• Parasites, Hosts and Diseases
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• v.33 no.3
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• pp.155-164
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• 1995

The location of actin and myosin of the several stages of Cwptosporinium parvum was observed. The tissue antigen of C. pcruum was prepared through immunosuppression of IgG mice with Depomedrol . The thin sectioned specimens, which were incubated with the IgG fraction of the rabbit polyclonal antibodies raised against chicken back muscle actin and bovine uterus myosin, were treated with 10 nm gold-conjugated goat anti-rabbit IgG, Electrodense particles were located mainly on the pellicles of all observed developmental stages of the parasites. The number of actin gold particles in the cytoplasm increased when the parasite was dividing actively as in case of meronts. Especially in macrogametocytes, a lot of actin and myosin particles were synthesized and storaged as amilopectin-like bodies. There were many actin gold particles along the microspikes of cytoplasmic membranes in various developmental stages. The actin and myosin observed in this study may play important roles to control the shape of the parasites and movement of cytoplasmic membranes as cvtoskeletal proteins.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.517-517
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• 2013

세포막지질의 산화는 심각한 세포막의 기능저하를 유발하고 심하면 세포를 죽음에 이르게하여 생물학적으로 중요한 지표이다. 세포막지질의 산화는 간접적인 화학적 방법으로 측정하거나, 지질을 추출해내어 질량분석기나 핵자기공명분광기 같은 물리적 방법으로 분석한다. 우리는 이온유도 이차전자 방출계수(${\gamma}$) 변화를 측정하여 세포막지질의 산화를 지질추출 없이 측정할 수 있는지 조사해 보았다. 세포막분리가 쉬운 적혈구를 모델세포로 사용하였고, 다양한 라디칼을 발생시키는 대기압 공기 DBD플라즈마 장치를 이용하였다. 적혈구를 플라즈마에 노출하는 시간으로 산화의 정도에 차이를 만들어 측정값과 비교하였다. ${\gamma}$값은 Auger의 중화이론에 바탕을 둔 이온유도 이차전자 방출빔(${\gamma}$-FIB)장비를 이용하여 측정하였다. 측정결과 적혈구가 산화됨에 따라서 ${\gamma}$값이 증가함을 볼 수 있었고, 동시에 workfunction값이 변화함을 보았으며, 그 결과를 화학적 방법과 비교해 보았다.

• Journal of Life Science
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• v.19 no.12
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• pp.1764-1768
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• 2009

This study was carried out to examine a concentration of steroid hormones and in vitro development of mouse embryos in culture supernatant of bovine granulosa cells (GC). To obtain the culture supernatant, granulosa cells were retrieved from mature follicles (6~15 mm diameter) and immature follicles (2~5 mm diameter) of bovine ovary and were cultured, respectively, in media of Ham's F-10 with 15% FCS for 16 days. Mature and immature granulosa cells formed their monolayers easily and showed similar growth patterns in culturing. There was no morphological difference between mature and immature granulosa cells. High levels of both progesterone and estradiol were detected in the culture supernatant of mature granulosa cells and immature granulsa cells, and the endocrine profiles of the two types of cells were similar. Progesterone secretion of granulosa cells was high in the late stage of culturing and estradiol secretion was high in the early stage of culturing. In vitro development rates of mouse embryos to morula, blastocyst and hatched blastocyst were significantly (p<0.05) higher in culture supernatant of mature granulosa cells (92.7%, 78.1% and 34.5%) and in culture supernatant of immature granulosa cells (96.4%, 78.5% and 26.8%) than in Ham's F-10 (86.7%, 41,7% and 13.3%). However, there was no difference between the culture supernatant of mature granulosa cells and the culture supernatant of immature granulosa cells in the development of embryos.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2001.05a
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• pp.162-163
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• 2001

기질농도, HRT, 온도, pH, $Ca^{2+}$ 농도변화에 따른 반응기의 생물막형성과 세포외고분자물질의 조성변화에 따른 기질 제거율을 살펴보았다. 그 결과 반응기의 초기 생물막 형성은 각 조건에 따라서 약간의 차이를 보였다. 반응기 운전 초기에 균에 의해 생산된 세포외고분자물질은 점액질의 형태로 세포벽에 부착되어 주위환경의 해로운 요인으로부터 세포를 보호하는 기능을 가지고 있어 생물막 형성을 촉진시키는 역할을 하였으나, 생물막이 안정화된 후에는 오히려 생물막의 산소투과와 영양분의 접촉을 떨어뜨리는 역할을 하여 기질 제거율을 저하시키는 작용을 하는 것으로 나타났다.

