• Title/Summary/Keyword: 세균성

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PCR 방법에 의한 세균성 식중독균의 다중${\cdot}$동시 검출

  • Park, Ju-Yeon;Mun, Seon-Yeong;Lee, Gyeong-Ha;Nam, Jeong-Ok;Nam, Bo-Ra;Kim, Jin-Man
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2005.10a
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    • pp.346-349
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    • 2005
  • 병원성미생물을 분리${\cdot}$동정하는 방법으로 선택배지를 이용하는 검사방법은 4${\sim}$7일의 시간이 소요되는 단점이 있으며, 기존의 PCR방법은 대부분 단일 병원성 미생물만을 검출할 수 있는 반면 본 연구에서 사용된 시스템은 6종류의 세균성 식중독균을 한 번의 분석으로 확인할 수 있었기 때문에 비용과 분석시간을 대폭 절감하는 효과가 있었다. Fig. 1은 6종류의 세균성 식중독균이 혼합된 시료에 각각의 단일 프라이머를 이용하여 특이성을 확인한 결과이며, Fig. 2는 멀티플렉스 프라이머를 이용하여 각각의 세균성 식중독균 열 추출 시료에 대한 특이성을 확인한 결과이다. PCR 산물들이 사다리(ladder) 형태로 증폭됨으로써 동시에 6종류의 세균성 식중독균을 용이하게 확인할 수 있었다(Lane 1${\sim}$Lane 6). 또한 3종류의 세균성 식중독균이 혼합된 열 추출시료에서도 특이적인 PCR 증폭 산물들을 탐지할 수 있었다(Lane 10 & Lane 11).

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Distribution of Indicator Organisms and Influence of Storage Temperature and Period in Commercial Animal Foods (시판 동물성 식품의 오염지표세균 분포 및 저장온도, 기간별 오염지표세균의 변화)

  • 이용욱;박석기
    • Journal of Food Hygiene and Safety
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    • v.13 no.4
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    • pp.430-440
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    • 1998
  • The average number of total viable counts for the commercial pork tested was 19/g, coliform 1.8/g, psychrophilic bacteria 15/g, heterotrophic bacteria 12/g, fecal streptococcus 6.2/100 g, Pseudomonas aeruginosa 13/100 g and none of heat-resistant bacteria and Staphylococcus was detected. That for the commercial beef tested was 130/g, coliform 5.2/g, psychrophile 140/g, heterotroph 28/g, Staphylococcus 1.2/g, fecal streptococcus 9.5/100 g, Pseud. aeruginosa 1.9/100 g and heat-resistant bacteria was not detected. That for the commercial chicken tested was 8800/g, coliform 53/g, psychrophile 4600/g, heterotroph 4700/g, fecal streptococcus 9.9/100 g, Pseudo aeruginosa 2.5/100 g. That for milk was 4700/ml, psychrophile 120/ml, heterotroph 420/ml and the others were not detected. That for the commercial cheese was 3.2/g, psychrophile 2.3/g, heterotroph 1.6/g, Staphylococcus l/g, fecal streptococcus 9.1/g. That for fermented milk was $10^{7}/ml$, heatresistant bacteria $10^{6}/ml$, fecal streptococcus 2400/100 ml, lactobacillus $3.2{\times}10^{15}/ml$, in accordance with lactic acid bacteria and the others were not detected. There was not detected any indicator organisms from ham, sausage, butter, eggs and quails in the commercial fooods tested. SPC, coliform, psychrophile and heterotroph in commercial meats stored at $10^{\circ}C$ were increased rapidly as time goes on but heat-resistant bacteria, staphylococcus, fecal streptococcus and Pseudo aeruginosa were constant. At $20^{\circ}C$, SPC, coliform, psychrophile, heterotroph and fecal streptococcus were the highest at 7 days and heat-resistant bacteria, staphylococcus and Pseudo aeruginosa were increased a little. At $30^{\circ}C$, all indicators were increased rapidly for 3 and 7 days and then decreased rapidly. All indicator organisms were increased at the level of 10/g for 14 days in meat products stored at $10^{\circ}C$, but SPC, psychrophile and heterotroph in meat products stored at $20^{\circ}C$ were increased at the level of $lO^5/g$. It showed that the indicators in meat products stored at $30^{\circ}C$ had a tendency to increase at the level of $10^{2}/g$ relative to those stored at $20^{\circ}C$. SPC, psychrophile and heterotroph in milk stored at $10^{\circ}C$ increased up to the level of $10^4/ml$, but coliform, staphylococcus, fecal streptococcus and Pseudo aeruginosa were not detected. As stored at $20^{\circ}C$ and $30^{\circ}C$, they were increased rapidly for 1 or 3 days and then constant for a long time.

