• Title/Summary/Keyword: 세대별 GC

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The chloroplast genome sequence of Viola kusanoana (큰졸방제비꽃(Viola kusanoana)의 엽록체 염기서열 분석)

  • Ah-reum Go;Ki-Oug Yoo
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.22-22
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    • 2021
  • 큰졸방제비꽃(Viola kusanoana)의 엽록체 DNA 염기서열을 밝히고자 차세대염기서열분석법(NGS)을 이용하여 분석하였다. 재료는 경상북도 울릉군 나리분지에 자생하는 개체의 잎을 사용하였다. 염기서열 분석결과, 총 길이는 158,644 bp 였고, GC함량은 36.3%로 분석되었다. 구간별로는 LSC (Large single copy)지역이 86,999 bp (GC content: 33.9%)였고 SSC (Small single copy)지역은 17,439 bp (GC content: 29.9%)으로 분석되었으며 IR (Invertied repeats)지역은 27,103 bp (GC content: 42.2%)로 확인되었다. 유전자는 protein coding gene 77개, tRNA gene 30개, rRNA 4개 등 총 111개로 이는 선행 연구된 제비꽃속 8개 분류군과 유전자의 순서와 방향이 모두 일치하였다. 이를 통해 제비꽃속의 엽록체 게놈의 유전자는 상당히 보존되어 있음을 확인하였다.

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Improving Haskell GC-Tuning Time Using Divide-and-Conquer (분할 정복법을 이용한 Haskell GC 조정 시간 개선)

  • An, Hyungjun;Kim, Hwamok;Liu, Xiao;Kim, Yeoneo;Byun, Sugwoo;Woo, Gyun
    • KIPS Transactions on Computer and Communication Systems
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    • v.6 no.9
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    • pp.377-384
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    • 2017
  • The performance improvement of a single core processor has reached its limit since the circuit density cannot be increased any longer due to overheating. Therefore, the multicore and manycore architectures have emerged as viable approaches and parallel programming becomes more important. Haskell, a purely functional language, is getting popular in this situation since it naturally supports parallel programming owing to its beneficial features including the implicit parallelism in evaluating expressions and the monadic tools supporting parallel constructs. However, the performance of Haskell parallel programs is strongly influenced by the performance of the run-time system including the garbage collector. Though a memory profiling tool namely GC-tune has been suggested, we need a more systematic way to use this tool. Since GC-tune finds the optimal memory size by executing the target program with all the different possible GC options, the GC-tuning time takes too long. This paper suggests a basic divide-and-conquer method to reduce the number of GC-tune executions by reducing the search area by one-quarter for every searching step. Applying this method to two parallel programs, a maximally independent set and a K-means programs, the memory tuning time is reduced by 7.78 times with accuracy 98% on average.

The Complete Chloroplast DNA Sequences of Viola selkirkii (뫼제비꽃(Viola selkirkii)의 엽록체 DNA 염기서열 분석)

  • Ah-Reum Go;Yun-Sun Lee;Kyung-Ah Kim;Kyeong-Sik Cheon;Ki-Oug Yoo
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.55-55
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    • 2020
  • 뫼제비꽃(Viola selkirkii)의 엽록체 DNA 염기서열을 차세대염기서열분석법(NGS)을 이용하여 분석하였다. 재료는 강원도 화천군 일산과 제주도 한라산의 2개체를 사용하였다. 분석결과, 염기서열의 길이는 일산의 뫼제비꽃이 156,774 bp (GC content: 36.30%), 한라산의 뫼제비꽃이 157,451 bp(GC content: 36.30%)로 한라산 개체가 길게 분석되었다. 구간별로 LSC(Large single copy)지역은 한라산 개체(85,950 bp)가 일산 개체(85,930 bp)보다 20 bp 길었으며, SSC(Small single copy)지역은 한라산 개체(17,261 bp)보다 일산 개체가 17,982 bp로 길게 분석되었다. IR(Inverted repeat)지역은 한라산 개체가 27,120 bp로 일산 개체(26,431 bp)보다 길게 분석되었다. 이러한 염기서열 길이의 차이는 종내 개체 간 빈번하게 발생하는 현상으로 IGS와 intron 구간에서 확인 된 단순반복서열의 일부 누락과 IR지역 내의 수축과 확장에 의한 것으로 판단된다. 뫼제비꽃 2개체의 엽록체 게놈을 구성하는 유전자 수는 총 111개로 동일하였으며, protein coding gene 77개, tRNA(transfer RNA) gene 30개, 그리고 rRNA (ribosomal RNA) gene 4개로 구성되어 있었다. 이는 기 발표된 엽록체 DNA 전체 염기서열이 밝혀진 제비꽃속 (Viola) 종류들과 동일한 결과이다.