• The korean journal of orthodontics
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• v.30 no.2 s.79
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• pp.197-204
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• 2000

[$K^+$]-selective ion channels were studied in excised inside-out membrane patches from human osteoblast-like cells (G292). Three classes of $K^+$channels were present and could be distinguished on the basis of conductance. Conductances were $270\pm27\;pS,\;113\pm12\;pS,\;48\pm8\;pS$ according to their approximate conductances in symmetrical 140 mM KCl saline at holding potential of -80 mV It was found that the small conductance (48 pS) $K^+$channel activation was dependent on membrane voltage. In current-voltage relationship, small conductance $K^+$channel showed outward rectification, and it was activated by the positive potential inside the membrane. In recordings, single channel currents were activayed by a negative pressure outside the membrane. The membrane pressure increased $P_{open}$ of the $K^+$ channel in a pressure-dependent manner. In the excised-patch clamp recordings, G292 osteoblast-like cells have been shown to contain three types of $K^+$ channels. Only the small conductance (48 pS) $K^+$channel is sensitive to the membrane stretch. These findings suggest that a hyperpolarizing current, mediated in part by this channel, may be associated with early events during the mechanical loading of the osteoblast. In G292 osteoblast-like cells, $K^+$channel is sensitive to membrane tension, and may represent a unique adaptation of the bone cell membrane to mechanical stress.

• Journal of the Korean Society of Radiology
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• v.9 no.4
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• pp.187-195
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• 2015

The active transport characteristics of anions of cell membrane model which irradiated by $^{60}Co\;{\gamma}-ray$ was investigated. The cell membrane model used in this experiment was a sulfonated copolymerized membrane of poly(1-methyl-4-vinylpyridiniumiodide-co-divinylbenzene : MeVP-DVBI). First, the initial flux of $OH^-$ and $Cl^-$, $Na^+$ of membrane which was not irradiated was decreased with increase of thickness of membrane $80-200{\mu}m$, increased with increase of NaOH concentration 0-0.5mol/L and MeVP-DVBI concentration 20-80% was increased with initial flux of $OH^-$ and $Cl^-$, decreased with initial flux of $Na^+$. Second, the initial flux of membrane which was irradiated was less than that. And the driving force of pH of irradiated membrane was significantly increased more than membrane which was not irradiated. The initial flux of the $OH^-$ ion was decreased with increase of $H^+$ ion concentration. As selective transport of $OH^-$ and $Cl^-$ of cell membrane model were abnormal, cell damages were appeared at cell.

• Journal of Nutrition and Health
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• v.26 no.8
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• pp.1007-1018
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• 1993

이상의 모든 결과를 요약해 보면 어유의 경우 항혈전 효과에는 상당히 들기름과 함께 좋으나 막의 유연성과 다른 면에서 들기름은 항혈전과 세포막의 유연성 유지 등에서 상당히 유리한 기름으로 생각할 수 있다. 팜유는 우지나 참기름보다 항혈전 효과나 세포막 유연성 유지에서 좋으나 들기름보다는 떨어지는 것으로 생각할수 있다.