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Evaluation of Bacteriological Safety in Deep Confined Aquifer (심층피압지하수에서 세균군집의 분석에 의한 분변성 오염 평가)

  • 조장천;김상종
    • Korean Journal of Microbiology
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    • v.35 no.1
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    • pp.47-52
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    • 1999
  • The study on the bacterial distribution was performed to evaluate the bacteriological safety in 4 deep confined aquifers drilled for drinking. All the investigated aquifers were oligotrophic, howevec bacterial densities and activities and the number of indicator bacteria in C-2 boreholes were much higher than those in other boreholes. Fecal pollution was observed in 3 boreholcs except C-1 borehole. The ratio of gram positive bacteria of the C-2 borehole was much lower than those 01 another boreholes. The numbers of total colifonns, fecal coliforms, and fecal Slreptococcus in the C-2 boreholes were 61, 33, 55 CFU (250 ml)$^{-1}$

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Effects of Seeding on the Microbial Changes During Thermophilic Composting of Food Waste (종균 첨가가 음식물 쓰레기 퇴비화 미생물에 미치는 영향)

  • Choi, Min-Ho;Chung, Yoon-Jin;Park, Yun-Hee
    • Journal of the Korea Organic Resources Recycling Association
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    • v.4 no.1
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    • pp.1-11
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    • 1996
  • The effect of seeding on the thermophilic composting of food waste was investigated with selected strains of yeast, thermophilic bacteria and the mixed strains of yeast and bacteria in the lab-scale composter at $50^{\circ}C$. The results demonstrated that in all case the growth of yeasts preceded the growth of thermophilic bacteria by one or two days. The seeding of bacteria alone was ineffective for thermophilic bacterial development and the rapid increase of total thermophilic bacterial count was obtained by mixed seeding of yeasts and bacteria. Moreover, the bacterial growth was accelerated by the addition of yeasts alone. It was revealed that the yeasts removed organic acids from the food waste at the initial period and consequently induced the growth of thermophilic bacteria. The results presented in this paper indicate the indispensable role of yeasts for thermophilic process of food waste composting.

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Identification of differentially expressed proteins in the bacterial biofilm (세균성 바이오필름-특이 발현 단백질의 규명)

  • Kang, Chi-Dug;Choi, Jeam-Il
    • Journal of Periodontal and Implant Science
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    • v.35 no.2
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    • pp.271-275
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    • 2005
  • 본 연구는 치주낭에 biofilm형태로 부착되어 질환을 유발시키고 항생제 빚 항균제에 저항을 일으키는 세균 독성요소를 규명하기 위해 시행된 기초연구이다. 치주질환의 주 병원균의 하나인 Porphyromonas gingivalis 381 biofilm의 세포외막에 특이하게 발현되는 단백질을 규명하기 위한 기초적인 자료를 얻기 위해 시행하였다. Porphyromonas gingivalis 381을 통상적인 세균 배양용 broth를 사용하여 혐기성 세균 배양기로 24시간 배양한 것을 대조군으로 하고, tissue culture plate를 이용하여 혐기성 배양조건 하에서24시간동안 biofilm을 형성하여 실험군으로 설정하였다. 세균을 수획하여 세포외막을 분리하고 isotonic isoelectric focusing을 시행한 결과 주로 약 20-30 kilodaltons에 해당하는 수종의 세균세포막 단백질이biofilm으로 배양한 세균에서 더 상승적으로 발현됨이 관찰되었고, 상이한 수종의 단백질도 planktonic culture broth로 배양한 세균에서 다 상승적으로 발현됨을 관찰할 수 있었다. 이것은 세균의 배양조건과 환경에 따라 그 외막 단백질이 서로 다르게 발현됨을 입증하는 기초적인 자료로서 향후 단백질의 동정과 성격을 규명하는 근간 실험으로 추진할 계획이다.

