proliferative capacity and ability for multilineage differentiation and promote engraftment of peripheral blood-derived CD34 cells mobilized from bone marrow in NOD/SCID mice. In this study we determined whether hADSC grown in HS maintain surface markers expression similar with cells grown in FBS during culture expansion and compared gene expression profile by Affymetrix microarray. Flow cytometry analysis showed that HLA-DR, CD117, CD29 and CD44 expression in HS-cultured hADSC during culture expansion were similar with that in FBS-cultured cells. However, the gene expression profile in HS-cultured hADSC was significantly different from that in FBS-cultured cells. Therefore, these data indicated that HS-cultured hADSC should be used in vivo animal study of hADSC transplantation for direct extrapolation of preclinical data into clinical application.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.365-368
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• 2003

Hair follicles develop as a result of epithelial-mesenchymal interactions between epidermal keratinocytes and dermal cells. Moreover hair follicles constitute multiple cells that influence hair follicle development and cyclic activity. We isolated some cells using explantation and enzymatic digestion method from human scalp hair follicles. So we could culture some follicular cells, such as outer root sheath (ORS) cells, dermal papilla (DP) cells, dermal sheath (DS) cells, matrix cells and melanocyte.

• KSBB Journal
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• v.7 no.2
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• pp.132-138
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• 1992

Sprial adaptation technique of conditioned media has been applied to cultivate human cell line which can not survive in a serum free mdium without adding any growth factors in basal medium Doubling time and scu-PA production from serum free adapted cells were 5 days and 890 (IU/mL), respectively in a T-flask, whose values were not much lower than the productivity of 1100(IU/mL) from 5% serum containing medium. It was required to use conditioned media for attaching cells on microcarriers when cells were inoculated into a spinner vessel. Then, cells could continuously grow in serum free medium with having specific growth rate of 0.106 (1/day) and specific scu-PA production rate of $1.58{\times}10_{-5}$(IU/cell/day) in batch cultivation.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.301-304
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• 2002

Perfusion culture strategies for high density culture of plant cell suspensions to enhance the productivity of extracellular polysaccharides were investigated. Angelica gigas Nakai cell suspensions were used to produce the extracellular polysaccharide and perfusion parameters were optimized to maximize the production. When the medium exchange was started at the fifth day after inoculation, the maximum cell concentration (23.8 g dry cell weight per liter) was achieved.

• Advances in pediatric surgery
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• v.15 no.1
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• pp.1-10
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• 2009

Recently, amniotic fluid has gained attention as one of the potential sources for cell therapy and tissue engineering because it has characteristics of multipotent stem cells. However, current knowledge about what types of cells are naturally found in amniotic fluid is still limited. In this study, we aimed to investigate whether human amniotic fluid contains cells that have characteristics of respiratory cells. Samples of human amniotic fluid (5 mL per sample) obtained from amniocenteses were cultured with small airway growth medium (SAGM). Cells were grown until the third passage and the presence of type II alveolar cells were characterized by inverted microscopy, immunofluorescence, and reverse transcription polymerase chain reaction (RT-PCR). On inverted microscopy, cultured cells showed typical polygonal and cobblestone-like epithelial morphology. The morphology of cells was not changed after selection and passing. Immunofluorescence analysis demonstrated that the isolated cells stained positive for surfactant protein C (SPC), specific marker for type II alveolar cells. Cells also stained positive for TTF-1 protein but negative for CD 31 and vimentin. RT-PCR analysis of cells showed expression of SPC mRNA. This study has demonstrated that respiratory cells can be isolated and identified from human amniotic fluid cultured in SAGM medium. Our results may provide the basis for further investigations of amniotic fluid.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.05a
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• pp.318-319
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• 2003

어류에서 바이러스성 질병이 중요하다는 인식이 증가하고 있으며 어류바이러스의 연구를 위한 세포주의 발달이 활성화되고 있다. 하지만상대적으로 해산유래의 세포 주 수는 많지 않다. 특히 유럽과 우리나라 남ㆍ서해안에서 고급어종인 감성돔은 림포 시스티스병, 이리도바이러스병 등의 바이러스성 질병이 발생하고 있다. 본 연구는 감성돔에서 유래한 세포주 개발을 목적으로 하였으며, 감성돔의 비장에서 유래한 세포를 초대배양 및 계대배양 하여 새로운 세포주를 확립하였으며, 일련의 실험을 실시하여 확립된 세포주의 특성을 확인하고 바이러스에 대한 감수성을 검사하였다. (중략)