• Applied Microscopy
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• v.20 no.2
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• pp.81-101
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• 1990

Toad bladder의 상피수송(上皮輸送)을 분석(分析)하기 위하여 동결치환(凍結置換) 및 동결절단법(凍結切斷法)을 적용(適用)하여 전자현미경(電子顯微鏡) 관찰(觀察)을 실시하였다. 방광(膀胱)의 점막층(粘膜層)은 과립성세포(顆粒性細胞), 미토콘드리아가 풍부한 세포, 점막분필세포(粘膜分泌細胞) 및 기저세포(基底細胞)등 4가지 세포로 구성되어 있었다. 과립성세포(顆粒性細胞)는 점막표면적의 대부분을 점유하며 $Na^+$ 수송(輸送)에 주요한 역할을 하고, 정단부(頂端部)의 세포질에는 다수의 과립이 분포하며 정단세포막(頂端細胞膜)은 microvilli type I로 배열되어 있고, 표면에 glycoprotein을 함유하는 세포외막(細胞外膜)이 관찰되었다. 대조적으로 미토콘드리아가 풍부한 세포는 세포질 전역에 걸쳐 다수의 미토콘드리아가 분포해 있으며 주요 기능은 $H^{+},\;K^{+}$ 및 $HCO_{3}^{-}$ 분필수송(分泌輸送)에 관여할 것으로 생각되며 이들 수송상피(輸送上皮)는 정단부가 견고연접(堅固連接)으로 둘러 싸이고 기저세포막(基底細胞膜)은 인접세포와 서로 분리되므로 상피세포의 극성이 유지되며 정단부(頂端部) 세포막과 기저세포막의 수송특성(輸送特性)은 각기 다르다고 생각된다. 따라서 두꺼비 방광(膀胱)에서 상피수송(上皮輸送)은 세포 통과수송 및 세포간 분류수송 경로를 나타내고 있다. 한편 세포막 투과성(透過性)의 조절과 관련하여 동결절단(凍結切斷) 전자현미경 관찰에 의하면 forskolin에 촉진된 정단부(頂端部) 세포막 투과성의 변화는 세포막내(細胞膜內) 입자(粒子)의 분포와 밀접한 관계를 가지는 것으로 보인다. 특히 과립성세포(顆粒性細胞)에서 집단으로 관찰되는 세포막내(細胞膜內) 입자(粒子)는 forskolin에 유도된 정단세포막(頂端細胞膜) 투과성의 변화를 나타내주는 것으로 사료되나 이의 기능적 의미에 대하여는 연구가 더욱 필요하다고 본다.

• Journal of the Korean Society of Radiology
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• v.7 no.1
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• pp.85-91
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• 2013

The selective transport characteristics of $K^+$ and $Na^+$ of cell membrane model which irradiated by 60Co ${\gamma}$-ray was investigated. The cell membrane model used in this experiment was a Na+ type sulfonated copolymerized membrane of styrene and divinylbenezene. The initial flux of the ion was increased with increase of both $H^+$ ion concentration. In this experiment range(pH 0.5-3, temperature $15-65^{\circ}C$), first, the selectivity of $K^+$ and the ratio K+/Na+ of membrane which was not irradiated was about 1.06 - 1.13 and second, that of K+ and the ratio $K^+/Na^+$ of membrane which was irradiated was near about 0. And the driving force of pH of irradiated membrane was significantly increased about 4-5 times than membrane which was not irradiated. As selective transport of K+ and Na+ of cell membrane model were abnormal, cell damages were appeared at cell.

• Journal of the korean academy of Pediatric Dentistry
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• v.26 no.2
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• pp.218-231
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• 1999

Pyramidal cells in the hippocampal CA area were recorded from and filled with neurobiotin in anesthetized rats. The extent of their dendrites and the electropharmacological properties of membrane as well as the effect before and after neurobiotin injection were examined. Pyramidal cells had a high resting membrane potential, a low input resistance, and a large amplitude action potential. A afterhyperpolarization was followed a single action potential. Most pyramidal cells did not display a spontaneous firing. Pyramidal cell displayed weak inward rectification and anodal break excitation in response to negative current injection into the cell. Membrane properties of recorded neurons before and after neurobiotin injection with consecutive current injection were compared. Some properties were significantly increased after labelling(P>0.05); the duration and amplitude of sustained AHP, input resistance, and the number of action potentials for simultaneous intra- and extracellular stimulations. Neurobiotin-filled neurons showed pyramidal morphology. Cells were generally bipolar dendrite processes ramifying in stratum lacunosum-moleculare, radiatum, and oriens.