Characteristics of Heterotrophic Bacteria and Their Relationships with Environmental Parameters in Naktong Estuary (낙동강 하구 생태계의 종속영양세균의 특성 및 환경요인과의 관계)

  • 권오섭;하영칠
    • Korean Journal of Microbiology
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    • v.26 no.3
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    • pp.256-261
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    • 1988
  • Samples from Naktong Estuary had been taken for the characterization of heterotrophic bacterial communities and of the effects of environmental factors on their distribution in estuarine ecosystem. Bacterial communities isolated from seawater region were composed of more euryhalone groups than those from freshwater region, and the bacterial communities of summer were composed of more eurythermal groups than those of winter. Bacterial commnities became more diverse by the input of allochthonous bacteria from terrestrial and freshwater ecosystem, but less diverse by worse environmental conditions such as nutrient load, high salinity, low temperature, and so on.

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The Causative Organisms of Pediatric Bacterial Meningitis: A Single Center Experience from 1996 to 2005 (단일기관에서의 소아 세균성 수막염 원인균 분포 (1996~2005))

  • Lee, Taek Jin;Chun, Jin Kyong;Kim, Dong Soo
    • Pediatric Infection and Vaccine
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    • v.13 no.2
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    • pp.124-129
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    • 2006
  • Purpose : We investigated the causative organisms of bacterial meningitis by age distribution from 1996 to 2005. Methods : Retrospective data were obtained from the medical records with diagnosis of bacterial meningitis or neonatal meningitis from 1996 through 2005. A case was defined by isolation of organism or detection of its antigen by latex agglutination from cerebrospinal fluid. Results : A total of 46 cases(27 neonates and 19 children) were identified. 15 of 27 episodes(55.6%) of neonatal meningitis had a concomitant-positive blood culture. Group B streptococci were the most common bacterial causes of neonatal meningitis(44.4%). Nine of 12 episodes(75.0%) were late-onset infections in neonatal meningitis caused by group B streptococci. 16 of 19 children(84.2%) with bacterial meningitis beyond the neonatal period were younger than 5 years of age(median age, 23 months). Of 19 cases, 8 infections were with Streptococcus pneumoniae, 8 were with Haemophilus influenzae and 3 were with Neisseria meningitidis. Since 2001 there was no case of meningococcal meningitis in this study. Conclusion : In neonates group B streptococci are the most common causative organisms of bacterial meningitis, especially late-onset infections. In infants and young children, the predominant causes of bacterial meningitis are H. influenzae and S. pneumoniae; meningitis caused by the former are likely to decrease after the introduction of the conjugate vaccine for H. influenzae type b.

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An Explorator Spatial Analysis of Shigellosis (세균성 이질의 탐색적 공간분석)