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Sex Pheromone and Seasonal Occurrence of the peach leafminer, Lyonetia clerkella Linne (복숭아굴나방의 성페로몬과 성충 발생소장)

  • Yang Chang-Yeol;Jeon Heung-Yong;Kim Dae-Young;Kim Hyeong-Hwan
    • Korean journal of applied entomology
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    • v.45 no.1 s.142
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    • pp.25-30
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    • 2006
  • The female sex pheromone of the peach leafminer, Lyonetia clerkella Linne (Lepidoptera: Lyonetiidae), was analyzed by coupled gas chromatographic-electroantennographic detector (GC-EAD). GC-EAD analyses of pheromone gland extract revealed a single compound that elicited responses from male antennae. Retention time on DB-1 column of EAD-active compound was identical to that of synthetic (145)-14-Methyl-1-octadecene (14Sme-1-18Hy). In field tests, sticky traps baited with synthetic 14Sme-1-18Hy alone were highly attractive to male. Traps with 0.1 mg dose showed the lowest catches, but there were no significant difference in the numbers of moth caught in traps baited with doses of 0.5 and 1.0 mg. The results of the field assays for longevity of pheromone traps showed that effectiveness of lures maintained for at least 8 weeks under field condition. The attractiveness of 14Sme-1-18Hy was not affected by the addition of butylated hydroxytoluene (BHT) in lures as an antioxidant. Traps baited with 0.5 mg 14Sme-1-18Hy were successfully used to monitor L. clerkella male flights. Analysis of seasonal trap catches over two years showed that moth flight activity in peach orchards occurred over a period of seven months with six generations in Suwon.

THE COMPARATIVE STUDY FOR THE SHEAR BOND STRENGTH OF DENTAL ADHESIVES CURED WITH VARIOUS LIGHT SOURCES (다양한 광원으로 중합한 치과용 접착제의 전단강도에 관한 비교 연구)

  • Choi, Nam-Ki;Cho, Seong-Hoon;Kim, Seon-Mi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.38 no.1
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    • pp.33-41
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    • 2011
  • The objective of this study was to compare the shear bond strengths of five dentin adhesive systems cured with three different light curing sources. Seventy five noncarious permanent teeth were collected and stored in an 0.1% thymol solution at room temperature after extraction. The tested adhesives were: Adper Scotchbond Multi-purpose Plus Adhesive (SM) Adper Single bond 2 (SB), Clearfil SE Bond (SE), Adper Prompt L-Pop (PL), G-Bond (GB). And three light curing unit systems were used: Elipar Free light 2(LED), OptiLux 501 (Halogen), Flipo (PAC). For the shear bonding test, the labial and lingual surfaces of permanent teeth were used. To obtain a flat dentin surface, the labial and lingual surfaces of the teeth were sanded on SiO2 with number 600 grit and then divided into 15 groups of 10 surfaces each. All samples were theromocycled in water $5^{\circ}C$ and $55^{\circ}C$ for 1000 cycles. The results were as follows: 1. When cured with Freelight 2, the shear bond strength of SM was significantly higher than that of PL, GB (p<0.05), whereas no significant difference was found among those of any other bonding agents. 2. When cured with Optilux 501, the shear bond strength of SM was significantly higher than those of any other bonding agents (p<0.05), whereas no singnificant difference was found among those of andy other bonding agents. 3. When cured with Flipo, the shear bond strength of SM was significantly higher than those of SB, SE, GB (p<0.05), whereas no significant differences was found among those of any other bonding agents. 4. For comparison according to three different light cure unit system, except SB and GB, each three dentin bonding agents showed no significant difference. For SB, only Freelight 2 was significantly higher than the others, with no significant difference between Optilux 501 and Flip. For GB, Statistically significant difference was found only between Freelight and Flipo.