  • 박기호
    • Journal of the Korean Geographical Society
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    • v.34 no.5
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    • pp.473-491
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    • 1999
  • 세균성 이질은 국내 제1종 법정 전염병으로 분류되어 관리되고 있는 질환으로서 1998년 이후 그 발병 사례가 급속히 증가하고 있다. 본 연구는 1999년 3월 부산시 사상구에서 집단 발병한 세균성 이질을 대상으로 하여, 각 환자들의 발병 시점과 장소의 분포패턴에 대한 지리학적 고찰을 목적으로 한다. 환자분포의 특징적 공간패턴과 그들의 시계열적 확산 양상 등을 탐색하기 위한 방법론은 보건지리학과 지도학 및 공간통계학에 기반을 둔 공간분석기법을 중심으로 설정하였다. 분석자료는 해당 지역의 수치지형도, 지적도, 인구 센서스 자료를 포함한 GIS 데이터베이스로 구축되었다. 인구분포를 감안한 밀도구분도를 바탕으로 개별환자의 위치자료와 동 단위로 집계된 자료를 자료의 형태에 따라 분석기법을 달리하였으며, 환자 발생 밀도, 상대적 위험지수 등을 지도화하여 역학자료의 시각적 통계적 분석을 수행하였다. 환자분포의 공간적 중심위치와 분산의 변화 등 기술적 통계분석과 함께 제1차 공간속성을 커널추정법으로 찾아보았다. 이와 더불어 ‘공간적 의존성’과 관련된 제2차 공간속성은 K-함수와 시뮬레이션을 통해 분석하여 군집성 등이 통계적으로 확인되었다. 본 연구를 통해 역학조사시 GIS의 활용사례가 제시되었으며, 모집단 인구를 고려한 확률지도 작성 기법과 다양한 데이터 가시화 방법, 그리고 시계열별 발생 환자들의 지리적 변이를 분석 하는데 따르는 문제들이 논의되었다.

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Studies on the Bacterial Soft Rot Disease of Lilliaceae Crops 1. Identification of Erwinia Causing Soft Rot of Onion (백합과(百合科) 채소(菜蔬)의 세균성(細菌性) 부패병(腐敗病)에 관(關)한 연구(硏究) 1. 양파 부패(腐敗)를 일으키는 Erwinia 속(屬) 세균(細菌)의 동정(同定))

  • Han, Kwang Sup;Choi, Jae Eul
    • Korean Journal of Agricultural Science
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    • v.16 no.1
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    • pp.19-25
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    • 1989
  • Twelve isolates of bacteria obtained from infected onions were classified into genus Erwinia based on diagnostic characteristics. Of twelve isolates studied, five were identified as E. carotovora subsp. carotovora, six as E. rhapontici and one as E. chrysanthemi on the bases of bacteriological properties. Symptoms caused by the genus Erwinia were different to be identified among the species. Therefore, we propose to name the disease of onion caused by E. carotovora subsp. carotovora, E. rhapontici and E. chrysanthemi as "bacterial soft rot of onion".

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Usefulness of Low Risk Criteria for Serious Bacterial Infection Among Febrile Infants Younger than Three Months of Age (생후 3개월 이하의 발열이 있는 환아에서 세균성 감염의 예측을 위한 저위험 예측기준의 유용성)

  • Kim, So Hyun;Jung, Ji Ah;Kim, Hae-Soon;Yoo, Eun Sun;Sohn, Sejung;Seo, Jeong Wan;Lee, Seung Joo
    • Clinical and Experimental Pediatrics
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    • v.45 no.8
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    • pp.967-972
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    • 2002
  • Purpose : A retrospective study was undertaken to evaluate the usefulness of low risk criteria for identifying febrile infants younger than three months unlikely to have serious bacterial infection. Methods : We conducted a retrospective study of 527 infants younger than three month with a axillary temperature ${\geq}37.4^{\circ}C$. If they met the following all four criteria, appear well, WBC $5,000-20,000/mm^3$, urine stick WBC(-) and nitrite(-), CSF WBC < $10/mm^3$, they were considered at low risk for serious bacterial infection(SBI). SBI was defined as a positive culture of urine, blood, or cerebrospinal fluid. The sensitivity, specificity, negative predictive value and positive predictive value of the low risk criteria were calculated. Results : Of 527 febrile infants, 110(21.0%) had serious bacterial infections. The 2.7% who met the low risk criteria had SBI and negative predictive value was 97.3%. SBI was diagnosed in 103 infants(38.6%) who didn't meet the low risk criteria including urinary tract infection(78.6%), most commonly, bacteremia(16.5%), bacterial meningitis(8.7%), Salmonella gastroenteritis(1%), osteomyelitis( 1%), septic arthritis of hip joint(1%). There were no differences in the sensitivity and negative predictive value according to the monthly-age-group. Conclusion : This low risk criteria to identify infants unlikely to have SBI early is available, however low risk infants must be carefully observed.